DocumentsDate added
Original article
Bhoomi B. Joshi, Megha G. Chaudhari and Kinnari N. Mistry*
Affiliation:-
Ashok & Rita Patel Institute of Integrated Studies in Biotechnology & Allied Sciences (ARIBAS), New Vallabh Vidhya Nagar – 388121, (Gujarat) India
Author’s contributions: - All the author contributed equally to this paper.
The name of the department(s) and institution(s) to which the work should be attributed:
Ashok & Rita Patel Institute of Integrated Studies in Biotechnology & Allied Sciences (ARIBAS).
*Corresponding author:-
Kinnari N. Mistry
Associate professor in biochemistry, Ashok & Rita Patel Institute of Integrated Studies in Biotechnology & Allied Sciences (ARIBAS), New Vallabh Vidhya Nagar – 388121, (Gujarat) India
Contact no:- +91-9825857880
Abstract:
The objective of present work was to evaluate the antidiabetic and anti-inflammatory activity from methanolic, ethanolic and chloroform crude extract of Argyreia nervosa roots. In vitro anti-inflammatory activity was carried out by inhibiting the heat induced albumin denaturation, membrane stabilization and protein denaturation activity. The samples were studied for their effect on inhibition of glycosylation of haemoglobin, glucose transport across yeast cells and α- Amylase inhibition. From the results of the study, it is inferred that A.nervosa root possesses good anti-inflammatory and anti-diabetic activity. Moreover the results also confirmed that the methanol proved to be superior type solvent in compare to ethanol and chloroform to carry out crude extraction procedure of A.nervosa root. This activity may be due to the strong occurance of phenolic compounds such as alkaloids, flavanoids, tannins, steroids and phenols. However, these effects need to be confirmed using in vivo models and clinical trials for its effective utilization as therapeutic agents.
Key words: Argyreia nervosa, In-vitro Antidiabetic and anti-inflammatory assay, Metronidazole, Aspirin.
REFERENCES
1.Agarwal SR, Rastogi RP. Pharmacognostical and Preliminary Phytochemical Studies of Argyreia nervosa Burm. Indian J Pharmacol.1974, 35:118-119.
2.Ali, H., P.J.Houghton, and Soumyanath. Recent advance to evaluate antidainetic drugs. A. J. Ethnopharmacol.2006, 107: 449-455.
3.Fluickiger, R., and K. H. Winterhalter. Glycosylated hemoglobins. In Biochemical and Clinical Aspects of Hemoglobin Abnormalities. W. S. Caughey, editor.Academic Press, Inc.1978,205-214.
4.B. Dinesh Kumar, A. Mitra and M. Manjunatha. Invitro and invivo studies of Antidiabetic Indian medicinal plants:A Review. Journal of Herbal Medicine and Toxicology. 2009, 3 (2), 9-14.
5.Syamsudin,S. Standardization of extract of Leucaena leucocephala (lmk) De Wit seeds by -glucosidase inhibitor. Int. J. Phytomedicine. 2010, 2. 430-435.
6.García-Lafuente A, Guillamón E, Villares A, Rostagno MA and Martínez JA. Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflamm. Res. 2009, 58: 537-552.
7.Gonen, B., A. H. Rubenstein, H. Rochman, S. P. Tanega, and D. L. Horwitz,. Haemoglobin Al. An indication of the metabolic control of diabetic patients. 1977, 2(2) 734-737.
8.Prachayasittikul S, Buraparuangsang P, Worachartcheewan A, Isarankura-Na-Ayudhya C, Ruchirawat S, Prachayasittikul V . Antimicrobial and antioxidant activity of bioreactive constituents from Hydnophytum formicarum Jack Molecular. 2008, 13: 904-921.
9.Rajiv Gandhi, G., and Sasikumar, P. Antidiabetic effect of Merremia emarginata Burm.F. In Streptozotocin induced diabetic rats. Asian. Paci. J. Tropi. Biomedicine. 2012, 2: 281-286.
10.Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziwa P, Nkeh-Chungag BN, et al. An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J Med Plants Res. 2011, 4: 789-795
11.Tanzer, M. L., R. Fairweather, and P. M. Gallop. Collagen crosslinks: isolation of reduced NEhexosyl- hydroxylysine from borohydride reduced calf skin insoluble collagen. Arch. Biochemn. Biophys. 1972, 151: 137- 141.
12.Arulmozhi S, Papiya MM, Purnima A and Sathiya N. In Vitro Antioxidant and Free Radical Scavenging Activity of Alstonia scholaris Linn. R.Br. Iranian Journal of Pharmacology and Therapeutics. 2008, 6: 191-196.
13.Sankari G, Mounnissamy VM, and Balu V, Evaluation of anti-inflammatory and membrane stabilizing properties of ethanolic extracts of Diptheracanthus prostatus(Acanthaceae), Amala Research Bulletin. 2009, 29:188-89.
