DocumentsDate added
Research article:-
P.A. Subramanian1, Abraha Gebrekidan1, 2*, and Kebede Nigussie1, 3
1Department of Chemistry, College of Natural and Computational Sciences, Mekelle University, P.O.Box 231, Mekelle, Ethiopia.
2Department of Chemical Engineering, Laboratory of Applied Physical Chemistry and Environmental Technology, Katholieke Universiteit Leuven, Belgium.
3Department of Chemistry, Addis Ababa University, Addis Ababa, Ethiopia.
Abstract:- Eucalyptus trees are available abundantly in the Tigray Region, Ethiopia. The investigation has been carried out to find the feasibility for the extraction of medicinal quality eucalyptus oil in this area. A survey in this Region showed that Hagereselam, Korem and Maychew are the potential areas to collect the samples. The species has been identified as Eucalptus globulus in all the highlands of Tigray Region. Hydro-distillation method was employed for the extraction of oil. The oil extracted in the sample area has been analyzed to have 1.4602 - 1.4623 refractive index value, 0.918 - 0.919 specific gravity (sp.gr.), +9 - +10 optical rotation and negative for phellandrene test that satisfy the standards stipulated by British Pharmacopeia. The percentage oil content in the Eucalyptus globulus has been found to be in the range 0.8 - 2.0% w/w (% dry weight) depending upon the dryness of the leaves. The essential oils were analyzed by GC/MS where the composition of the ten commonly found compounds in the oils are as follows: 1,8-cineole (66.28 - 75.36%), cis-ocimen (15.92 - 21.33%), α-terpineol acetate (2.70 - 3.39%), α-terpineol (1.51 - 2.26%), aromadendrene (0.69 - 2.85%), globulol (0.82 - 1.43%), β-pinen (0.96 - 1.24%), β-myrcene (0.66 - 1.00%), 4-terpineol (0.46 - 0.52%) and camphene (0.16 - 0.27%) as the main leaf oil components. The oils could be used for medicinal purpose except in the case of Maychew it needs purification and enrichment so as to make its 1,8-cineole content greater than 70%.
Keywords:-Eucalyptus globules, Hydro-distillation, Eucalyptus oil, GC/MS, 1,8-Cineole,Tigray
Original article:-
Jaspreet Kaur1, K.M.Prabhu2, L.C.Thakur3.
1Professor,H.O.D.,I.T.S. Centre for Dental Studies and Research, Delhi-Meerut Road, Murad Nagar, Ghaziabad- 201206,India.
2Professor , Department of Biochemistry, 3 Professor , Department of neurology ,UCMS & GTB Hospital, Shahdra, New Delhi, India.
Abstract:- Serum magnesium is an indispensable element for man .It is indispensable activator of over 300 enzymes in humans. Hypomagnesia (ionized form) leads to neuromuscular hyperirritability, tremors, increased vascular resistance ,coronary vasospasm and hypertension . Magnesium deficiency triggers vasoconstriction, enhances vascular endothelial injury and hence leads to atherosclerosis.The present study evaluated serum magnesium levels in transient ischaemic attack and stroke patients. Method: The study was carried on 50 diagnosed cases of acute ischaemic stroke,10 cases of TIA and 60 healthy controls(age and sex matched) Fasting blood samples were collected and assessed for serum magnesium levels. Statistical analysis was performed P value less than 0.05 were considered significant. Result: The value of serum magnesium is significantly lower in patient when compared with controls. Conclusion: Magnesium levels are significantly decreased in ischaemic stroke, though hemorrhagic stroke patients as well as patients of transient ischaemic attack also had magnesium deficiency. Magnesium deficiency predisposes atherosclerosis, the known modifiable risk factor of stroke.
Keywords:- Atherosclerosis, Diabetes mellitus, magnesium, transient ischemic attack, stroke.
Original article:-
Bhusari Vidhya K. 1, Dhaneshwar Sunil R. 2*
1Ph.D. Scholar, Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune, Maharashtra, India 411038.
2Professor & Chairperson, Department of Pharm.Chemistry,RAK Medical & Health Sciences University, College of Pharmaceutical Sciences, Ras Al Khaimah Post Box No 11172,U.A.E.
