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Research article:- * Jeyam M.1, Sushma R.2, Sharanya M.3, Poornima V.3
*1 Corresponding author, Assistant Professor, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil nadu, India.
2M.Sc. student, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil nadu, India. 3Research Scholars, Biochematics lab, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil nadu, India.
Abstract:-The Hutchinson Gilford Progeria Syndrome is a premature aging disorder in children. Progeria is a cause of progerin accumulation due to mutation in protein lamin A which can be inhibited by blocking the prenylation process. The addition of a farnesyl or geranyl moiety to the CAAX motif of the protein in the post translation step prevents the formation of mutant lamin A. In the present study, the inhibitors were screened for blocking the prenylation process by docking nutraceuticals with the Farnesyl Pyrophosphate Synthase (FPPS) using Autodock. The compound Madecassoside from Centella asiatica showed the least binding energy (-16.11 Kcal/mol) and lower inhibitory constant (1.55 μM) forming 7 hydrogen bond interactions with the active site residues VAL254 (2), LYS257, TYR204 LYS200, ASP243 of FPPS target.
Keywords:-Autodock; Centella asiatica; Nutraceuticals; Progeria; LaminA.
Research article:- S.Prathap1, Arun Maiya2 , Saraswathi3, Jayachandran Dare4
1 Assistant Professor, College of Physiotherapy, Saveetha University, Chennai, India.
2 Associate Dean & head, Department of Physiotherapy, Manipal University, Manipal, India. 3Head of department, Dept of Anatomy, Saveetha Medical College, Chennai, India.
4 Director, Biomedical Research Unit & Laboratory Animal Centre, Saveetha University, Chennai, India.
Abstract:- Peripheral neuropathies have been described in patients with primary and secondary diabetes of diverse causes suggesting a common etiologic mechanism based on chronic hyperglycemia. The extensive review of literature reveals that there is paucity of studies on scope of management for this particular complication, this urges to do a study on this problem with low level laser irradiation which is recognized worldwide for its tissue healing properties. The Effect of various dosages of low level Laser Therapy in Experimental Diabetic Neuropathy was evaluated with 42 Healthy adult male albino wistar rats. The animals were induced with Alloxan intraperitonealy and blood glucose status was examined with Glucometer. Diabetic neuropathy status was measured with EMG-NCV for MNCV. The rats were than divided into 7 groups and irradiated with laser dosages ranging from 3j/cm2 to 8j/cm2 and one group was kept as control. On analyzing pre and post MNCV values, dosages of 3-4j/ cm2 showed extremely significant p values 5-6j/cm2 showed satisfactorily significant results and 7-8j/cm2 and control groups did not show any significant effect. This MNCV results are important finding of the study that the calculation of correct dosage of laser is very important, like higher dosage can have photo bio-inhibitory effect.
Key Words:- Low Level Laser Irradiation, Diabetic Neuropathy, Motor Nerve Conduction Velocity
Research article:-
*Dr. Narayan Shrihari MD (Microbiology)1, Dr. KumudiniT.S D.Bact (Microbiology) 2, Dr. Mariraj. J MD (Microbiology) 3, Dr. Krishna.S MD (Microbiology) 4
1Asst. Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India. 2 Tutor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
3 Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India. 4 Professor and Head, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
Abstract:- Introduction: Human Immunodeficiency Virus (HIV) infected patients have an increased risk of developing tuberculosis (TB) due to a loss of cell mediated immunity, along with a quantitative decline in circulating CD4 lymphocytes and tuberculosis occurs sooner than other opportunistic infections. The dual (HIV/TB) infection compared with CD4 matched cohort of TB uninfected HIV patients. We sought to test the hypothesis; TB additionally contributes to reduction in CD4 count in HIV/TB co-infected patients and leads to greater improvement in count following treatment as compared to CD4 matched TB uninfected individuals. Material and Methods: This is a retrospective cohort study. We studied the change in CD4 cell count in two groups of patients, those with CD4 cell count >200 cells/mm3 (Group 1) and < 200 cells/mm3 (Group 2) at presentation. In each group the change in CD4 cell count in dually infected patients following six months anti-tuberculosis therapy (ATT) and anti-retroviral therapy (ART) was compared to cohort of CD4 matched TB uninfected patients only on ART. Results: In group1 (156 cases) dually infected patients CD4 count improved from 250 to450 cells/mm3 and in TB uninfected (control) patients the change was from 260 to422 cells/mm3. In group 2 (180 cases) dually infected subjects count improved from 150 to355 cells/mm3, where as in TB uninfected (control) patients improvement was from 170 to330 cells/mm3. Conclusion: Greater improvement in CD4 count with ATT and ART in dually infected patients, it may suggests that TB additionally influences the reduction of CD4 count in HIV patients.
