DocumentsDate added
Original Article
Iyanda Ayobola A*
Affiliation:-
Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria
*To whom it corresponds:-
Iyanda Ayobola A.
Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria
Phone No +2347039407465+2347039407465
Abstract
Background/aim: Exposure to kerosene is common in the developing world where it is used as fuel for cooking purposes. The objective of this study was to investigate the activities of the endogenous antioxidant enzymes; catalase, superoxide dismutase (SOD), glutathione peroxidase, glutathione-S-transferase and glutathione reductase as well as levels of malondialdehyde, reduced and oxidized glutathione in rats that had been exposed to kerosene through either the oral or dermal route and to compare 1 week response with that of 3 weeks. Materials & Methods: Thirty rats were randomly divided into five groups. Groups 1 & 2 were administered with 0.3 ml/kg body weight of kerosene through oral and dermal routes respectively and the study terminated after 1 week. Groups 3 (oral) and four (dermal) received the same treatment as groups 1 and 2 but the study was terminated after 3 weeks of exposure. Group five served as the control. Blood samples were collected after each administration period.
Results: Irrespective of route or duration of exposure, activities of antioxidant enzymes were significantly reduced (p<0.05) except SOD that was not significantly different (p>0.05) in dermal group after 1 week of kerosene administration. Moreover, reduced glutathione and reduced/oxidized glutathione ratio were significantly decreased (p<0.05) while MDA and oxidized glutathione were significantly increased (p<0.05).
Conclusion: Exposure to trace quantity of kerosene is toxic to the antioxidant defense system of Wistar rats even as early as the first week of exposure.
Keywords: Female Wistar rats; Kerosene; Oxidative stress.
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Article citation:
Iyanda A.A. Two different durations of exposure: A comparison of the effects of trace quantity of kerosene on serum antioxidant indices in wistar rats. J Pharm Biomed Sci 2014; 04(03): 215-221. Available at www.jpbms.info.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Iyanda A.A. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Research article:
Lakshminarasimhaiah1,*, S. Alexander2
Affiliation:-
1Prasad Institute of Pharmaceutical Sciences, Jangaon-506167, Warangal District, Andhra Pradesh, India.
2J.S.S College of Pharmacy, Rocklands, Post Box No:20, Ootacamund-643 001, Tamil Nadu, India
The name of the department(s) and institution(s) to which the work should be attributed:
Prasad Institute of Pharmaceutical Sciences, Jangaon-506167, Warangal District, Andhra Pradesh, India
J.S.S College of Pharmacy, Rocklands, Post Box No: 20, Ootacamund-643 001, Tamil Nadu, India
*To whom it corresponds:-
Lakshminarasimhaiah.
Prasad Institute of Pharmaceutical Sciences, Jangaon-506167, Warangal District, Andhra Pradesh, India
Abstract
In the present investigation a series of pyrazole derivatives were synthesized by condensation of 8-acetyl, 7-hydroxy, 4-methyl coumarin(I) with substituted benzaldehyde to form chalkone (II). The obtained chalkone was treated with hydrazine to form pyrazole derivatives(IIIa-h). The synthesized compounds were characterized by IR, NMR, Mass spectra and elemental analysis. The synthesized compounds were evaluated for analgesic activity. The synthesized compounds showed good analgesic activity compared to standard drug.
Keywords: Analgesic activity; ESIMS; 1H NMR; IR; Pyrazole.
Article citation:
Lakshminarasimhaiah, S. Alexander. Synthesis and Biological Evaluation of New Pyrazole Derivatives. J Pharm Biomed Sci 2014; 04(03): 267-272. Available at www.jpbms.info.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Lakshminarasimhaiah, S. Alexander. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Manjula Shantaram1,2,*, Anusha M.S.3, Chethana3
Affiliation:-
1Professor of Biochemistry, Mangalore University, PG Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India
2Professor of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India
3Post Graduate Students, Department of Biochemistry, St. Aloysius College, Mangalore, Karnataka, India
The name of the department(s) and institution(s) to which the work should be attributed:
1. Mangalore University, PG Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India
2. Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India
3. Department of Biochemistry, St. Aloysius College, Mangalore, Karnataka, India
*To whom it corresponds:-
Dr. Manjula Shantaram.
