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Research article
Nuha M.E. Agabna1 , Sania A.I. Shaddad2,*, A.K. Mudathir3
Affiliation:-
1 Department of Pharmacology, Faculty of Pharmacy University of Khartoum, Sudan
2 Deptartment of Medicine (Pharmacology), Faculty of Medicine University of Khartoum, Sudan
3 Department of Pharmacogonosy, Faculty of Pharmacy University of Khartoum, Sudan
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Pharmacology, Faculty of Pharmacy University of Khartoum, Sudan
*To whom it corresponds:-
Dr Nuha ME Agabna
Department of Pharmacology), Faculty of Pharmacy University of Khartoum, Sudan
Contact no:-++249912564229
Abstract
Lawsonia inermis, family Lythraceae (henna) is used traditionally to manage many conditions. This study evaluated the effects of 500 and 1000g/Kg dose of ethanolic seeds extracts on haematologic parameters, serum electrolytes, urea, creatinine, liver enzymes, plasma proteins, lipids profile, and random blood glucose.
Method: A single oral dose of 500mg and 1000mg /Kg was administered to two groups of mice to study acute toxicity. The extract was administered orally daily for 30 days to two groups of five animals each; one receiving 500mg/Kg and the other 1000mg/kg, a third group was control given normal saline 10ml/Kg. Blood samples were taken at days 0, 15 and 30 and analyzed for complete haemogram, kidney and liver function. By the end of the experiment animals were sacrificed, examined post mortally, histological slides of vital organs prepared to study the effects.
Results: The acute dose of 500mg and 1000mg /Kg caused no death in animals after 24 hours, and no signs of change in feeding, behavior, diarrhea or loss of fur were observed.
WBC, RBC haemoglobin and platelets count were minimally affected. Serum Na and K were not deviated from control, and so for creatinine and urea. Liver enzymes were not affected by chronic administration of henna extract. However a slight but significant elevation in AST was recorded in the high dose group. Plasma proteins were not affected. The lipids profile was similar to control group. Random blood glucose was same as the control group (within the limits). Post mortem examination showed no signs of toxicity. Observing feeding and animals behavior no differences were seen.
Conclusion: The ethanolic extract of L. inermis seeds at the studied doses is safe with no detrimental effects.
Keywords: Lawsonia inermis, safety, haematology, renal function, liver function.
REFERENCES
1.Muhammad HS, Muhammad S. The use of Lawsonia inermis linn. (henna) in the management of burn wound infections. African Journal of Biotechnology 2005; 4 (9): 934-937
2.Takeda Y et al. New phenolic glycosides from Lawsonia. 198851(4):725-729
3.Sudharameshwri and Radhika, 2007: Sudharameshwari K, Radhika J. Antibacterial screening of aegle marmelos, lawsonia inermis and albizzia libbeck. African Journal of Tradional and Complementary Medicine. 2007;4(2):199–204
4.Sarwar M, Niwa M, Sakai T, Gupta S. 24[beta]ethyoxycholest4—en-3beta-ol from root of L. inermis. Physochemisty 1992;31(7)2558-2560.
5.Uma DB, CW Ho, WM Wan Aida. Optimization of extraction parameters of total phenolic compounds from henna (Lawsonia inermis) leaves. Sains Malaysiana, 2010 39(1):119-128.
6.Cuong NX, Nguyen NX, Thao NP, Nam NH, Dat NT, Anh HLT, Huong LM, Kiem PV, Minh CV, Won JH, Chung WY, Kim YH. Inhibitors of osteoclastogenesis from Lawsonia inermis leaves. Bioorganic and Medicinal Chemistry Letters 2010; 20(6):4782-4784.
7.Wong KC, Teng YE. Volatile components of Lawsonia inermis L. flowers. Journal of essential oil research 1995;7(4):425-428.
8.Nayak BS, Isitor G, Davis EM, Pillai GK. The evidence based wound healing activity of Lawsonia inermis Linn. Phytotherapy Research. 2007;21(9):827-31.
9.Khiljee S, Rehman SN, Khiljee T, RS, Khan MY, Qureshi UA. Use of traditional herbal medicines in the treatment of eczema. Journal of Pakistan Association of Dermatologists. 2011;21:112-117.
10.Khan, MS, Aleem MMH, Shagufta. Effect of Psoralia corylifolia Linn. and Marham-e-Gulabi in Da-al-sadaf (psoriasis) 2009.Indian Journal of Traditional Knowledge. 2009;8(3):425-430.