14.Zoccoli, M. A., S. A. Baldwin, and G. E. Lienhard. The monosaccharide transport system of the human erythrocyte.J. Biol. Chem. 2004, 253: 6923-6930
15.Sankari G, Mounnissamy VM, and Balu V. Evaluation of anti-inflammatory and membrane stabilizing properties of ethanolic extracts of Diptheracanthus prostatus(Acanthaceae), Amala Research Bulletin. 2009, 29:188-89.
16.VP Cirillo. Mechanism of Arabinose transport in Tetrahymena pyriformis.J Bacteriol.1962, 84, 485–491.
17.Gupta Daksha, Chandrashekar, Richard Lobo, Yogendra Gupta Nilesh. In-vitro Antidiabetic activity of stem bark of Buhinia purpuria Linn. 2012,4(2):614-619.
18.Conforti F, Statti G, Loizzo MR, Sacchetti G, Poli F, Menichini F.Biological & Pharmaceutical Bulletin. 2005, 28 (6): 1098-1102.
19.Gabbay, K. H., J. M. Sosenko, G. A. Banuchi, M. J. Mininsohn, and R. Fliuckiger. Glycosylated hemoglobins: increased glycosylation of hemoglobin A in diabetic patients. 1976, 28: 337-340.
20.Horton, B. F., and T. H. J. Huisman. Studies on the heterogeneity of hemoglobin. VII minor haemoglobin components in haematological diseases. Br. J. Haematol. 1965, 11: 296-304.
21.Bunn, H. F., D. N. Haney, K. H. Gabbay, and P. M. Gallop. Further identification of the nature and linkage of the carbohydrate in hemoglobin Alc. Biocheml. Biophys. Res. Cornm) 7unt. 1975, 67: 103-109.
22.Cerami, A., R. Koenig, and C. M. Peterson. Haemoglobin A1, and diabetes mellitus. Br. J. Haeematol. 1978, 38: 1-4
23.S. Umadevi, G. P. Mohanta, V. Chelledurai, P. K. Manavalan. Antibacterial and antifungal activity of Andrographis echiodes. Journal of Natural Remedies. 2003. 3(2):185-188
24.Govindappa M. , Naga Sravya S., Poojashri M. N., Sadananda T. S. and Chandrappa C. P. Antimicrobial, antioxidant and in vitro anti-inflammatory activity of ethanol extract and active phytochemical screening of Wedelia trilobata (L.) Hitchc. Journal of Pharmacognosy and Phytotherapy. 2011, 3(3):43-51.
25.Cerami, A., R. Koenig, and C. M. Peterson. Haemoglobin A1, and diabetes mellitus. Br. J. Haeematol. 1978, 38: 1-4.
26.Chaitanya R, Sandhya S, David Banji, Vinod K.R and Murali.S HRBC Membrane Stabilizing Property of Root, Stem and Leaf of Glochidion velutinum International Journal of Research in Pharmaceutical and Biomedical Sciences. 2011, 2 (1): 2229-3701.
27.Turker AU, Usta C. Biological screening of some Turkish medicinal plants for antimicrobial and toxicity studies. Nat. Prod. 2008, 22: 136-146.
28.Duraipandiyan, V., Ayyanar, M. and Ignacimuthu, S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complementary Altern. Med., 2006, 6: 35-41.
29.G. Prakash Yoganandam1, K.Ilango, Sucharita De. Evaluation of Anti-inflammatory and Membrane Stabilizing Properties of various extracts of Punica granatum L.(Lythraceae) International Journal of PharmTech Research CODEN. 2010, 2(2): 1260-1263.
30.Williams L.A.D., O’Connar A., Latore L., Dennis O., Ringer S., Whittaker J.A., Conrad J., Vogler B., Rosner H., Kraus W. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process. West Indian Med. J. 2008, 57: 327-331.
31.Mizushima Y, Kobayashi M. Interaction of anti ‐inflammatory drugs with serum proteins, especially with some biologically active proteins. J. Pharm. Pharm., 1968, 20: 169-173.
32.Manohara K.P., Raveendra Reddy P., Nandeesh R., Vijay kumar S. Evaluation of Anti- inflammatory activity of various extracts of Tagetes erecta Linn. Herbal Heritage., 2009, 1(2),58-63.
33.Bacchav AS, Gulache VS, Upasain CD. Analgesic and Anti Inflammatory activity of Argyreia nervosa Root. Indian J Phamacol., 2009, 41(4):158-161
34.Bailey, A. J., S. D. Robins, M. J. A. Tanner. Reducible components in the proteins of human erythrocyte membrane. Biochim. Biophys. Acta. 2009, 434: 51-57.
