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Research article
Nuha M.E. Agabna1 , Sania A.I. Shaddad2,*, A.K. Mudathir3
Affiliation:-
1 Department of Pharmacology, Faculty of Pharmacy University of Khartoum, Sudan
2 Deptartment of Medicine (Pharmacology), Faculty of Medicine University of Khartoum, Sudan
3 Department of Pharmacogonosy, Faculty of Pharmacy University of Khartoum, Sudan
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Pharmacology, Faculty of Pharmacy University of Khartoum, Sudan
*To whom it corresponds:-
Dr Nuha ME Agabna
Department of Pharmacology), Faculty of Pharmacy University of Khartoum, Sudan
Contact no:-++249912564229
Abstract
Lawsonia inermis, family Lythraceae (henna) is used traditionally to manage many conditions. This study evaluated the effects of 500 and 1000g/Kg dose of ethanolic seeds extracts on haematologic parameters, serum electrolytes, urea, creatinine, liver enzymes, plasma proteins, lipids profile, and random blood glucose.
Method: A single oral dose of 500mg and 1000mg /Kg was administered to two groups of mice to study acute toxicity. The extract was administered orally daily for 30 days to two groups of five animals each; one receiving 500mg/Kg and the other 1000mg/kg, a third group was control given normal saline 10ml/Kg. Blood samples were taken at days 0, 15 and 30 and analyzed for complete haemogram, kidney and liver function. By the end of the experiment animals were sacrificed, examined post mortally, histological slides of vital organs prepared to study the effects.
Results: The acute dose of 500mg and 1000mg /Kg caused no death in animals after 24 hours, and no signs of change in feeding, behavior, diarrhea or loss of fur were observed.
WBC, RBC haemoglobin and platelets count were minimally affected. Serum Na and K were not deviated from control, and so for creatinine and urea. Liver enzymes were not affected by chronic administration of henna extract. However a slight but significant elevation in AST was recorded in the high dose group. Plasma proteins were not affected. The lipids profile was similar to control group. Random blood glucose was same as the control group (within the limits). Post mortem examination showed no signs of toxicity. Observing feeding and animals behavior no differences were seen.
Conclusion: The ethanolic extract of L. inermis seeds at the studied doses is safe with no detrimental effects.
Keywords: Lawsonia inermis, safety, haematology, renal function, liver function.
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Article citation:
Nuha Agabna ME, Sania Shaddad AI, Mudathir AK. Safety of Lawsonia inermis ethanolic seeds extract. J Pharm Biomed Sci. 2014; 04(43):303-309. Available at www.jpbms.info.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Nuha Agabna ME, Sania Shaddad AI, Mudathir AK. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.