11.Yucel I, Guzin G. Topical henna for capecitabin induced hand-foot syndrome. Invest new drugs 2008; 26:188-192.
12.Hashmi S, Begum W, Sultana A. Efficacy of Sphaeranthus indicus and cream of Lawsonia inermis in cervical erosion with cervicitis. European Journal of Integrative Medicine 2011; 3(3): e183-e188.
13.Alia BH, Bashi AK, Tanira MOM Anti-Inflammatory, antipyretic, and analgesic effects of Lawsonia inermis L.(Henna) in rats. Pharmacology 1995;51:356-363.
14.Gupta SS. Prospects and perpectives of natural plants products in medicine. Indian Journal of Pharmacology. 1994; 26:1-12.
15.Mikhaeil BR, Badria FA, Maatooq GT, Mhamed MA Antioxidant and Immunomodulatory Constituents of Henna Leaves- Zeitschrift fur Naturforsch., 2004 59c:468-476.
16.Chaudhary GD, Poonia P, Kamboj P, Kalia AN. Hepato-protective potential of Lawsonia inermis L. seeds. International Journal of Phytopharmacology 2012; 3(1):012:66-73.
17.Latha PG, Suja SR, Hyamal S, Ajasekharan SR. Some hepatoprotective garden plants. Natural Product Radiance 2005; 4:278-279.
18.Dasgupta T, Rao AR, Yadava PK. Modulatory effect of Henna leaf (Lawsonia inermis) on drug metabolising phase I and phase II enzymes, antioxidant enzymes, lipid peroxidation and chemically induced skin and forestomach papillomagenesis in mice. Molecular and cellular biochemistry.2003; l245(1-2):11-22.
19.Hsounaa AB, Triguia M, Culiolib G, Blacheb Y, Jaouaa S. Antioxidant constituents from Lawsonia inermis leaves: Isolation, structure elucidation and antioxidative capacity. Food Chemistry.2011;125(1): 193-200.
20.Guha G, Rajkumar V, Kumar RA, Mathew L. Antioxidant activity of Lawsonia inermis extracts inhibits chromium iv –induced cellular and DNA toxicity. Natural Products Research; 2009; 23(18):1740-5.
21.Endrini SE, A Rahamat, Isail P, Taufiq-Yap YH, Anticarcinogenic properties and antioxidant activity of Henna (Lawsonia inermis L.) Journal of Medical Sciences 2002; 2 (4) 194-197.
22.Makhija IK, Dhananjaya DR, Kumar V, Devkar R, Khamar D, Manglani N, Chandrakar S Lawsonia inermis–from traditional use to scientific assessment. African journal of pharmaceutical sciences and pharmacy. 2011;2(1):145-165.
23.Abdel-Wahab SI , Ain NM , Abdul AB , Elhassan MM , Tengku TA Ibrahim . Energy dispersive X-ray microanalysisi of elements content of medical plants used traditionally as anticancer cure. Research Journal of biological Sciences 2009; 4(5): 547-549.
24.Endrini SE, A rahamat, Isail P, Taufiq-Yap YH, 2007 Comparing the toxicity properties and mechanism of Lawsonia inermis and Sirobilanther crispus extract against several cancer cell lines. Journal of Medical Sciences 2007; 7(7):1098-1102.
25.Borade AS, Kale BN, Shete RV. A phytopharmacological review on Lawsonia inermis (Linn.) International Journal of Pharmcology and Life Sciences. 2011;2(1):536-541.
26.Ozaslan M, Zumrutdal ME, Daglioglu K, Kilic IH, et al Isik Didem Karagoz ID, M.E. Kalender, Muhittin Tuzcu, Omer Colak and Beyhan Cengiz. Antitumoral effect of L. inermis in mice with EAC. International Journal of Pharmacology. 2009;5(4):263-267.
27.Zumrutdal ME, Ozaslan M, Tuzcu M, Kalender ME, Daglioglu K, Akova A, ID Karagöz, IH Kilic IH, O Colak O, F Köksal F. Effect of Lawsonia inermis treatment on mice with sarcoma. African Journal of Biotechnology 2008;7(16):2781-2786.
28.Syamsidin, Inwati, Winarno H. The effect of Inai (lawsonia inermis linn) leaves extract on blood sugar levels: an experimental study Research Journal of Pharmacology 2008;2(2):20-23.
