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Original Article
Rahul Ravindra Bagul1*, Utkarsha Joshi2, Vikram Kakatkar3, Sanjay Deo4
1 Associate Professor, Department of Orthopaedics, Padmashree Dr. D.Y. Patil Medical College, Hospital and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India
2 Assistant Professor, Department of Orthopaedics, Padmashree Dr. D.Y. Patil Medical College, Hospital and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri,Pune, India
3 Senior Resident, Department of Orthopaedics, Padmashree Dr. D.Y. Patil Medical College, Hospital and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India
4 Professor, Department of Orthopaedics,Padmashree Dr. D.Y. Patil Medical College, Hospital and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India
Address reprint requests to
*Dr. Rahul R. Bagul, Department of Orthopaedics,Padmashree Dr. D.Y. Patil Medical College, Hospital and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India
Article citation: Rahul B, Utkarsha J,Vikram K, Sanjay D. Comparative study of management of proximal humerus fractures in elderly by conservative method Versus operative locking compression plate. J Pharm Biomed Sci 2015;05(11):831–838.
Abstract:
Background Proximal humeral fracture in patients more than 65 years old, represent the third most common fracture. Treatment of proximal humerus fractures, especially displaced fractures, remains controversial. Conservative treatment has been preferred for most of the undisplaced or minimally displaced fractures. Over the years, availability of improved fixation devices, popularised the treatment of these fractures by open reduction and internal fixation. Operative treatment of proximal humerus fractures poses a challenge because of complications like malunion, non-union and avascular necrosis.Objective To study the role of conservative treatment and operative treatment by locking compression plate in the management of these fractures. To compare the results ofconservative management Versus locking plate osteosynthesis. To EVALUATE the results of treatment in terms of clinical and radiological union as well as functional outcome.
Materials and methods In the present case study, we report our experience in 60 cases in whom comparative study of management of proximal humerus fractures in elderly by conservative method Versus operative locking compression plate was done.Results As measured by Neer’s shoulder score, out of the 60 cases in our study, 8 (13.33%) had excellent functional outcome out of which 3 were treated conservatively and 5 were treated operatively, 29 (48.33%) had satisfactory outcome out of which 14 were treated conservatively and 15 were treated operatively, 19 (31.67%) had unsatisfactory outcome out of which 10 were treated conservatively and 9 were treated operatively, and 4 (6.67%) cases were failures out of which 3 were treated conservatively and 1 were treated operatively.
KEYWORDS proximal humerus fracture, old age, locking compression plate, conservative treatment
Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None
Competing interest / Conflict of interest:
The author(s) have no competing interests for financial support, publication of this research, patents, and royalties through this collaborative research. All authors were equally involved in
discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Research Article.
Erika Teresa Quintana1*,Diana Andrea Gil-Rivera1,Amanda Alejo-Viderique1,Oliver López-Villegas1, Luis Ángel Maldonado2,3
1 Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN),México DF, México
2 Facultad de Química, Universidad Nacional Autónoma de México (UNAM), México DF,México
3 Rectoría – Secretaría General, Universidad Autónoma Metropolitana (UAM), México DF, México
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*E. T. Quintana,
Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), México DF, México
Article citation: Quintana ET, Gil-Rivera DA, Alejo-Viderique A, López-Villegas O,Maldonado LA. Evaluation of the antifungal and antiyeast activities from recently isolated Streptomycetes. J Pharm Biomed Sci 2015;05(11):867–876. Available at www.jpbms.info
Abstract: The indisputable role of Actinobacteria (aka “actinomycetes”) in the pharmaceutical industry is represented by its innate capability to produce antibiotics, but little is known about the antifungal compounds they could also be producing. Among the Actinobacteria,the genus Streptomyces produces antagonistic molecules against several pathogenic fungi and search/discovery programs should be revisited due to novel and/or poorly studied fungal emerging diseases in humans and plants. In humans, this is certainly important because novel antifungal therapies are among the most challenging problems in intensive care medicine. The present study focused on the isolation and identification of novel organisms of the genus Streptomyces and their evaluation for antifungal activities.
