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Original research article:- Wong Man-Yi , BSc (Hons); Chiu Gigi N.C. , PhD
Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, Singapore 117543.
Abstract:- A reversed phase ultra performance liquid chromatography (UPLC) method was developed and validated to determine plasma levels of vincristine and quercetin simultaneously. Vincristine, quercetin and apigenin (internal standard) were extracted from plasma by liquid-liquid extraction with ethyl acetate. UPLC analysis was carried out at 25 ºC with a Waters AcQuity UPLC BEH C18 2.1 x 50 mm, 1.7 μm column with the gradient elution of water/formic acid (99.9:0.1, v/v) and acetonitrile/formic acid (99.9:0.1, v/v). The flow rate was 0.5 mL/min and the injection volume was 1 μL. Vincristine and quercetin were detected at wavelengths of 297 nm and 376 nm, respectively, with elution time of 1.45 and 1.13 minutes. The calibration curves were linear from 0.156 to 50 μg/mL for vincristine (r2=0.999) and 0.075 to 6.0 μg/mL (r2=1.000) for quercetin. The coefficients of variation (CV) for the intra- and inter-day assays were below 15.5%. The lower limit of quantitation for vincristine and quercetin in plasma were 0.1 μg/mL and 0.02 μg/mL, respectively. Absolute recoveries ranged from 103.2% to 104.8% for vincristine and 96.7% to 120.0% for quercetin. Both drugs were stable for 24 h at 4ºC. This method was successfully applied to determine plasma vincristine, quercetin metabolites and quercetin levels after intravenous administration in mice.
Key words :- Pharmacokinetics, quercetin, UPLC, vincristine.
Case Report:- C Bharath1, M.D, Shdakshari G2, M.D, DCP, Vijayanath.V3.
1.Professor, Department of Pathology, Govt. Medical College, VIMS ,Bellary – 583 104,Karnataka State, India.
2.Assistant Professor, Department of Pathology, Govt. Medical College, VIMS ,Bellary – 583 104,Karnataka State, India. 3 Associate Professor, Department of Forensic Medicine & Toxicology, S.S.Institute of Medical Sciences & Research centre, Davangere-577005,Karnataka, India.
Abstract:- Background :Granulomatous appendicitis is an extremely rare entity and its etiology is still unknown. It is a diagnosis by exclusion. Method:Appendix specimen was received in our department histopathology laboratory, processing was done by taking sections like horizontal and one transverse section from the tip of the appendix. Then processed in histokinette, sections of 4 – 5 microns were taken by microtome and stained with routine hematoxylin and eosin. Results : Microscopically showed non-caseating granulomas in the wall of the appendix, stain for AFB was negative and was reported as Granulomatous Appendicitis. Conclusion:Isolated Granulomatous inflammation of the appendix is very uncommon and rare entity. This should be carefully evaluated histologically as it may be misinterpreted as Crohn’s disease.
Key words :- Appendix, inflammation, granulomas, granulomatous appendicitis.
Research article:- *Dr. Uma Maheswara Rao V, M.Pharm, Ph.D1, Dr. Gaviraj E.N, M.Pharm, Ph.D, . 2 Veeresham C, M.Pharm, Ph.D,PDF,3
1.School of Pharmacy, Nalla Narasimha Reddy Educartion Society’s Group of Institutions, Korremula 'X' Road, Ghatke-sar (Mandal), Ranga Reddy (Dist), A.P, India- 500 088,India.
