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Research article:- Biotechnology
* Verma Alpika ,Feroz Nadia, Verma Vipul, Rastogi Meenal, Shukla Neha.
Amity Institute of Biotechnology, Amity University, Lucknow, India.
Abstract:- Bacteria are present everywhere in the environment..The aim of the present study is to isolate the air borne bacteria & to check their inhibition by natural plant extracts of Lemon leaves(Citrus limon), Tea leaves(Camelia sinensis) and Ashoka leaves(Saraca asoca). Air borne bacteria were isolated from the Amity University Lucknow campus & were also characterized. Antimicrobial activity was checked using well diffusion method. Plant extracts were prepared from Acetone, Propanol, Methanol & Water. Methanol extract [1] of Saraca asoca was found to be most active against gram positive, anaerobic bacteria giving zone of inhibition of 20mm. It was also found that water extract of Citrus limon was most effective against gram negative, endospore producing, anaerobic bacteria with zone of inhibition of 17mm.
Keywords:- Citrus limon ,Camelia sinensis ,Saraca asoca, well diffusion, zone of inhibition, airborne bacteria.
Original research article:-
* Oluwamodupe Emmanuel Giwa1* and Tesleem A. Ibrahim2
1Department of Science Laboratory Technology, Rufus Giwa Polytechnic P.M.B. 1019 Owo, Ondo State, Nigeria.
2Department of Food Science Technology, Rufus Giwa Polytechnic P.M.B. 1019 Owo, Ondo State, Nigeria.
Abstract:- Wheat flour was used to substitute mushroom flour at the ratio of 70:30, 50:50, 30:70 and with spices (Alllium sativum) which concentration range from 5g, 10g, and 15g respectively. The cookies prepared without wheat flour and mushroom flour serves as positive control. The spread ratios were determined with a meter role. Total viable count, Staphylococcus, Bacillus and colliform count were determine using standard microbiological method and the effect of spices (Alllium sativum) concentration was measured. Consumer preference or otherwise are also determine using a taste panel list. The main quality score of microbial count of the cookies ranges at, 70:30 (1.0x103, 8.0x102 and 6.0x102 cfu for total viable count) (1.5x103, 1.2.x103 and 9.0x102 cfu for fungal count) (1.6x103, 1.2x103 and 1.0x103 cfu for Bacillus count) (1.7x103, 1.6x103, and 1.4x103 cfu for Staphylococcus count) (no count for colliform). At the range of 50:50 (1.3x103, 1.0x103 and 8.0x102 cfu for total viable count) (1.3x103, 1.0x103 and 8.0x102 cfu for Bacillus) (1.85x103,1.65x103 and 1.50x103 cfu for Staphylococcus count). At the range of 30:70 (2.0x103, 1.5x103 and 1.0x103 cfu for total viable) (1.1x103, 9.0x102 and 7.0x102 cfu for fungi count) (1.85x103, 1.50x103, and 1.20x103 cfu for Bacillus count) ( 2.0x103, 1.6x103 and 1.0x103 cfu for Staphylococcus count). The physical quality parameter indicates a range of spread ratio 70:30; 1.283x103, 1.266x103 and 1.250x103, 50:50, (1.250x103, 1.216x103 and 1.183x103). As the spice concentration increases in the order of 5g, 10g and 15g respectively. The main quality sensory score of the cookies ranges of 70:30, colour (5.0, 4.5 and 4.0), flavour (5.0, 4.5 and 4.0), taste (4.5, 4.0 and 3.5), overall acceptability (5.0, 4.0 and 3.5). The range of 50:50, colour (4.5, 4.0 and 3.5), flavour, (4.5, 4.0 and 4.0), taste (3.5, 3.0 and 3.0), overall acceptability (4.0, 3.5 and 3.5).At the range of 30:70, colour (4.0. 3.0 and 2.5), flavour (3.0, 3.0 and 3.0), taste (3.0, 3.0 and 3.0), overall acceptability (3.5, 3.0 and 3.0).
Keywords:- Alllium sativum, Colliform, Cookies, Termitomyces robustus, Wheat flour, composite flour.
Original research article:-
*Ekram M. Hassan1, M.S. Mahrous2, Ruba N. Shdeed2
1 Department of Pharmaceutical Analytical Chemistry and Quality Control.
2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy Beirut Arab University, Beirut,Lebanon.
