DocumentsDate added
Original article:-
Bhusari Vidhya K. 1, Dhaneshwar Sunil R. 2*
1Ph.D. Scholar, Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune, Maharashtra, India 411038.
2Professor & Chairperson, Department of Pharm.Chemistry,RAK Medical & Health Sciences University, College of Pharmaceutical Sciences, Ras Al Khaimah Post Box No 11172,U.A.E.
Abstract:- HPLC method has been described for simultaneous determination of Amlodipine besylate, Atenolol and Aspirin in formulation. This method is based on HPLC separation of the three drugs on the Thermo Hypersil BDS–C18 (250 mm × 4.6 mm, 5.0 µ) from Germany with isocratic conditions and simple mobile phase containing methanol: 10 mM phosphate buffer with pH 7.0 adjusted with ortho phosphoric acid (70: 30) at flow rate of 1 mL/min using UV detection at 235 nm with Rt of 2.58 min for Amlodipine besylate, Rt of 3.40 min for Atenolol and 4.23 min for Aspirin. This method has been applied to formulation without interference of excipients of formulation. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 2-12 µg/mL for Amlodipine besylate, Atenolol and 4-24 µg/mL for Aspirin, respectively. The mean values of the correlation coefficient, slope and intercept were 0.9993 ± 0.63, 2134.1 ± 0.54 and 1676 ± 0.89 for Amlodipine besylate, 0.9994 ± 0.91, 21326 ± 1.02 and 42960 ± 0.74 for Atenolol and 0.9993 ± 1.02, 15182 ± 0.48 and 64910 ± 0.64 for Aspirin, respectively. The method was validated for precision, robustness and recovery. The limit of detection (LOD) and limit of quantitation (LOQ) was 0.5 μg/mL and 1 μg/mL for Amlodipine besylate and Atenolol and 1 μg/mL and 2 μg/mL for Aspirin, respectively. Statistical analysis showed that the method is repeatable and selective for the estimation of Amlodipine besylate, Atenolol and Aspirin.
Key Words:-Amlodipine besylate, Aspirin, Atenolol, HPLC, Method development, Validation.
Research article:-
*Kabnurkar R. B. *
Kabnurkar R. B., M.Pharm. Ph.D. Head, Dept.of Pharmacy P.V.Polytechnic, S.N.D.T.(W)University,Mumbai-400049,India.
Abstract:- Prepared,topical gel formulations containing active methanolic fraction of the fresh oil, of the seeds of Celastrus paniculatus Willd (Celastraceae), using Carbomer - 940, propylene glycol and glycerin. Studied the formulations, with their best compositions for physicochemical, rheological properties and stability over a period of one year. Developed a sensitive spectrophotometric method for the estimation of the active fraction ,at λ max =278 nm. Linearity studies, along with the reproducibility of the analytical method and its freedom from interference of excipients at λ max were found. Evaluation of the selected gel formulations indicated that the average content of the methanolic extract was well within ± 5%w/w limits of the labeled amount and their topical administration induced a dose dependent reduction in inflammation on carrageenan induced rat paw oedema.
Keywords:-Anti–inflammatory,Celastrus paniculatus Willd, Gel formulations.
Original research article:- Dental Sciences
*Rizwan M Sanadi
Reader, Dept of Periodontics, Yerala Medical Trust & Research Centre’s ,Dental College & Hospital, PG Institution, Institutional Area, Sector – 4, Kharghar, Navi Mumbai- 410210 ,Maharashtra, India.
Abstract:- Aggressive periodontitis comprises a group of rare, severe, rapidly progressing forms of periodontitis characterized by an early age of clinical manifestation and a distinctive tendency for cases to aggregate in families. Aggressive periodontitis, typically results in severe, rapid destruction of the tooth supporting apparatus. The increased susceptibility of the host population with aggressive periodontitis may be caused by the combined effects of multiple genes and their interaction with various environmental factors. Co-enzyme Q10 (Co-Q10) is essentially a vitamin or vitamin-like substance present naturally in foods and every cell of the body. Co-Q10 appears to be helpful in gingivitis. Recent studies indicate a significant improvement in all the signs and symptoms of periodontal disease. Hence the present study was conducted to test the clinical efficacy of this substance incorporated in the tooth and gum gel (Co-Q Dent) in aggressive periodontitis patients.