29.Sultana N; Choudhary MI, Khan A. Protein glycation inhibitory activities of Lawsonia inermis and its active principles. Journal of Enzyme Inhibition and Medicinal Chemistry.2009; 24(1):257-261.
30.Arayne MS, Sultana N, Mirza AZ. In vitro hypoglycemic activity of methanolic extract of some indigenous plants. Pakistan Journal of Pharmceutical Science 2007; 20(4): 261-268.
31.Akter A, Neela FA, Khan MSI, Islam MS, Alam MF. Screening of ethanol, petroleum ether and chloroform extracts of medicinal plants, Lawsonia inermis L. and mimosa pudica L. for antibacterial activity. Indian Journal of Pharmaceutical Sciences.2010;72(3):388–392.
32.Babu PD, Subhasree RS. Antimicrobial Activities of Lawsonia inermis - A review. Academic Journal of Plant Sciences 2009;2(4):231-232.
33.Saadabi MA . Evaluation of Lawsonia inermis Linn (Sudanese Henna) leaf extracts as antimicrobial agent. Research journal of biological sciences 2007; 2(4): 419-423.
34.Sauriasari R, Wang DH, Takemura Y, Tsutsui K, Masuoka N, Sano K, Horita M, Wang BL, Ogino K. Cytotoxicity of lawsone and cytoprotective activity of antioxidants in catalase mutant Escherichia coli. Toxicology. 2007;235(1-2):103-11.
35.Aqil F, Khan MS, Owais M, Ahmad I. Effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant Staphylococcus aureus. Journal of Basic Microbiology 2005;45(2):106-14.
36.Habbal OA, Al Jabri AA, El Hag AH, Al Mahroogi ZH, AL Hashimi NA. In vitro antimicrobial activity of Lawsonia inermis Linn a pilot study on Ommani henna. Suadi Medical journal. 2005;26(1):69-72.
37.Sharma A , Patel VK, Ramteke P. Identification of vibriocidal compounds from medicinal plants using chromatographic fingerprinting. World Journal of Microbiology and biotechnology.2009; 25(1):19-25 .
38.Sharma A, Patel VK, Ramteke P. Identification of vibriocidal compounds from medicinal plants using chromatographic finger printing World Journal of Microbiology and Biotecnology.2008; 25(1) 19-25.
39.Berenji F, Rakhandeh H, Ebrahimipour HE. Invitro study of the effects of henna extracts Llawsonia inermis linn) on Malassezia species. Jundishapur Journal of Microbiology. 2010;3(3):125-128.
40.Okpekon T, Yolou S, Gleye C, Roblot F, Loiseau P, Bories C, Grellier P, Frappier F, Laurens A, Hocquemiller R. Antiparasitic activities of medicinal plants used in Ivory Coast. Journal of Ethnopharmacology. 2004; 90(1):91-7.
41.Atawodi SE, Ameh DA, Ibrahim S, Andrew JN, Nzelibe HC, Onyike EO, Anigo KM, Abu EA, James DB, Njoku GC, Sallau AB. Indigenous knowledge system for treatment of trypanosomiasis in Kaduna state. Nigerian Journal Ethnopharmacology. 2002;79(2):279-82.
42.Aguwa CN. Toxic Effects of the Methanolic Extract of Lawsonia inermis Roots. Pharmaceutical Biology 1987;25(4):241–245.
43.Munshi SR, Shetye TA, Nair RK . Antifertility activity of three indigenous plant preparations Planta Medica 1977; 31: 73-75.
44.Maurya, Gupta Traditional herbs for medicine: pioneering efforts by Indian Central drug research Instittute. Technology Monitor 2006;11(11):1431-1450.
45.Agabna N, Shaddad S, Tybe I. Antifertility effects of L. inermis and A. precatorius on female albino rats. PhD thesis, Faculty of Pharmacy University of Khartoum. 2013.
46 OECD 1992a. Acute oral toxicity. In the testing guidelines for chemicals. OECD publication No 401. OECD Paris.(OECDa, 1992)46.
Article citation:
Nuha Agabna ME, Sania Shaddad AI, Mudathir AK. Safety of Lawsonia inermis ethanolic seeds extract. J Pharm Biomed Sci. 2014; 04(43):303-309. Available at www.jpbms.info.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Nuha Agabna ME, Sania Shaddad AI, Mudathir AK. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
R. Sangeetha Kumari1,*, V.Kaviyarasan2
Affiliation:-
1Research Scholar, 2Assistant Professor, CAS in Botany, University of Madras, Guindy campus, Chennai –600025,India
2Assistant Professor,CAS in Botany, University of Madras, Guindy campus, Chennai – 600 025.
