DocumentsDate added
Case study:-General surgery
*Satya Ranjan Patra1, V Srinivas2, GL Prasad3
1Associate Professor in General Surgery ,Madha Medical College And Hospital ,Thandalam Kovur Chennai 600122.India.
2Assistant Professor,3 Professor & HOD , General Surgery, PSI Medical College & Hospital ,Chinnaoutapalli Ganavaram, Andhra Pradesh 521286,India.
Abstract:- A case with recurrent small bowel obstruction, which was operated for a small bowel tumour four years ago, was operated upon and was found to be suffering with enteric stricture; enterocolic fistula and multiple mesenteric lymph nodes. Intestinal obstruction was managed by resection of the lesions and primary anastomosis of the intestine. Histo pathology revealed recurrent small bowel adenocarcinoma with lymphnode metastasis. Patient underwent 5- FU based chemotherapy and survived for twenty months.
Keywords:- Recurrent Small bowel adenocarcinoma, malignant obstruction, adjuvant chemotherapy.
Research article:-
Das S, Tiwari K L, Sen S, * Singh A. G D. Rungta College of Science and Technology, Kohka, Kurud road, Bhilai, (C.G.),India.
Abstract:-DNA extraction is an important step in molecular assays and plays a vital role in obtaining high resolution results in gel-based systems. So far Many genomic DNA extraction protocols have been described. Here a rapid DNA extraction method is described which is a very simple, fast, universally applicable and reproducible method to extract high quality genomic DNA using formaldehyde. Formaldehyde cross linking has been widely used to study binding of specific proteins to DNA elements in intact cells. Here we show that intact good quality high molecular weight DNA can be extracted from the formaldehyde- DNA cross linking. Normal quantity was obtained by this method. The DNA was extracted from paddy grasshopper ( Gastrimargus musicus )in a single step. The method does not require expensive and environmentally hazardous reagents and equipment. It can be performed even in low technology laboratories. The amount of tissue required by this method is less.
Keywords:- Grasshopper, formaldehyde, DNA.
Research article:-Medical microbiology
*Laghawe Avinash R1, MS. Jaitly Neelam K2, Thombare Vilas R3.
*1M.D. (Microbiology), Asst. Professor, Dept. of microbiology,Sri Aurobindo medical college and P. G. Institute, Indore, M.P., India.
2M.D. (Microbiology), Professor, 3 Professor and HOD, Dept. of Microbiology, N.K.P. Salve Institute of medical sciences, Nagpur, Maharashtra, India.
Abstract:- Purpose: The purpose of this study was to detect the prevalence of AmpC Beta-lactamases in Gram-negative bacilli. Methods: Screening for AmpC was done by using various third generation cephalosporins (3GC) and cefoxitin. Organisms resistant to cefoxitin and reduced susceptibility to 3GCs were tested for presence of AmpC by AmpC disc test. Results: Total 432 isolates were isolated from 414 samples. Out of 432 isolates, 69(15.97%) were (48 pure and 21 mixed) AmpC β-lactamases and 21(4.86%) isolates were positive for both ESBL and AmpC. Out of 48 pure AmpC producers, 47.91% (23/48) were Inducible AmpC β-lactamases. Out of 48 pure AmpC producers, 42(87.5%) were from indoor patients and 6(12.5%) were from outdoor patients. Of the mixed beta lactamases (ESBL + AmpC), all (21) were from indoor patients. Conclusion: Regular monitoring of the incidence of the AmpC Beta-lactamase production by organisms is necessary. As the Beta-lactamase producing organisms are also present in outdoor patients, they also should be screened for Beta-lactamases. As high-level expression of AmpC Beta-lactamases may mask recognition of ESBL, detection of AmpC Beta-lactamase should be done along with ESBL detection. Inducible AmpC Beta-lactamases can lead to transmission of drug resistance to other bacteria through plasmids.
Keywords:- AmpC, Cefoxitin, Third generation cephalosporins(3GC), Beta lactamases.
Research article:-Biochemistry
®Nwaoguikpe R N1, Ujowundu CO1, Wesley B 2
1Department of Biochemistry, Federal University of Technology, P.M.B. 1526,Owerri,Imo State,Nigeria.
