DocumentsDate added
Original Article
Mahadeo Mane1,*, C.M.Kiran1, T.Mohana Lakshmi2, E. Prabhakar Reddy3
Affiliation:-
¹Department of Pathology, 2Department of Microbiology,3Department of Biochemistry, Sri Lakshmi Narayana Institute of Medical Sciences, Puducherry, India
The name of the department(s) and institution(s) to which the work should be attributed:
Sri Lakshmi Narayana Institute of Medical Sciences, Puducherry, India
Address reprint requests to
* Dr. Mahadeo Mane,
Professor of Pathology,
Sri Lakshmi Narayana Institute of Medical Sciences,
Puducherry,India or at manepath@yahoo.com.
Article citation:
Mane M,Kiran CM,Lakshmi TM,Reddy EP. Storage and stability of Blood samples – changes in the haematological values. J Pharm Biomed Sci 2014; 04(08):685-687. Available at www.jpbms.info
ABSTRACT
Introduction: The problem of medical errors has recently received a red alert of attention. For clinical laboratory medicine, the most frequent errors occur in the pre and post analytical phases. In general haematological parameters have been measured from EDTA anticoagulant whole blood, shortly after drawing. The time exciting between the blood drawing and the measurement of hematological parameters and the preparation, staining of blood smears for blood cell morphology examination. Material and Methods: Blood samples from healthy volunteer 26 subjects (13 women and 13 Men), aged (18-60years), were collected at SLIMS in puducherry and samples obtained by the vein puncture method and stored into EDTA tubes the blood sample were aliquated and separated into two groups.
Results and Discussion: The quality of results will probably improve because standardized storage conditions reduce pre analytical variation hematological parameters affected by storage and temperature in a way that could influence clinical decisions our research reports are supporting to do away assay immediately after drawing of CBC and blood cell morphological examination in order to avoid the misinterpretation of results.
KEYWORDS: Anticoagulant; Biochemical; heamtological Investigations; Clinical laboratory; Whole blood; Heamglobin.
REFERENCES
1.De Baca M E, Gulati G, Kocher W, Schwarting R. Effects of storage of blood at room temperature on hematologic parameters measured on Sysmex XE-20100. Lab. Med. 2006;37(1):28.
2.Schalm O W, Jain N C, Carroll E J. Veterinary haematology, 3rd edn, Lea & Febiger, Philadelphia, 1975.
3.Cohle S D, Abdus S, Makkouri D E. Effects of storage on stability of haematological parameters. Am J Clin Pathol. 1981; 76:67-69.
4.Meyer D J, Harvey J W. Veterinary laboratory medicine interpretation and diagnosis. 2nd edn. Saunders, Philidelphia. 1998.
5.Wood BL, Andrews J, Miller S, Sabbath D E. Refrigerated storage improves the stability of complete blood count and automated differential . Am J Clin Pathol. 1999; 112:687-695.
6.Buttarello M. quality specification in haematology: the automated blood cell count. Clin Chem Acta. 2004; 346:45-54.
7.Bonini P, Plebani M. Ceriotti F, Rubboli F. Errors in laboratory medicine. Clin Chem. 2000.46,89-99.
8.Jenny R W, Jackson- Tarentino K Y. Causes of unsatisfactiory performance in proficiency testing. Clin Chem. 2000;46:89-99.
9.Stahl M, Lund E D, Brandslund I. Reasons for laboratory s inability to report results for requested analytical tests. Clin Chem. 1998; 44:2195-7.
10.Hirase Y, Makatuska H, kawai T, Horiguchi S and lkeda M. Stable blood cell counts after one-week storage at room temperature, Ho CH, Chan IH (1995). The influence of time of storage platelet number in platelet-rich plasma packed cell, mean platelet volume, haemoglobin concentration, age, and sex on platelet aggregation test. Ann Hematol.1992; 71(3):129-133.
11.Gulati GL,. Hyland LJ, Kocher W, Schwarting R. Changes in automated complete blood cell count and differential leukocyte count results induced complete blood cell count and differential leukocyte count results induced by storage of blood at room temperature.J.C.Med. 2009; 115:10-13
12.Vogelaar S A, Posthuma D, Kluff C. Blood sample stability at room temperature for counting red and white blood cells and platelets. Vascul Pharmacol.2002; 39(3):123-125.
13.Wood B L, Andrews J, Miller S, Sabath D E. Refrigerated storage improves the stability of the complete blood cell count and automated differential, Am .J.Clin . Pathol.1992;112(5):687.