The name of the department(s) and institution(s) to which the work should be attributed:
CAS in Botany, University of Madras, Guindy campus, Chennai –600025, India
*To whom it corresponds:-
R. Sangeetha Kumari.
Research Scholar, CAS in Botany, University of Madras, Guindy campus, Chennai –600025, India
Email: sangkumari@gmail.com, Ph: +919092875300
Abstract
The siderophores produced by fungal organisms were screened from the both culture supernatants and solid medium by the universal chrome assural assay (CAS assay). All the screened organisms are comes under the hydroxymate type of siderophores using Iron Percholate assay at 480nm. The effect of iron concentration and complexity of media has been studied for siderophore production in the edible and medicinal mushrooms Agaricus sp and Lentinus sp. The siderophore coding genes were studied using random amplification with P1 and P2 primers.
Keywords: Chrome Azurol Sulphonate (CAS); deferroxamine; hydroxymate; carboxylate; catecholates; basidiomycetes.
REFERENCES
1.D. B. Alexander, D. A. Zuberer. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biology and Fertility of Soils. 1991;12: 39-45.
2.Arnow LE. Colorimetric determination of the components of 3,4-dihydroxyphenylalanine-tyrosine mixtures. J. Biol.Chem. 1937; 118:531-537.
3.Atkin CL, Neiland JB, Phaff HJ. Rhodotorulic acid from species of Leucosporidium, Rhodospondium, Rhodotorula, Sporidiobolus and Sporobolomyces, and a new alanine- containing ferrichrome from Cryptococcus meliviosu.ni. J Bacteriol. 1970; 103:722-73
4.A.L. Crumbliss,. Aqueous Solution Equilibrium and Kinetic Studies of Iron Siderophore and Model Siderophore Complexes, Handbook of Microbial Iron Chelates, G. Winkelmann, Ed., CRC Press, 1991, Chap. 7. (invited).
5.Baakza A, Vala AK, Dave BP, et al. A comparative study of siderophore production by fungi from marine and terrestrial habitats. J. Exp. Mar. Biol. Ecol. 2004; 311:1–9.
6.S. Dhungana & A.L. Crumbliss. Coordination chemistry and redox processes in siderophore-mediated iron transport. Geomicrobiology. Journal, 2005;22:87-98.
7.Fekete, F.V. Chandhoke and J. Jellison. Iron binding compounds produced by wood decaying basidiomycetes. Appl.Environ.Microbiol.1989; 55:2720-2722.
8.Frey-Klett, P., Chavatte, M, Clausse, M.L., Courrier, S, Le Roux, C, Raaijmakers, J, Martinotti, M.G., Pierrat, J.C. and Garbaye, J. Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads. New phytologist. 2005; 165: 317-328.
9.Hu, C. J., C. Bai, X. D. Zheng, Y. M. Wang, and Y. Wang. Characterization and functional analysis of the siderophore-iron transporter CaArn1p in Candida albicans. J. Biol. Chem. 2002;277:30598–30605.
10.Ismail A, Bedell GW, Lupan DM. Siderophore production by the pathogenic yeast Candida albicans. Biochem Biophys Res Comm.1985; 130:885–891.
11.Jelliso, J., B. Goodell, F.Fekete and V. Chandhoke, 1990. Fungal siderophores and their role in wood degradation. International Research group on wood preservation DOC.NO.IRG/WP/1442. IRG Secretariat, Stockholm, Sweden. 16PP.
12.Martin JD, Ito Y, Homann VV, et al. Structure and membrane affinity of new amphiphilic siderophores produced by Ochrobactrum sp. SP18. J Biol Inorg Chem. 2006;11:633–641. [PubMed]
13.Oberegger, H., M. Schoeser, I. Zadra, B. Abt, and H. Haas. SREA is involved in regulation of siderophore biosynthesis, utilization and uptake in Aspergillus nidulans. Mol. Microbiol. 2001; 41:1077–1089.
14.Reigh Geraldine, 1991. Analysis of siderophore production by Rhizobium meliloti 220-5. PhD thesis, Dublin City University.