Professor of Biochemistry, Mangalore University, PG Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India
Abstract
Diabetes mellitus is a major worldwide health problem leading to markedly increased mortality and serious morbidity. Literature demonstrates increased adenosine deaminase activity in type 2 diabetes mellitus than in non- diabetics. Adenosine deaminase (ADA) is an enzyme that has been suggested to be important for modulating the bioactivity of insulin. It is a metalloenzyme which catalyzes the irreversible deamination of adenosine and deoxyadenosine to inosine and deoxyinosine and has an important role in regulating adenosine concentration. This study aims to correlate the association of adenosine deaminase with type 2 diabetes mellitus patients. The subjects included in this study consisted of 20 normal healthy individuals who served as controls with no history of DM and 20 patients with type 2 diabetes mellitus both males and females in the age group of 25-50 years on oral hypoglycemic drugs. In our study, the activity of serum ADA was measured according to the method of Giusti. ADA was estimated in all the subjects under study. ADA level was found to be decreased in the patients of type 2 DM as compared to controls. Moreover, the patients were the confirmed diabetes cases and were undergoing treatment for their disease. It is possible that the decrease in ADA levels may be due to the depressed cell mediated immunity in the patients due to the intake of medicines to control their blood sugar. Results suggest that reduced serum ADA activity reflects decrease in ADA 2 activity.
Keywords: Diabetes Mellitus (DM); Adenosine deaminase(ADA); Type 2 Diabetes Mellitus; Serum.
Article citation:
Manjula Shantaram, Anusha M.S, Chethana. Serum Adenosine Deaminase activity in Type 2 Diabetes Mellitus. J Pharm Biomed Sci 2014; 04(03): 246-248. Available at www.jpbms.info.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Manjula Shantaram, Anusha M.S, Chethana. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article:
Yogeesha babu K.V1,*,Manjula R.2,Kruthika P3,Viswajeet Bardoloi4
Affiliation:-
1Professor, Department of Microbiology, Faculty of Medicine, Jazan University, Jazan, Saudi Arabia
1Former Professor, Department of Microbiology. Infection Control officer, Hospital Infection Control Committee. SS Institute of Medical Sciences and Research Centre, Jnanashankara, NH-4 Bypass road, Davangere, India
2Under graduate student, MBBS PHASE III, SS Institute of Medical Sciences and Research Centre, Jnanashankara, NH-4 Bypass road, Advancer, India
3,4Post graduate students, Department of Microbiology, SS Institute of Medical Sciences and Research Centre, Jnanashankara, NH-4 Bypass road, Davangere, India
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Microbiology, Faculty of Medicine, Jazan University, Jazan,Saudi Arabia
2. SS Institute of Medical Sciences and Research Centre, Jnanashankara, NH-4 Bypass road, Davangere, India
*To whom it corresponds:-
Dr.Yogeesha babu K.V.
Professor, Department of Microbiology,Faculty of Medicine, Jazan University, Jazan , Saudi Arabia
Email: dr.yogeshb77@yahoo.com
Phone: Mobile: 00965575728050096557572805
Abstract
Background: Pseudomonas aeruginosa is a common pathogen in intensive care units [ICUs] attended with higher mortality and morbidity. Of the multiple environmental sources sinks were hypothesized as the important source since hands of health care workers carrying multiple nosocomial pathogens will be washed in sinks.
Objectives: To determine rate of sink colonization/contamination and role of P.aeruginosa from sinks as source and surrogate marker of circulating P. aeruginosa antibiogram types among patients in ICUs.
Materials and Methods: Cross sectional study of [13 sinks from 7 ICUs] sinks was conducted with three specimens per sink; Interior of the tap, Knobs/Handles and basins, collected twice at an interval of four weeks and processed as per standard laboratory procedures. Antibiogram types of P.aeruginosa from sinks were correlated with nosocomial infections in respective ICU by identical antibiogram type from sink and patients along with temporospatial association.
