DocumentsDate added
Original research article:-
Samatha P1, *Siva Prabodh V2, Chowdary NVS2, Ravi Shekhar2
1MSC , 2 MD, Dept. of Biochemistry, NRI Medical College and Hospital, Chinakakani, A.P- 522503, India.
Abstract:- Diabetic patients with accompanied (but often unnoticed) dyslipidemia are soft targets of cardiovascular deaths. An early intervention to normalize circulating lipids shown to reduce cardiovascular complications and mortality. Glycated hemoglobin (HbA1c) is a routinely used marker for long – term glycemic control. This investigation is an attempt to evaluate the association between HbA1c and various lipid parameters. Venous blood samples collected from 50 type 2 diabetic patients (26 males, 24 females) and serum analyzed for HbA1c, Fasting blood glucose, Total Cholesterol, Triacylglycerols, HDL-C and LDL-C. L/H risk ratio is also calculated. The levels of HbA1c (%) did not differ significantly between males (8.51 ± 1.17) and females (8.24 ± 1.89), whereas male patients had higher mean values of FBG and various lipid parameters than in females. Patients with HbA1c value >7.0 % showed direct and significant correlation with FBG, TC, LDL-C, L/H risk ratio as compared to patients with HbA1c ≤ 7.0%. There was no significant difference in TG, HDL-C between two groups of glycated hemoglobin. These findings indicate that HbA1c is utilized for screening high risk diabetic patients for early diagnosis of dyslipidemia and timely intervention with lipid lowering drugs.
Keywords:-Diabetes, Dyslipidemia, Glycated hemoglobin, Association.
Research article:-
P.A. Subramanian1, Abraha Gebrekidan1, 2*, and Kebede Nigussie1, 3
1Department of Chemistry, College of Natural and Computational Sciences, Mekelle University, P.O.Box 231, Mekelle, Ethiopia.
2Department of Chemical Engineering, Laboratory of Applied Physical Chemistry and Environmental Technology, Katholieke Universiteit Leuven, Belgium.
3Department of Chemistry, Addis Ababa University, Addis Ababa, Ethiopia.
Abstract:- Eucalyptus trees are available abundantly in the Tigray Region, Ethiopia. The investigation has been carried out to find the feasibility for the extraction of medicinal quality eucalyptus oil in this area. A survey in this Region showed that Hagereselam, Korem and Maychew are the potential areas to collect the samples. The species has been identified as Eucalptus globulus in all the highlands of Tigray Region. Hydro-distillation method was employed for the extraction of oil. The oil extracted in the sample area has been analyzed to have 1.4602 - 1.4623 refractive index value, 0.918 - 0.919 specific gravity (sp.gr.), +9 - +10 optical rotation and negative for phellandrene test that satisfy the standards stipulated by British Pharmacopeia. The percentage oil content in the Eucalyptus globulus has been found to be in the range 0.8 - 2.0% w/w (% dry weight) depending upon the dryness of the leaves. The essential oils were analyzed by GC/MS where the composition of the ten commonly found compounds in the oils are as follows: 1,8-cineole (66.28 - 75.36%), cis-ocimen (15.92 - 21.33%), α-terpineol acetate (2.70 - 3.39%), α-terpineol (1.51 - 2.26%), aromadendrene (0.69 - 2.85%), globulol (0.82 - 1.43%), β-pinen (0.96 - 1.24%), β-myrcene (0.66 - 1.00%), 4-terpineol (0.46 - 0.52%) and camphene (0.16 - 0.27%) as the main leaf oil components. The oils could be used for medicinal purpose except in the case of Maychew it needs purification and enrichment so as to make its 1,8-cineole content greater than 70%.
Keywords:-Eucalyptus globules, Hydro-distillation, Eucalyptus oil, GC/MS, 1,8-Cineole,Tigray