35.Subramonium A, Madhavachandran V, Ravi K, Anuja VS. Aphrodisiac property of the Argyreia nervosa Elephant Creeper- J Endocrinol Report. 2007, 11(2):82-85.
36.Gokhle AB, Damre AS, Saraf MN. Investigation in to the Immunomodulatory activity of Argyreia speciosa- J Ethanopharmacol. 2003, 84(1):109-114.
37.Opie EL. On the relation of necrosis and inflammation to denaturation of proteins. J Exp Med. 1968, 115: 597-608.
38.VP Cirillo. Mechanism of Arabinose transport in Tetrahymena pyriformis.J Bacteriol.1962, 84, 485–491.
39.Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. I. J. Pharm. Pharm. Sci. 2010, 2(1): 146-155.
40.Shapiro, R., M. J. McManus, C. Zalut, and H. F. Bunn. Sites of non-enzymatic glycosylation of human hemoglobin A. J. Biol. Chemi.1980, 1(2) 165-178.
41.Shaw Cross WE. Recreational use of ergoline alkaloids from Argyreia nervosa. Journal Psychoactive drugs. 1983, 15(4): 251-259.
42.Koenig, R. J., C. M. Peterson, R. L. Jones, C. Saudek, M. Lehrman, and A. Cerami. Correlation of glucose regulation and hemoglobin A, in diabetes mellitus. N. Enigl. J. M11ed. 1976, 295: 417-420.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: Nil
Article citation:-
Rajyalakshmi Gunti,Usha Rani Anaparthy,Durga Rani Arava. In vitro screening of anti-inflammatory and anti-diabetic activity of root extract of Argyreia nervosa. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 December 37(37): 1964-1971. Available at www.jpbms.info.
Copyright © 2013 Bhoomi B. Joshi, Megha G. Chaudhari,Kinnari N. Mistry. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
Sougata Kumar Burman1,*, Arnab Kumar Mandal2, Debasis Das3, Jayati Das4
Affiliation:-
1Clinical tutot, Department of Obstetrics & Gynaecology, College of Medicine & J N M Hospital, WBUHS, Kalyani, West Bengal,India.
2Assistant Professor, Microbiology, Malda Medical College & Hospital, Malda, West Bengal, India.
3Associate Professor, Community Medicine, Microbiology, Malda Medical College & Hospital, Malda, West Bengal,India.
4Assistant Professor, Physiology, Institute of Post Graduate Medical Education & Research, Kolkata, West Bengal, India.
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Obstetrics & Gynaecology,
College of Medicine & J N M Hospital, WBUHS, Kalyani, Nadia, India.
*Corresponding author:-
Dr. Sougata Kumar Burman
Clinical tutor, Department of Obstetrics & Gynaecology, College of Medicine & J N M Hospital, WBUHS, Kalyani, Nadia,India.
Contact number: +91-9475943811
Abstract:
Introduction: The cases of mucocutaneous candidiasis have increased dramatically in past twenty years, especially associated with incremental incidences of immunocompromised patients. It is necessary to evaluate the existing anti-fungal susceptibility pattern due to emergence of resistant strains. Objective: The objective of this study is to identify the fungal pathogens from cases of mucocutaneous candidiasis and to establish the anti-fungal susceptibility pattern of the isolated species. Materials & methods: A total of 120 clinically suspected cases of mucocutaneous candidiasis identified from patients attending in different out patient departments and indoor wards of a tertiary care hospital, Kolkata, India, over one year. Different candida species were isolated by microscopy, culture & biochemical reactions. Anti-fungal susceptibility testing of isolates was done by Kirby-Bauer disc diffusion method using anti-fungal drugs Fluconazole, Itraconazole & Voriconazole.
Results: Candida albicans were isolated in 92 (76.6%) cases, Candida tropicalis, Candida krusei, Candida parapsilosis were isolated in 14 (11.7%), 8(6.7%) and 6(5%) cases respectively. Anti-fungal susceptibility pattern showed Fluconazole was sensitive against 84(91.30%) isolated strains of Candida albicans, 8(57.14%) strains of Candida tropicalis, 50% strains of Candida parapsilosis & resistant against all strains of Candida krusei but Voriconazole & Itraconazole were sensitive against all isolated Candida species.
Conclusions: Study showed the emergence of Fluconazole resistant pathogenic Candida species. Voriconazole & Itraconazole were very effective against all isolated candida strains including Fluconazole resistant ones.
Key words: Mucocuteneous Candidiasis; Causative species; anti-fungal susceptibility; Tertiary Care Hospital; India.
REFERENCES
1.Agarwal SR, Rastogi RP. Pharmacognostical and Preliminary Phytochemical Studies of Argyreia nervosa Burm. Indian J Pharmacol.1974, 35:118-119.
