DocumentsDate added
Research Article
Tatapudi Padma SK1,*.,K.R.L.Surya kirani., M.D2
Affiliation:-
1,Assistant Professor, Department of Microbiology, Gayatri Vidya Parishad Institute of Health Care and Medical Technology,Visakhapatnam-530048, Andhra Pradesh, India
2Professor, Department of Microbiology, Rangaraya Medical College, Kakinada, Andhra Pradesh, India
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Microbiology, Gayatri Vidya Parishad Institute of Health Care and Medical Technology,Visakhapatnam-530048, Andhra Pradesh, India
2.Department of Microbiology, Rangaraya Medical College, Kakinada, Andhra Pradesh, India
Address reprint requests to
* Tatapudi Padma Satya Kumari.,MD.
Door No: 14-3/3(8), First Floor-3, S.V.S. Residency, T.I.C.point, Arilova, Visakhapatnam-530040
Article citation:
Tatapudi Padma S K ,Kirani KRLS. Sero diagnosis and clinical profile of dengue fever. J Pharm Biomed Sci 2014; 04(07):645-649. Available at www.jpbms.info
ABSTRACT
Background: Dengue is endemic in all countries except Europe. All ages and both sexes are susceptible to dengue fever (DF) occurring between June and November. Prompt, early diagnosis & treatment are essential to prevent the complications like Dengue Haemorrhagic Fever/Dengue Shock Syndrome (DHF/DSS) & minimize mortality rate.
Aim: Screening for Dengue IgM & IgG antibodies in clinically diagnosed/suspected dengue cases and to compare with clinical features. Screening for Dengue IgM antibodies in fever cases which are not clinically suspected as dengue evaluated for M.P and Widal test to detect cross reactivity/missed cases.
Material & Methods: One Hundred Sera from individuals clinically diagnosed/suspected as Dengue (study group1) and Sera for Widal and smears for Malarial parasite were collected from 100 fever cases not suspected as Dengue (study group 2). Sera analyzed for serological diagnosis of dengue by IgM and IgG ELISA. Results: Out of 100 clinically diagnosed as dengue cases screened, for IgM & IgG Dengue, 5 were positive for IgM, 34 for IgG and 30 for both IgM & IgG. Bleeding tendencies were comparatively high in IgG positive group; presence of rash and headache was high in the combined positivity group. Gastrointestinal manifestations like nausea & vomiting were present in the entire groups tested positive for dengue. Among the study group 2, IgM seropositivity was 20%. In this group fever was the only common symptom.
Conclusion: The study confirms the need for detection of Dengue IgM antibodies in all acute febrile illness cases, as the epidemic is still continuing year after year.
KEYWORDS: Break bone fever; Convulsions by a demon; Dengue; Dengue fever; Dengue haemorrhagic fever.
Paper was presented in: Indian Association of Medical Microbiologists Andhra Pradesh State Chapter XIII Annual Conference, Sri Venkateswara Institute of Medical Sciences & Sri Venkateswara Medical college, TIRUPATI, on 06-02-2010.
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15.Srinivasa Murthy K. Clinico serological study of dengue in Rajahmundry. Paper presented at IAMM AP chapter – 2007.
16.Neeraja M, V Lakshmi, VD Teja, P Umabala, MV Subbalakshmi. Serodiagnosis of Dengue Virus infection in patients presenting to a tertiary care hospital. Indian Journal of Medical Microbiology. 2006; 24(4):280-282.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Copyright © 2014 Tatapudi Padma S K ,Kirani KRLS. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original Research article:
Chijioke A. Nsofor1, Ogbulie T.E1, Nsofor M Chibuzor2
Affiliation:-
1Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
2Department of Medical Laboratory Science, Madonna University, Elele, Nigeria
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
2. Department of Medical Laboratory Science, Madonna University, Elele, Nigeria
Address reprint requests to
* Dr. Chijioke A. Nsofor,
Department of Biotechnology, Federal University of Technology,Owerri, Nigeria
Article citation:
Nsofor CA, Ogbulie TE, Nsofor CM. Conjugation Efficiency of Human Antibiotic Resistant Escherichia coli Isolates from Different Regions of Nigeria. J Pharm Biomed Sci 2014; 04(07):638-644. Available at www.jpbms.info
ABSTRACT
Localization of antimicrobial resistance genes on mobile genetic elements such as broad-host range plasmids, transposons, and integrons facilitates the horizontal transfer of these genes among bacteria and provides a rapid means of dissemination at the molecular level. In this study, transfer of resistance plasmids from human antibiotic resistance Escherichia coli isolates to laboratory strain was carried out using conjugation experiments. Plasmid-free Rifampicin-resistant recipient (gene-hog DH10B) was used in all matings in order to have a selectable marker for selection against the donor. Resistance plasmids were extracted and separated by agarose gel electrophoresis for profiling. The conjugation efficiency of the isolates ranges from 1.0 x 10-6- 7.5 x 10-7 in isolates from the south-west region; 1.1 x 10-6- 9.2 x 10-7 in isolates from south-south region; 1.0 x 10-6- 8.2 x 10-7 in isolates from north-north region and 1.1 x 10-6- 7.9 x 10-7 in isolates from the southeast region of Nigeria. A total of 146 plasmids was detected with molecular sizes ranging from 1 to 120 KB. The conjugation procedure is efficient enough to obtain transconjugants with sufficient delivery plasmids and therefore provides a simple route for conducting gene disruptions in bacteria. The conjugation efficiency of the isolates showed a high rate of transfer of resistance plasmids from antibiotic resistance strains to susceptible strains which contribute to the dissemination of antibiotic resistance traits in bacteria.