2.University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India. 3.University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India
Abstract:- The production of hypericins, which are antidepressant, anticancer and anti-HIV constituents of Hypericum perforartum (St. John’s Wort) by in vitro techniques is increasingly considered as an alternate source. Shoot cultures, at different growth phases, were therefore treated with a variety of precursors and organic compounds to stimulate the biosynthesis of hypericins. Since sodium acetate and emodin at 10 mg/l stimulated the accumulation of hypericins at a mid exponential growth phase, dose optimization of these precursors was carried out at the concentrations of 5, 10, 25 and 50 mg/l. A dose-dependent improvement in the yield was seen with sodium acetate. At 50 mg/l, the production of pseudohypericin (236.4 mg/l) increased by 2.4-folds while it was 2.2-folds high for hypericin (17.16 mg/l). However, emodin at 25 mg/l produced the greatest response. The accumulation of pseudohypericin increased by 3.2-fold to 309.7 mg/l and hypericin accumulated up to 25.8 mg/l, which was 2.5-folds more than that of the control. Prominent enhancement in the yields was achieved when sodium acetate and emodin were feeded simultaneously. The accumulation of pseudohypericin and hypericin was elevated, respectively, by 4.5- and 3.7-folds. Among the organic compounds, succinic acid (5 mM) and malic acid (10 mM) stimulated the production of hypericins at a mid exponential growth phase although the effect was moderate. Our study indicated a key role of sodium acetate and emodin in enhancing the accumulation of hypericins in shoot cultures of H.perforatum.
Key words :- Hypericum perforatum, hypericins, organic compounds, precursors, shoot cultures.
Original research article:- K. N. Pujari1, S. P. Jadkar1, Aruna Kulkarni2, C. G. Patil1, V. B. Tuljapurkar3
1.Assistant professor, Department of Biochemistry, Govt. Medical College, Miraj, India. 1Ph.D. student, Department of Biochemistry, Govt. Medical College, Miraj, India.
1.Lecturer in Statistics and Demography,Govt. Medical College, Miraj,India.
2.Ex Professor, Grant Medical College, Mumbai, Maharashtra,India.
3.Senior medical oncologist, Department of Medical Oncology, Shri Shiddhivinayak Ganapati Cancer Hospital, Miraj,India.
Abstract:-Catalase and glutathione peroxidase are the antioxidant enzymes produced naturally within the body. These help the body to convert hydrogen peroxide into water and oxygen, thus preventing the formation of carbon dioxide bubbles in the blood. The enzyme levels of catalase and glutathione peroxidase are altered to considerable extent in various diseased states exhibiting either elevation or depletion in their activity, this phenomenon was found to be more evident in leukemias. We have determined the catalase and glutathione peroxidase activity in the erythrocytes of leukemic patients and these levels were significantly low as compared to control. In respect to sex, the mean catalase levels in males (5.750 ± 0.310) were found to more than that in females (5.671 ± 0.301) however, this difference is non-significant. Whereas in respect to age, the mean catalase levels in age >11 years are decreased suddenly in AML patients (7.375± 0.050 to 5.730± 0.362). In case of glutathione peroxidase, we cannot found any change with respect to sex. However in age group <10 years and 11-20 years there is a sudden increase in mean glutathione peroxidase levels in AML patients (32.41 ± 0.635 to 33.28 ± 0..754). We found significant trend in catalase and GPx levels with respect to age in ALL only. We observed highly significant (p<0.001) catalase and glutathione peroxidase dismutase levels in acute leukemias with respect to age. Our results suggest that oxidative stress in leukemia patients causes the deficiency in antioxidant enzyme, which arise as a result of enormous production of reactive oxygen species in the system.
Key Words:-Leukemia,Catalase, Glutathione peroxidase, Hydrogen peroxide.
Research article:-*Tanuja S1, Dr. U. Dinesh Acharya2, *Shailesh K R3
1.Assistant Professor, Department of Computer Science Engineering, MIT, Manipal University, India.
2.Professor and Head, Department of Computer Science Engineering, MIT, Manipal University, India.
3.Assistant Professor (Sr.), Department of Electrical& Electronics,MIT, Manipal University,India. Abstract:-In this paper we present the performance analysis of different data mining techniques to predict the inpatient hospital length of stay in a super specialty hospital. Data set used for the analysis is real time data taken from super specialty hospital. Pre-processed data set is generated from the electronic discharge summaries obtained from the hospital. This data set consists of 401 records with 16 parameters. In this paper we have investigated four data mining techniques: Multilayer back propagation NN, Naive Bayes Classifier, K-NN method, J48 class of C4.5 decision tree. We found from the analysis that Neural Network has achieved better performance compared to the other three techniques.
Key words:- Data Mining, Text Mining, Back propagation, Neural Networks, K-NN, J48, Naive Bayes , Scoring System, missing data replacement.