Property | Value |
Name | CORRELATION OF CEFOXITIN DISC DIFFUSION TEST AND OXACILLIN DISC DIFFUSION TEST FOR DETECTING mec A MEDIATED OXACILLIN RESISTANT STAPHYLOCOCCUS AUREUS |
Description | Research article:- *Madhusudhan NS1, Deepa S2, Shoba DN3 1.Assistant Professor, Department of Microbiology, Indira Gandhi Medical College and Research Institute Puducherry ,India. 2.Assistant Professor , Department of Microbiology, Mysore Medical College, , Karnataka, India. 3.Professor and Head, Department of Microbiology, Karnataka Institute of Medical Sciences, Hubli, Karnataka, India.
Abstract:- Background and objectives: The incidence of methicillin resistance Staphylococcus aureus in hospitals range from 15% to 70% in various parts of India. Phenotypic detection of MRSA has been problematic ever since its discovery in 1962. Test conditions like temperature, disk potency, salt concentration and time of incubation play very important role in the detection of methicillin resistance. With this background simple disk diffusion test which can be used simultaneously with other antibiotics is being evaluated. Methods: 100 oxacillin resistant Staphylococcus aureus strains were selected. Cefoxitin disc diffusion test was performed by on MHA as per CLSI guidelines. mecA gene was detected for these strains using PCR. Results: Among 100 oxacillin resistant staph aureus strains, 84 were cefoxitin resistant and 73 were mecA positive. Conclusion: By using oxacillin disc diffusion test 27 were showing false positive and by using Cefoxitin, 11 were showing false positive. In summary PPV for cefoxitin disc diffusion (86%) to detect mecA mediated resistance is higher when com-pared to oxacillin disc diffusion test (73%). Routine susceptibility testing method for Staphylococcus aureus could replace oxacillin disks by cefoxitin discs.
Key words :- Cefoxitin, Disk Diffusion Test, MRSA, Staphylococcus aureus. |
Filename | Madhusudhan N S et.al..pdf |
Filesize | 83.64 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 09/16/2011 00:00 |
Viewers | Everybody |
Maintained by | Editor |
Hits | 3055 Hits |
Last updated on | 09/26/2011 03:02 |
Homepage | |
CRC Checksum | |
MD5 Checksum |