2.Ali, H., P.J.Houghton, and Soumyanath. Recent advance to evaluate antidainetic drugs. A. J. Ethnopharmacol.2006, 107: 449-455.
3.Fluickiger, R., and K. H. Winterhalter. Glycosylated hemoglobins. In Biochemical and Clinical Aspects of Hemoglobin Abnormalities. W. S. Caughey, editor.Academic Press, Inc.1978,205-214.
4.B. Dinesh Kumar, A. Mitra and M. Manjunatha. Invitro and invivo studies of Antidiabetic Indian medicinal plants:A Review. Journal of Herbal Medicine and Toxicology. 2009, 3 (2), 9-14.
5.Syamsudin,S. Standardization of extract of Leucaena leucocephala (lmk) De Wit seeds by -glucosidase inhibitor. Int. J. Phytomedicine. 2010, 2. 430-435.
6.García-Lafuente A, Guillamón E, Villares A, Rostagno MA and Martínez JA. Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflamm. Res. 2009, 58: 537-552.
7.Gonen, B., A. H. Rubenstein, H. Rochman, S. P. Tanega, and D. L. Horwitz,. Haemoglobin Al. An indication of the metabolic control of diabetic patients. 1977, 2(2) 734-737.
8.Prachayasittikul S, Buraparuangsang P, Worachartcheewan A, Isarankura-Na-Ayudhya C, Ruchirawat S, Prachayasittikul V . Antimicrobial and antioxidant activity of bioreactive constituents from Hydnophytum formicarum Jack Molecular. 2008, 13: 904-921.
9.Rajiv Gandhi, G., and Sasikumar, P. Antidiabetic effect of Merremia emarginata Burm.F. In Streptozotocin induced diabetic rats. Asian. Paci. J. Tropi. Biomedicine. 2012, 2: 281-286.
10.Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziwa P, Nkeh-Chungag BN, et al. An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J Med Plants Res. 2011, 4: 789-795
11.Tanzer, M. L., R. Fairweather, and P. M. Gallop. Collagen crosslinks: isolation of reduced NEhexosyl- hydroxylysine from borohydride reduced calf skin insoluble collagen. Arch. Biochemn. Biophys. 1972, 151: 137- 141.
12.Arulmozhi S, Papiya MM, Purnima A and Sathiya N. In Vitro Antioxidant and Free Radical Scavenging Activity of Alstonia scholaris Linn. R.Br. Iranian Journal of Pharmacology and Therapeutics. 2008, 6: 191-196.
13.Sankari G, Mounnissamy VM, and Balu V, Evaluation of anti-inflammatory and membrane stabilizing properties of ethanolic extracts of Diptheracanthus prostatus(Acanthaceae), Amala Research Bulletin. 2009, 29:188-89.
14.Zoccoli, M. A., S. A. Baldwin, and G. E. Lienhard. The monosaccharide transport system of the human erythrocyte.J. Biol. Chem. 2004, 253: 6923-6930
15.Sankari G, Mounnissamy VM, and Balu V. Evaluation of anti-inflammatory and membrane stabilizing properties of ethanolic extracts of Diptheracanthus prostatus(Acanthaceae), Amala Research Bulletin. 2009, 29:188-89.
16.VP Cirillo. Mechanism of Arabinose transport in Tetrahymena pyriformis.J Bacteriol.1962, 84, 485–491.
17.Gupta Daksha, Chandrashekar, Richard Lobo, Yogendra Gupta Nilesh. In-vitro Antidiabetic activity of stem bark of Buhinia purpuria Linn. 2012,4(2):614-619.
18.Conforti F, Statti G, Loizzo MR, Sacchetti G, Poli F, Menichini F.Biological & Pharmaceutical Bulletin. 2005, 28 (6): 1098-1102.
19.Gabbay, K. H., J. M. Sosenko, G. A. Banuchi, M. J. Mininsohn, and R. Fliuckiger. Glycosylated hemoglobins: increased glycosylation of hemoglobin A in diabetic patients. 1976, 28: 337-340.
20.Horton, B. F., and T. H. J. Huisman. Studies on the heterogeneity of hemoglobin. VII minor haemoglobin components in haematological diseases. Br. J. Haematol. 1965, 11: 296-304.
21.Bunn, H. F., D. N. Haney, K. H. Gabbay, and P. M. Gallop. Further identification of the nature and linkage of the carbohydrate in hemoglobin Alc. Biocheml. Biophys. Res. Cornm) 7unt. 1975, 67: 103-109.
22.Cerami, A., R. Koenig, and C. M. Peterson. Haemoglobin A1, and diabetes mellitus. Br. J. Haeematol. 1978, 38: 1-4
23.S. Umadevi, G. P. Mohanta, V. Chelledurai, P. K. Manavalan. Antibacterial and antifungal activity of Andrographis echiodes. Journal of Natural Remedies. 2003. 3(2):185-188
24.Govindappa M. , Naga Sravya S., Poojashri M. N., Sadananda T. S. and Chandrappa C. P. Antimicrobial, antioxidant and in vitro anti-inflammatory activity of ethanol extract and active phytochemical screening of Wedelia trilobata (L.) Hitchc. Journal of Pharmacognosy and Phytotherapy. 2011, 3(3):43-51.
