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Research Article
Tatapudi Padma SK1,*.,K.R.L.Surya kirani., M.D2
Affiliation:-
1,Assistant Professor, Department of Microbiology, Gayatri Vidya Parishad Institute of Health Care and Medical Technology,Visakhapatnam-530048, Andhra Pradesh, India
2Professor, Department of Microbiology, Rangaraya Medical College, Kakinada, Andhra Pradesh, India
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Microbiology, Gayatri Vidya Parishad Institute of Health Care and Medical Technology,Visakhapatnam-530048, Andhra Pradesh, India
2.Department of Microbiology, Rangaraya Medical College, Kakinada, Andhra Pradesh, India
Address reprint requests to
* Tatapudi Padma Satya Kumari.,MD.
Door No: 14-3/3(8), First Floor-3, S.V.S. Residency, T.I.C.point, Arilova, Visakhapatnam-530040
Article citation:
Tatapudi Padma S K ,Kirani KRLS. Sero diagnosis and clinical profile of dengue fever. J Pharm Biomed Sci 2014; 04(07):645-649. Available at www.jpbms.info
ABSTRACT
Background: Dengue is endemic in all countries except Europe. All ages and both sexes are susceptible to dengue fever (DF) occurring between June and November. Prompt, early diagnosis & treatment are essential to prevent the complications like Dengue Haemorrhagic Fever/Dengue Shock Syndrome (DHF/DSS) & minimize mortality rate.
Aim: Screening for Dengue IgM & IgG antibodies in clinically diagnosed/suspected dengue cases and to compare with clinical features. Screening for Dengue IgM antibodies in fever cases which are not clinically suspected as dengue evaluated for M.P and Widal test to detect cross reactivity/missed cases.
Material & Methods: One Hundred Sera from individuals clinically diagnosed/suspected as Dengue (study group1) and Sera for Widal and smears for Malarial parasite were collected from 100 fever cases not suspected as Dengue (study group 2). Sera analyzed for serological diagnosis of dengue by IgM and IgG ELISA. Results: Out of 100 clinically diagnosed as dengue cases screened, for IgM & IgG Dengue, 5 were positive for IgM, 34 for IgG and 30 for both IgM & IgG. Bleeding tendencies were comparatively high in IgG positive group; presence of rash and headache was high in the combined positivity group. Gastrointestinal manifestations like nausea & vomiting were present in the entire groups tested positive for dengue. Among the study group 2, IgM seropositivity was 20%. In this group fever was the only common symptom.
Conclusion: The study confirms the need for detection of Dengue IgM antibodies in all acute febrile illness cases, as the epidemic is still continuing year after year.
KEYWORDS: Break bone fever; Convulsions by a demon; Dengue; Dengue fever; Dengue haemorrhagic fever.
Paper was presented in: Indian Association of Medical Microbiologists Andhra Pradesh State Chapter XIII Annual Conference, Sri Venkateswara Institute of Medical Sciences & Sri Venkateswara Medical college, TIRUPATI, on 06-02-2010.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Copyright © 2014 Tatapudi Padma S K ,Kirani KRLS. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research Article
Gulsheen Kaur Kochhar1,*,Prinka Shahi2,Shweta Advani3, Preetinder Singh4,Sumit Kaushal4,Tanu Nangia5
Affiliation:-
1Assistant Professor, Department of Pedodontics & Preventive Dentistry, Swami Devi Dyal Hospital & Dental College, Barwala, Panchkula, Haryana, India
2Assistant Professor,Department of Pedodontics & Preventive Dentistry. MM College of Dental Sciences and Research Centre, Ambala, Haryana, India
3Senior Faculty & Academic Advisor, Aim MDS
4Associate Professor, Department of Periodontics & Implantology, Swami Devi Dyal Hospital & Dental College, Barwala, Panchkula, Haryana, India
5Assistant Professor, Department of Pedodontics & Preventive Dentistry, MPCDC,Gwalior,India
The name of the department(s) and institution(s) to which the work should be attributed:
1.Department of Pedodontics & Preventive Dentistry, Swami Devi Dyal Hospital & Dental College, Barwala, Panchkula, Haryana, India
2. Department of Pedodontics & Preventive Dentistry, MM College of Dental Sciences and Research Centre, Ambala, Haryana, India
2. Department of Periodontics & Implantology, Swami Devi Dyal Hospital & Dental College, Barwala, Panchkula, Haryana, India
3. Department of Pedodontics & Preventive Dentistry, MPCDC, Gwalior, India
Authors contributions:
All the authors contributed equally to this paper.
Address reprint requests to
* Dr. Gulsheen Kaur Kochhar .