14.Mahmoodi M, Hajizadeh M, Rashidinejad H, Asadikaram G, Khakasri M, Mirzaee M, Seyedi N, Rahnema A, Sayadi A. Survey of changes in complete blood count and red cell indices of whole blood incubated in vitro at different temperatures up to 48 hrs J. Ayub Med. Coli . Abbottabad.2006.18(1).
Copyright © 2014 Mane M, Kiran CM,Lakshmi TM,Reddy EP. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Research Article
Jayanta Kumar Nandi1,Sougata Kumar Burman2,*, Debasis Das3,Dipta Prasun Saha4, Sangita Pal5
Affiliation:-
1RMO cum Clinical Tutor, Dept of Obs & Gynae, North Bengal Medical College, Sushrutnagar, Darjeeling, India
2Assistant Professor, Dept of Obs & Gynae, College of Medicine & JNM Hospital, WBUHS, Kalyani, Nadia, India
3Associate Professor, Dept of Community Medicine, Malda Medical College, Malda, India
4Associate Professor, Dept of Obs & Gynae, North Bengal Medical College, Sushrutnagar, Darjeeling, India
5Sangita Pal, Medical Officer, Nadia District Hospital, Nadia, India
The name of the department(s) and institution(s) to which the work should be attributed:
1.Department of Obs & Gynae, North Bengal Medical College, Sushrutnagar, Darjeeling, India
2.Department of Obs & Gynae, College of Medicine & JNM Hospital, WBUHS, Kalyani, Nadia, India
3.Department of Community Medicine, Malda Medical College, Malda, India
Authors contributions:
All the authors contributed equally to this paper.
Address reprint requests to
* Dr Sougata Kumar Burman,
Assistant Professor,
College of Medicine & JNM Hospital, WBUHS, Kalyani, Nadia, India
ABSTRACT
Objective: To determine the influence of socio-economic and cultural factors on the high prevalence of teenage pregnancy in a rural area of West Bengal. Methods: This was a cross sectional, observational study conducted at Nadia District Hospital, Krishnagar, West Bengal,India between 02/02/2013 and 02/08/2013 with a set of interview questionnaire and discussion with 309 teenage mothers selected by systemic random sampling technique in post natal ward. Results: 22.8% of total deliveries were teenage pregnancy. Cause of teenage pregnancy was early marriage. In 92.34% cases marriage was arranged by parents. In 68.93% cases the decision of early marriage was taken by their parents due to poverty; 71.52% due to social pressure and 46.27% due to large family size. Of all teenage mothers, 70.2% were aware of contraceptive method, but 76.69% of teenage mothers chose to be pregnant soon after marriage due to social pressure. Unlike developed countries all teenage pregnancies were socially accepted and have got familial and social support. Conclusion: To reduce the number of teenage pregnancy and its complication improvement of socioeconomic conditions and implementation of legal age of marriage is important.
KEYWORDS: Teenage pregnancy; Socio-economic factors; influence; West Bengal.
REFERENCES
1.www.bbc.uk.co/newsbeat/26026335.
2.Mehra S, Agarwal D. Adolescent health determinants for pregnancy and child health outcome among the urban poor.Indian Pediatrics 2004 ; 41: 137- 45.
3.Towards adulthood: Exploring the sexual and reproductive health of adolescents in south Asia/edited by Sarah Bott et. al. ISBN92 4 156250.
4.E. Singh Adolescent Reproductive Health in south Asia; key issues and priorities of action. UNFPA 2003; 64:48-52.
5.WHO :Towards adulthood: exploring the sexual and reproductive health of adolescents in South Asia 2003 : World Health Organization, Geneva. Available from http//apps.who. int/reproductive health/publications/towards_adulthood/towards_adulthood.pdf
6.Press Note on poverty estimates ,2011-2012, GOI, Planning Commission, July 2013.
7.Wazak C, Thapa S, Davey J. Influence of gender norms on the reproductive health of adolescent in Nepal – perspective of youth .WHO, Geneva 2003.
8.Jin Dryden, Pregnancies more likely in teens who smoke, drink,and use drugs . journal of adolescent health, Washington University in sent Lucia, February 3 2011.
9.Treffers P E (2003) Teenage Pregnancy, a worldwide problem “ Nederlands tijdschrift voor geneeskun 147 (47) 2320-2325 PMID”.
10.UNICEF (2011) A League Table of Teenage Births in Rich Nations.
11.www.gultmacher.org(pdf) Gultmacher Institute, June 2013 Retrieved on 14/4/14.