15.Renshaw JC, Robson GD, Trinci APJ, et al. Fungal siderophores: structures, functions and applications. Mycol. Res. 2002;106:1123–1142.
16.Santose, R., N. Buisson, S. Knight, A. Dancis, J.M. Camadro, and E. Lesuisse. Haemin uptake and use as an iron source by Candida albicans: role of CaHMXI encoded haem oxygenase. Microbiology. 2003;149: 579-588.
17.Schroder, I., E. Johnson, and S.de Vries. Microbial ferric iron reductases. FEMS Microbiol. Rev.2003; 27:427–447.
18.Schwyn, B., and Neilands, J.B. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 1987; 160:47-56.
19.Steglich W, Steffan B, Stroech K, Wolf M. Pistillarin. A characteristic metabolite of Clavariadelphus pistillaris and several Ramaria species (Basidiomycetes) Z Naturforschung, C. 1984; 39C:10–12.
20.Winkelmann G. Microbial siderophore-mediated transport. Biochem Soc Trans. 2002; 30(4):691-6.
Article citation:
Sangeetha RK, Kaviyarasan V. Screening of Siderophores in Basidiomycetes. J Pharm Biomed Sci 2014; 04(04): 291-297. Available at www.jpbms.info.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Original article:
Trivedi Grishma1,*, Nerurkar Alka B2
Affiliation:-
1Assistant Professor, 2Professor & Head, Microbiology Department, GMERS Medical College & Hospital, Valsad, Gujarat, India.
The name of the department(s) and institution(s) to which the work should be attributed:
Microbiology Department, GMERS Medical College& Hospital, Valsad, Gujarat, India
*To whom it corresponds:-
Dr. Grishma Trivedi.
Assistant Professor, Department of Microbiology, GMERS Medical College & Hospital, Valsad, Gujarat, India.
Email: drgrtrivedi@gmail.com
Abstract:
Introduction: HIV infection is a global epidemic. In India, HIV was first detected in commercial sex workers in Tamil Nadu in 1986 and since then, the infection is growing quite fast with geographic variation. The epidemiology of HIV in rural India is not well known and rural residents have been underrepresented, despite 67% of HIV infected people live in rural areas.
Aim: To find out the sero-prevalence of HIV infection in a rural tertiary care teaching hospital.
Material & Method: A retrospective analysis was carried out, of 3802 participants enrolled at Integrated Counseling and Testing Center (ICTC) attached to our tertiary care teaching hospital. HIV sero-positive participants were further analysed on the basis of age, sex and possible routes of transmission.
Results: Out of 3802, 1909 (50.21%) were males, 1887 (49.63%) were females and 06 (0.16%) were Trans-sexual/Transgender (TS/TG). Amongst the total of 3802 participants, 175 (4.6%) were proved to be positive for HIV-1 infection. Prevalence of HIV infection among males was 104 (5.45%), females was 67 (3.55%) and TS/TG was 4 (66.7%); while the most common transmission route was heterosexual (n=158, 90.3%), followed by vertical (n=15, 8.6%) and homosexual/Bisexual (n=2, 1.14 %).
Conclusion: More studies are required for population residing in rural areas as HIV epidemic appears to be moving from urban to rural populations..
Keywords: HIV; Rural tertiary care hospital; Sero-prevalence; Trans-sexual/Transgender population.
REFERENCES
1.Fauci AS, Lane HC. Human immunodeficiency virus disease: AIDS and related disorders. In: Kasper DL, Fauci AS, Longo DL, Braunwald E, Hauser SL, Jameson JL, eds. Harrison's Principles of Internal Medicine, 18th ed. USA: The McGraw-Hill companies, Inc., 2012; pg:1506-1587.
2.AIDS epidemic, 2006: AIDS epidemic update 2007. (Online) Cited 2009 April). Available from URL:http://data.unaids.org/pub/EPISlides/2007/2007_epiupdate_en.pdfg.
3.Godbole S, Mehendale S. HIV/AIDS epidemic in India: risk factors, risk behavior & Strategies for prevention & control. Ind J Med Res 121, April 2005, pp 356-368.
4.National AIDS Control Organization. Ministry of Health & Family Welfare, Government of India. HIV Sentinel Surveillance 2006 India Country Report. Available from: http://www.nacoonline.org/Quick_Links/Publication/ME_and_Research_Surveillance/Reports_and_Surveys/HIV_Sentinel_Surveillance_2006_India_Country_Report/
5.NACO (2007) Guidelines on HIV Testing. National AIDS Control Organisation Available: http://www.nacoonline.org/upload/Final%20Publications/Blood%20Safety/GUILDELINES%20FOR%20HIV%20TESTING.pdf. Accessed 12 December 2013.
