DocumentsDate added
Research article
Salah El Din Abdel Hag1 , Sania A. Shaddad2*, Tigani Hassan3, Sumaya I Abass4,
A.K Muddathir5 & Shayoub M. E. A6.
Affiliation:-
1Department of Pharmacology, College of Medicine, University of Bahr Elghazal, Sudan
2Department of Pharmacology, Faculty of Medicine, University of Khartoum, Sudan
3Department of Medicine, Pharmacology & Toxicology, Faculty of Veterinary Medicine University of Khartoum, Sudan
4Microbiology-Veterinary Research Centre, Khartoum Sudan
5Department of Pharmacogonosy, Faculty of Pharmacy, University of Khartoum, Sudan.
6Department of Pharmaceutics, Faculty of Pharmacy, University of Khartoum, Sudan
The name of the Department and Institution to which the work should be attributed:-
Department of Pharmacology,
Department of Medicine,
Microbiology-Veterinary Research Centre,
Department of Pharmacogonosy,University of Khartoum, Sudan.
Department of Pharmacology, College of Medicine, University of Bahr Elghazal, Sudan.
*Corresponding author:
Dr. Sania A. Shaddad.
Department of Pharmacology, Faculty of Medicine, University of Khartoum, Sudan.
Abstract:
Antimicrobial actions of ox bile were tested on pathogenic bacterial isolatess under aseptic conditions using standard methods. These included Staphylococcus albus, Proteus spp., Bacillus Gram +ve spp, Staphylococcus aureus, Klebsiella spp., Pseudomonas aeruginosa, Corynebacterium pseudotuberculosis, Escherichia vulneris, Bacillus subtilis (Chemotherapeutic sensitive), Staphylococcus saprophyticus; Enterobacter spp.; Micrococcus variant; Staphylococcus albus yellow-pigment contaminant; Staphylococcus epidermidis.
Whole ox bile was bacteristatic to all the microorganisms. At the concentration of 33% ox bile was bacteristatic to Coryrebacterium pseudtuberculosis, Micrococcus variant, Staphylococcus albus, and Staphylococcus saprophyticus.
At the concentration of 33% ox bile partially inhibited the growth of Micrococcus luteus, Bacillus subtilis, Enterobacter spp, Staphylococcus epidermidis, Staphylococcus albus, Proteus spp., Bacillus spp., Staphylococcus aureus, Klebsiella spp., Pseudomonas spp, Corynebacterium spp. Escherichia vulneris and Bacillus subtilis were not sensitive to 33% of ox bile.
Key Words: MIC; Ox bile; bactericidal.
REFERENCES
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Article citation:-
Shaddad A. Sania et al. The in vitro inhibitory effect of ox bile on selected bacteria . Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 October;35(35):1767-1773.Available at http://www.jpbms.info
Copyright © 2013 Shaddad A. Sania et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Review article
Nadia Salem & *Azman Abdullah
Affiliation:-
Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur Campus, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
The name of the Department and Institution to which the work should be attributed:-
Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur Campus, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Author contributions
Both the author contributed equally to this paper.
*Corresponding author:
Dr. Azman Abdullah,
Department of Pharmacology,
Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur Campus, Jalan Raja Muda Abdul Aziz,
50300 Kuala Lumpur, Malaysia.
Tel : 006-03-92897508 (Office)
006-016-3185342 (Mobile)
Fax : 006-03-26938205
Abstract:
Carcinogenesis involves multiple steps ranging from the transition of normal to pre-initiated cells to invasive carcinoma. Thus, carcinogenesis provides ample opportunities for chemoprevention. In this context, many naturally-occurring dietary compounds found in fruits and vegetables that are consumed daily have been shown to possess cancer-preventive effects. Sulforaphane (SFN) is a phytochemical compound found in cruciferous vegetables. SFN, a dietary isothiocyanate compound derived from a glucosinolate precursor, has been shown to be a very potent chemopreventive agent in numerous animal carcinogenesis models as well as in cell culture models. SFN exerts its chemopreventive effects by regulating diverse molecular mechanisms. In this review, the molecular mechanisms of SFN which relates to cancer chemoprevention are discussed. These mechanisms include the regulation of phase I and phase II drug metabolizing enzymes. In addition, the influence exerted by sulforaphane upon signaling pathways relating to apoptosis, cell cycle arrest and transcription factors Nrf2, NF-κB and AP-1 are also discussed.
Key Words: Cancer chemoprevention; sulforaphane; phase I drug metabolizing enzymes; phase II drug metabolizing enzymes; apoptosis; cell cycle arrest; histone deacetylase; Nrf2, NF-κB; AP-1, COX-2.
