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Research article
Eugène Sèlidji Attakpa1, 3*, Alphonse Sezan1, Bialli Seri2, Naim Akhtar Khan3
Affiliation:-
1Laboratoire de Biomembranes et Signalisation Cellulaire,Département de Physiologie Animale, Faculté des Sciences et Techniques 01 BP : 4521 Université d’Abomey Calavi Cotonou (Rép. du Bénin).
2Laboratoire de Neurosciences,Unité de Formation Biosciences 22 BP 582 Abidjan 22 Université de Cocody-Abidjan (Rép. de Côte-d’Ivoire).
3Unité Propre de Recherche de L’Enseignement Supérieur EA 4183 Lipides et Signalisation Cellulaire, Faculté des Sciences de la Vie, Université de Bourgogne, 6 Boulevard Gabriel, 21000 Dijon, (France).
Abstract:
In order to investigate the implication of docosahexaenoic acid (DHA) in T signaling, we assessed their effects on the activation of two mitogen activated protein (MAP) kinases, i.e. extracellularly-regulated kinases 1 and 2 (ERK1/ERK2) in mouse CD4+ T cells. The n-3 polyunsaturated fatty acids (PUFAs) alone failed to induce MAP kinase (MAPK) enzyme activity. To elucidate whether DHA act via protein kinase C (PKC) dependent and independent pathways, we employed their respective activators, i.e. phorbol 12-myristate 13-acetate (PMA) and anti-CD3 antibodies. DHA diminished the PMA- and anti-CD3-induced phosphorylation of ERK1/ERK2 CD4+ T cells. In the present study, PMA acts mainly via protein kinase C (PKC) whereas anti-CD3 antibodies act via PKC-dependent and–independent mechanisms. We observed that DHA inhibited PMA-stimulated PKC enzyme activity. Together these results suggest that DHA modulate ERK1/ERK2 activation upstream of MEK via PKC-dependent and independent pathways and that these actions may be implicated in DHA-induced immunosuppression. Our study shows that DHA inhibits cell cycle progression.
Key words: Mouse; CD4+ T cells; DHA, ERK.
References:
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Article citation:-
Eugène Sèlidji Attakpa,Alphonse Sezan,Bialli Seri,Naim Akhtar Khan. Action of Docosa-Hexaenoic Acid is their interference with MAP kinase signaling in mouse CD4+ T cells. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 September; 34(34):1704-1710.Available at http: //www.jpbms.info.
Copyright © 2013 Eugène Sèlidji Attakpa,Alphonse Sezan,Bialli Seri,Naim Akhtar Khan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Sinha Abhishek1*,Srivastava Sunita2, Mishra Anuj3, Agarwal Nitin4 & Pooja M. Sinha5
Affiliation:-
1Reader,2Senior Lecturer, 3Post graduate student, 4Professor & Head, Department of Oral Medicine and Radiology, 5Senior lecturer, Department of public health dentistry, Sardar Patel Post Graduate Institute of Dental & Medical Sciences, Raebareily Road, Lucknow, Uttar Pradesh, India.
Author’s contributions:- All the authors contributed equally to this paper.
Work attributed by: Department of Oral Medicine and Radiology, Sardar Patel Post Graduate Institute of Dental & Medical Sciences, Raebareily Road, Lucknow, Uttar Pradesh, India.
Abstract:
Background: Oral Lichen planus is a chronic inflammatory disorder that can be painful especially in erosive forms that commonly affects skin and oral mucosa. In this area different treatments have been used in symptomatic oral lichen planus (OLP), with variable results.
Aim: To compare the efficacy of aloevera and topical steroids in symptomatic treatment of Erosions and burning sensation in Erosive Lichen Planus.
Methods: A total of 10 patients with Oral Lichen Planus were randomly selected for the study and divided into two groups (A and B). The group A received aloe vera gel and group B topical steroids. The patients were under regular follow up every 2 weeks for 3 months. Clinical evaluation and pain reduction was evaluated by VAS method in group A and B. Data were evaluated statistically by using Wilcoxon signed rank test.
Results: In group A, 4 patients had complete remission and 1 patient showed good response to treatment where as in group B, 2 patients had complete remission, 2 patient showed good response and 1 patient showed no response to treatment (p<0.001), it was determined that Aloevera was stastistically significant (p<0.05) as compared to Topical Steroid at the end of treatment.
Conclusion: The topical application of Aloe vera improves the total quality of life score in patients with oral lichen planus. However, according to result of this study, the use of aloevera gel instead of steroid in treating erosive lichen planus is better and more appropriate choice.
Key words: Aloevera gel; Burning sensation; Oral lichen planus; Triamcinolone acetonide.