12.Mayor S (2004)” Pregnancy and child birth are leading cause of death in teenage girls in developing countries “ BMJ 328(7449):1152.
13.Atwood S J ,Hussain J. ,Adolescence motherhood :priorities and next steps. J Fam Welfare 1997 ; 43: 8-14.
14.Pathak K B et al. Adolescent Motherhood Problems and Consequences. J. Fam Welfare 1993:39:17-23.
15.Bratati Banarjee et al. Teenage Pregnancy a Socially Inflicted Health Hazard; Indian J Community Med July 2009;34(3):227-231.
16.P Mukherjee, R N Chowdhury: Hospital Based Perinatal Outcomes and Complications in teenage pregnancy in India; J Health Popul Nutr. Oct 2010; 28(5):494-500.
17.Singh S, Darroch J E. Adolescent Pregnancy and Childbearing: levels and trends in developed countries. Family Planning Perspectives, 2000; 32(1):14-25.
18.Singh S; Adolescent Childbearing in Developing countries; a global review Stud fam Plann 1998; 29:117-136.
19.B Banarjee . Comments to: Indused abortion and traumatic stress: A preliminary comparison of American and Russian women, Med Sci Monit 2007;13:LE 11-2.
20.Elul B, Verma S et al. Unwanted Pregnancy and Induced Abortion in Rajasthan, India. A qualitative exploration: available from www.popcouncil.org/pdfs/ SA Report. pdf.
Article citation:
Nandi JK,Burman SK,Das D,Saha DP,Pal S. Socio-cultural factors influencing teenage pregnancy in rural West Bengal,India. J Pharm Biomed Sci 2014;04(08):670-673. Available at www.jpbms.info
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Original article
SmithaNayak1,*, VinodNayak2, G. Somu3, Shankar B2
Affiliation:-
1Assistant Professor, School of Management, Manipal University, Manipal, Karnataka, India
2Associate Professor, Department of Forensic Medicine, Kasturba Medical College, Manipal University, Manipal, Karnataka, India
3Professor, Department of Hospital Administration, Kasturba Medical College, Manipal University, Manipal, Karnataka,India
The name of the department(s) and institution(s) to which the work should be attributed:
Kasturba Medical College, Manipal University, Manipal, Karnataka, India
Address reprint requests to
Dr.SmithaNayak.
Assistant Professor, School of Management, Manipal University, Manipal, Karnataka,India
Article citation:
Nayak S, Nayak V, Somu G, Shankar B. Knowledge and attitude of nurses to biomedical waste management: A cross sectional study. J Pharm Biomed Sci 2014; 04(08):733-736. Available at www.jpbms.info
ABSTRACT
Introduction: The hospital sector has gone through a revolutionary change in the process of health care delivery and has emerged into a complex multidisciplinary system. This sector utilizes an array of items in the process of delivering health care and leaves behind unusable waste which is has to be handled and disposed with utmost care. Among all the stakeholders in the healthcare industry, the nurses spend most of their time with the patients than any other employee segment. This increases their risk exposure to the hazards present in hospital environment. Hence there is a need for the nurses to be well equipped with latest information, skills and practices in managing biomedical waste management.
Methodology: We surveyed 128 nurses at Kasturba Hospital, Manipal to explore their knowledge and attitude towards biomedical waste management. This research also explored the biomedical waste management practices adopted by the nursing staff at the hospital. The research instrument used is a structured questionnaire that has been statistically validated.
Results: Among 128 respondents who participated in the survey, most of the respondents had less than five years of experience in the healthcare sector. Most of the nurses were knowledgeable on issues pertaining to biomedical waste management. 87 percent of the respondents were knowledgeable about the practices and procedures and 76 percent displayed a positive attitude towards biomedical waste management.
KEYWORDS: Biomedical waste; Knowledge; attitude; nurses.
REFERENCES
1.Saraj Badgujar, Sanskriti Menon, Anil Kumar, Shyamala Mani.Biomedical Waste Management Scenario in Pune. Savonier and Abstract book in National Workshop. 2002-2003; 30.
2.Shakharkar B.M. Principals of Hospital Administration 1st Ed. New Delhi: Jaypee Publishers 1998.
3.Sahar et al Intervention program for nurses about health care waste management Research journal of Medicine and medical sciences ,Egypt. 2012;7(1):25-37.
4.Sgerwal B.C, Lakshmy. A, ManojJais, GeetaMenta. Theoretical awareness and Practical Compliance of BMW Waste Management among Health Care Personnel in a tertiary Care Hospital in New Delhi. Souvenir and Abstract Book in National Work Shop.Indore: 2004.
