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Original article
MinSong1,Mei-HuaGao1,2,Wei-Huan Huang1,Man-Mei Li1,Hua Li2,Yao-Lan Li1,Xiao-Qi Zhang1*,Wen-Cai Ye1
1 Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China
2 Guangdong Institute for Food and Drug Control, Guangzhou 510180, China
Address reprint requests to:
*Xiao-Qi Zhang,
College of Pharmacy, Jinan University, No. 601 West Huangpu Avenue, Guangzhou 510632, China
Article citation: MinSong, Mei-HuaGao, Huang WH, Li MM, Li H, Li YL, et al.Flavonoids from the seeds of Hovenia acerba and their in vitro antiviral activity. J Pharm Biomed Sci 2016;06(06):401–409.Available at www.jpbms.info
ABSTRACT
A new flavonoid, 6″′-acetyl-spinosin (12), together with 14 known flavonoids, were isolated from Hovenia acerba. Their structures were elucidated by extensive spectroscopic methods. The rotamers of four flavonoid C-glycosides 12–15 was investigated for the first time. All the flavonoids were evaluated for their anti-RSV activity by cytopathic effect (CPE) reduction assay. As a result, five flavonoids, 3, 7, 10, 13, and 14, displayed better antiviral effect against three RSV strains. Plaque reduction assay demonstrated that compound 13 mainly inhibited the intracellular replication of RSV. HPLC analysis indicated that the active compounds 3, 7, and 13 had higher contents in the seeds.
KEYWORDS Hovenia acerba, Rhamnaceae, flavonoids, antiviral activity, RSV
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Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: This work was supported by grants from the National Natural Science Foundation (No. 81373935), the Science and Technology Planning Project of Guangdong Province (No. 2013A022100028), and the Administration of Traditional Chinese Medicine of Guangdong Province (No. 20131139).
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
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