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Original article
Blau Olga1,Bulegenova Minira2*,Karazhanova Meryert3,Nurpisova Dina4, Jolbaeva Kaliyash5, Makhneva Anna6, Boranbaeva Riza7
1Professor, Head of Genetic Laboratory, Charite University, School of Medicine, Germany Professor,2 Specialist, 3,4 and Head,7 Department, Scientific Center of Pediatrics and Children Surgery, Kazakhstan
5,6Cytogenetic Laboratory, Scientific Center of Pediatrics and Children Surgery, Kazakhstan
The name of the department(s) and institution(s) to which the work should be attributed:
Charite University, School of medicine, Germany. Scientific Center of Pediatrics and Children Surgery, Kazakhstan
Address reprint requests to
*Minira Bulegenova, Professor and Head of Laboratory Department, Scientific Center of Pediatrics and Children Surgery, Al Farabi av., Kazakhstan
Article citation: Olga B, Minira B, Meryert K, Dina N, Kaliyash J, Anna M, Riza B. Comparative investigation of conventional cytogenetic and fluorescence in situ hybridization in children with acute lymphoblastic leukemia. J Pharm Biomed Sci 2015;05(11):884–889. Available at www.jpbms.info
Abstract:
Acute lymphoblastic leukemia (ALL) is the most common malignancy in childhood. Chromosomal aberrations are independent prognostic factors. Conventional cytogenetics is routinely used in the initial assessment. Nevertheless, karyotyping is often hampered by low mitotic index of malignant cells, poor chromosomal morphology and difficulties in interpretation of chromosome rearrangement. Interphase FISH provides an alternative approach to detect abnormalities in nondividing cells and also is essential for the identification of cryptic abnormalities. In the present study we analysed 56 children with ALL using both cytogenetic and FISH techniques to determine diagnostic accuracy of the both methods. FISH probes for AML1-ELN, BCR-ABL, and MLL rearrangement were used. Karyotyping was successful in 77% of cases. Cytogenetic study discovered abnormalities in 51% from succeeded karyotyping.
FISH revealed chromosomal aberration in 62.5%. FISH confirmed all cases with clonal aberrations, observed with conventional cytogenetics. Among patients with normal karyotype, 24% were detected to have clonal aberrations by FISH. Also, FISH analysis was extremely useful to detection of alteration involving of AML1 and TEL genes. We demonstrate that interphase FISH is available to detect more prognostic important genetic abnormalities than conventional cytogenetic. Cytogenetic analysis combined with FISH produced significant improvements in the sensitivity and accuracy in identification of the of risk stratification of patients.
KEYWORDS acute lymphoblastic leukemia, cytogenetics, FISH, TEL-AML, BCR-ABL, MLL
Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None.
Competing interest / Conflict of interest:
The author(s) have no competing interests for financial support, publication of this research,patents, and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
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Original article
Jayanta Kumar Barua1, Mausumi Basu2,Susmita Bhattacharya3, Kheya Mukherjee4, Debasish Sinha5, Anita Nandi6
1Assistant Professor, Department of Dermatology, Venerology and Leprosy,The School of Tropical Medicine, Kolkata
2Associate Professor, Department of Community Medicine, IPGME&R and SSKM Hospital, Kolkata
3 Professor and 4Assistant Professor, Department of Microbiology, N.R.S. Medical College and Hospital, Kolkata
5 Assistant Professor, Department of Community Medicine, IPGME&R/ SSKM Hospital, Kolkata
6 Assistant Professor, Department of Microbiology,Medical College and Hospital, Kolkata
The name of the department(s) and institution(s) to which the work should be attributed: NRMC & H,Kolkata adjacent to attributed.
Address reprint requests to
*Dr. Mausumi Basu,
Associate Professor,Department of Community Medicine, IPGME&R and SSKM Hospital, Kolkata, India
Article citation: Barua JK, Basu M, Bhattacharya S, Mukherjee K, Sinha D, Nandi A. Epidemiological profile of HIV patients in a tertiary care hospital of Kolkata. J Pharm Biomed Sci 2015;05(11):890–896. Available at www.jpbms.info
Abstract:
Background Approximately 20.89 lakh people were living with HIV/AIDS (PLHA) in India in 2011 with prevalence rate of 0.27%. Objectives To assess the prevalence of HIV among consented attendees and to analyse the epidemiological profile of HIV-positive clients diagnosed in an ICTC of Kolkata. Materials and Methods A descriptive cross-sectional study was carried out throughthe analysis of secondary data from July 2012 to June 2013 in an ICTC centre attached with microbiology department of a medical college, Kolkata; using a pre designed proforma. Data were analysed using Epi Info version 3.5.1 and Statistical Package for Social Sciences (SPSS 22.0) software version. Results Counseling and testing services were provided to 14,679 clients; 158 were diagnosed as HIV positive with a prevalence of 1.07%; about 89.24% of seropositives belonged to age group of 15–49 years; males constituted 68.99%; 54.43% HIV were from rural area; 56.33% were married; literacy rate was 83.55%; 65.31% females were housewives; 39.8% of male clients undertook daily wage; 28.48% had sexual partner who were positive for HIV; 93.04% practiced high risk behaviour;78.48% acquired HIV through heterosexual route; 08.86% and 06.33% were involved in homosexual and bisexual practices respectively; and parent to child transmission rate was 5.70%. Conclusions This study provides an important clue to understand the epidemiology of HIV/ AIDS in a particular geographic region and to help an effective local planning for care, treatment and support of those infected and preventive strategies for those who are at risk.
KEYWORDS HIV positive clients, risk behaviour, ICTC, epidemiological profile
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Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None.
Competing interest / Conflict of interest:
The author(s) have no competing interests for financial support, publication of this research,patents, and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.