Copyright © 2014 Nayak S, Nayak V, Somu G,Shankar B. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patients and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Case Study:
Anand Kumar GS., MD, FIPP
Affiliation:-
Associate professor, Department of Anaesthesiology and Pain Medicine, Sree Balaji Medical College, Chennai,India
Consultant Pain Specialist, Be Well Hospital, Chennai Advanced Interventional Spine and Pain Centre, Chennai,India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Anaesthesiology and Pain Medicine, Sree Balaji Medical College, Chennai, India
Address reprint requests to
Dr. Anand Kumar GS, MD, FIPP
Associate professor, Department of Anaesthesiology and Pain Medicine, Sree Balaji Medical College, Chennai, India
Article citation:
Anand Kumar GS. Trigeminal Ganglion Radiofrequency Thermocoagulation (TN RFTC) For Trigeminal Neuralgia- A Case Series Study. J Pharm Biomed Sci. 2014; 04(08):694-697. Available at www.jpbms.info
ABSTRACT
Idiopathic trigeminal neuralgia is one of the commonest condition of cephalagia characterized by sharp shooting lancinating pain along the distribution of one of the division of trigeminal ganglion, the most commonly affected segment is maxillary (V2) and mandibular(V3), followed by ophthalmic(V3).The treatment options available are non-surgical conservative, Interventional management and surgical. Our objective of the study is to follow the cases which we have treated with an Interventional technique radiofrequency thermocoagulation (RFTC) in our institute. A total number of 11 cases followed for a period of four years after successful RFTC for trigeminal neuralgia. We found it is a good technique for long term pain relief with mild numbness to be the side effects and regarding safety wise it is very safe and no complications have been noticed if performed with cautions.
KEYWORDS: Trigeminal ganglion; trigeminal neuralgia; radiofrequency thermocoagulation.
REFERENCES
1.Janetta PJ. Microsurgery of cranial cross-compression. Clin Neurosurg. 1979;26:607-615.
2.Janetta PJ. Microsurgery of cranial nerve cross-compression. Clin Neurosurg. 1990;73:212-216.
3.BROWN JA, MACDANIEL MD, WEAVER MT: Percutaneous trigeminal nerve compression for treatment of trigeminal neuralgia: Results in 50 patients. Neurosurgery.1993;32:570-573.
4.Taha JM JR, Tew JM JR. A prospective 15-year follow up of 154 consecutive patients with trigeminal neuralgia treated by percutaneous sterotactice radiofrequency rhizotomy. J Neurosurg.1995; 83:989-993.
5.Gokalp HZ, Kanpolat Y, Turner B. Carotid-cavernous fistula following percutaneous trigeminal ganglion approach. Clin Neurol Neurosurg. 1980; 82:269-272.
6.LIU LH, Huang RH. The complications of radio frequency therapy for trigeminal neuralgia. Chin J Nerv Ment Dis (Chin). 2002;28:215-216.
7.International Headache Society Classification Subcommittee. The international classification of headache disorders: 2nd edition. Cephalgia. 2004; 24:9–160.
8.Headache Classification Committee of the International Headache Society. Classification and diagnostic criteria for headache disorders, cranial neuralgias and facial pain. Cephalalgia. 1988;8 (Supple 7):1–96.
9.McQuay H, Carroll D, Jadad AR, Wiffen P, Moore A. Anticonvulsant drugs for management of pain: a systematic review. BMJ. 1995;311:1047–1052.
10.Janetta P. Trigeminal neuralgia: treatment by microvascular decompression. In: Wilkins R, Regachary S, eds. Neur osurgery. New York: McGrawy-Hill; 1996:3961– 3968.
11.Lopez BC, Hamlyn PJ, Zakrzewska JM. Stereotactic radiosurgery for primary trigeminal neuralgia: state of the evidence and recommendations for future reports. J Neurol Neurosurg Psychiatry. 2004;75:1019–1024.
12.van Kleef M, Mekhail N, Van Zundert J. Editorial; Evidence based guidelines for interventional pain
medicine according to clinical diagnoses. Pain Pract. 2009;9: 247–251.
13.Lopez,B.C.,Hamlyn,P.J.&Zakrzewska,J.M.(2004)Systemic review of ablative neurosurgical techniques for the treatment of trigeminal neuralgia.Neurosurgey. 2002; 4,973-983.