KEYWORDS: Conjugation Efficiency; Antibiotic Resistance; Escherichia coli.
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19.Okoli IC, Okeudo NJ, Onwuchekwa CI (2002). New trends in antibiotic resistance among E. coli isolates from southern Nigeria. In book of abstracts for the 39th annual national congress, Nigerian Veterinary Medical Association, 2002 October 27- 31; Sokoto, Nigeria. p. 16.
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21.Paul, J. H., M. E. Frischer, and J. M. Thurmond. (1991). Gene transfer in marine water columnand sediment microcosms by natural plasmid transformation. Appl. Environ. Microbiol. 57:1509-1515.
22.Poole T, and Crippen T (2009) Conjugative plasmid transfer between Salmonella enterica Newport and Escherichia coli within the gastrointestinal tract of the lesser mealworm beetle, Alphitobius diaperinus(Coleopetra: Tenebrionidae).Journal of Poultry Science Association 88: 1553-1558.
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24.Sanchez GV, Master RN, Karlowsky JA, Bordon JM (2012). In vitro antimicrobial resistance of urinary Escherichia coli isolates among US outpaitients from 2000 to 2010, Antimicrob. Agents Chemother. 56(4): 2181 – 2183.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Copyright © 2014 Nsofor CA, Ogbulie TE, Nsofor CM. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research Article
Gulsheen Kaur Kochhar1,*,Prinka Shahi2,Shweta Advani3, Preetinder Singh4,Sumit Kaushal4,Tanu Nangia5
Affiliation:-
1Assistant Professor, Department of Pedodontics & Preventive Dentistry, Swami Devi Dyal Hospital & Dental College, Barwala, Panchkula, Haryana, India
2Assistant Professor,Department of Pedodontics & Preventive Dentistry. MM College of Dental Sciences and Research Centre, Ambala, Haryana, India
3Senior Faculty & Academic Advisor, Aim MDS
4Associate Professor, Department of Periodontics & Implantology, Swami Devi Dyal Hospital & Dental College, Barwala, Panchkula, Haryana, India
5Assistant Professor, Department of Pedodontics & Preventive Dentistry, MPCDC,Gwalior,India
The name of the department(s) and institution(s) to which the work should be attributed:
1.Department of Pedodontics & Preventive Dentistry, Swami Devi Dyal Hospital & Dental College, Barwala, Panchkula, Haryana, India
2. Department of Pedodontics & Preventive Dentistry, MM College of Dental Sciences and Research Centre, Ambala, Haryana, India
2. Department of Periodontics & Implantology, Swami Devi Dyal Hospital & Dental College, Barwala, Panchkula, Haryana, India
3. Department of Pedodontics & Preventive Dentistry, MPCDC, Gwalior, India
Authors contributions:
All the authors contributed equally to this paper.
Address reprint requests to
* Dr. Gulsheen Kaur Kochhar .
Assistant Professor,
Department of Pedodontics & Preventive Dentistry, Swami Devi Dyal Hospital & Dental College Barwala, Haryana
Article citation:
Kochhar GK,Shahi P,Advani S,Singh P, Kaushal S,Nangia T. Dermatoglyphics of dental caries and Periodontal diseases in Children of North India. J Pharm Biomed Sci 2014;04(07):658-663.Available at www.jpbms.info
ABSTRACT
To determine the correlation between dermatoglyphics and the infectious oral diseases and to estimate dermatoglyphic dependence of dental caries and periodontal diseases in children of North India. 99 children (12-14 years) were divided in two groups; Group A for Caries Assessment And Group B For Gingival And Periodontal Assessment. Dermatoglyphic patterns of all 10 palmar digits were recorded using Cummins and Midlo method. Frequency distribution for dermatoglyphic peculiarities data in 99 children for caries assessment and gingival and periodontal assessment were tabulated. ANOVA test was applied for significance. ANOVA test revealed a highly significant difference was observed between the three groups of caries with loops and a significant difference P value : <0.005 (whorls) in caries assessment group. Whereas it was observed that between the three groups of OHI with loops and a no significant difference. An increase in whorls and decrease in loops with increase in caries and decrease in loops and no significant relationship with whorls with increase in periodontal diseases.
KEYWORDS: Dermatoglyphics; Caries; Periodontal Diseases; Children.
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7.Joel S. Fingerprints of palms and soles: Evidence. 1st edition. Lippicott publications. Dermatoglyphic interpretation of dental caries and itscorrelation to salivary bacteria interactions: An in vivo study. A Sharma, R Somani. JISSPD 2009; 27(1): 17-21.