Original research article:-
*Singh Umesh Pratap 1 and R. Parthasarathy.2
1 Ph.D research scholar- NIMS University Jaipur Rajasthan,India.
2 Director-Faculty of Pharmacy, Kamla Nehru Institute of Management and Technology, Sultanpur, Uttar Pradesh, India.
Abstract:- Roots of Blumea eriantha DC and Blumea lacera have been used in the ayurveda and other folk medicines for the treatment of cholera and diarrhea. To justify its folklore, present study was undertaken to investigate the antidiarrhoel activity of the ethanolic extract from the roots of Blumea eriantha DC (EEBE) and Blumea lacera (EEBL.) Preliminary phytochemical screening, acute toxicity study and antidiarrhoel activity were studied on castor oil induced diarrhea and normal gastrointestinal models of rats at 250, 500 and 750 mg/kg body weight. The preliminary phytochemical screening of the ethanolic extract from the roots of Blumea eriantha DC (EEBE) results with the presence of anthraquinone glycoside, tannins, flavonoids etc.while Blumea lacera showed presence of steroids, terpenoids and alkaloids, flavonoids, saponin and absence of tannin and phnolics, LD50> 1000mg/kg. The doses of EEBE and EEBL both significantly decreased (P<0.001) the total number of diarrheal faeces. PI decreases doses dependently (250, 500 and 750 mg/kg orally) of EEBE and EEBL and was comparable with standard drug atropine sulphate. Conclusively, EEBL has more antidiarrhoel activity in comparison of EEBE but less effective when compared with atropine sulphate in experimental rats.
Keywords:-Blumea eriantha DC (EEBE)., Loperamide, Atropine. Blumea lacera (EEBL)
Original article:-
*Rudrama Devi K, Kusumlatha C, Dilip Reddy K, J. Karuna Kumari and Minny Jael.
Human Genetics and Molecular Biology Laboratory, Department of Zoology, Osmania University, Hyderabad-7, India.
Abstract:- Background: A number of antineoplatic drugs are used to combat with different types of cancer, and had been shown to cytotoxic. Adriamycin is one of most commonly used in malignant lymphomas. The antioxidants such as VitC inhibits the toxicity of mutagens/carcinogens in animals and Humans. Aim: The protective effects of ascorbic acid in adriamycin induced toxicity in swiss male mice was evaluated using sperm morphology assay. Material and Methods: Two experiments were conducted in germ cells of Swiss male mice. The cauda epidymis was collected from control and exposed group of animals after five weeks of treatment schedule and screened for presence of various types of sperm head abnormalities such as amorphous, banana hammer head etc., Results: The animals treated with 5, 10 and 20 mg/kg of ascorbic acid showed as non mutagenic where as the adriamycin induced significant increase in the percentage sperm head abnormalities but when primed with AA there is decrease in the percentages of abnormal sperms. Conclusion: VitC protects the adriamycin induced genotoxicity in germ cells of mice. Hence VitC supplementation is safer in chemotherapeutic strategy.
Keywords:- Ascorbic acid, genotoxicity, adriamycin, sperm morphology.
Original research article:-
*Shilpa.K.Gokale1, S. C. Metgud2.
1Asst. Prof, Dept of Microbiology, SN Medical College, Bagalkot.587102, India.
2Professor, Dept of Microbiology, JNMC, Belgaum, India.
Abstract:- Introduction: -Nonfermenters are being isolated from various clinical specimens. Although frequently considered as contaminants, the pathogenic potential has been proved beyond doubt by their frequent isolation from clinical material and their association with disease. They exhibit resistance not only to the beta lactam and other group of antibiotics but also to carbapenems. Objectives: To characterize the nonfermenters isolated from various clinical samples. To assess their clinical significance. To know their anti microbial sensitivity pattern. Materials and methods: The present study was conducted for a period of one year from Jan 2007 – Dec 2007 at J.N Medical College, Belgaum. The nonfermenters were identified using a standard protocol, which included tests for motility, oxidase production, oxidation-fermentation (OF) test for various sugars, DNAse test and ONPG test. The clinical significance was assessed using various criteria and the susceptibility testing was done by Kirby bauer disc diffusion method. Results: From a total of 130 NFGNB, Pseudomonas aeruginosa was the commonest nonfermenter accounting for 82.3% followed by Acinetobacter baumanii (15.4%). Other significant NFGNB isolated were: Acinetobacter lwofii (0.76%), Burkholderia pseudomallei (0.76%) and Moraxella species (0.76%). Pseudomonas aeruginosa showed good sensitivity to meropenem (96.2%), ciprofloxacin (50%) and amikacin (49.5%). Acinetobacter baumanii showed 96.2% sensitivity to meropenem and 45% sensitivity to ciprofloxacin. Conclusion: P aeruginosa and A baumanii were the common NFGNB isolated in our study. They were associated with various health care related infections like urinary tract infection, blood stream infections, surgical site infections and ventilator associated pneumonia. P aeruginosa showed good sensitivity to meropenem, ciprofloxacin and amikacin. A.baumanii showed good sensitivity to meropenem and ciprofloxacin. The susceptibility pattern of NFGNB varied depending upon the species. Therefore it is essential to identify the nonfermenters to know their trends in sensitivity.
Keywords:-Nonfermenters, P. aeruginosa, A. baumanii, Meropenem, Ciprofloxacin.