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Research article:- *Gaurav M. Doshi 1, Gayatri.V.Aggarwal1, Pradnya Palekar Shanbhag1, Keyur. V. Shastri1 , Ojas Kumar Agrawal1, Abhijeet B. Bhalerao2, Sandhya K. Desai2.
1.VES College of Pharmacy, Hashu Advani Memorial Complex, Collectors Colony, Chembur (East), Mumbai – 400074. India.
2.Principal K. M. Kundnani College of Pharmacy, Plot no. 23, Jote Joy Bldg. Rambhau Salgaonkar Marg, CuffeParade, Colaba, Mumbai 400 005. India. Abstract:- Antioxidant properties Cassia auriculata flowers, leaves and roots by hydroxyl radical scavenging activity and improved N, N-Dimethyl-p phenylenediamine methods were studied. The methanolic extract of the plant parts were evaluated by two improved in-vitro antioxidant models. In the present study out of the plant parts undertaken for the studies especially flowers showed prominent amount of activity in comparison to leaves and roots thereby suggesting that the probable antioxidant activity which could be attributed due to the content of flavonoids noticed in preliminary phytochemical evaluation proving the utility of the flowers in enormous healing mechanisms.
Key words :-Medicinal Herb, Avaram, DPMD, Free Radicals, inflammation.
Research article:-
D. Ramya, Siddikuzzaman, Manjamalai A and V.M. Berlin Grace*
*Department of Biotechnology, Karunya University, Coimbatore-641 114, Tamil Nadu, India. Abstract:- Chemoprotection has emerged as a very effective therapeutic measure against carcinogenesis and during cancer.
All-trans retinoic acid (ATRA), an active metabolite of retinal, has been shown to exert anti-cancer activities in a number of types of cancer cells and tissues. In the present study, we have investigated the effect of ATRA on the physiologic changes normally observed in cancer patient during or after development of cancer. The parameters analysed are lung/liver tissue damage markers in serum, lipid profile in serum and the histologic changes in lung and liver during benzo(a)pyrene induced lung cancer development in BALB/c mice. Benzo(a)pyrene (50 mg/kg wt) induced lung cancer animals showed abnormal changes in the serum lipids, lipoproteins and marker enzymes such as gamma glutamyl transpeptidase and lactate dehydrogenase. An extensive lung/liver histologic change was also observed. Treatment with ATRA (0.585mg/kg body wt) remarkably reverted all the above alterations, which indicates the anticancer effect that was further confirmed by histopathological analysis. Overall, the above data shows that the anticancer effect of ATRA is more pronounced when used as an chemoprotective agent against B(a)P induced lung carcinogenesis.
Key words :- ATRA; Benzo(a)pyrene; Lipid profile; Tumor marker; Histopathology.
Research article:-
*Kishore Reddy A 1, P.Prathyusha2, Krishna Reddy P 1, Naresh Kumar V 1, N.Siva Kali Prasad1
1.Department of Pharmaceutics, Centre of Pharmaceutical Sciences ,Institute of Science and Technology ,JNTUH, Hyderabad ,Andhraprdesh ,India.
2.Department of Pharmaceutics, Sree Dattha Institute of Pharmacy, Hyderabad,India.
Abstract:-The aim of this study was to develop a floating microspheres of Anti microbial drug Cephalexin by using statistical optimization technique. Microspheres were prepared by using spray drying technique. Sodium alginate and PVA were used as polymers. A Taguchi Orthogonal Array design was selected to study the effect of formulation and process parameters in the development of microspheres. The independent variables studied are percentage of drug (X1), D:P ratio (X2), P:P ratio (X3), drying inlet temperature (X4) and pump feed rate (X5). The response variables evaluated are %yield (Y1), %EE (Y2) and microspheres size (Y3). Optimization was performed by using desirability function and based on the results of optimization one optimized formula was obtained. To validate the model, the optimized formula was formulated and subjected to in-vitro characterization. Percentage yield, drug content and entrapment efficiency of optimized formula was found to be 23.21±1.21 %, 93.98 ± 1.12 %, 88.46 ± 1.33 % respectively. Drug release at the end of 8 hours of dissolution studies was found to be 89.08±0.45%. The expected values thus obtained are very close to the predicted values indicating validation of model prediction. Thus it can be concluded that Cephalexin loaded microspheres could be successfully formulated with optimized condition of spray dryer to obtain maximum percentage yield, entrapment efficiency, desired particle size and drug release. Based on the results of stability studies, it may be concluded that the so developed optimized formula was found to be stable for long period of time.
Keywords:- Cephalexin, Floating Microspheres, Poly Vinyl Alcohol, Sodium alginate, In-vitro study, Spray drying.
Research article:-
P.V.Kamala Kumari*, G.Girija Sankar, T.Prabhakar
Pharmaceutical Biotechnology Division, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India.
Abstract:- Aim: Optimization of different process parameters for L-asparaginase production by Streptomyces griseoluteus WS3/1 and its validation using response surface methodology was carried out during the present study. Methods and results: Four medium factors were screened by conventional methods and subsequent optimization process using Central composite design (CCD) was performed to find out the optimium values of the selected parameters. Dextrose, L-asparagine, pH and inoculum level were found to be the best medium factors for the optimization of L-asparaginase production and central composite design experiments indicated the optimal values of dextrose, L-asparagine, pH, inoculum level were 0.45 % w/v, 1.162 % w/v, 7.4 and 10.36 % v/v respectively. Conclusions: The combination of conventional method with central composite design (CCD) was effective and reliable in selecting the statistically significant factors and determining the optimal levels of those factors in fermentation medium. This work demonstrates the usefulness of CCD for determining the optimum conditions for maximizing L-asparaginase production. Significance and impact of the study: The combined optimization method described here is the effective for screening medium factors as well as determining their optimum levels for the production of L-asparaginase by Streptomyces griseoluteus WS3/1.
Key words :-S.griseoluteus WS3/1, L-asparaginase, CCD.
Research article:-
* OC Adekunle, OM Olatunji
*Department of Medical Microbiology & Parasitology Ladoke Akintola University of Technology Osogbo, Nigeria. P.O. Box 14066, U.I. Ibadan, Oyo State.
Abstract:-The study was carried out in Osogbo among hospitalized patients in order to determine the incidence of staphylococcus aureus and the antibiotic sensitivity patterns of organism. Specimen were isolated from the nasal cavities of hospitalized patients between ages 0 to 75years. One hundred and five samples were analyzed. Antibiotic susceptibility tests were performed. All the nasal swab samples were positive for staphylococcus species. The age group 16-31 years had highest carriage rates (35.2%). Forty six (46) were staphylococcus aureus while 59 were other staphycoccus species. All staphylococcus species were sensitive to pefloxacin. The isolation of staphycoccus species in all patients showed high endemicity of the organism in hospital environment. Also, pefloxacin can be used as drug of choice in the environment.
Key words :- Staphylococcus aureus, nasal cavity, hospitalized patients, antibiotic.