6.Mir MA et al. Clinical and demographic profile of HIV/AIDS patients diagnosed at a tertiary care center in Kashmir. J Pak Med Assoc June 2010; Vol. 60, pg:428-431.
7.Gerardo Alvarez-Uria et al. Gender differences, routes of transmission, socio-demopgraphic characteristics and prevalence of HIV related infections of adults and children in an HIV cohort from a rural district of India. Infectious Disease Reports 2012; Volume 4:e19, pg 66-70.
8.Hall HI, Song R, Rhodes P, Prejean J, An Q, Lee LM, et al. Estimation of HIV incidence in the United States. JAMA 2008; 300:520-9.
9.May M, Gomples M, Delpech V, et al. Impact of late diagnosis and treatment on life expectancy in people with HIV-1: UK Collaborative HIV Cohort (UK CHIC) Study. BMJ 2011;343.
10.Vyas KJ et al. A comparison in HIV-associated stigma among healthcare workers in urban and rural Gujarat. Journal of Social Aspects of HIV/AIDS; Vol. 7, Iss. 2 August, 2010; pg:71-75
11.Abdul Karim Q, Abdool Karim SS, Singh B, Short R, Ngxongo S. Sero-prevalence of HIV infection in rural South Africa. AIDS; 1992 Dec; 6(12):1535-9.
12.Sekar et al. Recent trends in HIV prevalence in a remote setting of southern India: Insights into arranging HIV control policies.
13.Asia Pacific Coalition on Male Sexual Health (APOCOM), Mapping Transgender Groups, Organisation & Networks in South Asia, 2008; Available at http://www.msmasia.org.
14.WHO, Prevention and Treatment of HIV & Other Sexually Transmitted Infections Among Men Who Have Sex With Men & Transgender People:Recommendations for a Public Health Approach, June 2011; Available at http://www.who.int/hiv/pub/guidelines/msmguidelines2011/en/index.html, P.10.
15.HIV & Transgendered/Transexual Communities; Integragency Coalition on AIDS & Development, August 2011; Available at http://icad-cisd.com.
16.UNAIDS/WHO. Country Progress Report. UNGASS. India, March 2010. Available from: http://www.unaids.org/en/dataanalysis/monitoringcountryprogress/2010progressreportssubmittedbycountries/india_2010_country_progess_report_en.pdf
17.Vlassoff C, Weiss MG, Rao S, Ali F, Prentice T. HIV-related stigma in rural and tribal communities of Maharashtra, India.
Article citation:
Trivedi Grishma, Nerurkar AB. Seroprevalence of HIV infection in a rural tertiary care teaching hospital of South Gujarat from January To December 2012. J Pharm Biomed Sci 2014;04(04):356-360.Available at www.jpbms.info.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Trivedi Grishma, Nerurkar AB. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Niraj K Biswas1,*,Parimal H Patel2,Suresh Narayan Sharma3,Shankar Prakash4
Affiliation:-
1Assocaite professor,2Assistant Professor, Microbiology, GMERS Medical college, Valsad, Gujarat, India
3Professor,4Professor and HOD, Microbiology, PMCH, Patna, Bihar India
The name of the department(s) and institution(s) to which the work should be attributed:
1.GMERS Medical college, Valsad, Gujarat, India
2.PMCH, Patna, Bihar India
*To whom it corresponds:-
Dr Niraj K Biswas
Department of Microbiology, GMERS Medical college, Valsad, Gujarat, India
Contact no:+91-8758607152
Abstract:
Diarrhoeal diseases are a global public health problem causing considerable morbidity and mortality among infants and children especially in the developing countries. From the bacterial pathogen E.coli is constantly associated with it worldwide. Aim of the study to know prevalence of bacterial pathogen in diarrhoea in patients up to three year of age.
Materials and Methods: In duration of six month (Jan–June 2004) stool samples were collected from patients (up to 3 years of age) of diarrhoea and proceed by standard conventional method. Serological identification of isolated strains of E.Coli done at Central Research Institute Kasauli.