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Article citation:-
Salem Nadia & Abdullah Azman. Sulforaphane and its function in cancer chemoprevention. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 October; 35(35):1788-1795.Available at http://www.jpbms.info
Copyright © 2013 Salem Nadia & Abdullah Azman. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
0riginal article
Kuldeep Kumar1*, Sandeep Punia2, Jagjit Kaur 3 and Teena Pathak 4
Affiliation:-
1Assistant Professor, 2,4Ph. D. Students, 3Research Scholar, Department of Biotechnology, M. M. Modi College, Patiala-147 001 Punjab (India)
The name of the Department and Institution to which the work should be attributed:-
Department of Biotechnology,M. M. Modi College, Patiala-147 001 Punjab (India).
Authors contributions:
Author 1 contributes towards the Concepts, Design, Literature search, Clinical studies, Experimental studies, Data acquisition, Data analysis, Manuscript preparation, editing and review. Author 2 & 4 worked in literature search, experimental studies, data acquisition and manuscript preparation. Author 3 contributes towards literature search and manuscript preparation.
*Corresponding author:
Dr. Kuldeep Kumar.
Assiatant Professor, Department of Biotechnology
Mulatni Mal Modi College, Patiala 147001, Punjab (India)
Abstract:
Aim: L-asparaginase is an enzyme of great therapeutic value and is used worldwide. Citrus limon is used for this purpose as it is a potential source of L-asparaginase. This work has been carried out to develop plant asparaginase based asparagine biosensor for leukemia. It is a novel and diagnostic biosensor for monitoring asparagine levels in leukemic patients.
Methods: Various immobilization strategies have been applied to fabricate the biosensor and improve the stability of L-asparaginase. Phenol red indicator has been coimmobilized with asparaginase from Citrus limon and color visualization approach has been optimized for varying concentrations of asparagine.
Results and conclusion: The detection limit of asparagine achieved with different immobilization techniques such as agar method, agarose method and gelatin method is 10-1 –10-10M. Immobilization with agar method is found to be more effective due to faster response time. Furthermore these immobilization techniques have been applied for the detection of asparagine in normal and leukemia serum samples.
Key Words: Biosensor; Immobilized; L-asparaginase; Leukemia.
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Article citation:-
Kumar Kuldeep, Punia Sandeep, Kaur Jagjit, Pathak Teena. Development of plant asparagine biosensor for detection of leukemia. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 October; 35(35):1796-1801.Available at http://www.jpbms.info
Original article
Debasis Das1,Kanti Bhushan Choudhury2,Sita Chattapadhyay3, Sougata Kumar Burman*4,Maitreyi Bandyopadhyay5, Abhijit Bhakta6
Affiliation:-
1Associate Professor, Community Medicine, Malda Medical College, Malda, West bengal,India.
2Assistant Professor, Community Medicine, Calcutta National Medical College, Kolkata,India.
3Associate Professor, Community Medicine, I.P.G.M.E.& R, Kolkata, India
4Clinical Tutor, Department of Obstetrics & Gynaecology, College of Medicine & J N M Hospital, WBUHS, Kalyani, Nadia, India.
5Assistant Professor, Department of Microbiology, R G Kar Medical College, Kolkata,India
6Associate Professor, Department of Anatomy R S Medical College, Kolkata, India
The name of the Department and Institution to which the work should be attributed:-
I C T C Centre
I P G M E & R
Kolkata
Author contributions:
All the authors equally to this paper.
*Correspondence to:
Dr Sougata Kumar Burman
Clinical Tutor
Obstetrics & Gynaecology.
College of Medicine & J N M Hospital,WBUHS.
Kalyni, West Bengal,.India.
Mobile: 09475943811
Abstract:
Objective: Recording the profile & queries of the clients attending Integrated Counselling & Testing Centre so that this information can be used for developing preparedness of the counsellor to handle questions of the clients in clinic. Design: It is a type of cross-sectional need assessment study. Setting: Counselling centre set-up of Institute of Post Graduate Medical Education & Research, Kolkata, West Bengal. Method: All the 259 clients attending the ICTC centre during March – May’2011 were listened passively during group counselling followed by collection of selected client variable from the client records were done. Results: Among total 259 clients 36.68% were rural residents, others semi-urban or urban dwellers, 82.63% came referred from antenatal clinic & 13.51% from obstetrics & gynaecology clinic, mostly female, 97.37% in reproductive age group, mean age being 28.11±18.18 years, 7.45% illiterate & 97.3% married, 97.68% heterosexual, others being student and/or unmarried. No other risk factor like homosexuality, history of blood transfusion, history of use of infected syringe and needle in health facility found among any client. All clients are counselled, found HIV negative, 2.7% spouse tested. There are 75 different types of questions asked by 195 clients on general aspect of HIV /AIDS, transmission, prevention, treatment, tests and some unrelated areas. Among those who asked questions, 90.77percent able to comprehend the answer given by the counsellor, 58.97% found satisfied. Conclusion: To develop preparedness of the counsellor in ICTC, recording client queries is valuable.
Key words: Client, Queries; ICTC; India.