2Department of Microbiology, Federal University of Technology, P.M.B.1526,Owerri,Imo State, Nigeria.
Abstract:- The phytochemical and biochemical compositions of African walnut (Tetracarpidum conophorum) were determined for two samples of the variety-the boiled and mashed wet nuts, and the dried powdered nuts. Results of phytochemical analysis revealed the presence of the following expressed as percentage. For the mashed wet nuts: Saponins (8.37±0.1); flavonoids (3.20 ±0.2); phenols (1.90±0.1), tannins (0.51±0.0) and alkaloids (0.41±0.1). For the dried and powdered variety, the results are- Saponins (5.03±0.2); flavonoids (2.70 ±0.1), tannins (0.45±0.1), phenols (1.51±0.1) and alkaloids (0.350±0.1). Proximate compositional analysis expressed as percentage shows the following results: moisture (45.01±0.1)WM,(2.88±0.02)DP; Crude fat (13.81±0.01) WM, (32.21±0.01) DP; Crude protein (18.75±0.01) WM, (13.72±0.02 ) DP;CHO (18.43±0.1) WM,(46.38±0.2) DP; Crude fiber (1.95±02) WM,(1.72±0.02 ) DP; Ash (1.91±0.01) WM,(3.11±0.01) DP, FE( 273.01±0.1) WM, (530.29±0.02) DP. The values of the vitamins are expressed in mg/ml as shownbelow:VitaminA is expressed in µg/ml(1283.33±1.18)DP, (285.60±0.2)WM;Vit.C (14.80±0.02)DP,(17.57±0.01)WM;riboflavin (0.12±0.02)DP,(0.12±0.02)WM;Thiamine(B1) (0.20±0.01)DP,(0.12±0.01)WM; Niacin(2.81±0.01)DP,(2.91±0.01).The mineral composition of the nut is very outstanding and not significantly affected by the processing methods. The values of the minerals are shown as follows: Mg (0.36±0.01) DP.,(0.31±0.01)WM; Ca (2.10±0.2)DP, (1.88±0.1)WM; P(0.35±0.01)DP, (0.36±0.01)WM and others. The phytochemical and nutrient compositions of this tropical nut are expository and depicts the role of the seeds in nutrition and health.
Keywords:-Biochemical composition, phytochemicals, African walnut.
Original research article:-Forensic medicine
S Manjunath1,*Shankar M Bakkannavar2,Pradeep Kumar G3,Vrinda J Bhat4,Nayana Prabhu5,Asha Kamath6,RaghavendraBabu Y P7
1Associate Professor, Department of Forensic Medicine, Kasturba Medical College, Manipal University, Manipal, India.
2Assistant Professor, Department of Forensic Medicine, Kasturba Medical College, Manipal University, Manipal, India.
3Professor and Head, Department of Forensic Medicine, Kasturba Medical College, Manipal University, Manipal, India.
4Professor and Head, Department of Forensic Medicine, Shimoga Institute of Medical Sciences, Shimoga,India.
5Associate Professor, Department of Prosthodontics, Manipal College of Dental Sciences, Manipal University, Manipal, India.
6Selection Grade Lecturer, Department of Community Medicine, Kasturba Medical College, Manipal University, Manipal, India.
7Associate Professor, Department of Forensic Medicine, Kasturba Medical College,Mangalore,ManipalUniversity,India.
Abstract:- Palatoscopy or Palatal rugoscopy is the name given to the study of palatal rugae in order to establish a person’s identity. Rugoscopy finds application in the field of anthropology, comparative anatomy, genetics, forensic odontology, prosthodontics and orthodontics. The present study is an attempt to determine the differences in the rugae pattern among the Indian males and females in a small portion of a population. 63 subjects, 32 males and 31 females in the age group 17-25 years formed the materials for the study. After a written informed consent, maxillary dental casts were taken and interpretation of the rugae pattern was done as per classification by Thomas and Kotze. It was noted that the Indian females had more number of rugae on the right side of the palate than the male counterparts. Curved and straight shapes were predominant among the females whereas the wavy pattern was more common among the males. Their uniqueness to individuals has been recognized as providing a potentially reliable source of identification.
Keywords:-Forensic odontology; Identification; rugoscopy.