One hundred actinomycetes were isolated from Mexican soil samples and identified by using two pairs of specific primers: (a) a pair of primers for the class Actinobacteria and (b) for the family Streptomycetaceae; all the isolates selected were found to contain LLA 2pm in their cell walls, a-wall chemical marker for Streptomyces. The isolates were thenassigned to 38 multimembered groups on the basis of their morphological properties and one representative of each subgroup tested on in vitro antibiosis methods for
antifungal–antiyeast activities. 97.4% and 81.6% of the isolates showed activity against the type strains of Aspergillus niger and Candida albicans, respectively. 16S rRNA gene sequencing of five isolates showed that they are closely related among each other and felt in the Streptomyces griseus subclade which is highly heterogeneous; the isolates may well represent novel species showing both antifungal and antiyeast activities, a property not fully explored for members of that Streptomycetes 16S rRNA gene subclade.
KEYWORDS Actinomycetes, Streptomyces, antifungal activities, Aspergillus niger,Candida albicans
Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: ETQ CONACyT Jóvenes Talentos SIP-2015-RE/057-CONACYT C110/2015 and SIP20150292.
Research article
Kun Zhang1,Jiayu Gao2,Zaijun Zhang3*
1Institute of New Drug Research and Guangdong PROVINCE Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine, Jinan University College of Pharmacy,Guangzhou 510632 China
2Institute of New Drug Research and Guangdong PROVINCE Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine, Jinan University College of Pharmacy, Guangzhou 510632, China
Abstract:
This study was designed to investigate the difference between three 6-hydroxydopamine(6-OHDA)-induced rat PD models. Those rats model were established by stereotaxic unilateral 6-OHDA injection into different parts of nigrostriatal pathway including the striatum (ST), the substantia nigra pars compacta (SNPc) and the Ventral tegmental area (VTA) in left side of rat brain. To detect pathological change in the unilaterally lesion 6-OHDA rats,the intact hemisphere work as a internal control. Immunohistochemical staining was used to EVALUATE the expression of tyrosine hydroxylase (TH) in substantia nigra pars compacta, as indication of injure of dopaminergic neuron. Remarkable TH-postive neuron loss was found in substantia nigra pars compacta in all 6-OHDA injected groups.the steep depletion of the content of dopamine and its metabolites of in striatum was also observed in all 6-OHDA injected groups. The 6-OHDA-impaired rats demonstrate a decrease of body weight compared to sham group rats. Behavioral assessments of motor impairments in the unilateral 6-OHDA rat model were done by apomorphine-induced rotation tests. in apomorphine-induced contralateral rotations test,the ST injection group show increasing rotation from the 2th week to the 4th week and high success rate of modeling; SNpc group and VTA + SNpc groups showed stable rotations from the 2nd week but low success rate. These results suggested that ST group has higher success rate and is more practicable than the other two groups.
Article citation: Zhang K, Gao JY, Zhang ZJ. EVALUATION OF PARKINSON’S DISEASE IN 3 RAT MODELS INDUCED BY THREE DIFFERENT UNILATERAL INJECTIONS OF 6-HYDROXYDOPAMINE. J Pharm Biomed Sci 2015;05(11):842–847.Available at www.jpbms.info
Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None.
Competing interest / Conflict of interest:
The author(s) have no competing interests for financial support, publication of this research,patents, and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Case report
Renuka Jadhav*, Shradha Lohiya, Sharad Agarkhedkar, Anita Lohakare, Shradha Salunkhe
Department of Pediatrics. Dr. D. Y. Patil Medical College, Hospital and Research Centre (D. Y. Patil University Pune) Pimpri, Pune 411018, India
Abstract:
Background Dengue haemorrhagic fever (DHF), a potential lethal complication affects the Asian countries and has become a leading cause of hospital admissions and death among children. Dengue with its severe manifestations of DHF and dengue shock syndrome (DSS) has emerged as a major public health problem of international concern.
