DocumentsDate added
Research article:-
P.V.Kamala Kumari*, G.Girija Sankar, T.Prabhakar
Pharmaceutical Biotechnology Division, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India.
Abstract:- Aim: Optimization of different process parameters for L-asparaginase production by Streptomyces griseoluteus WS3/1 and its validation using response surface methodology was carried out during the present study. Methods and results: Four medium factors were screened by conventional methods and subsequent optimization process using Central composite design (CCD) was performed to find out the optimium values of the selected parameters. Dextrose, L-asparagine, pH and inoculum level were found to be the best medium factors for the optimization of L-asparaginase production and central composite design experiments indicated the optimal values of dextrose, L-asparagine, pH, inoculum level were 0.45 % w/v, 1.162 % w/v, 7.4 and 10.36 % v/v respectively. Conclusions: The combination of conventional method with central composite design (CCD) was effective and reliable in selecting the statistically significant factors and determining the optimal levels of those factors in fermentation medium. This work demonstrates the usefulness of CCD for determining the optimum conditions for maximizing L-asparaginase production. Significance and impact of the study: The combined optimization method described here is the effective for screening medium factors as well as determining their optimum levels for the production of L-asparaginase by Streptomyces griseoluteus WS3/1.
Key words :-S.griseoluteus WS3/1, L-asparaginase, CCD.
Research article:-
*Dr.Kora.S.A1, Dr. Pramiladevi.R2
1,2Asso.Professor, Department of Medicine S.Nijalingappa Medical College and H.S.K.Hospital & Research Center, Bagalkot, Karnataka-India.
Abstract:-Introduction: Dialysis and transplantation have prolonged survival and quality of life in patients with chronic kidney disease (CKD). This is an exploratory/descriptive study, looking into the sociodemographic profile and social factors in these patients associated with acceptance of therapy. Association between attitudes and demographics were examined. Methods: A total of 178 patients who were admitted to department of Medicine in S.Nijalingappa Medical College and HSK Hospital & Research Center with CKD were prospectively evaluated during the years 2005-2011 and based on the degree of renal failure, were categorized into conservative therapy, dialysis alone, or transplantation. A detailed psycho-social assessment and counseling regarding the treatment options was done. Results and Discussion: The highest numbers of patients were from age group of 41 to 60 years. Of the study patients, 75.28% were males, 85.9% were married, 64.6% were self employed, 5.61and 94.39% were respectively from low and middle-income groups, 71.9% lived in nuclear families and 83.1% had an urban background. CKD Stage V patients were advised dialysis alone in 8.4% and dialysis and transplantation in 91.6%. The major psycho-social factors negatively influencing the acceptance of treatment were finance, logistics, no willing donors, no medically fit donors and/or lack of social support. Our study suggests that finance is one of the important factors deciding the acceptance of treatment. Social factors negatively affecting were logistics, lack of willing and medically fit donors and lack of social support.
Key words :- Chronic kidney disease (CKD), Dialysis, Renal replacement therapy, Socio-demographics.
Research article:-
R. Ramya, S. Prakash, S. Sudha*
Molecular Diagnosis and Drug Discovery Laboratory, Department of Biotechnology, School of Life Sciences, Karpagam University, Coimbatore, Tamilnadu, India.
Abstract:-Context: Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors. Its incidence is rated as one among the highest in the world and the use of tobacco in various forms is increasingly associated with the cause of OSCC. Tobacco contains many carcinogenic and mutagenic chemicals which induce the generation of free radicals and reactive oxygen species (ROS) with increased level of lipid peroxidation. Malondialdehyde (MDA) is a major lipid peroxidation product that is mutagenic and tumorigenic. Aim: To investigate the relationship between serum levels of malondialdehyde (MDA), a routinely used marker of oxidative stress, and squamous cell carcinoma of the oral cavity. Methods: Serum malondialdehyde (MDA) levels in 40 histologically proven OSCC cases and 40 age matched controls were analyzed. Results and conclusion: Serum MDA levels in cancer patients were significantly higher than those in controls (P < 0.05). There was a significant association between increased MDA levels and tobacco habits. The results of this study provide further evidence that there is relationship between serum MDA and OSCC. The study hypothesizes that oxidant/antioxidant imbalance may be one of the major factor responsible for the development of oral cancer.
Key words:- Oral squamous cell carcinoma, Malondialdehyde, Lipid peroxidation.
Research article:-
D. Ramya, Siddikuzzaman, Manjamalai A and V.M. Berlin Grace*
*Department of Biotechnology, Karunya University, Coimbatore-641 114, Tamil Nadu, India. Abstract:- Chemoprotection has emerged as a very effective therapeutic measure against carcinogenesis and during cancer.
All-trans retinoic acid (ATRA), an active metabolite of retinal, has been shown to exert anti-cancer activities in a number of types of cancer cells and tissues. In the present study, we have investigated the effect of ATRA on the physiologic changes normally observed in cancer patient during or after development of cancer. The parameters analysed are lung/liver tissue damage markers in serum, lipid profile in serum and the histologic changes in lung and liver during benzo(a)pyrene induced lung cancer development in BALB/c mice. Benzo(a)pyrene (50 mg/kg wt) induced lung cancer animals showed abnormal changes in the serum lipids, lipoproteins and marker enzymes such as gamma glutamyl transpeptidase and lactate dehydrogenase. An extensive lung/liver histologic change was also observed. Treatment with ATRA (0.585mg/kg body wt) remarkably reverted all the above alterations, which indicates the anticancer effect that was further confirmed by histopathological analysis. Overall, the above data shows that the anticancer effect of ATRA is more pronounced when used as an chemoprotective agent against B(a)P induced lung carcinogenesis.
Key words :- ATRA; Benzo(a)pyrene; Lipid profile; Tumor marker; Histopathology.
Research article:- *Dr. Mohammed M. Safhi
Neuroscience and Toxicology Unit, College of Pharmacy, Jazan University, Kingdom of Saudi Arabia.
Abstract:- Floating matrix tablets of propranolol hydrochloride were developed to prolong gastric residence time and leading to an increase in drug bioavailability. Floating tablets were prepared by the wet granulation technique by using polymers such as xanthan gum and guar gum with sodium bicarbonate as a gas-generating agent. Formulation F1 and F2 have drug: xanthan gum ratios 1:0.62 and 1:1.25, F3 and F4 have drug: guar gum ratios 1:6.2 and 1:1.23, F5 and F6 have 1:0.31 and 1:0.62. The floating tablets were evaluated for uniformity of weight, hardness, thickness, friability, drug content, in vitro buoyancy and dissolution studies. In all the formulations buoyancy time ranges from 45 to 134 sec and lag time ranges from 12-24 hrs. The formulation F6 shows the floating buoyancy time 134 sec, floating lag time 24 hours and drug release 94.36%. Non-fickian diffusion was confirmed as the drug release mechanism from these tablets indicated that water diffusion and polymer rearrangement played an essential role in drug release. The formulations give the best results in terms of floating duration, prolonged drug release, and improved in vitro bioavailability. The mechanism of drug release was diffusion controlled and followed zero- order kinetics.
Key words:- Propranolol hydrochloride, xanthan gum, guar gum, in vitro buoyancy and non-fickian diffusion.