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Research article:- *Amit Pandey1, Rashmi Mishra2
1*R&D Division, MRD LifeSciences, Lucknow-226010, India.
2DAV College, CSJM University, Kanpur-802024, India.
Abstract: The present study was designated to evaluate the antibacterial activities of Ethanolic, Methanolic, Ethyl acetate and Hot water extract from leaves, fruits and peels of Aegle marmelos. Compare to all parts, the fruits were showing best result. The antibacterial activities of the extracts against bacteria were tested by agar well diffusion assay and the MIC values were determined by broth dilution assay. The methanolic and hot water extracts showed least antibacterial activity as compared to ethanolic and ethyl acetate extracts. The MIC values were obtained 1.98 mg/ml in ethanolic and ethyl acetate extract of fruits against S. aureus and 11.90mg/ml in methanolic extract against P. aeruginosa. The antibacterial compound mainly found in Aegle marmelos were tannins, phlobatannins, saponins, terpenoids, alkaloids and poly phenols.
Keywords: Antibacterial properties, ethanolic and methanolic plant extract, MIC, zone of inhibition.
Research article:- *Amit Pandey1, Afsheen2, Firdous Ara2, Sudeep Kumar Tiwari2
1R&D Division, MRD LifeSciences, Lucknow-226010, India.
2 IIMT, Aligarh- 202001, India.
Abstract:- The antibiotic sensitivity tests were performed for the isolated cultures obtained from hospital waste and laundry water in Lucknow. During the study, out of 16 cultures, 3 bacterial isolates were identified with the help of Bergey’s manual. They included; Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus species. The 6 antibiotics were used Amoxicillin, Ampicillin, Tetracycline, Ofloxacin, Chloramphenicol, Ciprofloxacin at lower to higher conc. (10µg- 10mg). The best results obtained for Ofloxacin, Ampicillin, Chloramphenicol antibiotics (10µg-1mg). The resistance of the 3 bacterial isolates to the commonly used antibiotics revealed that for all antibiotics, all the cultures were showing resistance and against Ofloxacin, it was 100% and for Chloramphenicol, the resistance activity was measured 80%. The MIC and MBC were also performed for identified cultures.
Key words:- Antibiotic sensitivity test, Resistance, MIC and MBC.
Research article:- *Amit Pandey1, Shweta2
1R&D Division, MRD LifeSciences, Lucknow-226010, India.
2DAV College, CSJM University, Kanpur-802024, India.
Abstract:-The present study was designated to evaluate the antifungal activities of ethanolic, methanolic, ethyl acetate and hot water extract from leaves and fruits of Psidium guajava. Compare to all parts, the fruits were showing best result and the zone of inhibition was obtained 29 mm in methanolic extract of fruits against T. rubrum. The antifungal activities of the extracts against fungus were tested by using agar well diffusion assay and the MIC values were determined by broth dilution assay. The hot water extracts showed lower antifungal activity as compared to methanolic, ethanolic and ethyl acetate extracts. The least concentrations were obtained 1.98 mg/ml in methanolic extract of fruits and ethanolic extract of leaves against T. rubrum and also 0.33 mg/ml for ethyl acetate extract of fruits against T. rubrum. The antifungal compound mainly found in Psidium guajava were tannins, phlobatannins, saponins, terpenoids, alkaloids and poly phenols.
Keywords:- Antifungal properties, ethanolic, ethyl acetate and methanolic plant extract, MIC, zone of inhibition.
Research article:- *Amit Pandey1, Arti Kaushik2, Sudeep Kumar Tiwari2
1R&D Division, MRD LifeSciences, Lucknow-226010, India.
2 IIMT, Aligarh- 202001, India.
Abstract:- The present study was carried out to find out the antimicrobial activity of ethanolic, methanolic, ethyl acetate & hot water extract of lemon fruit parts like peels & seeds. Antimicrobial analysis was done by using agar well diffusion method against bacterial and fungal pathogens. Methanolic extract of lemon peels exhibited the maximum zone of inhibition against Pseudomonas aeruginosa whereas hot water extract of lemon peels exhibited least zone of inhibition. Ethanolic extract of lemon seeds showed maximum zone of inhibition against Pseudomonas aeruginosa whereas hot water extract showed least zone of inhibition. MIC value was determined by using micro broth dilution method. The least concentration was obtained 2.4 mg/ml for ethanolic and hot water extracts of lemon peels against S. aureus. The MBC value also determined and phytochemical analysis showed the presence of tannins, glycosides, reducing sugars and flavonoids.
Keywords:- Antimicrobial analysis, methanolic, ethanolic & hot water plant extract, MIC, MBC, zone of inhibition, phytochemical analysis.
Research article:- S.P.S. Bisht*, B.V.V. Ravi Kumar, B. Praveen and S. R. Mishra
Department of Biotechnology, Roland Institute of Pharmaceutical Sciences, Berhampur-760010, Orissa, India.
Abstract:- Cypermethrin is a widely used pesticide based on pyrethroids. It is amongst the most effective pyrethroid preparations. The major target site of cypermethrin is the sodium channel of the nerve membrane. The present investigation was aimed to study the hepatotoxic effect of Cypermethrin at different dosages by estimating ASAT and ALAT enzyme levels in serum and the effect of Cypermethrin on weight gain of poultry birds. Cypermethrin was mixed with edible refined oil to prepare the feeding solution in the concentrations of 25 and 50 mg/kg body weight and fed to 18 birds in three groups for seven consecutive days. Birds treated with 1ml of refined oil served as Control (Gr I), 0.5ml of Cypermethrin solution @ 25 mg/kg body weight (Gr II) and with 0.25 ml of Cypermethrin solution @ 50 mg/kg body weight (Gr III) .The Gr II and GrIII showed a rapid rise in serum ASAT and ALAT levels and gained weight after 5th week of age indicating the hepatotoxic effect of Cypermethrin.
Key Words:- Cypermethrin, ASAT, ALAT, Hepatotoxic.