25.Cerami, A., R. Koenig, and C. M. Peterson. Haemoglobin A1, and diabetes mellitus. Br. J. Haeematol. 1978, 38: 1-4.
26.Chaitanya R, Sandhya S, David Banji, Vinod K.R and Murali.S HRBC Membrane Stabilizing Property of Root, Stem and Leaf of Glochidion velutinum International Journal of Research in Pharmaceutical and Biomedical Sciences. 2011, 2 (1): 2229-3701.
27.Turker AU, Usta C. Biological screening of some Turkish medicinal plants for antimicrobial and toxicity studies. Nat. Prod. 2008, 22: 136-146.
28.Duraipandiyan, V., Ayyanar, M. and Ignacimuthu, S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complementary Altern. Med., 2006, 6: 35-41.
29.G. Prakash Yoganandam1, K.Ilango, Sucharita De. Evaluation of Anti-inflammatory and Membrane Stabilizing Properties of various extracts of Punica granatum L.(Lythraceae) International Journal of PharmTech Research CODEN. 2010, 2(2): 1260-1263.
30.Williams L.A.D., O’Connar A., Latore L., Dennis O., Ringer S., Whittaker J.A., Conrad J., Vogler B., Rosner H., Kraus W. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process. West Indian Med. J. 2008, 57: 327-331.
31.Mizushima Y, Kobayashi M. Interaction of anti ‐inflammatory drugs with serum proteins, especially with some biologically active proteins. J. Pharm. Pharm., 1968, 20: 169-173.
32.Manohara K.P., Raveendra Reddy P., Nandeesh R., Vijay kumar S. Evaluation of Anti- inflammatory activity of various extracts of Tagetes erecta Linn. Herbal Heritage., 2009, 1(2),58-63.
33.Bacchav AS, Gulache VS, Upasain CD. Analgesic and Anti Inflammatory activity of Argyreia nervosa Root. Indian J Phamacol., 2009, 41(4):158-161
34.Bailey, A. J., S. D. Robins, M. J. A. Tanner. Reducible components in the proteins of human erythrocyte membrane. Biochim. Biophys. Acta. 2009, 434: 51-57.
35.Subramonium A, Madhavachandran V, Ravi K, Anuja VS. Aphrodisiac property of the Argyreia nervosa Elephant Creeper- J Endocrinol Report. 2007, 11(2):82-85.
36.Gokhle AB, Damre AS, Saraf MN. Investigation in to the Immunomodulatory activity of Argyreia speciosa- J Ethanopharmacol. 2003, 84(1):109-114.
37.Opie EL. On the relation of necrosis and inflammation to denaturation of proteins. J Exp Med. 1968, 115: 597-608.
38.VP Cirillo. Mechanism of Arabinose transport in Tetrahymena pyriformis.J Bacteriol.1962, 84, 485–491.
39.Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. I. J. Pharm. Pharm. Sci. 2010, 2(1): 146-155.
40.Shapiro, R., M. J. McManus, C. Zalut, and H. F. Bunn. Sites of non-enzymatic glycosylation of human hemoglobin A. J. Biol. Chemi.1980, 1(2) 165-178.
41.Shaw Cross WE. Recreational use of ergoline alkaloids from Argyreia nervosa. Journal Psychoactive drugs. 1983, 15(4): 251-259.
42.Koenig, R. J., C. M. Peterson, R. L. Jones, C. Saudek, M. Lehrman, and A. Cerami. Correlation of glucose regulation and hemoglobin A, in diabetes mellitus. N. Enigl. J. M11ed. 1976, 295: 417-420.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: Nil
Copyright © 2013 Bhoomi B. Joshi, Megha G. Chaudhari,Kinnari N. Mistry. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Debasis Das1, Sougata Kumar Burman2,*,Goutam Dhar3,Devjyoti Santra4,Prasantha Kumar Das5, Projjwal Sengupta6
Affiliation:-
1Associate Professor, Deaprtment of PSM , Malda Medical College, Malda, West bengal,India
2Clinical Tutor, Department of Obstetrics & Gynaecology, College of Medicine & J N M Hospital, WBUHS, Kalyani, Nadia, India
3Associate Professor, Community Medicine, ADME, Swasthya Bhavan, Kolkata,India
4Associate Professor, Department of Obstetrics & Gynaecology, B S Medical College, Bankura,West Bengal,India
5Associate Professor, Department of Psychiatry medicine,Medical college,Kolkata,India
6Assistant Professor, Community mediciane,NRS medical college,Kolkata,India
The name of the Department and Institution to which the work should be attributed:-
Deaprtment of PSM, Malda Medical College, Malda, West bengal, India
Department of Obstetrics & Gynaecology, College of Medicine & J N M Hospital, WBUHS, Kalyani, Nadia, India
B S Medical College,Bankura,West Bengal,India
ADME, Swasthya Bhavan, Kolkata,India
NRS medical college,Kolkata,India
*Correspondence to:
Dr Sougata Kumar Burman.