Assistant Professor,
Department of Pedodontics & Preventive Dentistry, Swami Devi Dyal Hospital & Dental College Barwala, Haryana
Article citation:
Kochhar GK,Shahi P,Advani S,Singh P, Kaushal S,Nangia T. Dermatoglyphics of dental caries and Periodontal diseases in Children of North India. J Pharm Biomed Sci 2014;04(07):658-663.Available at www.jpbms.info
ABSTRACT
To determine the correlation between dermatoglyphics and the infectious oral diseases and to estimate dermatoglyphic dependence of dental caries and periodontal diseases in children of North India. 99 children (12-14 years) were divided in two groups; Group A for Caries Assessment And Group B For Gingival And Periodontal Assessment. Dermatoglyphic patterns of all 10 palmar digits were recorded using Cummins and Midlo method. Frequency distribution for dermatoglyphic peculiarities data in 99 children for caries assessment and gingival and periodontal assessment were tabulated. ANOVA test was applied for significance. ANOVA test revealed a highly significant difference was observed between the three groups of caries with loops and a significant difference P value : <0.005 (whorls) in caries assessment group. Whereas it was observed that between the three groups of OHI with loops and a no significant difference. An increase in whorls and decrease in loops with increase in caries and decrease in loops and no significant relationship with whorls with increase in periodontal diseases.
KEYWORDS: Dermatoglyphics; Caries; Periodontal Diseases; Children.
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7.Joel S. Fingerprints of palms and soles: Evidence. 1st edition. Lippicott publications. Dermatoglyphic interpretation of dental caries and itscorrelation to salivary bacteria interactions: An in vivo study. A Sharma, R Somani. JISSPD 2009; 27(1): 17-21.
Copyright © 2014 Kochhar GK,Shahi P,Advani S,Singh P, Kaushal S,Nangia T. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Research article
Dr Nagi Idris*
Affiliation:-
Faculty of Pharmacy, Omar Al-Mukhtar University Albayda, Libya
The name of the department(s) and institution(s) to which the work should be attributed:
Faculty of Pharmacy, Omar Al-Mukhtar University Albayda, Libya
Address reprint requests to
Dr Nagi Idris.
Faculty of Pharmacy,
Omar Al-Mukhtar University Albayda, Libya
Article citation:
Idris N. Clozapine but not Haloperidol Prevents and Reverses a Sub-chronic PCP-induced Cognitive Deficit in the Attentional Set-Shifting Task in the Rat. . J Pharm Biomed Sci 2014;04(07):623-632. Available at www.jpbms.info
ABSTRACT
Introduction: Cognitive impairment is a pervasive feature of schizophrenia, and is a major determinant of the functional disability that is characteristic of the disorder. Administration of N-methyl-D-aspartate (NMDA) receptor antagonists in rodents has been proposed as an animal model of cognitive dysfunction in this disorder. Evidence from both animal models and human studies implicates a dysfunction of NMDA receptor function may attribute to pathophysiology of schizophrenia. Objectives: This study was undertaken to investigate the ability of sub-chronic co-administration of clozapine and haloperidol to both prevent and attenuate the cognitive deficits induced by the NMDA receptor antagonist, phencyclidine (PCP) in the attentional set-shifting task (ASST). Methods: In the first test, female Sprague-Dawley rats were treated with saline, clozapine 5.0 mg/kg or haloperidol 0.05 mg/kg, 30 min
later followed by either saline or PCP 2.0mg/kg twice daily for 7 days, followed by 7 days drug free before tested in ASST task. For the second test, female Sprague-Dawley rats received either vehicle or PCP 2.0 mg/kg for 7 days followed by 7 days drug free. Then rats received clozapine 5.0 mg/kg, haloperidol 0.05 mg/kg or vehicle twice daily for 7 days and were tested 120 min following the last dose of antipsychotic in ASST task. Results: Sub-chronic PCP significantly (p<0.01) increased the number of trials to reach criterion in the EDS phase when compared to vehicle. Atypical antipsychotic, clozapine but not the classical agent, haloperidol, significantly prevented and improved the cognitive impairment induced by PCP in ASST task. Conclusions: These results suggest that antagonism of the consequences of reduced NMDA receptor function could contribute to the superior efficacy of atypical antipsychotic agents in improving cognition in schizophrenia. This cognitive deficit likely reflects clinically relevant and can be used to evaluate the antipsychotic potential of new compounds on cognitive symptoms of schizophrenia.
KEYWORDS: NMDA receptors; PCP-Phencyclidine; Clozapine; Haloperidol; Novel Object Recognition Task; Deficit; Rat; Schizophrenia.