Results: Rate of ICU sink colonization was 46.15 %(6/13) and 66.67%(8/12) with nine(PA-1 to PA-9) and eight (PA-10 to PA-17) antibiogram types of P.aeruginosa respectively in two sets of sink specimens. Sink colonization was highest at basin [38.47%(4/13), 66.67%(8/12) ]and lowest at knob/handles and interior of the tap [7.69%(1/13),16.67%(2/12)] respectively. Thirteen antibiogram types of P.aeruginosa were correlated with 17 nosocomial infections. None of the general ward sinks and sinks in NSICU and NICU yielded P.aeruginosa. Six Imipenem resistant P.aeruginosa antibiogram types were isolated from sinks.
Conclusions: ICU sinks act as source/reservoir of P.aeruginosa in nosocomial infections. Antibiogram types of Pseudomonas aeruginosa in ICU sinks do not act as surrogate marker for circulating antibiogram types of Pseudomonas aeruginosain ICUs.
Keywords: Pseudomonas aeruginosa; sinks; Source/reservoir; Surrogate marker of nosocomial pathogens.
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Article citation:
Yogeesha babu K.V,Manjula R,Kruthika P,Viswajeet Bardoloi. Role of Sinks in ICUs as Reservoirs and/or Surrogate Markers of Pseudomonas Aeruginosa in a Tertiary Care hospital. J Pharm Biomed Sci 2014; 04(03): 169-175. Available at www.jpbms.info.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Yogeesha babu K.V,Manjula R,Kruthika P,Viswajeet Bardoloi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research Article
Suman Gupta1, Nadeem Khan2,*, Ragini Gothalwal2
Affiliation:-
1Contractual lecturer, Department of Biochemistry and Genetics, Barkatullah University, Bhopal, M.P, India- 462026.
2Department of biotechnology and bioinformatics centre, Barkatullah University, Bhopal, M.P, India- 462026.
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Biochemistry and Genetics, Barkatullah University, Bhopal, M.P, India- 462026
Department of Biotechnology and Bioinformatics centre, Barkatullah University, Bhopal, M.P, India- 462026.
NEERI, NAGPUR (M.H.), India
*To whom it corresponds:-
Nadeem Khan,
Research Scholar, Department of Biotechnology and Bioinformatics centre, Barkatullah University, Bhopal, M.P,India- 462026.
Abstract:
Increased oxidative stress is a widely accepted participant in the development and progression of diabetes and its complications. Due to extra generation of free radicals in diabetic condition, the antioxidant status also gets disturbed needing proper attention and care. Evaluation of antioxidant activity of the herbal formulation could be done by assaying the activities of the enzymes Glutathione reductase, Glutathione peroxidase, superoxide dismutase and determination of the superoxide accumulation. Study was design into four groups, Group I(NH), were normal healthy subjects, Group II(DI), were diabetics, group III (TTD)were tolbutamide treated diabetic patients, group IV(HFTD), were diabetic patients receiving combination herbal formulation in the, dosage of 5 gm/day for a period of 4 weeks. Results were exciting with herbal formulation since it was found to effective in correction of cell parameters related to phagocytosis and remarkable recovery in anti-oxidant enzymes in diabetic patients, which serve as good adjuvant in the present armamentarium of anti-diabetic drugs for management of later diabetic complication.
Keywords: DM (diabetic mellitus); ROS (Reactive oxygen species); Phagocytosis, SOD; PMNL.
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Article citation:
Suman Gupta, Nadeem Khan, Ragini Gothalwal. Role of poly-herbal drug formulation on polymorphoneuclear leucocyte anti-oxidant enzymes in quenching of oxidative stress from diabetic individuals. J Pharm Biomed Sci 2014; 04(03): 183-188. Available at www.jpbms.info.
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Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Suman Gupta, Nadeem Khan, Ragini Gothalwal. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited