DocumentsDate added
Original Research article:-
*S. Emmanuel Joshua Jebasingh1, S. Rosemary1, S. Elaiyaraja1, K. Sivaraman1, M. Lakshmikandan1, A. Murugan2, P. Raja2.
1.Department of Biotechnology, Sri Paramakalyani Center for Excellence in Environmental Science, Manonmaniam Sundaranar University, Tirunelveli, Tamil nadu, India- 627412. 2.Suganthi Devadason Marine Research Institute, Tuticorin, Tamil nadu, India- 628001.
Abstract:-The seaweed species (Enteromorpha compressa, Enteromorpha intestinalis, Caulerpa scalpelliformis, Caulerpa racemosa, Chaetomorpha linum, Gracilaria foliifera, Hypnea valentiae) were collected from Gulf of Mannar, Tuticorin coast and the samples were air dried: Six different solvents (hexane, diethyl ether, chloroform, ethyl acetate, ethanol, acetone and methanol) were used for the extraction of seaweeds. The antibacterial activity of seaweed extracts was checked against six human pathogens. The acetone extract of Caulerpa scalpelliformis showed broad spectrum antibacterial activity when compared to other seaweed extracts. Acetone extract of Caulerpa scalpelliformis was further fractionated and purified using chromatographic separation techniques using bioassay guided fractionation and the active fraction purity was checked by HPLC.
Keywords:-Seaweeds, Antibacterial activity, Gulf of Mannar, Caulerpa scalpelliformis, Natural product.
Original research article:-
*Veerendra K. Nanjwade1, F. V. Manvi1, Shamrez Ali. M2, Basavaraj K. Nanjwade1
1.Department of Pharmaceutics, KLE University College of Pharmacy, JN Medical College Campus, Belgaum – 590010, Karnataka, India.
2.Simpex Pharma Pvt. Ltd, Sigaadi, Kotdwara – 246149, Pauri Garhwal, Uttarakhand, India.
Abstract:-Pharmaceutical cocrystals are new solid forms with physicochemical properties that appear promising for drug product development.
Objective: In this study, we aimed to characterize the of Prulifloxacin-salicylic acid cocrystals by IR, DSC and PXRD in comparision with the pure drug.
Materials and Methods: Pharmaceutical cocrystals were prepared by the kneading method with aid of salicylic acid and urea as coformer. The cocrystals were subjected to various physicochemical studies and thermal methods.
Results and Discussion: The cocrystal of prulifloxacin , with salisylic acid (SA) has been shown to have higher solubility than prulifloxacin. In this study, we aimed to characterize the pure drug and the cocrystals with the salicylic acid and urea. Remarkably, two new cocrystals of prulifloxacin were discovered in this study. The study indicates that the improved aqueous solubility of the cocrystals leads to improved dissolution of Prulifloxacin. Thus, the cocrystals are a viable alternative solid form that can improve the dissolution rate and bioavailability of poorly soluble drugs.
Conclusion: Subsequently, differential scanning calorimetry was used to investigate the cocrystal formation. The formation of cocrystals was also verified using liquid-assisted grinding. The spectral patterns of prulifloxacin , salicylic acid and the complex were different.The physicochemical properties such as solubility and dissolution rate of this complex will be further investigated.
Key words: Pharmaceutical cocrystlas, Prulifloxacin, Salicylic acid, Complex.
Research article:-
Dhatri Devi. N1, *Siddalingeshwara K.Gurubasappa1, Sunil Dutt P L N S N2, Vishwanatha T3, and Ramesha I3, Jayaramu M4, Dwarakanath V4, Naveen M5 , Sumanth B.K6.
1.Department of Microbiology and Biochemistry Padmashree Institute of Information Sciences, Nagarabhavi, Circle.Bangalore-72, India.
2.Research and Development Centre Bharathiar University, Coimbatore, India. 3.Department of Microbiology and Zoology, Maharani Science college for Women, Bangalore-01.India.