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Copyright © 2014 Idris N. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Original Research article:
Chijioke A. Nsofor1, Ogbulie T.E1, Nsofor M Chibuzor2
Affiliation:-
1Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
2Department of Medical Laboratory Science, Madonna University, Elele, Nigeria
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
2. Department of Medical Laboratory Science, Madonna University, Elele, Nigeria
Address reprint requests to
* Dr. Chijioke A. Nsofor,
Department of Biotechnology, Federal University of Technology,Owerri, Nigeria
Article citation:
Nsofor CA, Ogbulie TE, Nsofor CM. Conjugation Efficiency of Human Antibiotic Resistant Escherichia coli Isolates from Different Regions of Nigeria. J Pharm Biomed Sci 2014; 04(07):638-644. Available at www.jpbms.info
ABSTRACT
Localization of antimicrobial resistance genes on mobile genetic elements such as broad-host range plasmids, transposons, and integrons facilitates the horizontal transfer of these genes among bacteria and provides a rapid means of dissemination at the molecular level. In this study, transfer of resistance plasmids from human antibiotic resistance Escherichia coli isolates to laboratory strain was carried out using conjugation experiments. Plasmid-free Rifampicin-resistant recipient (gene-hog DH10B) was used in all matings in order to have a selectable marker for selection against the donor. Resistance plasmids were extracted and separated by agarose gel electrophoresis for profiling. The conjugation efficiency of the isolates ranges from 1.0 x 10-6- 7.5 x 10-7 in isolates from the south-west region; 1.1 x 10-6- 9.2 x 10-7 in isolates from south-south region; 1.0 x 10-6- 8.2 x 10-7 in isolates from north-north region and 1.1 x 10-6- 7.9 x 10-7 in isolates from the southeast region of Nigeria. A total of 146 plasmids was detected with molecular sizes ranging from 1 to 120 KB. The conjugation procedure is efficient enough to obtain transconjugants with sufficient delivery plasmids and therefore provides a simple route for conducting gene disruptions in bacteria. The conjugation efficiency of the isolates showed a high rate of transfer of resistance plasmids from antibiotic resistance strains to susceptible strains which contribute to the dissemination of antibiotic resistance traits in bacteria.
KEYWORDS: Conjugation Efficiency; Antibiotic Resistance; Escherichia coli.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Copyright © 2014 Nsofor CA, Ogbulie TE, Nsofor CM. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research Article:
Ashima Badyal1,*.,MD,Rajesh Pandey2.,MD,Kuldeep Singh Sodhi3.,MD,Jasbir Singh4.,MD
Affiliation:-
1Badyal Ashima*, MD Biochemistry, Registrar, Department of Biochemistry, GMC, Jammu, J&K,India
2Pandey Rajesh, MD Biochemistry, Professor, Department of Biochemistry, MMIMSR, Mullana,Ambala, Haryana,India
3Sodhi Kuldeep S, MD Biochemistry, Professor, Department of Biochemistry, MMIMSR, Mullana, Ambala, Haryana, India
4Singh Jasbir, MD Biochemistry, Professor and Head, Department of Biochemistry, MMIMSR, Mullana, Ambala, Haryana, India
The name of the department(s) and institution(s) to which the work should be attributed:
1.Department of Biochemistry, GMC, Jammu, J&K,India
2.Department of Biochemistry, MMIMSR, Mullana, Ambala, Haryana,India
Address reprint requests to
Ashima Badyal.
214-A Shastri Nagar, Jammu, J&K State, India- 180004 or at badyal.ashima@gmail.com
Article citation:
Badyal A, Pandey R,Sodi KS,Singh J. Evaluation of Serum Magnesium in Patients with Complicated Type 2 Diabetes Mellitus. J Pharm Biomed Sci 2014; 04(07):596-599. Available at www.jpbms.info
ABSTRACT
Context: Hypomagnesemia has been implicated in adversely affecting diabetic complications.
Several studies have demonstrated a higher than expected frequency of magnesium deficiency in patients with diabetes mellitus. Lack of accurate screening methodology is one of the main problems in assessing total body magnesium status.
Aims: To compare serum magnesium levels in type 2 diabetic patients with complications with those of non-diabetic controls and to assess the relationship between these two.
Setting and Design: Prospective cross sectional study in rural setting of Haryana.
Material and Methods: Fifty healthy controls and 100 patients with type 2 diabetes mellitus with complications were recruited from Medicine OPD of MMIMSR, Mullana, Ambala (Haryana), and their serum glucose and serum magnesium levels were measured and compared.
Statistical analysis: By SPSS version 12 [SPSS v12 (SPSS Inc., Chicago, IL)].
Results: Serum magnesium levels are decreased in type 2 diabetes mellitus with complications.
Conclusion: The early determination of magnesium status should be implemented in clinical practice for the better management of complicated diabetes mellitus.
KEYWORDS: Complications; magnesium; type 2 diabetes mellitus.
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Copyright © 2014 Badyal A, Pandey R,Sodi KS,Singh J. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info