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Research article:- *Amit Pandey1, Rajesh Kumar2
1.R&D Division, MRD LifeSciences, Lucknow-226010, India.
2.Institute of Biosciences & Biotechnology, CSJM University, Kanpur -802024, India.
Abstract :-The antibacterial properties of “Lawsonia inermis” commonly known as “Henna or Mehandi” tested against bacterial pathogens (S. aureus, P. aeruginosa and E. coli). Agar diffusion susceptibility test revealed inhibition zone of Henna sample. The Henna leaves were exhibiting best result followed by fruits, bark root and stems. The solvents were used ethanol, methanol, ethyl acetate and hot water, compare to all, ethanolic extract and ethyl acetate extract were showing best result against one gram positive culture Staphylococcus aureus (MTCC 2940) and two gram negative cultures Pseudomonas aeruginosa (MTCC 2453) and E. coli (MTCC 739). The MIC value was determined by using broth dilution methods. Ethanolic extract of Henna was subjected to get the MIC against test organisms and it was found to be 1.45 mg/ml for E. coli and Pseudomonas aeruginosa.
Keywords:- Antibacterial properties, ethanolic and ethyl acetate plant extract, MIC, zone of inhibition.
Research article:- Harison Masih1, Ajay Kumar Singh1, Yashab Kumar1, Aviral Srivastava1, Ravi Kant Singh*2, Santosh Kumar Mishra2, Kumar Shivam2.
*1.Dept. of Microbiology & Fermentation Technology, Sam Higginbottom Institute of Agriculture Technology & Sciences, Allahabad, U.P., India.
2.Department of Biotechnology, IMS Engineering College, Ghaziabad, U.P., India.
Abstract:- Production of a family of lipopetide antibiotic by using mutated strain B. subtilis in submerged fermentation system was investigated. The mutated strain M40 showed a higher antagonistic activity than wild strain of B. subtillus against the plant pathogens Erwinia carotovora var. carotovora. The mutated strain was inoculated in three different liquid culture media CPG, CPM, CPMCa++ to study the level of production of inhibitory substances & CPMCa++ was found best culture medium against plant pathogens. The antagonistic strain M40 was more active than wild strain and obtained metabolite was thermostable, protease resistance and classified as producer of antibiotic of metabolite group.
Key words:- Lipopetide antibiotic, B. subtilis, Mutated strain, Erwinia carotovora var. carotovora, Plant pathogens