Abstract:- Stability indicating, rapid and reliable derivative, absorption difference and derivative difference spectrophotometric methods were developed for the determination of ofloxacin and ceftriaxone in presence of their degradation products. The linear calibration graphs for ofloxacin were obtained from D1 at 281 nm for the acid solutions, D2 at 288.5 nm for the alkaline solutions, ΔA at 298.6 nm, ΔD1 at 292-304.2 nm, and ΔD2 at 298 nm, while those for its corresponding degradation product were obtained from D2 at 281.5 nm of the acid solutions, and D1 and D2 at 288.5 nm and 297 nm, respectively, for the alkaline solutions. On the other side, ceftriaxone was quantified using D2 amplitudes of the solutions in water at 241.3 nm, D1 at 268.3 nm and 238.5 nm, D2 at 257 nm and 283.4 nm, and D3 at 269.7 nm and 298.3 nm, for the acid solutions, while its corresponding degradation product was determined by measuring the D1 and D2 of the solutions in water at 241 nm and 249.8 nm, respectively, D1 and D3 of the acid solutions at 263 nm for both, D1 at 242.2 nm and 270.3 nm, and D2 at 248.7 nm of the alkaline solutions. The proposed methods were successfully applied to the determination of ofloxacin and ceftriaxone in synthetic mixtures with the corresponding degradation products. The results obtained indicated the high accuracy, calculated as mean percentage recovery, RSD% and Er% and precision; evaluated in terms of inter-day and intra-day precision. In addition, ofloxacin capsules and ceftriaxone ampoules were assayed using the proposed methods and the results obtained were compared with reference spectrophotometric methods.
Keywords: Ofloxacin, ceftriaxone, derivative, absorption difference, derivative.
original research article:-
*K. Ashok Augustine Raj1, K.Langeswaran2, P. Krishnamoorthy3.
1Assistant Professor in Bioinformatics, 2Assistant Professor in Industrial Biotech, 3Dean-Bioengineering Bharath University, Chennai, TN,India.
Abstract:- The effects of lycopene supplementation on PCB induced endocrine interference caused by PCBs in rat Sertoli cells of PCBs exposed adult rats was investigated. The severity of the endocrine disruption was determined by measuring Traits of reproductive organs; sperm characteristics, testicular histological findings, plasma testosterone levels and the testicular tissue oxidative status were determined. Induction of PCB to rats decreased sperm concentration (p<0.05) and sperm motility (p<0.001), increased total abnormal spermrates (p<0.05) as compared with the control group. An increase in testes malondialdehyde concentrations (p<0.05) and glutathione peroxidase activities (p<0.001) were detected while significant decreases in glutathione levels (p<0.001) in PCB alone group when compared to control group. While pre-treatment with lycopene restoring only malondialdehyde concentrations, its post-treatment caused normalization in both malondialdehyde and glutathione levels when compared with the PCB alone group. On the other hand, significant increases were determined in GSH-Px activities in all experimental groups when compared with the control group. In conclusion, lycopene supplementation was found to be able to diminish the severity of PCB induced endocrine disruption.
Research article:-
*M. Srujan Kumar1, Binayak Das1, S.V.S.Rama Raju2
1Department of Pharmaceutics, Samskruti college of Pharmacy,Kondapur,R.R District,Hyderabad,India.
2Department of Pharmaceutics, C.L.Baid Metha College of pharmacy, Chennai, India.
Abstract:- Compaction of multiparticulate, commonly called MUPS, is one of the more recent and challenging technologies that combine the advantages of both tablets and pellet-filled capsules in one dosage form. Venlafaxine is an anti depressant, having an elimination half-life of 5±2 hrs and its maximum daily dose is 300mg. Since the drug belongs to BCS class I, it is necessary to retard dissolution to ensure extended release of drug. The objective of the study is to prepare venlafaxine extended release pellets by extrusion spheronization technology, coating them with mixture of rate controlling polymers Ethyl cellulose and different grades of (Hydroxy propyl methyl cellulose (HPMC) using Wurster process to achieve the desired dissolution pattern and compressing the pellets into tablets. The pellets were analyzed for the parameters such as bulk density, tapped density, compressibility index, Hausner’s ratio and the results were found to be within the limits. Drug release rate was more when compared with the innovator sample. The Venlafaxine hydrochloride extended release pellets were compressed into the tablets. The dissolution profile of the prepared Venlafaxine hydrochloride extended release tablets were compared with that of Innovator (VENLAR). Finally conclude like extended release pellets in tablets of formulation (F9) have more drug release rate rather than innovator (VENLAR) and it have better Bioavailability.
Keywords:- Extrusion and Spheronization, HPMC, MUPS, multiparticulate, wurster process.