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Research article:- Biochemistry, & Bioinformatics
Paramasivam Ragavendran1, Murugan Muthu1, Chintamony Arul Raj1, Dominic Sophia1, Thangarajan Starlin2, Balasubramanian Vidya1, Palanisamy Chella Perumal2 & Velliyur Kanniappan Gopalakrishnan1,2*.
1Department of Biochemistry, Karpagam University, Coimbatore, Tamilnadu, India 641 021. 2Department of Bioinformatics, Karpagam University, Coimbatore, Tamilnadu, India, 641 021.
Abstract:- Aerva lanata (A. lanata) L. is an ayurvedic medicinal plant, found throughout tropical India as a common weed in fields and wasteland. In this study, to evaluate the HPTLC fingerprinting analysis of bioactive compounds for the identification and conformation analysis of aqueous, ethanol and aqueous ethanol extract of A.lanata. Quantification of these bioactive compounds are carried out, after that these extracts undergo the analysis of HPTLC profile of flavanoid, phenols, tannins, terpenoids and glycosides. All the three extracts of A.lanata has flavanoid, phenolics, tannins, terpenoids and glycosides. But terpenoids are not present in aqueous extract. The present study supplements the folkloric usage of the studied plants which possess known and unknown bioactive compounds with bioactivity. By isolating and identifying of these compounds can be formulated to treat diseases. This plant can be utilized as useful herb for alleviation of various illness and disorder.
Key words:- Aerva lanata, HPTLC fingerprinting, Flavanoid, Phenolics, Tannins
Case Report:- Ophthalmology
Lokesh H M1* and Bindurani M K2.
1MBBS,MS. Ophthalmology, Associate professor, Department of Ophthalmology,2MBBS,MD Anatomy, Assistant Professor, Department of Anatomy, Sri Siddhartha Medical College,Tumkur-572107, Karnataka State, India.
Abstract:- The Congenital lacrimal system anomalies other than nasolacrimal duct obstruction are uncommon. Hereditary lacrimal fistula is a rare developmental anomaly. We report three patients with congenital lacrimal fistulae in the same family. A female patient aged about 23 years presented with history of epiphora from both eyes since childhood. On examination small orifice was found on either side of the nose near the medial canthus of both the eyes. There was minimal mucopurulent discharge from the orifice. The lacrimal puncta were absent. There were no other ocular abnormalities except hypertelorism.The diagnosis was made as congenital bilateral lacrimal fistula. The two twin children of the patient also had same complaints. There was History of consanguinity. Karyotyping from peripheral blood of all the affected patients was done. The presence of lacrimal fistula is an indication for the search of systemic and ocular manifestations in affected patients.
Key words:- Hereditary, Hypertelorism, karyotype, Lacrimal fistula, ,manozygotic twins.
Short communication:-Microbiology
Bindu D*, Illamani V, Chitralekha S, Kiran Madhusudhan, Praveena R.
Department of Microbiology, Sree Balaji Medical College & Hospital, Chromepet Chennai (Bharath University),Chennai,India.
Abstract:- Urinary tract infection (UTI) is a common community acquired infection. Due to rising antibiotic resistance among uropathogens, especially in gram negative bacteria, it is important to have knowledge of antibiogram of these organisms. This study retrospectively analyses the antibiogram among the gram negative urine isolates during 7 months in patients with UTI. Out of 59 uropathogens, the common isolates were Escherichia coli 41 (69%), Klebsiella spp. 15(21%) and Pseudomonas spp. 3 (5%). The results have showed high level of susceptibility to aminoglycosides . More 40 isolates were resistant to cephalosporins, aztreonam, norfloxacin, nalidixic acid, ciprofloxacin and ofloxacin. This result shows high level of resistance to cephalosporins.
Keywords:- Urinary tract infection (UTI), Extended Spectrum β-Lactamases (ESBL), Antibiogram.
Research article:-Pharmacology
Farhana R1*, Arul Amutha E2, Glory J2, Inbaraj SD2, Muniappan M3.
1Assistant Professor,2Associate Professor,3Professor, Dept. of Pharmacology, Sree Balaji Medical College and Hospital, Chennai, Tamilnadu, India.
Abstract:- Aim: To study the antinoceciptive and anti-inflammatory activity of ethanolic extract of Lycopersicon esculentum Mill, on different experiment models. Materials and Methods: Wister albino rats and Swiss albino mice were used for studying antinoceciptive and anti-inflammatory activity of ethanolic extract of Lycopersicon esculentum at doses 100 mg/kg i.p. and 200 mg/kg i.p. Various models viz. acetic acid induced writhing model (mice), Eddy’s hot plate (rats) and formalin-induced paw licking model (rat) for antinoceciptive study and carrageenin induced paw oedema model (rat) for anti-inflammatory study. Results: In all the models for antinoceciptive study ethanolic extract of Lycopersicon esculentum (100 mg/kg i.p) showed significant decrease in pain sensation when compared to experimental control and standard drug. For anti-inflammatory study, ethanolic extract of Lycopersicon esculentum (100 mg/kg, 200 mg/kg i.p) showed significant decrease in inflammation when compared to control and standard drug. Conclusion: Ethanolic extract of Lycopersicon esculentum showed analgesic (central and peripheral) and anti-inflammatory activity.
Key words:- Antinoceciptive, Lycopersicon esculentum, Formalin test, Anti-inflammatory carrageeni.
Case report-Microbiology
Manisa Sahu1*, Sujatha S2, Sadia Khan3 & Subhash C Parija4. *1Ex PG student ; Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India. 2Professor, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India. 3Ex PG student ; Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India. 4Professor and Head, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
Abstract:- Candida species, especially non albicans species have replaced Candida albicans as the predominant opportunistic pathogen in more than half of the cases of candidal infections in compromised patients. A 35 year old male was admitted to the orthopedics ward after sustaining patellar fracture on the left leg following a road traffic accident developed compartment syndrome. He was managed conservatively with antibiotics and rest. Gradually he developed gangrene of the affected part. Patient was otherwise immunocompetent. Both aerobic and anaerobic bacterial culture of tissue bit did not show any growth twice. Subsequent aerobic culture on blood agar showed yeast like colonies identified as Candida zeylanoides, a rare isolate, sensitive to fluconazole and amphotericin B. Symptoms improved after treatment with fluconazole and patient was discharged. The changing scenario of candidiasis emphasizes the definitive identification to species level and determination of its antifungal susceptibility pattern for proper management of the infections.
Key words:- Candida albicans, Candida zeylanoides, gangrene and fluconazole.