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Research article:-
*Dr. Narayan Shrihari MD (Microbiology)1, Dr. KumudiniT.S D.Bact (Microbiology) 2, Dr. Mariraj. J MD (Microbiology) 3, Dr. Krishna.S MD (Microbiology) 4
1Asst. Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
2 Tutor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
3 Professor, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
4 Professor and Head, Department of Microbiology, Vijayanagar Institute of Medical Sciences (VIMS), Bellary-583104, India.
Abstract:- Background: The public health importance of intestinal parasitic infections cannot be denied because of their high prevalence and global distribution. It is an established fact that intestinal parasitic infections can lead to a number of adverse effects like anemia, reduced physical growth, mental retardation, abdominal colic, cholestasis, cholecystitis and pancreatitis. Aims: To assess the prevalence of intestinal parasites among patients attending our Hospital. Settings and Design: A retrospective study Material and Methods: A retrospective laboratory analysis of stool samples was carried out for intestinal parasite examination in a tertiary care Hospital, Bellary. The records were collected from Microbiology Laboratory for a period of two years (October 2009 to September 2011). Results: In our study the prevalence of intestinal parasitic infection is 24.78%. There are nine different parasites encountered. The most common parasites identified were Entamoeba histolytica 25(43.86%), Cryptosporidium parvum 17 (29.82%) and Giardia lamblia 6 (10.53%). The other parasites present were Taenia species, Strongyloides stercoralis, Ascaris lumbricoides and Ancylostoma duodenale. Conclusions: Protozoa are more common than helminthes. It is an important public health problem. It is necessary to develop effective prevention and control strategies including health education and environmental hygiene.
Keywords:- cholestasis, cholecystitis, pancreatitis, protozoa and helminthes.
Research article:-Vaiyapuri Anandh1, Ivor Peter D’Sa2 , Jagatheesan Alagesan3, Vandana J. Rathod4.
1 Vaiyapuri Anandh - Professor & Principal, Saveetha College of Physiotherapy, Saveetha University, Chennai, India.
2 Ivor Peter D’Sa - Professor & Head, Department of Medicine, KSHEMA, Nitte University, Mangalore, India.
3 Jagatheesan Alagesan - Associate Professor, KJ Pandya College of Physiotherapy, Sumandeep Vidyapeeth, Vadodara, India.
4Vandana J. Rathod - Lecturer, KJ Pandya College of Physiotherapy, Sumandeep Vidyapeeth, Vadodara, India.
Abstract: Background and Objective: HIV infection is one of the chronic illnesses with an uncertain natural disease history. Exercise is well accepted as an adjunct therapy in the management of HIV. This study is intended to ascertain the effects of Progressive Resistance exercise on cardiovascular fitness and quality of life. Study Design: Pilot study Setting: Sneha Sadan HIV Home, Kaikamba. Outcome Measures: 3 Minute Step Test and MOS - HIV Health Survey Method: Fifteen HIV infected individuals were given Progressive Resistance exercise for 45 minutes, three days per week for three months after obtaining informed consent. Cardiovascular fitness and quality of life were assessed at the beginning and after three months of exercises. Result: The Mean ± SD before and after intervention for 3 minute step test is 3.60 ± 0.63 and 4.33 ± 0.72, for MOS-HIV Health Survey is 54.53 ± 8.44 and 62.40 ± 7.28 with p value equal to 0.001 for both outcome measures. Conclusion: This study concludes that Progressive Resistance exercises are effective for improving cardiovascular fitness and quality of life in HIV infected individuals.
Key Words: HIV, Progressive Resistance Exercises, Cardiovascular Fitness, Quality Of Life
Research article:- *Amit Pandey1, Afsheen2, Firdous Ara2, Sudeep Kumar Tiwari2
1R&D Division, MRD LifeSciences, Lucknow-226010, India.
