DocumentsDate added
Research article:-
Leila Ksiaa Cheikhrouhou (MD), Yousr Lakhoua Gorgi (phD), Salwa Jendoubi Ayed (MD), Houda Aouadi (MD) , Imen Sfar (MD), Mouna Makhlouf (MD), Taieb Ben Abdallah (phD), Khaled Ayed (phD).
Immunology Research Laboratory of Kidney Transplantation and Immunopathology (Laboratoire de Recherche: LR03SP01). University Tunis El Manar; Charles Nicolle Hospital. Tunisia.
Abstract:-
The aim of this study was to investigate the possibility of a significant relationship between the spontaneous clearance or the persistence of HCV infection and chemokines , chemokine receptors, cytokines and apoptosis genes polymorphisms in Tunisian hemodialysed patients. The polymorphisms of genes: CCR5 Δ32, CCR5 (-59029) A/G, CCR2 (64Ile), and MCP-1(-2518) A/G, (-889) IL-1α, (-511) and (+3954) IL-1β, IL-1-Ra, IL-18 (-137) and (-607), IL-12p40 (-1188) and Apo1/Fas (-670) were determined by PCR-RFLP, PCR-SSP and PCR-VNTR, at healthy blood donors (100 for cytokines and apo/fas gene polymorphisms and 170 for chemokine gene polymorphisms) and 100 hemodialysed patients infected by the HCV. Patients were classified in two groups: G1 included 76 active chronic hepatitis patients (positive RNA-HCV) and G2 included 24 having eliminated spontaneously virus (negative RNA-HCV). The univariate analyze of the genotypes and alleles frequencies of the cytokines polymorphisms studied does not reveal any positive or negative association statistically significant with the outcome of the HCV infection. Nevertheless, the frequency of genotype association [-37GC/-607CA] IL-18 is statistically higher among G2 patients (41,7%) compared to that at G1 hemodialysed (15,8%) (p=0,008, OR: 0.26, 95%CI: [0.10-0.73]). We found also, a significant increased frequency of AA genotype of Apo1/Fas gene in G2 patients (41,6%) than in G1 (17,5%) (p=0,026, OR=3,49, 95% CI [1,13-10,69]). Adjustment for known covariates factors (age, gender and genotypes) confirmed these univariate findings and revealed that the genotype association GC-CA of (-137and-607) IL-18 gene and AA genotype of Apo1/Fas gene were associated to the clearance of HCV (p=0.041 and p=0.017 respectively). Our results showed also a statistically increased frequencies of the CCR2 (64Ile) and the (-59029) CCR5 A alleles in total patients HCV infected (22,1% and 35,9%) and in G1 (24,3% and 40,6%) compared to controls (14,4% and 20%)[p=0.0086, p=0.03 and p=0.04, p=0.017 respectively]. Also, we observe a lower frequency of the MCP-1 G allele and a greater frequency of the CCR5 Δ32 variant in G2 (15,2% and 6,5%) compared to G1(22,6% and 1,4%)[p>0.05], but adjustment for known covariates factors didn’t confirmed these univariate findings. In conclusion, our study suggests a possible role of some of the chemokine and cytokine and apo/fas polymorphisms studied in the outcome of HCV infection in Tunisian population. These results should be further investigated by large population-based studies.
Key words:- Hepatitis C virus, Spontaneous clearance, Chemokines, Chemokines Receptors genes polymorphisms, cytokines, apo/fas polymorphisms.
Copyright © 2013 Leila K Cheikhrouho et al., This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article:-Anaesthesia
R.Uma1, Meera Rani Nayak2 & Hansa Jayakumar3
1,2Assisstant professor, 3Professor, Department of anaesthesia, Sree Balaji Medical College and Hospital, Chennai, India.
Abstract:-
Clonidine is an alpha2 adrenorecptor agonist which has become a popular adjuvant in anaesthesia. Its effectiveness in providing better intraoperative hemodynamics has been well proven. In our study we show the effectiveness of bolus doses of iv clonidine without a follow up infusion in providing better hemodynamic stability in laparoscopic cholecystectomy. METHODS: We did a randomised placebo controlled study to assess the effect of intravenous clonidine on intraoperative hemodynamics in 40patients who underwent elective laparoscopic cholecystectomy. Patients were randomised into 2 groups. Group C received iv clonidine at a dose of 3micrograms/kg over 15mts prior to induction and Group P recieved same volume of NS(normal saline).Anaesthesia was induced and maintained with propofol, fentanyl and vecuronium bromide, isoflurane and nitrous and oxygen 2:1. Pulse rate(PR) and mean arterial pressure(MAP) were recorded prior to induction, 2mts after intubation, before pneumoperitoneum, 10 and 20mts after pneumoperitoneum and 10mts after extubation. Post operative recovery was assessed using the ramsay sedation score at 30mins, 60mins and 120mins. The occurrence of adverse effects such as nausea, vomiting, hypotension, bradycardia and respiratory depression where also noted. RESULTS AND DISCUSSION: Patients in group C maintained greater intraoperative hemodynamic stability. There was significant decrease in PR and MAP in the Group-C during pneumoperitoneum and after extubation.