Copyright © 2014 Kochhar GK,Shahi P,Advani S,Singh P, Kaushal S,Nangia T. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Research article
Ahmed, Abdalhameed1,*, M. & Elhag.Wafa,1,PhD
Affiliation:-
1MSc. Student, Faculty of Medical Laboratory Sciences-AL-Neelain University, Khartoum, Sudan.
2Assistant professor, Microbiology department, Faculty of Medical Laboratory Sciences-AL-Neelain University, Khartoum, Sudan
The name of the department(s) and institution(s) to which the work should be attributed:
Faculty of Medical Laboratory Sciences-AL-Neelain University, Khartoum, Sudan
Authors contributions:
Study idea, design, practical, analysis, writing of manuscript and editing all by author 1 and the supervising of research, and revising of paper by author 2.
Address reprint requests to
* Ahmed, Abdalhameed,
Faculty of Medical Laboratory Sciences-AL-Neelain University, Khartoum, Sudan
Article citation:
Abdalhameed A & Wafa E. Frequency of Hepatitis A virus among food handlers. J Pharm Biomed Sci 2014;04(07):664-669. Available at www.jpbms.info
ABSTRACT
Objectives: The aim of this study was to determine the frequency of the hepatitis A virus (HAV) in food handlers in Khartoum state, Sudan during period March to June 2014.
Materials & methods: A total of ninety food handlers, were enrolled in this study. Out of them 85 (94.4 %) were males and 5 (5.6%) females, and their age ranged between 30 to 39 with mean age of 33 years.
Blood specimens were collected and examined by ELISA technique for the detection of hepatitis A IgM& IgG antibodies.
Results: IgM antibody was detected among 6(6.7%) food handlers, while 32(35.6%) food handlers had IgG antibody, and 2.0 (3.6%) were positive for both. Most of seropositive were males 6(6.7%) IgM & 30 (33.3%)IgG,
Conclusion: The frequency of HAV IgM& IgG among the Food handlers is high in the age group (40-49) & (20-29) respectively. Education for behaviors along with screening, vaccination, and appropriate treatment for hepatitis A is strongly recommended to control this persistent infectious source of hepatitis A in the community.
KEYWORDS: HAV; food handlers; ELISA technique.
Core tip: - A critical problem for food handlers is hepatitis A infections. Some of the risk factors associated with HAV infection include poor hygiene, poor sanitation, and food & water contamination. In fact, HAV infection through food handlers has increased worldwide.
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Copyright © 2014 Abdalhameed A & Wafa E. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Original rresearch article:
OluwafemiAdeleke Ojo1,*, BasiruOlaitan Ajiboye2, Babatunji Emmanuel Oyinloye3,Christopher Oloruntoba Akintayo4
Affiliation:-
1,2,3Department of Chemical Sciences, Biochemistry Unit, Afe Babalola University Ado-Ekiti, Ekiti State, Nigeria
4Department of Physiology, College of Medicine and Health Sciences, Afe Babalola University Ado-Ekiti, Ekiti State, Nigeria
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Chemical Sciences, Biochemistry Unit, Afe Babalola University Ado-Ekiti, Ekiti State, Nigeria
2.Department of Physiology, College of Medicine and Health Sciences, Afe Babalola University Ado-Ekiti, Ekiti State, Nigeria
Address reprint requests to
* Mr. O.A. Ojo,
Department of Chemical Sciences, Biochemistry Unit,
Afe Babalola University, Ado-Ekiti, Nigeria
Article citation:
Ojo OA, Ajiboye B, Oyinloye BE, Akintayo CO. Prophylactic effects of Ethanolic extract of Alstonia boonei Stem bark against DDVP-induced Toxicity in albino rats. J Pharm Biomed Sci 2014; 04(07):650-657. Available at www.jpbms.info
ABSTRACT
The prophylactic effect of ethanolic extract of Alstonia boonei(AB) stem bark on(2,2-dichlorovinyl dimethyl phosphate)DDVP-induced oxidative damage in male albino rats’ liver was investigated. Male Wistar rats were divided into control, DDVP and treatment groups. In the prophylactic experiment, AB, (200 and 400 mg/kg body weight) was administered by oral gavage for 21 days before exposure to DDVP. Lipid peroxidation (LPO), reduced glutathione (GSH) levels, catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were then determined in the liver and heart alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were monitored and histological examination was carried out. Results indicate that DDVP-induced rats had significantly increased relative weight of liver and heart when compared to controls. Treatment with AB at 200 and 400 mg/kg caused a significant reduction in the relative weight of the organs. In DDVP-induced rats, serum ALT and AST activities and levels of LPO were increased whereas hepatic and cardiac SOD, CAT and GPx were significantly decreased. Furthermore, histological alteration in the liver and aorta were observed in DDVP untreated rats and were ameliorated in DDVP-induced treated rats with AB. In conclusion, the extract possesses antioxidant and hepatoprotective properties that eliminate the deleterious effects of toxic metabolites of DDVP.
Key Words:
KEYWORDS: Antioxidant, DDVP; hepatoprotective; prophylactic; Alstonia boonei.
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The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
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