DocumentsDate added
Research article:- Chaube Poonam H., Gandhi Santosh V. *, Deshpande Padmanabh B., Kulkarni Veena G. Department of Pharmaceutical Analysis, A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Pune - 411 001, MH, India.
Abstract:- A new simple, accurate, and precise densitometric method for determination of Paracetamol and Etodolac in spiked human plasma has been developed and validated. A Simple precipitation method was carried out by using methanol and a known amount of supernatant solution was spotted on precoated silica gel 60 F254 plates using a Camag Linomat V autosampler. Detection and quantitation were performed without using an internal standard. The mobile phase selected was Toluene: Ethyl acetate: Methanol (5:4:1, v/v/v) with UV detection at 234 nm. The retention factors for Paracetamol and Etodolac were found to be 0.40 ± 0.05 and 0.64 ± 0.05, respectively. The calibration curve was linear in the concentration range 100 to 600 ng/band for both the drugs in human plasma. The limit of quantitation was found to be 100 ng for both the drugs and no interference was found from endogenous compounds. The % recovery from human plasma using the precipitation method was found to be 93.06 for Paracetamol and 91.97 for Etodolac, respectively. The method provides a direct estimate of the amount of Paracetamol and Etodolac present in human plasma. The method was validated with respect to linearity, accuracy, precision and stability as per the Bioanlytical Method Validation guidelines.
Key Words:- Paracetamol, Etodolac, High Performance Thin Layer chromatography, human plasma.
Original research article:- M. Manikannan1, M.Sc., R. Balamurugan1, M.Sc., R. Varatharajan,2 S. Dinesh1, M.Sc., *Elanchezhiyan Manickan1, Ph.D., M.D.,
1. Research Scholars Division of Virology and Immunology, Department of Microbiology, Dr. ALM Post Graduate Institute of Basic Medical Science, University of Madras, Taramani, Chennai-600113, India.
1*Professor, Department of Microbiology, Dr.ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai- 600 113, India.
2.Medical officer, Voluntary Health Sciences, Adyar, Chennai- 600 020, India.
Abstract:- Background: Nitric oxide and cytokines are produced by the immune cells in response to various stimuli and the initial cytokine milieu at which immune cells interact have a positive impact on the outcome of the immune response generated. Over expression or polarization of a particular cytokine(s) is known for to affect the outcome of the disease. Immunotherapy is a novel approach to combat cytokine mediated diseases and several medicinal plants were shown to possess immune modulatory properties. One of the popular Indian medicinal plants known as Pongamia pinnata (P. pinnata) has been extensively studied for its bioactive properties however, immune modulatory property of the plant extract has not been widely studied. Hence the objective of this study is to study the immune stimulation property of P. pinnata on healthy human PBMCs. Methods: Extracts derived from various solvents were tested for their immune stimulation properties. Briefly, Nitric oxide (NO) production was analyzed upon stimulation of RAW 264.7 cells with plant extract and controls and the supernatants was collected and tested for NO. Similarly culture supernatants obtained at different time points from PBMC stimulated with the extract and were screened for Th-1 and Th-2 cytokines by ELISA. Results: Experiment conducted on RAW 264.7 cells indicated that P. pinnata extract stimulated NO activity. Of the solvents used, NO activity was best seen with aqueous extract. Secondly P. pinnata has induced remarkable production of IL-10. Other cytokines were not stimulated by this extract. Conclusion: Results of our study clearly indicated that P. pinnata extract induced higher levels of Nitric oxide. Maximum NO activity was noticed with aqueous extracts followed by the other extract preparations. It is known that NO can induce Th-2 cytokine and results are in support of this notion.
Key Words:- ELISA, Immune modulation, Immunotherapy, Nitric oxide, PBMC, Pongamia pinnata, Th-1, Th-2 cytokine.
Original research article:- Madaan Reecha*1(M. Pharm.), Bansal Gundeep2 (M. Pharm.), Sharma Anupam3 (Ph.D.)
1.Department of Pharmacy, Faculty of Pharmaceutical Sciences, NIMS University, Shobha Nagar, Jaipur Rajasthan, India.