Clinical Tutor,
Obstetrics & Gynaecology. College of Medicine & J N M Hospital,WBUHS. Kalyni, West Bengal,India.
Mobile: 09475943811
Abstract:
Background: Team concept and leadership capacity probably played the most important role in functioning of any organization. It is also true for modern health care organization. Very few studies assessed leadership behaviour of doctors. Methodology: It is a cross-sectional, behaviour assessment study, conducted between February – July 2009 involving 50% doctors selected by stratified random sampling technique of N.R.S.Medical College and K.P.C.Medical College, Kolkata. Leadership behaviour assessed following Managerial Grid Model of Robert Blake and Jane Mouton.
Results: 122 doctors in N.R.S Medical College and 53 doctors in K.P.C Medical College were included in the study. In both the institutions ‘team’ type leaders dominate (71.7% in K.P.C Medical College and 62.6% in N.R.S Medical College). Statistically no significant relationship between leadership behaviour and age, gender, duration of experience, academic qualification, management qualification, hierarchical position, and work experience in different organization and experiences of holding administrative post by the doctors were found in either institution & significant relationship between leadership behaviour and discipline was found in N.R.S. Medical College. Conclusion: A dispersed leadership pattern was found where most desirable leadership type i.e., ‘Team’ type of leaders were mostly prevalent in both the Institutions. Considerable scope for improvement was there in each of Task & Relationship domain of leadership behaviour.
Key words: Leadership behavior; Teacher-doctor; Government & Private Medical College; India.
Article citation:-
Das Debasis et al. How doctors lead? – A comparative study on leadership behaviour in a Government & a Private Hospital of Kolkata, India. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 December; 37(37): 1948-1951. Available at http: //www.jpbms.info.
REFERENCES
1.Chaudry J, Jain A, McKenzie S, Schwartz WR. Physician Leadership: The Competencies of Change. Journal of Surgical Research. 2008;148(1): 49-59.
2.Crofts L. Leadership programme for critical care. Radiography. 2006; 12(4): 305-313.
3.Morgeson P, Frederick,DeRue D, Scott. Event criticality, urgency and duration: Understanding how events disrupt teams and influence team leader intervention. Intensive & Critical Care Nursing. 2008; 22(4): 220-227.
4.Reeleder D, Goel V, Singer A, Peter & Douglas K, Martin. Leadership and priority setting: The perspective of hospital CEOs. Journal of Surgical Education. 2008; 65(3): 213-220.
5.Wiley W, Souba. The Inward Journey of Leadership. The Leadership Quarterly. 2006; 17(3): 271-287.
6.www.leadership-and-motivation-training.com; visited on 03/06/2013.
7.www.gridinternational.com; visited on 03/06/2013.
8.Yielder J. Leadership and power in medical imaging. Health Policy. 2006; 79(1): 24-34.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: Nil
Copyright © 2013 Das Debasis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Case report
Manisa Sahu1,*, Arun Bal2, Pallavi Bhalekar3, Dipty Kenny4
Affiliation:-
1Consultant Microbiologist,2Consultant Diabetic surgeon, 3Technical Supervisor, 4Technologist, S L Raheja Hospital (A Fortis Associate), Mahim (W), Mumbai-400016,India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology and Department of Diabetic Foot Surgery: S L Raheja Hospital (A Fortis Associate), Mahim (W), Mumbai-400016,India
*Corresponding author:-
Dr. Manisa Sahu. MD, DNB (Micro);
Consultant Microbiologist, S L Raheja Hospital (A Fortis Associate), Mahim (W), Mumbai-400016,India
Abstract:
Diabetic foot infections are usually polymicrobial, including fungal pathogens. We Report a case of DFI in a 53 year old female patient due to Fusarium species. Fusarium species, a hyaline mold, was isolated on two successive occasions. No systemic dissemination was noted and patient was managed by extensive debridement of the ulcer.
Key words: DFI; Diabetic foot infections; Fusarium species.
REFERENCES
1.Shalbha Tiwari, Daliparthy D. Pratyush, Awanindra Dwivedi, Sanjiv K. Gupta2,Madhukar Rai, Surya K. Singh.Microbiological and clinical characteristics of diabetic foot infections in northern India. J Infect Dev Ctries 2012; 6(4):329-332.