Abstract:- HPLC method has been described for simultaneous determination of Amlodipine besylate, Atenolol and Aspirin in formulation. This method is based on HPLC separation of the three drugs on the Thermo Hypersil BDS–C18 (250 mm × 4.6 mm, 5.0 µ) from Germany with isocratic conditions and simple mobile phase containing methanol: 10 mM phosphate buffer with pH 7.0 adjusted with ortho phosphoric acid (70: 30) at flow rate of 1 mL/min using UV detection at 235 nm with Rt of 2.58 min for Amlodipine besylate, Rt of 3.40 min for Atenolol and 4.23 min for Aspirin. This method has been applied to formulation without interference of excipients of formulation. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 2-12 µg/mL for Amlodipine besylate, Atenolol and 4-24 µg/mL for Aspirin, respectively. The mean values of the correlation coefficient, slope and intercept were 0.9993 ± 0.63, 2134.1 ± 0.54 and 1676 ± 0.89 for Amlodipine besylate, 0.9994 ± 0.91, 21326 ± 1.02 and 42960 ± 0.74 for Atenolol and 0.9993 ± 1.02, 15182 ± 0.48 and 64910 ± 0.64 for Aspirin, respectively. The method was validated for precision, robustness and recovery. The limit of detection (LOD) and limit of quantitation (LOQ) was 0.5 μg/mL and 1 μg/mL for Amlodipine besylate and Atenolol and 1 μg/mL and 2 μg/mL for Aspirin, respectively. Statistical analysis showed that the method is repeatable and selective for the estimation of Amlodipine besylate, Atenolol and Aspirin.
Key Words:-Amlodipine besylate, Aspirin, Atenolol, HPLC, Method development, Validation.
Original research article:-
Makwava Prakash V 1, Odedara Ram V 2, *Shah Harsh D 3
1MD Medicine, associate professor, Head of the unit,Department of medicine, 2MD Medicine, 3 Resident, MD , Department of community medicine , Guru Govind Singh Hospital, MP Shah Medical College, Jamnagar, Gujarat , India.
Abstract:- The wide spread use of organophosphorus compounds has increased incidence of its poisoning to the human kind by accidental or suicidal. Organophosphate (OP) insecticides inhibit both acetyl cholinesterase and pseudocholinesterase activities. This study helps to focus to make management protocols during morbidity at the time of presentation of poisoning. Method: A cross sectional descriptive study was done on fifty patients, by simple random sampling technique who came to emergency ward of Hospital, Jamnagar. After setting inclusion and exclusion criteria, Pre tested profoma was filled up. Analysis was done with primary information, clinical admission POP score, pseudo cholinesterase, hospital stay, atropinization requirements, need of ventilator support and mortality. Intravenous atropine and pralidoxime was administered as soon as possible. The diagnosis was performed from the history taken from the patient or the patient’s relatives after taking consent. Results: Amongst fifty patients, 78% were male and 22% were females. Mean age of the studied patients was 28 ± 10 years. In the study, inhalational poisoning (54%) had common occurrences than ingestion poisoning (46%) but mortality (6%) was found higher with ingestion. The compound that was exposed most commonly is monochrotophos (60%). Association of POP score came significant at p < 0.05 with above mentions factors. Conclusion: OP insecticide poisoning is a serious condition that requires rapid diagnosis and treatment. The patients with evidence of moderate and severe degrees of poisoning need close monitoring, as respiratory failure is the prime cause. Early management protocol will help to reduce mortality of poisoning.
Keywords:-Organophosphorus, poisoning.
Original research article:-
*Singh Umesh Pratap 1 and R. Parthasarathy.2
1 Ph.D research scholar- NIMS University Jaipur Rajasthan,India.
2 Director-Faculty of Pharmacy, Kamla Nehru Institute of Management and Technology, Sultanpur, Uttar Pradesh, India.
Abstract:- Roots of Blumea eriantha DC and Blumea lacera have been used in the ayurveda and other folk medicines for the treatment of cholera and diarrhea. To justify its folklore, present study was undertaken to investigate the antidiarrhoel activity of the ethanolic extract from the roots of Blumea eriantha DC (EEBE) and Blumea lacera (EEBL.) Preliminary phytochemical screening, acute toxicity study and antidiarrhoel activity were studied on castor oil induced diarrhea and normal gastrointestinal models of rats at 250, 500 and 750 mg/kg body weight. The preliminary phytochemical screening of the ethanolic extract from the roots of Blumea eriantha DC (EEBE) results with the presence of anthraquinone glycoside, tannins, flavonoids etc.while Blumea lacera showed presence of steroids, terpenoids and alkaloids, flavonoids, saponin and absence of tannin and phnolics, LD50> 1000mg/kg. The doses of EEBE and EEBL both significantly decreased (P<0.001) the total number of diarrheal faeces. PI decreases doses dependently (250, 500 and 750 mg/kg orally) of EEBE and EEBL and was comparable with standard drug atropine sulphate. Conclusively, EEBL has more antidiarrhoel activity in comparison of EEBE but less effective when compared with atropine sulphate in experimental rats.
Keywords:-Blumea eriantha DC (EEBE)., Loperamide, Atropine. Blumea lacera (EEBL)