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Research article:- * Jeyam M.1, Sushma R.2, Sharanya M.3, Poornima V.3
*1 Corresponding author, Assistant Professor, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil nadu, India.
2M.Sc. student, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil nadu, India. 3Research Scholars, Biochematics lab, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil nadu, India.
Abstract:-The Hutchinson Gilford Progeria Syndrome is a premature aging disorder in children. Progeria is a cause of progerin accumulation due to mutation in protein lamin A which can be inhibited by blocking the prenylation process. The addition of a farnesyl or geranyl moiety to the CAAX motif of the protein in the post translation step prevents the formation of mutant lamin A. In the present study, the inhibitors were screened for blocking the prenylation process by docking nutraceuticals with the Farnesyl Pyrophosphate Synthase (FPPS) using Autodock. The compound Madecassoside from Centella asiatica showed the least binding energy (-16.11 Kcal/mol) and lower inhibitory constant (1.55 μM) forming 7 hydrogen bond interactions with the active site residues VAL254 (2), LYS257, TYR204 LYS200, ASP243 of FPPS target.
Keywords:-Autodock; Centella asiatica; Nutraceuticals; Progeria; LaminA.
Research article:-
*Dr. Narayan Shrihari MD (Microbiology)1, Dr. KumudiniT.S D.Bact (Microbiology) 2, Dr. Mariraj. J MD (Microbiology) 3, Dr. Krishna.S MD (Microbiology) 4
1Asst. Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
2 Tutor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
3 Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
4 Professor and Head, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
Abstract:- Background: The public health importance of intestinal parasitic infections cannot be denied because of their high prevalence and global distribution. It is an established fact that intestinal parasitic infections can lead to a number of adverse effects like anemia, reduced physical growth, mental retardation, abdominal colic, cholestasis, cholecystitis and pancreatitis. Aims: To assess the prevalence of intestinal parasites among patients attending our Hospital. Settings and Design: A retrospective study Material and Methods: A retrospective laboratory analysis of stool samples was carried out for intestinal parasite examination in a tertiary care Hospital, Bellary. The records were collected from Microbiology Laboratory for a period of two years (October 2009 to September 2011). Results: In our study the prevalence of intestinal parasitic infection is 24.78%. There are nine different parasites encountered. The most common parasites identified were Entamoeba histolytica 25(43.86%), Cryptosporidium parvum 17 (29.82%) and Giardia lamblia 6 (10.53%). The other parasites present were Taenia species, Strongyloides stercoralis, Ascaris lumbricoides and Ancylostoma duodenale. Conclusions: Protozoa are more common than helminthes. It is an important public health problem. It is necessary to develop effective prevention and control strategies including health education and environmental hygiene.
Keywords:- cholestasis, cholecystitis, pancreatitis, protozoa and helminthes.
Research article:- *Dr. Narayan Shrihari MD (Microbiology)1, Dr. KumudiniT.S D.Bact (Microbiology) 2, Dr. Mariraj. J MD (Microbiology) 3, Dr. Krishna.S MD (Microbiology) 4
1Asst. Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
2 Tutor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
3 Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
4 Professor and Head, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
Abstract:- Background: Diarrhoea is a common complication of infection with HIV leading to weight loss and cachexia. It occurs in almost 90% of the HIV patients. Cryptosporidium parvum is a major cause of diarrhoea in developing countries, mainly affecting children and HIV infected individuals with low CD4 counts. The infection is self limiting in immunocompetent hosts but can be severe and persist in the immunocompromised and malnourished individuals. Aims: To know the prevalence of Cryptosporidiosis in HIV patients attending our Hospital and detection of Cryptosporidium parvum oocysts by modified Ziehl-Neelsen staining, Giemsa staining and Sheather sugar flotation technique. Settings and Design: A retrospective study for a period of one year Material and Methods: Stool samples from 51HIV patients with diarrhoea and one HIV patient without diarrhoea were examined for oocysts by modified Ziehl-Neelsen(ZN) staining, Giemsa(GS) staining and Sheather sugar flotation (SSF) technique. Results: Of the 52 stool samples, modified ZN staining detected 14 (26.92%), GS staining12 (23.07%) and SSF technique 17 (32.69%) stool samples positive for oocysts of Cryptosporidium parvum. Conclusions: There is a high prevalence of cryptosporidiosis in HIV infected patients and simple method modified ZN staining can detect oocyst in stool sample.
Keywords:- Oocysts, Giemsa staining, Sheather sugar flotation, Cryptosporidium parvum.
Research article:-
*Dr. Narayan Shrihari MD (Microbiology)1, Dr. KumudiniT.S D.Bact (Microbiology) 2, Dr. Mariraj. J MD (Microbiology) 3, Dr. Krishna.S MD (Microbiology) 4
1Asst. Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India. 2 Tutor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
3 Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India. 4 Professor and Head, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
Abstract:- Introduction: Human Immunodeficiency Virus (HIV) infected patients have an increased risk of developing tuberculosis (TB) due to a loss of cell mediated immunity, along with a quantitative decline in circulating CD4 lymphocytes and tuberculosis occurs sooner than other opportunistic infections. The dual (HIV/TB) infection compared with CD4 matched cohort of TB uninfected HIV patients. We sought to test the hypothesis; TB additionally contributes to reduction in CD4 count in HIV/TB co-infected patients and leads to greater improvement in count following treatment as compared to CD4 matched TB uninfected individuals. Material and Methods: This is a retrospective cohort study. We studied the change in CD4 cell count in two groups of patients, those with CD4 cell count >200 cells/mm3 (Group 1) and < 200 cells/mm3 (Group 2) at presentation. In each group the change in CD4 cell count in dually infected patients following six months anti-tuberculosis therapy (ATT) and anti-retroviral therapy (ART) was compared to cohort of CD4 matched TB uninfected patients only on ART. Results: In group1 (156 cases) dually infected patients CD4 count improved from 250 to450 cells/mm3 and in TB uninfected (control) patients the change was from 260 to422 cells/mm3. In group 2 (180 cases) dually infected subjects count improved from 150 to355 cells/mm3, where as in TB uninfected (control) patients improvement was from 170 to330 cells/mm3. Conclusion: Greater improvement in CD4 count with ATT and ART in dually infected patients, it may suggests that TB additionally influences the reduction of CD4 count in HIV patients.
Keywords:-Anti-tuberculosis therapy, anti-retroviral therapy and CD4 cell lymphocyte of cryptosporidiosis in HIV infected patients and simple method modified ZN staining can detect oocyst in stool sample.
Research article:-
Ashok P. Salave and *P. Gopal Reddy.
Shri Dnyaneshwar Mahavidyalaya, Newasa, Dist-Ahmednagar,India. *P.G. Department of Botany, P.V.P. College, Pravaranagar,Ahmednagar, India.
Abstract:-An extensive field visits were arranged in Dongargan areas to document the traditional ethnobotanical information hidden among the local inhabitants has been gathered through verbal and informal interviews at their working places during 2007-2008 is presented here in their language. In this paper 24 species belonging to 24 genera from 17 families used by the local inhabitants at local level in their routine life has been reported here to fill up the gap of ethnobotanical knowledge in this area.
Keywords:- Ethnobotany, ethnoflora, Dongargan, Traditional uses.