REFERENCES:
1.Operational Guidelines for Integrated Counselling and Testing Centres: National AIDS Control Organization. Ministry of Health & Family Welfare, Government of India, July 2007.
2.Olanrewjiu A M, Ola F A, Akintunde A E, Ibrahim B & Ibiyemi F. HIV voluntary counselling and testing of pregnant women in primary health care centres in Ilesa, Nigeria. The Internet Journal of Third World Medicine, 2007. Vol. 6 No. 1.
3.Pool R, Nyanzi S, Whitworth J A. Attitudes to voluntary counselling and testing for HIV among pregnant women in rural south-west Uganda. AIDS Care, 2001Oct; 13(5):605-15.
Article citation:-
Das Debasis et al. Queries of clients in integrated counselling & testing centre of a tertiary care hospital of West Bengal, India. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 October; 35(35): 1826-1829. Available at http: //www.jpbms.info.
Copyright © 2013 Das Debasis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Soni Hardik K1*, Patel Vrushali V2, Vaghasiya Jitendra D2,
Patel Vandana B2, Patel Ghanshyam R1
Affiliation:-
1Vasu Research Centre, A Division of Vasu Healthcare Pvt. Ltd., 896/A, G.I.D.C., Makarpura, Vadodara – 390 010, Gujarat, India.
2Department of Pharmacology, Babaria Institute of Pharmacy, BITS Edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara – 391 240, Gujarat, India.
The name of the Department and Institution to which the work should be attributed:-
Vasu Research Centre, A Division of Vasu Healthcare Pvt. Ltd., 896/A, G.I.D.C., Makarpura, Vadodara – 390 010, Gujarat, India
Author contributions:
Soni Hardik K: Concept, design, literature search, data analysis and manuscript preparation.
Patel Vrushali V: Literature search, Experimental study, Data acquisition and Statistical analysis.
Vaghasiya Jitendra D and Patel Vandana B: Data analysis and manuscript review.
Patel Ghanshyam R: Data analysis and manuscript editing.
Core Idea: The study was initiated to evaluate safety and efficacy of anti-urolithiatic formulation developed from herbo-mineral source. Major ingredients of UCEX01 are individually well reported in Ayurvedic texts and scientific research publications for variety of activities like diuretic, anti-urolithiatic, anti-inflammatory etc. However, no such evidence was available which proves the safety and efficacy of such combination. Therefore, present study was taken up to evaluate acute toxicity and anti-urolithiatic activity of UCEX01.
*Correspondence to:
Soni Hardik K
Asst. Manager, R&D
Vasu Research Centre (A Division of Vasu Healthcare Pvt. Ltd.)
896/A, G.I.D.C., Makarpura, Vadodara-390010, Gujarat, India.
Tel.: 91-265-2657701, 2657702, Fax: 91-265-2647331
Mob.: 91-9428692240.
Abstract:
Urolithiasis is the most common urinary tract disorder with high recurrence. But, unfortunately most of its treatments are expensive or having side effects. Therefore, the search for anti-urolithiatic drugs from natural sources has been of great importance. UCEX01 is the herbo-mineral Ayurvedic formulation used for the treatment of kidney stone. But, no scientific evidences are available which proves safety and efficacy of such combination. Hence, present study was conducted to evaluate acute toxicity and anti-urolithiatic activity of UCEX01. Healthy male Wistar rats were used by dividing randomly into 4 groups. Group I was considered as normal control. Group II was as Disease control, Group III as UCEX01 treated with Therapeutic Effective Dose (TED-I) and Group IV was as UCEX01 treated with double of Therapeutic Effective Dose (TED-II). After 28th day, blood and urine sample were collected. Urine volume and pH was measured immediately. Bio-chemical parameters like calcium, phosphorus, creatinine and uric acid were estimated in serum and urine. Histopathology of kidney was also carried out. No mortality was observed during acute oral toxicity study. On basis of study data it can be concluded that the treatment of UCEX01 has significant anti-urolithiatic effect on ethylene glycol induced urolithiasis in rats. The underlying mechanism(s) of this effect is unknown however it may be attributed to its diuretic, anti-inflammatory and lowering of urinary concentrations of stone-forming elements. It can be a safe and effective remedy for the treatment of kidney stone.
Key words: UCEX01; ethylene glycol; urolithiasis; herbo-mineral Ayurvedic formulation.
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Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: Nil
Article citation:-
Soni Hardik K, Patel Vrushali V, Vaghasiya Jitendra D, Patel Vandana B, Patel Ghanshyam R. Pharmacological evaluation of Anti-urolithiatic activity of UCEX01 - A herbo-mineral ayurvedic formulation. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 October; 35(35): 1834-1839. Available at http: //www.jpbms.info.
Copyright © 2013 Soni Hardik K, Patel Vrushali V, Vaghasiya Jitendra D, Patel Vandana B, Patel Ghanshyam R. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.