Key words:- Clonidine, laparoscopic cholecystectomy, hemodynamic stability.
References:
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Copyright © 2013 R.Uma, Meera Rani Nayak & Hansa Jayakumar. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original research article:-
Porwal Sanjay K1*,Gupta Ritu 2,Swarnkar Madhusudan3,Baig Vaseem N.4,Deewan Rishi5 & Jain Chetna6.
1Associate Professor, Department of Surgery, 2Associate Professor, Department of Obstetrics and Gynecology, 3Assistant Professor, 4Associate Professor, Department of P.S.M., 5Professor, 6Assistant Professor, Department of Pathology, Jhalawar Medical College, Jhalawar, Rajasthan- 326001,India.
Abstract:- Background: The Fine needle aspiration cytology is now an investigation of choice in evaluating thyroid swellings. It is simple, safe, cost effective and easy to do as out patient procedure. It is accurate, specific and sensitive. The objective of this study was to determine the reliability of FNAC in detecting various benign and malignant lesions of thyroid and correlate the results with histopathological examination report. Materials and Methods: This study was carried out at the Department of Surgery, S.M S. Medical College Hospital, Jaipur, Rajasthan (India). FNAC was performed on 100 patients of all age group and both sexes who presented with thyroid swellings. Results: Out of 100 patients, 87 were females and 13 were males. Majority of patients came with swelling of duration of less than a year. Majority cases came without any predominant symptoms. All cases underwent surgery and finally histopathological report collected. The goiter seen in 47, adenoma in 33, cancer in 13 and 7 histopathological reports were miscellaneous like lymphoid hyperplasia, hashimotos disease. Majority of population was iodine salt user (75%). Key words:- Fine Needle Aspiration Cytology, Thyroid swelling, Sensitivity, Specificity, Accuracy, Histopathology.
References:-
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Copyright © 2013 Sanjay K Porwal. et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Case report:- Microbiology
B.Kiran Madhusudhan1* & B.Madhusudhan2
1MD (Microbiology), Associate Professor in Microbiology, Barath University, Shree Balaji Medical College and Hospital, Chrompet, Chennai-600 044, India.
2MS.,M.Ch(Plastic)-Consultant Plastic Surgeon BRS Hospital Pvt Ltd, Nungambakkam, Chennai-34, India.
Abstract: Protein C deficiency is a common cause for hypercoagulable state and can be inherited or acquired. Early in warfarin treatment, serum levels of vitamin K dependent antithrombotic protein C falls, even before other clotting factors. This causes a temporary prothrombic state, leading to venous thrombosis in subcutaneous and dermal vessels causing haemorrhagic infarction and skin necrosis. We report here a post aortic valve replacement patient who presented with a painful necrotic skin lesion of 2 weeks duration on the medial aspect of his right thigh .Patient was on anticoagulant treatment (T.Acitrom-5mg od) and his protein C level was well below normal limits, which helped in the diagnosis of warfarin induced skin necrosis in this case. Reversal of warfarin therapy was adopted, followed by surgical debridement and skin grafting.
Key words:- Skin necrosis, Warfarin, Hypercoagulable state, Protein C deficiency.
References:-
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Copyright © 2013 B.Kiran Madhusudhan & B.Madhusudhan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Case Report:-Otorhinolaryngology
Dutta Sumanta K1* , Nandy Sumit2 , Chakraborty Debdulal3 & Basu Keya4.
1Associate Professor, 3Assistant Professor, Department of Otorhinolaryngology, ,2Demonstrator, 4Professor & Head, Department of Pathology, Calcutta National Medical College & Hospital, Kolkata, West Bengal, India.
Abstract:- Rhinosporidiosis is a chronic granulomatous disease of the muco-cutaneous tissue, which clinically presents as polypoidal growths. Cutaneous lesions are infrequent and are generally associated with mucosal lesions. We present a case of scalp rhinosporidiosis in association with recurrent nasopharyngeal rhinosporidiosis in a 52 year old male patient. He presented with nasal mass, nasal obstruction, episodes of epistaxsis and dysphagia to solid foods. This was accompanied with two small flesh coloured warty polypoidal lesions over the scalp. Past history revealed similar type of nasal complaints for last 35 years for which he was operated 13 times before in different hospitals! Histopathology of the cutaneous and nasopharyngeal masses revealed numerous sporangia containing spores of various sizes in a vascular connective tissue, confirming the diagnosis of cutaneous and nasopharyngeal rhinosporidiosis. Because of rarity of skin involvement (particularly scalp) and noting the high frequency of recurrence of this disease, this case has been reported.
Key words:- Cutaneous rhinosporidiosis, Nasopharyngeal, Recurrence, Scalp.
References:-
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Copyright © 2013 Dutta Sumanta K et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.