DocumentsDate added
Review article:- *Gunjan Mahendru1, P. K. Sharma1, V. K. Garg1, A. K. Singh1, S. C. Mondal
1. Department of Pharmaceutical Technology, Meerut Institute of Engineering and Technology, Baghpat Bypass, NH-58, Meerut-250005, Uttar Pradesh, India.
Abstract:- Dengue has become a major health problem in India. It is mainly transmitted to humans by Aedes aegypti mosquito, which constitutes the etiological agents of the disease. So, for treating this disease goat milk and milk products are mostly preferred. Selenium (Se) is the main component of goat milk. Deficiency of Selenium and decrease in platelet count are the main complications of dengue fever. Goat milk as well as milk products are richest source of Selenium (Se) as comparison to cow and sheep milk. Goat milk also found to help with the digestive and metabolic utilization of various minerals.
Keywords:-Dengue fever, Goat Milk, Milk Products, Selenium, Platelet Count.
Research article:- *Punna Venkateshwarlu1, Srikanth Gajam2
1.Department of Quality Assurance, Nalanda College of Pharmacy, Cherlapally, Nalgonda, A.P, India.
2.Department of Pharmaceutics, Nalanda College of Pharmacy, Cherlapally, Nalgonda, A.P,India.
Abstract-:A simple, specific, accurate, and precise reverse phase liquid chromatographic method(RP-HPLC) was developed and validated for the estimation of Rizatriptan from bulk drugs. A RP : Inertsil ODS 3V (4.6mm x 250mm), 5µm in isocratic mode, with mobile phase containing 8 ml of Phosphate buffer, 58 ml of Acetonitrile and 34 ml of Methanol. The ratio pH was found to be 5.5. The flow rate was 1.0 ml/min and effluents were monitored at 225 nm. The retention time of Rizatriptan was 8.7 min. The linearity of the method was good (r > 0.998), as also were intra-day and inter-day precision (RSD <2%). The method was validated for accuracy, specificity, limit of quantification, limit of detection, robustness and stability. The results showed that proposed method is successfully applied for the quantitative determination of Rizatriptan in bulk drugs.
Key Words:- Reverse phase liquid chromatography, Rizatriptan, HPLC, specificity, validation.
Original research article:- *Kundan Singh Bora1 (M. Pharm.) and Anupam Sharma2 (Ph.D., M.D.)
1.NIMS University Rajasthan, Shobha Nagar, Jaipur-303 121, India.
2.University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160 014, India.
Abstract:- Medicago sativa Linn. (family: Leguminosae) has a long tradition of use as Ayurvedic and Homoeopathic medicine in variety of central nervous system (CNS) disorders. Traditionally, M. sativa is used to improve the memory, to cure kidney pain, cough, as a rejuvenator, antidiabetic, antioxidant, anti-inflammatory, antimicrobial, and in CNS disorders. Despite a long tradition of use, no systematic phytochemical and pharmacological work has been carried out on this potential plant. The present study has been designed to evaluate the anxiolytic activity of different fractions of bioactive methanol extract of the aerial parts of M. sativa using widely accepted elevated plus-maze model of anxiety in mice. An attempt has been made to isolate the bioactive fraction by resorting to bioactivity directed fractionation and column chromatographic technique. Sub-fraction F4 of bioactive ethyl acetate fraction which was derived from the methanol extract of the plant showed significant anxiolytic activity at a dose of 20 mg/kg, p.o., as compared to the standard drug, diazepam (2 mg/kg p.o.). TLC profile of this sub-fraction comprises of four spots indicating presence of flavonoid components. The study validates the traditional use of M. sativa for the treatment of CNS disorders like anxiety.
Key Words:- Anti-anxiety, Ethyl acetate fraction, Flavonoids, Medicago sativa.
Original research article:- *Pradhan Kishanta Kumar1, Mishra Uma Shankar1, Pattnaik Subasini2, Panigrahi Ghanshyam1, Pasa Gourishyam1, Sahu Kanhu Charana1.
1.Department of Pharmaceutical Analysis and Quality Assurance, Royal college of Pharmacy and Health Sciences, Andhapasara Road, Berhampur, Odisha, India.
2.Department of Zoology, Berhampur University, Bhanja Bihar, Berhampur, India.
Abstract:- A simple method for the estimation for the estimation of Valsartan in bulk and pharmaceutical dosage forms has been developed. Methanol was chosen as the solvent system.The λmax was found to be 249nm and all absorbance values were carried out at 249nm.The responses were linear in the range of 5-100µg/ml.The regression equation of the calibration graph and correlation coefficient were found to be y = 0.028x - 0.001 and 0.999 respectively. The %RSD values for both intraday and interday precision were less than 1%. The recovery of the drug from the sample was ranged between 97.77% and 101.4%. The proposed method was validated for accuracy, precision, robustness, ruggedness,LOD and LOQ.Commercial tablets containing 40mg and 80mg of valsartan were analysed by the proposed method and the results were well within the claimed limits.Furthermore stability studies of Valsartan were carried out under acidic, alkaline, hydrolytic, thermolytic, oxidation, photolytic and UV degradation conitions as per SIAM (Stability Indicating Assay Methods).
Key Words:- Analytical method validation, Beer’s law, Forced degradation, UV- spectrophotometry, Valsartan.
Original Research article:-
*Jadhav Ravindra T1, Patil Pratibha R.2, Patil Payal H2
1.M.J.Biopharm Pvt. Ltd., L-7, M.I.D.C. indl. Area, Taloja, Raigad District, Maharashtra., India. 2.Department of Quality Assurance & Pharmaceutics,R.C.Patel Institute of Pharmaceutical Education & Research,Shirpur- 425405, Dhule District, Maharashtra., India.
Abstract:-The aim of present work was to develop a semisolid preparation (ointment, gel, cream) of Thiocolchicoside which is a cen-trally acting skeletal muscle relaxant for the effective treatment of muscle spasm. Thiocolchicoside has been claimed to posses GABA mimetic & glycinergic actions in other way can say that Thiocolchicoside is a gama-aminobutyric receptor agonist. It has recently been shown that thiocolchiocoside activity can be ascribed to its ability of interacting with strychnine sensitive glycine receptors & therefore that compounds endowed with the glycino-mimetic activity can be used in rheumatologic-orthopedic field for their muscle relaxant properties. It is widely prescribed for treatment of muscle spasm, cramps, musculoskeletal & neuromuscular disorder. Thiocolchicoside is available in market in the form of capsules & Injection. The major problem associated with Thiocolchicoside is its bioavailability which is very low i.e. 25% only so in order to minimize drug loss due to first pass metabolism, and overcome problem associated with low bioavailability drug there is need to formulate semisolid preparation . The aim of the study was to formulate & evaluate thiocolchicoside ointment, gel & cream and comparative study of their drug release. In vitro release of thiocolchicoside from the three bases i.e., ointment, gel, cream to an aqueous receptor phase through whatman filter paper no.41 as well as mice skin was monitored spectrophotometrically at a wavelength of 259nm. In vitro release study results showed that the steady state fluxes of the drug from vehicles rank according to the following order: ointment >gel >cream. Ointment base showed considerably higher drug release than other vehicles.
Key words :- Thiocolchicoside, ointment, gel, cream, diffusion.