Aims To identify the predictive factors for spontaneous bleeding manifestations in dengue illness in children. To determine the utility of haematological parameters as diagnostic markers of dengue haemorrhagic fever.
Materials and Methods This is a prospective, cross-sectional study. Eighty patients (1–12 years) with proven diagnosis of dengue by NS1 were enrolled. Patients were DIVIDED into two groups: DHF group and DF group. A detailed history, clinical examination, with laboratory parameters was done in all patients. Data was analysed using SPSS Version 7 by applying the appropriate tests.
Results Dengue showed a seasonal trend, maximum number of cases (44 cases) were seen in September and October in 2012. Fever (100%), rash (62.5%), vomiting (60%), and abdominal pain (31.25%) were common features. Bleeding manifestations were petechiae and purpura 60.62%, melena was 40.63%, epistaxis was 15.63%, haematemesis was 3.12% and subconjuctival haemorrhage was 3.12%. Abdominal pain (31.25%), tender hepatomegaly (57.5%), and positive tourniquet test were significantly associated withspontaneous bleeding. Low platelet count was significantly associated with spontaneous bleeding but thrombocytopenia alone could not predict spontaneous bleeding.
Conclusion Clinical criteria as such as abdominal pain, tender hepatomegaly, and positive tourniquet test along with the laboratory parameters such as haemoconcentration, low platelet count, abnormal serum levels of liver enzymes, and prolonged prothrombin time were significantly associated in predicting spontaneous bleeding and these predictors of spontaneous bleeding can help the clinician to anticipate the problem, prognosticate,decide the need of referral to higher centre for monitoring, managing and blood product transfusion when required, thus limiting the morbidity and mortality.
KEYWORDS dengue, dengue haemorrhagic fever (DHF), dengue shock syndrome (DSS)
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Dr. Renuka Jadhav, Professor, Department of Pediatrics Dr. D. Y. Patil Medical College, Hospital and Research Centre (D. Y. Patil University Pune), Pimpri, Pune 411018, India
Article citation: Jadhav R, Lohiya S,Agarkhedkar S, Lohakare A, Salunkhe S. Study of dengue fever with special reference to predictors of spontaneous bleeding in dengue infection. J Pharm Biomed Sci 2015;05(11):851–856. Available at www.jpbms.info
Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None.
Competing interest / Conflict of interest:
The author(s) have no competing interests for financial support, publication of this research,patents, and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Case report
Anjana Chaudhary1,Sunita Chandrakar2,Diwakar RK3*,Meena Naik1
1 Department of Obstetrics & Gynaecology,C. M. Medical College & Hospital, Kachandur, Durg (C.G.), India
2 Department of Pharmacology, C. M.Medical College & Hospital, Kachandur, Durg (C.G.), India
3 Department of Radiodiagnosis, C. M.Medical College & Hospital, Kachandur, Durg (C.G.), India
Abstract:
Although the incidence of allergic reactions to local anaesthetics has decreased, they are still documented. Allergic reactions may consist of cutaneous lesions, urticaria, oedema or anaphylactic reactions. Severe hypersensitive reaction to local infiltration resulting in thin, charred and parchment-like skin with subsequent granuloma formation requiring debridement has not been reported earlier. We report a case of severe local skin reaction to lignocaine which is known to contain methylparaben as a preservative.
KEYWORDS hypersensitivity reaction, local anaesthetic agent, methylparaben
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*Diwakar RK, Department of Obstetrics & Gynaecology, Radio-diagnosis, C. M. Medical College & Hospital, Kachandur,Durg (C.G.), India
Article citation: Chaudhary A, Chandrakar S, Diwakar RK, Naik M. Severe skin hypersensitivity reaction to local infiltrating anaesthetic agent. J Pharm Biomed Sci2015;05(11):848–850.Available at www.jpbms.info
Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None.
Competing interest / Conflict of interest:
The author(s) have no competing interests for financial support, publication of this research,patents, and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.