Keywords:-Anti-tuberculosis therapy, anti-retroviral therapy and CD4 cell lymphocyte of cryptosporidiosis in HIV infected patients and simple method modified ZN staining can detect oocyst in stool sample.
Research article:- * C.Venkatramaniah¹, A.Mary Antony Praba².
¹ Assistant Professor, Department of Anatomy, Sri Lakshmi Narayana Institute of Basic Medical Sciences, Pondy, India.
² Assistant Professor, Department of Anatomy, Tagore Medical College, Chennai, India
Abstract:- The skull is the ossified, bony structure that encloses and protects the brain, internal extensions of sensory organs, and some facial structures. When a complete skeleton is available, the level of reliability in establishing sex, age, and race through anthropometrics is almost 100%. Pelvic bones alone offer a 95% reliability , while pelvic bones plus the skull - 98%. By adulthood all the features in the skull are prominent and so identification of sex of a person is easy with in 30 years age. The smoother the skull, the older the person. So it is bit difficult to identify the sex above 30. So it is difficult to identify the sex of the person by using these three prominences alone. But there are certain points on the median plane of the skull which are helpful in finding the sex of a person. Though a number of median points are available with skull, we have selected only 9 prominent points were taken for the study they are the- Vertex, Glabella, Nasion, Akanthion, Bregma, Lambda, Inion, Basion And Opisthion(3,14). All the points are measured from the vertex. The result shows a considerable variation between the mean values of male and female skulls. The female skull shows a relatively low value in average than the male skulls. So this can be utilized in differenciating the sex of a person.
Keywords:- Vertex, glabella, nasion, akanthion, bregma, lambda, inion, basion, opisthion.
Research article:- S.P.S. Bisht*, B.V.V. Ravi Kumar, B. Praveen and S. R. Mishra
Department of Biotechnology, Roland Institute of Pharmaceutical Sciences, Berhampur-760010, Orissa, India.
Abstract:- Cypermethrin is a widely used pesticide based on pyrethroids. It is amongst the most effective pyrethroid preparations. The major target site of cypermethrin is the sodium channel of the nerve membrane. The present investigation was aimed to study the hepatotoxic effect of Cypermethrin at different dosages by estimating ASAT and ALAT enzyme levels in serum and the effect of Cypermethrin on weight gain of poultry birds. Cypermethrin was mixed with edible refined oil to prepare the feeding solution in the concentrations of 25 and 50 mg/kg body weight and fed to 18 birds in three groups for seven consecutive days. Birds treated with 1ml of refined oil served as Control (Gr I), 0.5ml of Cypermethrin solution @ 25 mg/kg body weight (Gr II) and with 0.25 ml of Cypermethrin solution @ 50 mg/kg body weight (Gr III) .The Gr II and GrIII showed a rapid rise in serum ASAT and ALAT levels and gained weight after 5th week of age indicating the hepatotoxic effect of Cypermethrin.
Key Words:- Cypermethrin, ASAT, ALAT, Hepatotoxic.