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Case study:-General surgery
*Satya Ranjan Patra1, V Srinivas2, GL Prasad3
1Associate Professor in General Surgery ,Madha Medical College And Hospital ,Thandalam Kovur Chennai 600122.India.
2Assistant Professor,3 Professor & HOD , General Surgery, PSI Medical College & Hospital ,Chinnaoutapalli Ganavaram, Andhra Pradesh 521286,India.
Abstract:- A case with recurrent small bowel obstruction, which was operated for a small bowel tumour four years ago, was operated upon and was found to be suffering with enteric stricture; enterocolic fistula and multiple mesenteric lymph nodes. Intestinal obstruction was managed by resection of the lesions and primary anastomosis of the intestine. Histo pathology revealed recurrent small bowel adenocarcinoma with lymphnode metastasis. Patient underwent 5- FU based chemotherapy and survived for twenty months.
Keywords:- Recurrent Small bowel adenocarcinoma, malignant obstruction, adjuvant chemotherapy.
Research article:-Biochemistry
®Nwaoguikpe R N1, Ujowundu CO1, Wesley B 2
1Department of Biochemistry, Federal University of Technology, P.M.B. 1526,Owerri,Imo State,Nigeria.
2Department of Microbiology, Federal University of Technology, P.M.B.1526,Owerri,Imo State, Nigeria.
Abstract:- The phytochemical and biochemical compositions of African walnut (Tetracarpidum conophorum) were determined for two samples of the variety-the boiled and mashed wet nuts, and the dried powdered nuts. Results of phytochemical analysis revealed the presence of the following expressed as percentage. For the mashed wet nuts: Saponins (8.37±0.1); flavonoids (3.20 ±0.2); phenols (1.90±0.1), tannins (0.51±0.0) and alkaloids (0.41±0.1). For the dried and powdered variety, the results are- Saponins (5.03±0.2); flavonoids (2.70 ±0.1), tannins (0.45±0.1), phenols (1.51±0.1) and alkaloids (0.350±0.1). Proximate compositional analysis expressed as percentage shows the following results: moisture (45.01±0.1)WM,(2.88±0.02)DP; Crude fat (13.81±0.01) WM, (32.21±0.01) DP; Crude protein (18.75±0.01) WM, (13.72±0.02 ) DP;CHO (18.43±0.1) WM,(46.38±0.2) DP; Crude fiber (1.95±02) WM,(1.72±0.02 ) DP; Ash (1.91±0.01) WM,(3.11±0.01) DP, FE( 273.01±0.1) WM, (530.29±0.02) DP. The values of the vitamins are expressed in mg/ml as shownbelow:VitaminA is expressed in µg/ml(1283.33±1.18)DP, (285.60±0.2)WM;Vit.C (14.80±0.02)DP,(17.57±0.01)WM;riboflavin (0.12±0.02)DP,(0.12±0.02)WM;Thiamine(B1) (0.20±0.01)DP,(0.12±0.01)WM; Niacin(2.81±0.01)DP,(2.91±0.01).The mineral composition of the nut is very outstanding and not significantly affected by the processing methods. The values of the minerals are shown as follows: Mg (0.36±0.01) DP.,(0.31±0.01)WM; Ca (2.10±0.2)DP, (1.88±0.1)WM; P(0.35±0.01)DP, (0.36±0.01)WM and others. The phytochemical and nutrient compositions of this tropical nut are expository and depicts the role of the seeds in nutrition and health.
Keywords:-Biochemical composition, phytochemicals, African walnut.
Research article:-Chemistry
*A. Kalaiselvan1, K. Gokulakrishnan2, T. Anand1 .
1Centre for Research and Development, PRIST University, Vallam, Tanjavur – 613 403, Tamilnadu, India
2Department of Chemistry, PRIST University, Vallam, Tanjavur – 613 403, Tamilnadu, India.
Abstract:- Andrographis paniculata is traditionally used for treating different aliments. The present investigation was carried out to determine the possible chemical components from Andrographis paniculata by GC-MS technique. This analysis revealed that the ethanol extract contains 2,5-Octadecadiynoic acid, methyl ester (27.83%), 1,2,3,5-Cyclohexanetetrol, (1à,2á,3à,5á)-(14.78%), 10-12-Pentacosadiynoic acid (12.25%), 2H-Pyran, 2-(7-heptadecynyloxy)tetrahydro-(7.69%), 9,12,15-Octadecatrienoic acid, 2-[(trimethylsilyl)oxy]-1-[[(trimethylsilyl)oxy]methyl]ethyl ester, (Z,Z,Z)-(6.98%) 1,3-Propanediol, 2-(hydroxymethyl)-2-nitro-(6.28%), 3-O-Methyl-d-glucose(5.36%), Phytol(2.63%).
Keywords:-Andrographis paniculata, GC-MS analysis, Acanthaceae.
Research article:- Medical microbiology
*Sangamesh M Malaji1., Gopal Bajaj.1 S.A.Kora.2, Satish Biradar.2, Renuprasad Chikkamath.3 1Assistant Prof., 2 Associate professor, 3 PG student, Department of Microbiology, S.Nijalingappa Medical College and H.S.K.Hospital & Research Center, Bagalkot, India.
Abstract:- Background and objectives: One of the common complaints of HIV infected patients is diarrhoea. Enteric parasites are major cause of diarrhoea in HIV infected individuals. The present study was undertaken to detect enteric parasites in HIV infected patients with diarrhoea at different levels of immunity. Methods: The study was carried out at S.N.M.C & H.S.K Hospital between June 2011to March 2012 among consecutively enrolled 50 HIV infected patients presenting with diarrhoea. Stool samples were collected and examined for enteric parasites by microscopy and special staining methods. Results:- Intestinal parasitic pathogens were detected in 44% patients, and the major pathogens included Cryptosporidium parvum (20%) the most common followed by Isospora belli (10%), Entamoeba histolytica (8%). In HIV infected patients with CD4 count <200cells/µl, C. parvum was the most commonly observed(54%) pathogen. Proportion of opportunistic pathogens in patients with CD4 count <200cells/µl was higher as compared with other two groups of patients with CD4 count 200-400 and CD4 count >400cells/µl. Interpretation and conclusions: Parasitic infections were detected in 44% HIV infected patients and low CD4 count was significantly associated with opportunistic infection. Detection of etiologic pathogens might help clinicians decide appropriate management strategies.
Key Words:- Diarrhoea- HIV – opportunistic parasites.
Research article:-
Das S, Tiwari K L, Sen S, * Singh A. G D. Rungta College of Science and Technology, Kohka, Kurud road, Bhilai, (C.G.),India.
Abstract:-DNA extraction is an important step in molecular assays and plays a vital role in obtaining high resolution results in gel-based systems. So far Many genomic DNA extraction protocols have been described. Here a rapid DNA extraction method is described which is a very simple, fast, universally applicable and reproducible method to extract high quality genomic DNA using formaldehyde. Formaldehyde cross linking has been widely used to study binding of specific proteins to DNA elements in intact cells. Here we show that intact good quality high molecular weight DNA can be extracted from the formaldehyde- DNA cross linking. Normal quantity was obtained by this method. The DNA was extracted from paddy grasshopper ( Gastrimargus musicus )in a single step. The method does not require expensive and environmentally hazardous reagents and equipment. It can be performed even in low technology laboratories. The amount of tissue required by this method is less.
Keywords:- Grasshopper, formaldehyde, DNA.