2.Seema Nair, Sam Peter, Abhilash Sasidharan, Sujatha Sistla and Ayalur Kodakara Kochugovindan Unni. Incidence of mycotic infections in diabetic foot tissue. journal of culture collections 2006-2007;5:85-89
3.Sagar M. Miscellaneous fungi. In Gorbach S L, Bartlet JG, Blacklow N R, editors. Infectious diseases. Chapter 276, 3rd ed. 2004.p2270-5.
4.Nucci M, Anaissie E. Fusarium infections in immunocompromised patients. Clin Microbiol Rev. 2007; 20:695–704
5.Viswanathan Epidemiology of diabetic foot and management of foot problems in India. International Journal of Lower Extremity Wounds.2010;9:122-126.
6.Ekta Bansal, Ashish Garg, Sanjeev Bhatia , A K Attri, Jagdish Chander. Spectrum of Microbial Flora in Daibetic foot ulcers. IJPM 2008; 51(2):204-8.
7.Dipali A Chincholikar (Nee Kothari), Ramprasad B Pal. Study of Fungal and Bacterial infections of the diabetic foot. Indian J Pathol Microbiol 2002;45(1):15-22.
8.Chellan G, Shivaprakash S, Karimassery Ramaiyar S, Varma AK, Varma N, Thekkeparambil Sukumaran M, et al. Spectrum and prevalence of fungi infecting deep tissues of lower-limb wounds in patients with type 2 diabetes. J Clin Microbiol. 2010;48:2097–102.
9.Ramakrishna Pai, Rekha Boloor, Shreevidya K, Divakar Shenoy. Fusarium solani: An emerging fungus in chronic diabetic ulcer. Journal of laboratory physicians 2010;2(1):37-9.
10.Pinaki Dutta, A Premkumar, Arunaloke Chakrabarti, Viral N Shah, Arnanshu Behera, Depankar De et al. Fusarium falciforme Infection of Foot in a Patient with Type 2 Diabetes Mellitus: A Case Report and Review of the Literature. Mycopathologia 2013:DOI10.1007/s11046-013-9646-z
11.Mustafa Özyurt, Nurittin Ardıç, Kadir Turan, Şenol Yıldız, Oğuz Özyaral,Uğur Demirpek et al.The isolation of Fusarium sporotrichioides from a diabetic foot wound sample and identification. Marmara Medical Journal 2008;21(1);068-072
12.Saad J. Taj-Aldeen, Josepa Gene, Issam Al Bozom, Walter Buzina, Jose´ Francisco Cano & Josep Guarro. Gangrenous necrosis of the diabetic foot caused by Fusarium acutatum. Medical Mycology 2006; 44:547-52
13.Jagdish Chander.Textbook of Medical Mycology, New Delhi, Mehta Publishers, 2009;425-7.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: Nil
Copyright © 2013 Sahu Manisa,Bal Arun,Bhalekar Pallavi,Keny Dipti. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Article citation:-
Sahu Manisa,Bal Arun,Bhalekar Pallavi,Keny Dipti. Fusarium species: An emerging fungal pathogen in diabetic foot infections. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 December 37(37): 1957-1959. Available at www.jpbms.info.
Original article
Rajyalakshmi Gunti1,Usha Rani Anaparthy2,*,Durga Rani Arava1
Affiliation:-
1Assistant Professor,2Professor, Department of Microbiology,GGH campus, Rangaraya Medical College, Kakinada – 533008, Andhra Pradesh,India
Author’s contributions: - All the author contributed equally to this paper.
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, GGH campus, Rangaraya Medical College, Kakinada – 533008, Andhra Pradesh,India
*Corresponding author:-
Dr.Usha Rani Anaparthy, MD
Professor, Department of Microbiology, Rangaraya Medical College, Kakinada–533008,Andhra Pradesh, India
Abstract:
Aims and Objectives: The Present study was conducted in Government General Hospital, Kakinada from April - May 2013 to know the prevalence of Biofilm production in Staphylococcus aureus and coagulase negative Staphylococci and to compare the results of biofilm production by three different methods.
Material and Methods: A total Number of 50 Staphylococcus aureus and 50 coagulase negative Staphylococci isolated from different clinical samples were screened by tissue culture plate (TCP) method, tube method (TM) and Congo red agar (CRA) method for biofilm production.
Results: Among 50 Staphylococus aureus isolates screened, biofilm production was detected in 38(76%) by TCP method, 30(60%) by tube method and 42(84%) by CRA method, where as in 50 Coagulase negative Staphylococci it was 34(68%), 20(40%) and 40(80%) by three methods respectively.
Conclusion: In our study it was found that Congo red agar method is more sensitive when compared with other two methods for detection of biofilm production. It is also simple, easy to perform and economical.