Results: stool samples of 125 patients were collected , in which male were more affected. E.coli isolated in 70.4% cases followed by Klebsiella sp., proteus sp., pseudomonas sp., shigella sp. and staphylococcus sp.. Isolated E.coli were of different serotype.
Conclusion: E. coli was predominant organism isolated. Serotype 055:B5 is commonest followed by 0127:B8 serotype in diarrhoea caused by E.coli.
Keywords: Diarrhoea, E.coli, serotype.
REFERENCES
1.Kamath KR. Fieldman RA. Sunder Rao, P SS & Webb, J.K.G. Infection and disease in group of south Indian families II General morbidity pattern in families and family member. Am.j of Epidemiology 89:375-383,1969.
2.Jadhav M. Report of work done on an investigation of diarhoea in infants and children ant CMC Vellore, ICMR 1965, New Delhi.
3.Kale VV, Lakshmi Iyer, & Jain MK. Shigellosis in infants and children J. Post.Grad. Med. Oct 1982; 28(4): 206-209,
4.Paveri KM. Infantile diarrhoea. Ind.J. Child health. 1952;1:418.
5.Sood S. Ind.J. Child health, 12:727,1963.
6.Sharma, S.K. Talija , R.K. Manjrekar, P.S. and Kant K.W. Ind. Paed. 1967;5:154-160.
7.Bhat PP & Mayers RM. Ind.J.Micro 1961;1:17.
8.Naruka,B.S Sharma,U. saxena,S.Aggarwal,S.S.& Sharma,M.L. Ind J.Path & Bact.Vol.13,No-6,451-455,1976.
9.Ordway NK. But Org. Mond. Sante, Bull. WHO 23: 73-101,1960.
10.Pande,R.C ,Rajvanshi, V.S and Malhotra,TN. Ind J. Path & Bact. Jan 1973;16:31.
11.Naidu,L: Study of infantile diarrhoea. Ind.J.Med.Res. 1962;50:613.
12.Tazir MA, Shrivastava KK, Khannisa SK, Aggrawal RC, Clinico-Bacteriological study of acute Gastroenteritis. Ind.J of Patho & Micro. Dec1978;85.
13.Behera SK, Mahapatra. Bacteriological study of diarrhoea in infants & childhood Ind.Paed. 1979;16:5.
14.Nath P,Bajpai PC. Amanullah, Khan AM. Ind.J.Path and Bact.1973; 16:37-41.
15.Shrivastava JR, Verma B,Garg BK. Acute diarrhoea in childhood. A clinical and bacteriological study. Ind.J.of Paed. 1968;35:217.
16.Basu B, Bhattacharya SR. Enterotoxigenicity and serotyping of E.Coli isolated from infantile gastroenteritis pt in Pune. Ind.J. Med. Res. 1983;Dec 78: 19-21.
17.Joseph Theresa. Bacteriology of diarrhoea in infants and children.Ind.J. of Micro. July-Sep.1980;20:3.
18.Sen Gupta SR,Sharma KD. Ind .J. Path and Bact. 1967;10:304.
19.Pande RC. Bacteriology of infantile diarrhoea and Gastroenteritis in Allahabad. Ind.J.Path & Micro. 1976;19:169.
20.Manorma Dev, Bhagwala R.A, Shriniwas & Singh,Maherbhan.Klebsiella pneumonia as the possible cause of an outbreak of diarrhoea in a neonatal special care unit. Ind.J.Med. March 1980;Res.71:359-362.
21.Arora MM, Pohowalla JN. Diarrhoea in infancy and childhood, Ind.J.Child Health. 1955;4:559.
22.Romas Alvarez M and Olate J, Diarrhoeal disease of children. The occurrence of enteropathogenic viruses and bacteria.Amer.J.Dis.child 1964;107:218.
23.Dmitrieva N, Zakaryan L.M. Staphylococcus in intestinal infection in infants.Abstracted in Excepta Medica (Paed), 1964;18:777.
24.Manchanda SS, Arora NN. Acute gastroenteritis in infants and young children with special reference to some etiological factors. Ind.J.Paed.1958;25:539.
25.Ahmed R, Bopp C. Phage typing scheme for Esch. Coli 0157:H7. J.Infect.Dis. 1987;155:806-809.
