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Name | Determination of co-enzyme Q10 (ubiquinone Q10) in Africa catfish (Clarias gariepinus) liver oil |
Description | Research article:-Paediatrics Caroline .A. Okoli1* & Gregory .E. Anekwe2 1Department of Paediatrics, University of Jos, Nigeria. 2Department of Biochemistry University of Jos, Nigeria. Abstract: Background: In Nigeria, there is a very limited documentation on nutrient composition of fish species and the Africa catfish in particular. There is no documented data on identification of coenzyme Q10 in Africa catfish (Clarias gariepinus) liver oil. Africa catfish (Clarias gariepinus) is more common and cheaper than some other fishes rich in coenzyme Q10. Thus, identifying this nutrient in this fish could help in proper dietary planning, thereby ensuring better health. Method and Result The lipid fractions of the purified supernatant from centrifugation of 250g of 400 Africa catfish livers in 0.02M phosphate buffer pH7.0 were extracted using chloroform-methanol solution and purified by Folch wash giving a concentration of 100μg of lipids per μl. The lipid fraction was fractionated into neutral lipid and polar lipids by silicic acid chromatography and saponified using petroleum ether layer. Alumina column chromatography was used to fractionate and isolate the non-saponifiable parts including coenzyme fraction and their provisional identification was achieved by silica gel TLC and comparing of Rf values with that of reference standards after staining with iodine. The Rf of coenzyme fraction was 0.19.Confirmatory identification of coenzyme was by spectrophotometer. Coenzyme fraction and ubiquinone standard had peak absorption at 328nm and the concentration of ubiquinone in the coenzyme fraction was 0.6mg/ml Conclusion: Coenzyme Q10 is present in the liver oil of Africa catfish (Clarias gariepinus).
Key words: Co-enzyme Q10, ubiquinone Q10, Africa catfish (Clarias gariepinus), liver oil, Nigeria.
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Copyright © 2013 Caroline. A Okoli & Gregory. E Anekwe. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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