Keywords:-Aggressive Periodontitis, Co-enzyme Q10, bleeding gums, periodontal pocket, gingival inflammation
Original research article:-
*Shilpa.K.Gokale1, S. C. Metgud2.
1Asst. Prof, Dept of Microbiology, SN Medical College, Bagalkot.587102, India.
2Professor, Dept of Microbiology, JNMC, Belgaum, India.
Abstract:- Introduction: -Nonfermenters are being isolated from various clinical specimens. Although frequently considered as contaminants, the pathogenic potential has been proved beyond doubt by their frequent isolation from clinical material and their association with disease. They exhibit resistance not only to the beta lactam and other group of antibiotics but also to carbapenems. Objectives: To characterize the nonfermenters isolated from various clinical samples. To assess their clinical significance. To know their anti microbial sensitivity pattern. Materials and methods: The present study was conducted for a period of one year from Jan 2007 – Dec 2007 at J.N Medical College, Belgaum. The nonfermenters were identified using a standard protocol, which included tests for motility, oxidase production, oxidation-fermentation (OF) test for various sugars, DNAse test and ONPG test. The clinical significance was assessed using various criteria and the susceptibility testing was done by Kirby bauer disc diffusion method. Results: From a total of 130 NFGNB, Pseudomonas aeruginosa was the commonest nonfermenter accounting for 82.3% followed by Acinetobacter baumanii (15.4%). Other significant NFGNB isolated were: Acinetobacter lwofii (0.76%), Burkholderia pseudomallei (0.76%) and Moraxella species (0.76%). Pseudomonas aeruginosa showed good sensitivity to meropenem (96.2%), ciprofloxacin (50%) and amikacin (49.5%). Acinetobacter baumanii showed 96.2% sensitivity to meropenem and 45% sensitivity to ciprofloxacin. Conclusion: P aeruginosa and A baumanii were the common NFGNB isolated in our study. They were associated with various health care related infections like urinary tract infection, blood stream infections, surgical site infections and ventilator associated pneumonia. P aeruginosa showed good sensitivity to meropenem, ciprofloxacin and amikacin. A.baumanii showed good sensitivity to meropenem and ciprofloxacin. The susceptibility pattern of NFGNB varied depending upon the species. Therefore it is essential to identify the nonfermenters to know their trends in sensitivity.
Keywords:-Nonfermenters, P. aeruginosa, A. baumanii, Meropenem, Ciprofloxacin.
Original article:-
*Rudrama Devi K, Kusumlatha C, Dilip Reddy K, J. Karuna Kumari and Minny Jael.
Human Genetics and Molecular Biology Laboratory, Department of Zoology, Osmania University, Hyderabad-7, India.
Abstract:- Background: A number of antineoplatic drugs are used to combat with different types of cancer, and had been shown to cytotoxic. Adriamycin is one of most commonly used in malignant lymphomas. The antioxidants such as VitC inhibits the toxicity of mutagens/carcinogens in animals and Humans. Aim: The protective effects of ascorbic acid in adriamycin induced toxicity in swiss male mice was evaluated using sperm morphology assay. Material and Methods: Two experiments were conducted in germ cells of Swiss male mice. The cauda epidymis was collected from control and exposed group of animals after five weeks of treatment schedule and screened for presence of various types of sperm head abnormalities such as amorphous, banana hammer head etc., Results: The animals treated with 5, 10 and 20 mg/kg of ascorbic acid showed as non mutagenic where as the adriamycin induced significant increase in the percentage sperm head abnormalities but when primed with AA there is decrease in the percentages of abnormal sperms. Conclusion: VitC protects the adriamycin induced genotoxicity in germ cells of mice. Hence VitC supplementation is safer in chemotherapeutic strategy.
Keywords:- Ascorbic acid, genotoxicity, adriamycin, sperm morphology.