2.Deparment of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147 002, Punjab, India
3.Pharmacognosy Section, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160 014, India .
Abstract:- Actaea spicata Linn. (Ranunculaceae) has been traditionally used for the treatment of various ailments such as rheumatism, inflammation, nervous disorders, lumbago, scrofula and chorea, but no work has ever been carried out for standardizing this potential plant. The present investigation establishes histological characters, micrometric determinations and physicochemical parameters for A. spicata. Microscopically, root of A. spicata showed the presence of lenticels, two layers of cork cells, 5-6 layers of cortex made up of parenchyma cells containing scattered calcium oxalate crystals, pericycle layer, medullary rays, xylem vessels and phloem parenchyma. Yellowish brown cork, parenchyma cells, scattered rosette shaped calcium oxalate crystals, lignified pitted vessels, pericyclic fibres, and starch grains simple as well as compound (2-7 cells) were observed in powdered microscopy of A. spicata roots. Total ash of A. spicata roots was about 3 and 5 times more than acid insoluble and water soluble ash respectively. Water soluble extractive value of the plant was found to be approximately seven times more than petroleum ether soluble extractive value and slightly higher than alcohol soluble extractive value. Thin layer chromatography of petroleum ether extract showed six spots using hexane : chloroform :: 17 : 3 as mobile phase whereas chloroform extract showed nine spots using toluene : ethyl acetate : glacial acetic acid :: 85 : 15 : 01 as mobile phase on spraying 0.5 % anisaldehyde followed by heating for 2 minutes at 105C. Phytochemically, the plant was found to contain alkaloids, flavonoids, steroids, tannins, proteins and carbohydrates.
Key words:- Actaea spicata, Alkaloids, Ash values, Extractive values, Flavonoids .
Case Report:- C Bharath1, M.D, Shdakshari G2, M.D, DCP, Vijayanath.V3.
1.Professor, Department of Pathology, Govt. Medical College, VIMS ,Bellary – 583 104,Karnataka State, India.
2.Assistant Professor, Department of Pathology, Govt. Medical College, VIMS ,Bellary – 583 104,Karnataka State, India. 3 Associate Professor, Department of Forensic Medicine & Toxicology, S.S.Institute of Medical Sciences & Research centre, Davangere-577005,Karnataka, India.
Abstract:- Background :Granulomatous appendicitis is an extremely rare entity and its etiology is still unknown. It is a diagnosis by exclusion. Method:Appendix specimen was received in our department histopathology laboratory, processing was done by taking sections like horizontal and one transverse section from the tip of the appendix. Then processed in histokinette, sections of 4 – 5 microns were taken by microtome and stained with routine hematoxylin and eosin. Results : Microscopically showed non-caseating granulomas in the wall of the appendix, stain for AFB was negative and was reported as Granulomatous Appendicitis. Conclusion:Isolated Granulomatous inflammation of the appendix is very uncommon and rare entity. This should be carefully evaluated histologically as it may be misinterpreted as Crohn’s disease.
Key words :- Appendix, inflammation, granulomas, granulomatous appendicitis.
Review article:- 1Altaf Hussain, 2Syed Abdul Aziz, 1Khan Usmanghani, 1M. Akram, 1H. M. Asif
1.Shifa ul Mulk Memorial Hospital, Faculty of Eastern Medicine, Hamdard University, Karachi, Pakistan.
2.Hamdard Institute of Education and Social Sciences, Hamdard University, Karachi, Pakistan.
Abstract:-Medicine as it stands today is being affected by new trends i.e. along with allopathic medicine; the emergence and resurgence of traditional medicine are appearing globally for health care. Therefore, it is imperative that policy and plans for traditional medicine should be chalked out so as to cover all the salient features of applications and implication to cure and prevent diseases. It has been pointed out what must be done appropriately to redirect the traditional medicine in the health scenario in charting the agenda. In order to cover the frame work a delineation has been made to high light the salient features of traditional medicine with respect to education, regulation, organization, practice and its implication in hospital, industry, agriculture and trade.
Key Words:- Traditional medicine, education and practice of traditional medicine, implication of traditional medicine.