2 IIMT, Aligarh- 202001, India.
Abstract:- The antibiotic sensitivity tests were performed for the isolated cultures obtained from hospital waste and laundry water in Lucknow. During the study, out of 16 cultures, 3 bacterial isolates were identified with the help of Bergey’s manual. They included; Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus species. The 6 antibiotics were used Amoxicillin, Ampicillin, Tetracycline, Ofloxacin, Chloramphenicol, Ciprofloxacin at lower to higher conc. (10µg- 10mg). The best results obtained for Ofloxacin, Ampicillin, Chloramphenicol antibiotics (10µg-1mg). The resistance of the 3 bacterial isolates to the commonly used antibiotics revealed that for all antibiotics, all the cultures were showing resistance and against Ofloxacin, it was 100% and for Chloramphenicol, the resistance activity was measured 80%. The MIC and MBC were also performed for identified cultures.
Key words:- Antibiotic sensitivity test, Resistance, MIC and MBC.
Research article:-
*Shirode Abhay R, Khanvilkar Vineeta V, Shah Jignesh M, Chitnis Aditi P, Kotadia Bhargav V, Kadam Vilasrao J.
Department of Pharmaceutical Chemistry and Pharmaceutical Analysis,Bharati Vidyapeeth’s College of Pharmacy, Navi Mumbai, Sector -8, CBD, Belapur, Navi Mumbai-400 614.
Abstract: Bioanalysis plays a key role in drug discovery process. When medicinal chemists are in the lead optimization phase of new drug discovery it is needed to know if their latest NCEs pass various drug metabolism and pharmacokinetics (DMPK) screens that check for their suitability in terms of pharmacokinetics and drug metabolism (e.g., in vitro stability, p450 inhibition and absorption potential). Bioanalytical processes can significantly enhance the drug discovery and development process. Bioanalysis helps a formulation scientists and clinical research team for the study of Bioavailability (BA) and bioequivalence (BE) of a developed dosage form of a particular drug. The use of high performance liquid chromatography (HPLC) has been the state-of-the-art analytical tool for drug-discovery bioanalysis and BA-BE studies for many decades. Over the time, the HPLC systems have improved and coupled with mass spectrometry (MS) and evolved as LC-MS then it is modified as LC-MS/MS. HPTLC is also used for the analysis of biological samples. In recent years when UPLC became commercially available, this brought biggest change. While MS/MS systems have also improved over last two decades, the basic principle of using selected reaction monitoring (SRM) for the analytical detection of the analyte of interest has remained the same. Thus we have been well served by the HPLC–MS/MS paradigm as our pre-eminent tool of choice for assaying both in vitro and in vivo samples of various types. Bioanlytical method development is a very crucial step to quantitate drug from biological matrix. Method must be established and validated to demonstrate total recovery, accuracy, precision, and robustness to determine the amount of drug present in the given biological matrix. This review will explore the approaches, chromatographic methods, and extraction techniques which are used and reported by different researchers for estimation of drug from various biological samples. This will also give valuable inputs to design a protocol for validation of a developed bioanalytical method. We predict that this review will provide significant ‘value-added’ data to many analysts and will certainly shorten the timelines of referencing for method development task.
Keywords: High performance liquid chromatography, Bioanalysis, reference.
Research article:- * Jeyam M.1, Sushma R.2, Sharanya M.3, Poornima V.3
*1 Corresponding author, Assistant Professor, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil nadu, India.
2M.Sc. student, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil nadu, India. 3Research Scholars, Biochematics lab, Department of Bioinformatics, Bharathiar University, Coimbatore-46, Tamil nadu, India.
Abstract:-The Hutchinson Gilford Progeria Syndrome is a premature aging disorder in children. Progeria is a cause of progerin accumulation due to mutation in protein lamin A which can be inhibited by blocking the prenylation process. The addition of a farnesyl or geranyl moiety to the CAAX motif of the protein in the post translation step prevents the formation of mutant lamin A. In the present study, the inhibitors were screened for blocking the prenylation process by docking nutraceuticals with the Farnesyl Pyrophosphate Synthase (FPPS) using Autodock. The compound Madecassoside from Centella asiatica showed the least binding energy (-16.11 Kcal/mol) and lower inhibitory constant (1.55 μM) forming 7 hydrogen bond interactions with the active site residues VAL254 (2), LYS257, TYR204 LYS200, ASP243 of FPPS target.
Keywords:-Autodock; Centella asiatica; Nutraceuticals; Progeria; LaminA.