DocumentsDate added
Original article
Rajyalakshmi Gunti1* †.MD,Durga Rani Arava2.MD,K .VenkataRamana3,†.MBBS,DNB,DMRD
Affiliation:-
1Assistant Professor, Department of Microbiology, Rangaraya Medical College, GGH Campus, Kakinada, Andhrapradesh, India-533001.
†Consultant, Swathi Imaging and Diagnostics, Kakinada, Andhrapradesh, India-533001.
2Assistant professor, Department of Microbiology, Rangaraya Medical College, GGH Campus, Kakinada, Andhrapradesh, India-533001.
3Assosciate professor, Department of Radiology, KIMS, Amalapuram, Kakinada, Andhrapradesh, India-533001.
†Technical director and Consultant, Swathi Imaging and Diagnostics, Kakinada, Andhrapradesh, India-533001.
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, Rangaraya Medical College, GGH Campus, Kakinada, Andhrapradesh, India-533001.
Swathi Imaging and Diagnostics, Kakinada, Andhrapradesh, India-533001.
Address reprint requests to
Dr. G. RAJYALAKSHMI,MD
Assistant professor, Department of Microbiology, Rangaraya Medical College, GGH Campus, Kakinada, Andhrapradesh, India-533001
Article citation:
Gunti R, Arava DR, Ramana KV. Prevalence of extended spectrum β-Lactamases among Escherichia coli And Klebsiella Pneumoniae in and around Kakinada, Andhrapradesh, India. J Pharm Biomed Sci. 2014; 04(09):773-775. Available at www.jpbms.info
ABSTRACT
Extended spectrum β–lactamases (ESBLs) continue to be a major problem worldwide conferring resistance to the expanded spectrum cephalosporins. As no data is available in this area an attempt was made to know the prevalence of ESBL producers among Escherichia coli and Klebsiella pneumoniae in and around Kakinada. A total of 469 Escherichia coli and 80 Klebsiella pneumoniae were studied over a two year period from March 2012 to February 2014. ESBL production was identified in 73.1% of E.coli and 58.8% of K.pneumoniae. As a large number of isolates were found to be ESBL producers in the present study, continued monitoring of drug resistance is recommended for proper disease management.
KEYWORDS: Extended spectrum β–lactamases; Escherichia coli; Klebsiella pneumoniae.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
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Copyright © 2014 Gunti R, Arava DR, Ramana KV. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article:
Delgado, E.A.D.1, Fonseca JR., J.H.2, Gibertoni, F.2, Beretta, A.L.R.Z3,*
Affiliation:-
1Student-Specialization in Clinical Analysis University Center Hermínio Ometto (UNIARARAS) Araras, S.P.
2Masters Student - Graduate Program - Biomedical Sciences Center Hermínio Ometto (UNIARARAS), Araras, S.P.
3* Doctor-teacher of the Graduate Program in Biomedical Sciences, University Center Hermínio Ometto (UNIARARAS), Araras, SP, Brasil
The name of the department(s) and institution(s) to which the work should be attributed:
Microbiological Research Laboratory, Av. Dr. Maximiliano Baruto, 500 Jd. Universitário. 13607-339 - Araras – SP – Brasil
Address reprint requests to
Ana Laura Remédio Zeni Beretta
Microbiological Research Laboratory, Av. Dr. Maximiliano Baruto, 500 Jd. Universitário. 13607-339 - Araras – SP – Brasil
Article citation:
Delgado, E.A.D., Fonseca JR., J.H., Gibertoni, F., Beretta, A.L.R.Z. Biosafety and the tuberculosis in units of health. J Pharm Biomed Sci. 2014; 04(09):769-772. Available at www.jpbms.info
ABSTRACT
Tuberculosis is an infectious disease of worldwide extent, caused by Mycobacterium tuberculosis and its transmission has always been a frequent concern in healthcare services. With the growth of strains resistant to first-line antituberculosis drugs and the emergence of Acquired Immune Deficiency Syndrome have made this more intense concern.
Objectives: Through exploratory study of articles and books published in the databases LILACS, MEDLINE, thesis database, aimed to identify the risks of health professionals for the occurrence of pulmonary tuberculosis and demonstrate the behaviors of these biosafety professionals exposed to M. tuberculosis.
Results: Due to the large number of infected people and the high risk of movement of persons affected by the disease, biosecurity should function as an effective ally, through action plans and control in health care institutions.