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Article citation:-
Sinha Abhishek,Srivastava Sunita, Mishra Anuj, Agarwal Nitin & Sinha M Pooja. Aloevera vs topical steroid in treatment of erosive lichen planus. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 September; 34(34): 1657-1662.Available at http://www.jpbms.info
Copyright © 2013 Sinha Abhishek,Srivastava Sunita, Mishra Anuj, Agarwal Nitin. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
1Varsha Chaudhary, *2Bhat Kishore,3Sunil Rao,4Preeti Ingalagi & 5Manohar Kugaji
Affiliation:-
1,5Research Officer,2Professor and Head,4Sr. Lecturer, Department of Microbiology, Maratha Mandal’s NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India.
3Professor and Head, Department of Microbiology, Yenepoya Medical College, Yenepoya University, Mangalore, India.
Author’s contributions: - All the authors contributed equally to this paper.
The name of the Department and Institution to which the work should be attributed:-
Department of Microbiology,
Maratha Mandal’s NGH Institute of Dental Sciences And Research Center, Near K.S.R.P .Ground, R.S.No.47/2, Bauxite Road
Belgaum. (Karnataka), India.
Abstract:
Porphyromonas gingivalis is a major member of red complex in the oral cavity and play a significant role in the progression of adult periodontitis charecterized by alveolar bone loss. Variety of methods such as culture, PCR, Microscopy etc. has been used to detect P.gingivalis but each one has distinct advantages and disadvantages. PCR assays with synthesized 16S r RNA probes enable the detection of virtually any microorganism in a plaque sample. This technique, therefore, is the most sensitive of any of the above.
Aim: To compare culture technique and PCR method to detect and quantify P.gingivalis in subgingival plaque samples in order to assess the relationship of this bacterium with periodontal status.
Materials & Methods: A total of one hundred patients with adult periodontitis and an equal number of controls were selected in an age range of 20 to 50 years and belonged to both the sexes. Sub gingival plaque samples were collected and subjected to anaerobic culture and PCR. In culture species identification was done based on biochemical reactions. The PCR was performed by using specific primers for the 16S rRNA gene of P. gingivalis and quantification was done using standard strain of P.gingivalis.
Results: Comparison was done between the results of anaerobic culture & PCR for control and chronic periodontitis patients. Statistically P value <0.001 is significant & kappa statistics demonstrated moderate agreement (K=0.554) between the above two groups. Results of culture and PCR also depicted that female were prone to chronic periodontitis than male in all the age groups.
Conclusion: The result of PCR confirms those of quantitative culture of P.gingivalis, and offers significant advantages with respect to the rapidity and sensitivity of detection of P.gingivalis in sub gingival plaque samples.
Key Words: Culture; PCR; P.gingivalis; red complex.
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Article citation:-
Varsha Chaudhary, Bhat Kishore, Sunil Rao, Preeti Ingalagi & Manohar Kugaji. Comparison of culture and PCR for detection of Porphyromonas gingivalis in patients with chronic periodontitis and healthy subjects. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 September; 34(34): 1670-1675.Available at http://www.jpbms.info
Copyright © 2013 Varsha Chaudhary, Bhat Kishore, Sunil Rao, Preeti Ingalagi & Manohar Kugaji. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
*Malabika Misra1,Ronita Roychoudhury2,Debjani Sengupta3,Sougata Kumar Burman4,Jayeeta Mukherjee5 & Nayan Chandra Sarkar6
Affiliation:-
1Assistant professor,2,4,5,6Clinical tutor, department of Obstetrics & Gynaecology, Assistant professor, department of community medicine, College of medicine & J.N.M. Hospital, The West Bengal university of health sciences, P.O:- Kalyani, District:- Nadia-741 235,India.
Author’s contributions: - Malabika Misra towards concept, design, literature search, clinical studies, data acquisition, statistical analysis, manuscript writing and editing Ronita Roychoudhury & Debjani Sengupta helps in literature search, data analysis, manuscript editing, and writing. Sougata Kumar Burman, Jayeeta Mukherjee, Nayan Chandra Sarkar analysed, collected the data and helped in manuscript writing.
The name of the Department and Institution to which the work should be attributed:-
College of medicine & J.N.M. Hospital,The West Bengal university of health sciences,P.O:- Kalyani, Distt:- Nadia-741 235,India
Abstract:
Aims: In this study our aim is to find out the best way of delivery of baby in case of caesarean section of obstructed labor cases.
Objectives: Comparing the maternal and fetal outcome of two different methods of delivery of fetus during caesarean section of obstructed labor cases.
Method: This is a comparative study based on clinical observational findings. Following c.s. of obstructed labor cases certain maternal and fetal outcome measures were recorded. Total 122 cases were taken. According to the method of delivery of fetal head these patients were divided into two groups. There were 61 mothers in each group. Their clinical observational records regarding maternal and fetal morbidity were compared and analyzed statistically. In one technique head was delivered first with the help of an extra assistant who pushes fetal head through vagina. In the other, baby was delivered by reverse breech technique.