Key words: Biofilm; Congo red agar; Staphylococcus; Tissue culture plate; Tube method.
REFERENCES
1.Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet 2001; 358: 135-8.
2.Carsten Matz, Jeremy S. Webb, Peter J. Schupp, Shui Phang, Anahit Penesyan, Suhelen Egan, Peter D. Steinberg, Staffan Kjelleberg: Marine biofilm bacteria evade eukaryotic predation by targeted chemical defense. PLoS ONE published July 23, 2008, doi/ pone.0002744.
3.“ Research on Microbial Biofilms (PA-03-047)”. NIH, National Heart, Lung and Blood institute. 2012-12-20.
4.Rogers A H (2008). Molecular oral Microbiology. Caister academic press. pp 65-108. ISBN 97A-1-904455-24-0.
5.Imamura Y, Chandra J, Mukharjee PK, et al. Fusarium and Candida albicans biofilms on soft contact lenses: model development, influence of lens type and susceptibility to lens care solutions. Antimicrobial agents and Chemotherapy 2008 Jan;52 (1): 171 – 82. doi: 10.1128/AAC.00387-07. PMC 2223913. PMID 17999966.
6.Lewis K. Riddle of Biofilm resistance. Antimicrobial agents and chemotherapy 2001 April; 45 (4): 999-1007. Doi; 10.1128/AAC.45.4.999-1007.2001. PMC90417. PMID11257008.
7.Parsek MR, singh PK. Bacterial biofilms : an emerging link to disease pathogenesis. Annual review of Microbiology 2003;57: 677–701. Doi:10.1146/annurev. micro.57.030502.090720.PMID 14527295.
8.Chaudhary A, M Nagaraja and A.G Kumar. Potential of Biofilm formation by staphylococci on polymer surface and its correlation with methicillin susceptibility. Ind. J. Med. Microbiol 2009;27: 377-378.
9.Gerke C, Kraft A, Sussmuth R, Schweitzer O, Gotz F. Characterisation of N- acetylglucosaminyl transferase activity involved in the biosynthesis of the Staphylococcus epidermidis Polysaccharide intercellular adhesion. J Biol Chem 1998: 273: 18586 – 93.
10.Cramton SE, Gerke C, Schell NF, Nichol WW, Gotz F. The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infect Immun 1999;67:5427-33.
11.Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun 1982; 37: 318-26.
12.Christensen GD, Simpson WA, Younger JA, Baddour LH, Barrett FF, Melton DM et al. Adherence of coagulase negative Staphylococci to Medical devices.
J clin Microbiol 1985; 22: 996- 1006.
13.Freeman DJ, Falkiner FR, Keane CT. New method for detecting slime production by coagulase negative Staphylococci. J Clin Pathol 1989;42: 872-4.
14.Ludwicka A, switalski LM, Lundin A, pulverer G, wadstrom T. Bioluminescent assays for measurement of bacterial attachment to polyethylene. J Microbiol methods 1985; 4: 169-77.
15.Zufferey J, Rime B, Francioli P, Bille J. Simple method for rapid diagnosis of catheter associated infection by direct Acridine orange staining of catheter tips. J Clin Microbiol 1998; 26:175-7.
16.T Mathur, S Singhal , S kahn, DJ upadhyay, T Fatima, A Rattan. Detection of Biofilm formation among the clinical isolates of Staphylococci. An evaluation of three different methods. Ind Journal of Med Microbiol 2006;24(1): 25-9.
17.Bose S, M. Khodke, S. Basak and S. K. Mallik, 2009. Detection of Biofilm producing staphylococci: Need of the hour. J. Clin. Diagnostic Res 3: 1915-1920.
18.Fathima Khan, Indu Shukla, Meher Rizvi, Tariq Mansoor and S.C. Sharma, 2011. Detection of Biofilm formation in Staphylococcus aureus. Does it have a role in Treatment of MRSA infections. Trends in Medical research, ISSN 1819-3587 / DOI: 10.3923/tmr.2011.
19.R Srinivasa Rao, R Uma Karthika, SP singh, P Shashikala, R Kanungo, S Jaya chandra, K Prasanth. Correlation between Biofilm production and multiple drug resistance in Imipenem resistant clinical isolates of Acinetobacter Baumanii. Ind Journal of Med Microbiol 2008; 26(4):333-7.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Source of support: Nil
Copyright © 2013 Rajyalakshmi Gunti,Usha Rani Anaparthy,Durga Rani Arava. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Article citation:-
Rajyalakshmi Gunti,Usha Rani Anaparthy,Durga Rani Arava. Detection of biofilm production in Staphylococcus aureus and coagulase negative Staphylococci using three different methods. Journal of pharmaceutical and biomedical sciences (J Pharm Biomed Sci.) 2013 December 37(37): 1952-1956. Available at www.jpbms.info.