Article citation:
Biswas NK,Patel PH,Sharma NS,Prakash S. Study of prevalence of Bacterial pathogen as causative agent of diarrhoea in 0-3 years patients attending a Tertiary care Hospital Patna, Bihar,India. J Pharm Biomed Sci 2014; 04(03): 371-374. Available at www.jpbms.info.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Original article
Khetri R1,*, Dugar D2, Tim H T3
Affiliation:-
1Associate professor, 2Assistant professor, 3 2nd year P.G Student, Department of General surgery, Hi-Tech Medical college and Hospital, Bhubaneswar, Odisha, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of General surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
*To whom it corresponds:
Dr. DHARMENDRA DUGAR.
Assistant professor,
Department of surgery, Hi Tech Medical College & hospital; Bhubaneswar, Odisha, India
Mobile no.09437091846
Abstract:
Background: Little efforts have been done to know if Background: Health care associated infections are frequent in surgical wards and represent a high burden on patients and hospital in terms of morbidity, mortality, prolonged length of hospital stay and additional costs. In this study we analyse organism found at incision site intraoperatively and its relation with post-operative wound infection.
Material & method: We conducted a prospective hospital based study of 114 patients who underwent elective surgery. Patients were selected considering the inclusion and exclusion criteria. Two groups were considered–clean wounds (Group A) and clean contaminated wounds (Group B). Samples were taken from two sites–operative field and subcutaneous plane. Post-operative samples were collected from wound showing signs of infection.
Results: Most common organism grown in Group A in decreasing order was Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella and E. coli. Group B has Staphylococcus aureus, E. coli being the most common followed by Pseudomonas aeruginosa, Klebsiella. In post-operative period 4 patients from Group A and the same number of patients in Group B had serous/ purulent discharge. Out of 8/114 cases of surgical site infections only once the intra-operative site organism and post-procedure infection site organism was found to be same.
Conclusion: Although endogenous bacteria is responsible in most cases of SSI but intra-operative surgical site bacteria and post-operative SSI culture may be different. Hence taking suture site swab for culture in all surgical patients is not recommended for clean or clean contaminated wounds.
Keywords: Surgical Site Infection [SSI];Centres of Disease Control and prevention (CDC);Methicillin Resistant Staphylococcus Aureus [MRSA].
REFERENCES
1.Nichols RL. Post operative infections in the age of drug- resistant gram-positive bacteria. Am J Med. 1998;104:11S-16S.
2.Mshana SE, Kamugisha E, Mirambo M, Chakraborty T, Lyamuya EF. Prevalence of multiresistant gram-negative organisms in a tertiary hospital in Mwanza, Tanzania. BMC Res Notes. 2009 Mar 26;2:49.doi:10.1186/1756-0500-2-49.
3.Levy, S.B. (1998). The challenge of antibiotic resistance. Scientific American 278, 46-53
4.Larson E Community factors in the development of antibiotic resistance. Annu Rev Public Health. 2007;28:435-47.
5.Rao GG Risk factors for the spread of antibiotic- resistant bacteria. Drugs. 1998 Mar; 55(3):323-30.
6.Wong ES. Surgical site infection. In: Mayhall CG, editor. Hospital Epidemiology and Infection Control. Philadelphia: Lippincott Williams & Wilkins; 2004.p.287-310.
7.Sehulster L, Chinn RYW. Guidelines for environmental infection control in healthcare facilities. MMWR.2003;52(RR10):1-42.
8.Streifel AJ. Design and maintenance of hospital ventilation systems and the prevention of airborne nosocomial infections. In: Mayhall CG, editor. Hospital Epidemiology and Infection Control. Baltimore: Williams & Wilkins; 2004:1577-1589.
9.A Ramesh, R Dharini. Surgical site infections in a teaching hospital. Clinico-microbiological and Epidemiological Profile, VMMCH, Puducherry. Int J Biol Med Res.2012; 3(3); 2050-2053.
10.SP Lilani, N Jangale, A Chowdhary, GB Daver. Surgical site infection in clean and clean-contaminated cases. 2005; 23(4): 249-252.
11.JyotiSonawane, N Kamath, Swaminathan, Bacteriological Profile and their Antibiogram in a tertiary hospital in Mumbai, Bombay Hospital Journal. 2010; 52(3): 358-361.
12.Rasnake MS., Dooley DP. Culture-negative surgical site infections. Surg Infect (Larchmt). 2006 Dec; 7(6):555-65.
Article citation:
Khetri R, Dugar D, Tim HT. Surgical site infection and its relation to Intra operative suture site organisms. J Pharm Biomed Sci 2014; 04(04): 338-342. Available at www.jpbms.info.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.