Result: In the 1st technique laceration of lower flap of uterine incision occurred in 12 cases, whereas in 2nd technique it happened in only 2 cases. Extension of line of incision occurred in 37 cases of caesarean section by the 1st technique. On the other hand, only 9 cases suffered extension of incision line during the delivery of baby by the 2nd technique. These two were statistically significant. Only 7 cases developed mild fever, following c.s. by push technique and 11 cases of the other group developed post-operative fever.
This is insignificant statistically. Mean time of operation in push technique was 55.7 minutes ± 9.05 minutes standard deviation, while in pull technique it is 37 minutes ± 7 minutes standard deviation. Duration of hospital stay, blood loss and these are not different significantly.
Number of foetuses needed NICU admission was significantly higher in the 1st group.
Key Words: Obstructed labor; pull vs. push technique of c.s.
References:
1.Levy R, Chernomoretz T, Appelman Z, Levin D, Or Y, Hagay ZJ.Head pushing versus reverse breech extraction in cases of impacted fetal head during Cesarean section.Eur J Obstet Gynecol Reprod Biol. 2005 Jul 1;121(1):24-6. [Pubmed]
2.Firoozeh Veisi , Maryam Zangeneh , Shohreh Malekkhosravi ,Negin Rezavand. Comparison of “Push” & “Pull”method for impacted fetal head extraction during caesarean delivery International Journal of Gynecology and Obstetrics 2012; 118 (1): 4-6.DOI:10.1016/j.ijgo.2011.12.027[Link]
3.Fasubaa OB, Ezechi OC, Orji EO, Ogunniyi SO, Akindele ST, Loto OM et al Delivery of the impacted head of the fetus at caesarean section after prolonged obstructed labour: a randomised comparative study of two methods J Obstet Gynaecol 2002 22 375–8 doi:10.1080/01443610220141290. [Pubmed][Informa heath care]
Article citation:-
Misra Malabika et al. Comparison of maternal and fetal outcome between two different ways of delivery of impacted fetal head in caesarean section of obstructed labor. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 September; 34(34): 1602-1605.Available at http://www.jpbms.info.
Research article
Firehiwot Cheru, Dessalew Mekonen, Tibebu Girma, Yeshambel Belyhun, Chandrashekhar Unakal*, Mengistu Endris & Feleke Moges
Affiliation:-
School of Biomedical and Laboratory Sciences, University of Gondar, P.O. Box 196, Ethiopia.
Abstract:
Background: Tuberculosis is one of the most common infectious diseases that threaten people living with HIV. It causes a lot of obstacles in diagnosis, and it can significantly affect the course of HIV infection.
Objective: The aim of the study was to compare the treatment outcome of tuberculosis patients in HIV positive and HIV negative patients.
Material and methods:-Between Sep 2002 to Aug 2003 all tuberculosis patients enrolled in DOTS clinic of the University of Gondar Hospital were collected retrospectively from the TB registration book. We compared the data for TB patients of HIV positive and negative subjects. Medical records of the patients were reviewed for age, gender, address, category, and type and treatment outcome.
Results: A total of 1168 tuberculosis patients were registered at Gondar University teaching hospital between September 2002 - August 2003. 637 (54.5%) were urban residents and 531 (45.5%) patient were rural resident. 153 (13.1%) patents were smear positive pulmonary tuberculosis, 678 (58.0%) were smear negative pulmonary tuberculosis and the rest were extra pulmonary tuberculosis 337 (28.9%). We evaluated 1168 registered patients, of these, 385(67%) were HIV positive patients, 190(33%) were HIV negative patients and 593(50.8%) of the total 1168 patients were not tested for HIV. The overall treatment success rate was 294 (59.3%) and failure rate 202 (40.7%) of tuberculosis patients with and without HIV. 87 (50.9%) treatment success was observed in HIV positive patients. Whereas, the treatment success rate was recorded in HIV negative and non-tested TB patients were 44 (45.8%) and 163 (71.2%) respectively
Conclusion and Recommendation: The treatment outcomes of tuberculosis patients with and without HIV in Gondar University Hospital showed the treatment success rate was 59.3%, which is increased compared to previous years.
Key Words: Tuberculosis, Treatment outcome.
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Article citation:-
Firehiwot Cheru et al. Comparison of treatment outcomes of tuberculosis patients with and without HIV in Gondar University Hospital: a retrospective study. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 September; 34(34): 1606-1612. Available at http://www.jpbms.info.
Copyright © 2013 Firehiwot Cheru et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.