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Research article:-Zoology
Eshita Pandey, Anjali Srivastava & Sabina Khanam
Department of Zoology, Dayanand Girls P.G. College, Kanpur, Uttar Pradesh, India.
Abstract:- There are psychological, physical, and biological stressors in abundance in our environment and together with extreme environmental conditions they have significant negative effects on physiological and life history traits of organisms. The present study focusses on starvation as a stress factor on a species of Quails- Coturnix coturnix. Anthropogenic factors have reduced both feeding sites and food for animals forcing them to starve unnaturally and this led to choice of starvation as a factor for study. The study categorized the birds into two groups, non-stressed or the control group and stressed or the experimental group which were provided with water but no food. Following the completion of experiment serum was separated and electrophoresis (SDS- PAGE) was performed on the samples after which gel surfaces were photographed and analyzed for the bandwidth of the serum proteins. Birds showed normal behavior initially and their water intake was normal, later their excreta increased, they became restless and their water consumption increased significantly. The birds also showed puffing. Four bands on the whole in Coturnix coturnix were seen to be present in the control samples and not traced in the experimental samples; similarly one band could be traced in the experimental samples and not in the control sample. Three bands are found to be thicker in the experimental samples as when compared to the control sample which indicate towards proteins whose synthesis were enhanced under conditions of stress. One band in the experimental sample showed a change in position with the corresponding control sample indicating towards a change in molecular weight of the protein concerned due to stress.
Keywords:- Anthropogenic factors, Stressors, Coturnix coturnix, Starvation, SDS-PAGE.
Case report-Microbiology
Manisa Sahu1*, Sujatha S2, Sadia Khan3 & Subhash C Parija4. *1Ex PG student ; Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India. 2Professor, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India. 3Ex PG student ; Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India. 4Professor and Head, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
Abstract:- Candida species, especially non albicans species have replaced Candida albicans as the predominant opportunistic pathogen in more than half of the cases of candidal infections in compromised patients. A 35 year old male was admitted to the orthopedics ward after sustaining patellar fracture on the left leg following a road traffic accident developed compartment syndrome. He was managed conservatively with antibiotics and rest. Gradually he developed gangrene of the affected part. Patient was otherwise immunocompetent. Both aerobic and anaerobic bacterial culture of tissue bit did not show any growth twice. Subsequent aerobic culture on blood agar showed yeast like colonies identified as Candida zeylanoides, a rare isolate, sensitive to fluconazole and amphotericin B. Symptoms improved after treatment with fluconazole and patient was discharged. The changing scenario of candidiasis emphasizes the definitive identification to species level and determination of its antifungal susceptibility pattern for proper management of the infections.
Key words:- Candida albicans, Candida zeylanoides, gangrene and fluconazole.
Research article:-Pharmaceutical Sciences
Shinya RV, Jomy Jose and Padmaja.V*.
College of Pharmaceutical Sciences, Medical College, Thiruvananthapuram – 695011, Kerala,India.
Abstract:- Naregamia alata ( W&A) ,a small branching undershrub, used to cure asthma, bronchitis, biliousness, and ulcers. This work covers studies on pharmacognostical and biotechnological studies on the leaves of Naregamia alata (W&A).Pharmacognostical studies were made on macroscopical characters and microscopical characters of leaves, microscopical features of powdered drug, ash values, extractive values, loss on drying, leaf constants, behaviour of crude drug powder with different chemical reagents and its fluorescence analysis. This will help for correct identification of the plant for the future references. Tissue culture studies showed that leaves of Naregamia alata (W&A) can be artificially cultured to plantlets in MS media supplemented with appropriate concentration of IAA and BAP. DNA barcoding studies showed that matK (785 bp), rbcL (553 bp) and ITS (722 bp) can be used as barcodes for the identification of Naregamia alata (W&A) from other species in Meliaceae family.
Key Words:- Naregamia alata, leaves, pharmacognostical and biotechnological studies, (785 bp), rbcL (553 bp), ITS (722 bp), DNA barcoding.
Review article:-Periodontics
Dr. Sandeep A. Lawande MDS, FICOI (USA), FICD, FPFA
Assistant professor, Department of Periodontics, Goa Dental College & Hospital, Bambolim, Goa,India.
Abstract:- Obesity is a growing medical problem worldwide. Obesity is a systemic disease that predisposes to a variety of co-morbidities and complications that affect overall health. Cross-sectional studies suggest that obesity is also associated with oral diseases, particularly periodontal disease, and prospective studies suggest that periodontitis may be related to cardiovascular disease. This article explores the multidirectional relationship between obesity and periodontal disease.
Key words:- Obesity, periodontal disease, inflammation.