14.Mullan S, & Lichtor T., Percutaneous microcompression of the trigeminal ganglion for trigeminal neuralgia.Journal of Neurosurgery.1983; 59,1007-1012.15.
15.Gauci,C.(2004).Manual of RF Techniques. Switzerland:Filivo Press.
Copyright © 2014 Anand Kumar GS. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info
Research article:
Shruti Jain*
Affiliation:-
Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh. 173234. India
The name of the department(s) and institution(s) to which the work should be attributed:
Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh. 173234. India
Address reprint requests to
Dr. Shruti Jain.
Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh. 173234. India
Article citation:
Ja in S., Implementation of Fuzzy System us ing O perational Transconductance Amplifier for ERK pathway of EGF/ Insulin leading to Cell Survival/ Death. J Pharm Biomed Sci. 2014;04(08):705-711. Available at www.jpbms.info
J Pharm Biomed Sci. 2014;04(08):705-711.
ABSTRACT
In this paper a well defined method for the design of ERK pathway for epidermal growth factor/ insulin using fuzzy system using operational transconductance amplifier was discussed. Fuzzy system includes fuzzification of the input variables, application of the fuzzy operator (AND or OR) in the antecedent, implication from the antecedent to the consequent, aggregation of the consequents across the rules, and defuzzfication. The fuzzy system (including all blocks) for estimating the cell survival/death using ERK pathway, exhibits a gain of 77.25 dB, input resistance with 44.13KΩ, output resistance with 5.163KΩ, CMRR with 73.84dB, slew rate with 0.6V/µsec and power dissipation with 0.197mW.
KEYWORDS: Epidermal growth factor (EGF)/ Insulin; Extracellular signal regulated kinase (ERK); operational transconductance amplifier; Fuzzy system; Electrical parameters.
REFERENCES
1.Jain S., Communication of signals and responses leading to cell survival / cell death using Engineered Regulatory Networks. PhD Thesis, Jaypee University of Information Technology, Solan, Himachal Pradesh, India, 2012.
2.Ullrich A.,Schlessinger J., Signal transduction by receptors with tyrosine kinase activity, Cell, 1990;61: 203-211.
3.Arteaga C., Targeting HER1/EGFR: a molecular approach to cancer therapy. Semin Oncol, 2003;30:314.
4.Jain S, Design and simulation of fuzzy membership functions for the fuzzification module of fuzzy system using operational amplifier, International Journal of Systems, Control and Communications (IJSCC). 2014;6(1):69-83.
5.Jain S., Design and Simulation of Fuzzy Implication Function of Fuzzy System Using Two Stage CMOS Operational Amplifier, International Journal of Emerging Technologies in Computational and Applied Sciences (IJETCAS), Feb 2014;7(2):150-155.
6.Berkan R.C., Trubatch S. L., Fuzzy System Design Principles, 1997.1st edition, Wiley-IEEE Press.
7.Drankov D., Hellendoorn H., Reinfrank M., An introduction to Fuzzy Control,1993. New York, Springer-Verlag.
8.John Yen,Reza Langari, Fuzzy Logic: intelligent Control and Information, 1998. United states ed edition, Prentice Hall.
9.E. Sanchez Sinenchio, J. Silva Martinez,CMOS transconductance amplifiers, architectures and active filters: A tutorial, IEEE Proc-Circuits Devices System.2000;147(1):3-12.
10.Allen P.E, holberg D.R., CMOS analog circuit design , 2011. International Student edition, Oxford.
11.MaSavisha A. P. halingam, Md. Mamun, Labonnah F. Rahman, Wan Mimi Diyana Wan Zaki, Design and Analysis of a Two Stage Operational Amplifier for High Gain and High Bandwidth,Australian Journal of Basic and Applied Sciences, 2012;6(7):247-254.
12.Patel P.D. , Shah K.A., Design of Low Power Two Stage CMOS Operational Amplifier,International Journal of Science and Research,2013;2(3):432-434.
13.Gayakwad, R.A., Op-Amps and Linear Integrated Circuits, 2002. 3rd ed., Prentice Hall of India Pvt. Ltd, New Delhi.
14.Sedra S Smith, Microelectronic circuits, 2004. 5the d. UK: Oxford University Press.
15.Minh Anh Nguyen, Pspice Tutorial, Class: Power Electronic 2 , By Colorado State University Student. 16.Rashid M.H., Introduction to PSICE using OrCAD for circuits and electronics, 2009. 3rd e dition, PHI.
Copyright © 2014 Jain S. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclosure forms provided by the authors are available with the full text of this article at jpbms.info