4.Department of Microbiology and Biotechnology, Tumkur University, Tumkur, India. 5.Department of Microbiology, Govt Science College, Bangalore, India.
6.Department of Biotechnology, Kuvempu University. Shankaraghatta, India.
Abstract:- Twenty one Aspergillus wentii isolates were isolated from the soil samples were screened for L-glutaminase synthesis by plate assay method. Among the isolates the potential Aspergillus wentii SD4 were used for the synthesis in elevated level of L-glutaminase by optimizing the fermentation parameters like pH, temperature and inoculums size. The optimized pH will be 5.5 and it showed 1.71 IU. Temperature and inoculums sizes were also optimized as 350C and 1.0 ml (1x107). At this level these parameters shows 2.22IU and 2.73IU in elevated levels.
Key words: Aspergillus wentii, L-glutaminase, Fermentation kinetics, tumour inhibitor.
Original research article:-
Goel Chirag, Verma Pankaj, Ahmad Naseer, Nailwal K Tapan* Department of Biotechnology, Kumaun University, Nainital, Campus, Bhimtal - 263136, Uttarakhand, India.
Abstract:-Urtica parviflora is considered as an important Medicinal plant, due to its various ethanomedical uses. Here, we analyze the Genetic Variation in U.parviflora, with respect to plant distribution in Kumaun hills based on change in altitude. Examination of Random amplified Polymorphic DNA (RAPD) markers from four plant samples collected at different heights from sea level indicated that genetic variation was appreciable, as samples from lower altitudes showed low genetic similarity with samples collected from higher altitudes. A total of 70 scorable bands were produced in four samples with 8 primers. The average number of bands per primer was 8.75. Out of 70 bands, 48 bands were polymorphic (68.75%) noted in the present investigation. The dendrogram of the samples showed two major clusters. The samples of Mukteshwar, Nainital and Bhowali are in one cluster and Bhimtal in other cluster.
Key words: Genetic Diversity, Primers, RAPD analysis, Taq DNA polymerase.
Original research article:-
1Siddiqui Aslam Iqbal*,M.Pharm(Pharmaceutics),1Bakde Bharti Vidya,M.Pharm (Pharmaceutic), 3Dr. Kiran K Tappar (Ph.D.)
1.Pataldhamal Wadhwani College of pharmacy, SGB Amravati University, Yavatmal-445001, Maharashtra, India.
2.Vidya Bharati College of pharmacy, Amravati, Maharashtra, India.
Abstract:-The present research work aimed to design a gastro retentive floating drug delivery system for sustained release of Diltiazem Hydrochloride. Diltiazem HCl has site specific drug absorption from upper part of gastrointestinal tract and it is insoluble at higher pH (i.e. lower part of GI tract), therefore poorly absorbed from lower part of GI tract. Unabsorbed drug causes impaired therapeutic effect, increases frequency of dose administration and contributes to more side effects. Therefore a gastro retentive dosage form is required to ensure the controlled drug delivery of Diltiazem HCl within a drug absorbable region. Various grades of low density polymers (HPMC K4M, HPMC K15M, HPMC K100M and Xanthan gum) were used for formulation of this system. They were prepared by physical blending of Diltiazem HCl, polymers and effervescent agent in varying ratios. The formulation was optimized on the basis of In vitro bouncy and In vitro release in 0.1 N HCL buffer solutions. In vitro buoyancy was found to be in the range of 10 to 25 seconds and percent swelling index in the range of 130 to 332 %. Floating time was more than 12 hrs. In vitro drug release of the optimized batch (F5) was found to be 94.15 % at the end of 12th hr and it show the best fit model as matrix and it shows non-fickian type of drug release. The floating tablets were also evaluated for uniformity of weight, hardness, friability, drug content and effect of hardness on floating lag time.
Keywords: Effect of Hardness on Floating Lag Time and Total, Floating time, In vitro, Buoyancy studies, Mechanism of release, Water uptake study.