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Review article:- Munish Garg, *Monika Hooda, Jyoti Solanki, Sandeep Saini, Sneha Das.
Department of Pharmaceutical Sciences, Maharshi Dayanand University Rohtak, 124001, India.
Abstract:- Adulteration of herbal supplements with undeclared synthetic drugs or by mixing the analogues of prescription can cause a significant risk to public hAealth. Consumers of such adulterated products are at risk of developing serious adverse reactions, potentially leading to pulmonary hypertension, moderate aortic regurgitation, a prominent right heart failure, hypokalemia and even death. These analogues are not declared on the labeling and often created by replacing or adding functional groups to the original chemical. Thus, herbal manufacturers have made it more difficult for the analysts to detect these undeclared pharmaceutical analogues into their products. Although DSHEA has given responsibility to the FDA to enforce guidelines for safety and claims, but as per the regulations, FDA can investigate a supplement only after a safety problem has been reported in a particular formulation thus giving the herbal manufacturers enough courage to launch their unscrupulous products into the market. Therefore, a current need arises to check these practices for the proper quality control of these herbal formulations. In this regard, the enforcement of strict manufacturing guidelines, approval process and quality control conditions may well be a step forward towards the safer use of industrially produced herbal products. Moreover, quality and efficacy of medicinal products should be assessed by randomised clinical trials (RCT) before licenses can be issued.
Key words:- Herbal formulations, quality control of herbals, synthetic adulterants in herbals.
Original research article:- *Dr. S. Swaminathan1, Dr. M. S. Seshadri2, Dr. A. S. Kanagasabapathy3
1.Sr. Consultant & Head, Biochemistry Department Apollo Speciality Hospital, Chennai -600 035, India.
2.Prof & Head, Endocrinology, Christian Medical College, Vellore-632004, India.
3.Former Prof of Clinical Biochemistry Christian Medical College, Vellore -632004 ,India.
Abstract:- Recently there has been considerable interest in the use of hair as an index to the status of essential trace metal metabolism in the body particularly zinc. In many respects hair is ideal for such use. The sample can be readily obtained without pain or trauma for the patient, is easily collected and requires no special equipment or storage. The concentration of the metal in the hair is high and large quantities of samples are easily available, making the task of the analyst easier. Further, hair does not readily deteriorate, and can be stored until it is convenient to perform the analysis. In this study we have established a Quality Control (QC) method for the day to day measurement of zinc in human hair making use of a pool of human hair collected from both men and women. The QC thus prepared shows good stability for a period of six months. The intra and inter assay precision (%CV) using such a hair QC were 3.6 and 5.9 respectively and both values are within acceptable limits.
Keywords:-Quality Control, Hair zinc, SD, %CV, Atomic Absorption Spectrophotometer
Research article:-
* Jahir Alam Khan1, Parul Srivastava 2
1.R&D division, MRD LifeSciences (P) Ltd., Lucknow, India.
2.Amity Institute of Biotechnology, AMITY University, Lucknow, India.
Abstract:- Today bioremediation is considered as the most effective and pollution free method for removing crude oil pollution from contaminated sites, as this method makes use of microorganism. The study was designed to evaluate the capability of bacterial strains inhabiting oil contaminated sites to degrade unused engine oil. Four different bacterial species were isolated from oil contaminated sites in Lucknow. One of the isolate (MJP1103) showed maximum oil degradation potential in Minimal salt media supplemented with 1-5 % crude oil, the result showed increase in growth & protein concentration throughout incubation period for culture MJP1103.
Key words:- Crude oil, Coccus, Degradation, Bioremediation.
Research article:-
Arvind K. Srivastava*, Savita Srivastava, Swayam Prakash Srivastava, Deepak Raina, Rehan Ahmad, M.N.Srivastava, Ram Raghubir and Vijai Lakshmi.
Divisions of Biochemistry, Medicinal and Process Chemistry, Botany and Pharmacology CSIR-Central Drug Research Institute, Lucknow-226001,India.
Abstract: The standardized ethanolic extract of the epicarp of the fruits of a marine mangroove Xylocarpus granatum have been found antihyperglycaemic and antidyslipidemic in nature. The yield of the extract by percolation method at room temperature varies between 8 to 14 % depending upon the season of collection. Studies in normal as well as streptozotocin-induced diabetic rats showed that the extract is effective in the improvement oral glucose tolerance post sucrose load. The extract was also found efficient in declining blood glucose profile of STZ-induced diabetic rats and both glucose as well as cholesterol and triglycerides levels in low dosed streptozotocin- high fructose fed rats and dyslipidemic hamsters. In neonatlly STZ-treated and fructose fed rats, the extract completely controlled their abnormal oral glucose tolerance and improved insulin resistance. The altered biochemical markers i.e. glucose-6-phosphatase, phosphofructokinase, phosphoenolpyruvate carboxykinase, pyruvate kinase, lactate dehydrogenase and glycogen phosphorylase in liver and kidney and except glucose-6-phosphatase, all the other markers in muscles of fructose fed as well as neonatally STZ-treated were also found towards normalization after the oral treatment of CDR-134 D123 at 250 mg/kg dose for three weeks. Controlled clinical trials are going on with this preparation for its usage in the management of type 2 diabetes mellitus.
Key words :-Xylocarpus granatum, Streptozotocin-induced diabetic rats, Neonatally STZ-treated rats, Low dosed STZ treated- high fructose fed rats, Dyslipidemia, hyperglycaemia, hypertriglyceridemia, db/db mice, hamsters, antidiabetic activity, antidyslipidemic activity.
Original research article:- Dr.S.Swaminathan Ph.D1, Ms.Priya Gangadaran M.Sc, M.Phil1, Mr.T.Venkatesh2 Dr.Mitra Ghosh M.D3
1.Biochemistry department, Apollo Speciality Hospital, Chennai -35, India.
2.B.Tech , M.G.R University,Chennai -84, India.
3.Chief of Lab Services, Apollo Speciality Hospital, Chennai -35, India.
Abstract:- Copper is believed to be the switch that turns on the angiogenesis process in tumor cells, by activating the growth factor. Copper is necessary for fetal growth as well as in the production of hemoglobin . This study was undertaken on 40 female patients. in the age group of 16 to 62 years to establish the diagnostics utility of serum copper measurement along with the two tumor markers CEA and CA125. While copper measurement was done using a manual method, CEA and CA125 assays were carried out is fully automatic Roche diagnostic Elecsys 2010 which uses electrochemiluminescence technology. Appropriate quality controls were used to validate the accuracy of the results obtained. Excellent correlation (p<0.0001) between Cu and both the tumor markers were obtained thereby showing a very strong association between serum copper and the two tumor markers. This study concludes that measurement of serum copper along with the two tumor markers will be diagnostically useful in cancer screening / remission.
Key Words:- Copper, CEA, CA 125.