Conclusion: Based on the data, the study suggests that health professionals are at risk because of the limitations found with regard to preventive measures, especially the delayed diagnosis of tuberculosis. It is extremely important to implement biosecurity measures in health facilities, as well as the investigation of pulmonary tuberculosis, since these professionals are unaware of the occupational risks.
KEYWORDS: Pulmonary tuberculosis, health professionals, occupational risk; Biosafety.
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Copyright © 2014 Delgado, E.A.D., Fonseca JR., J.H., Gibertoni, F., Beretta, A.L.R.Z. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Original article:
Ofem E. Ofem1, Victor U. Nna1,*, Victor O. Oka1, Archibong N. Archibong1, Stella C. Bassey2
Affiliation:-
1Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria
2Department of Nutrition and Dietetics, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria
The name of the department(s) and institution(s) to which the work should be attributed:
Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria
Address reprint requests to
Victor U. Nna
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria
Article citation:
Ofem OE, Nna VU, Oka VO, Archibong AN, Bassey SC. Vitamin C and E Supplementation Reverses Alterations in Haematological Indices Induced by High Salt Loading in Rats. J Pharm Biomed Sci. 2014; 04(09):763-768. Available at www.jpbms.info
ABSTRACT
Background: High salt intake has been linked with deleterious effects on the body, especially on arterial blood pressure and red blood cell hemolysis. Since vitamins C & E are acclaimed anti-oxidant vitamins, their ameliorating effects on the menace of high salt loading is suggestive. This study therefore seeks to investigate the impact of vitamin C & E supplementation on haematological indices in high salt loaded rats.
Methodology: Twenty four male albino Wistar rats weighing 180 - 200 g were divided into 4 groups (n = 6) as follows; control (fed with normal rat chow + drinking water), control + vitamin C and E group (10mg/100g bw vitamin C and 83.8mg/100g bw vitamin E, in addition to control diet), salt – fed (SF) group (8% NaCl diet + 1% NaCl drinking water), salt – fed treated (SF + Vitamin C & E) group (8% NaCl diet + 1% drinking water in addition to 10mg/100g bw vitamin C and 83.8mg/100g bw vitamin E). After 42 days of treatment, the animals were sacrificed and blood samples collected through cardiac puncture for measurement of haematological indices.
Results: Red blood cell (RBC) count, white blood cell (WBC) count, packed cell volume (PCV), mean corpuscular haemoglobin concentration (MCHC), red cell distribution wide-standard deviation (RDW-SD) and red cell distribution wide-coefficient of variation (RDW-CV) were significantly (P<0.05) higher in SF group compared with control. RDW-SD and RDW-CV were significantly (P<0.05) lower in SF + Vitamin C & E group compared with SF group. Haemoglobin concentration was significantly (P<0.05) lower in the SF + Vitamin C & E group compared with SF group. Neutrophils count was significantly (P<0.05) increased in SF + Vitamin C & E group compared with control, Vitamin C & E and SF group.
Conclusion: Vitamin C & E supplementation reversed the changes in RBC and RBC indices occasioned by high salt loading, but potentiated the effects of high salt loading on WBC and platelet indices in rats.
KEYWORDS: Platelets; red blood cells; salt; vitamin C; vitamin E; white blood cells.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Original article
Mervat A. Ahmed1, Mona A. Mohamed2,*, Maha G. Soliman1,Yasmine H. Abd El-Naby3
Affiliation:-
1Zoology Department, 2Biochemistry division, 3Chemistry Department, Faculty of Science, Al-Azhar University for Girls, Egypt
3Military Clinics, Cairo, Egypt
The name of the department(s) and institution(s) to which the work should be attributed:
Faculty of Science, Al-Azhar University for Girls, Egypt
Address reprint requests to
Mona A. Mohamed.
Biochemistry division, Faculty of Science, Al-Azhar University for Girls, Egypt
Article citation:
Ahmed MA, Mohamed MA, Soliman MG, Abd Al-Naby YH. Monitoring The Expression Of Some Cell Adhesion Molecules And Serum Levels Of IL-6 And TNF-α In Irradiated Rats Treated With Wheat Germ Oil, Zinc And Bone Marrow. J Pharm Biomed Sci. 2014; 04(09):755-762. Available at www.jpbms.info
ABSTRACT
Background: Both radiotherapy and chemotherapy exert damaging effects on normal tissues in cancer patients and, consequently, induce an immune response in these tissues. Ionizing radiation in interaction with living cells causes a variety of changes depending on type of exposure, absorbed dose, duration of exposure, interval after exposure and also susceptibility of tissues. Aim: The present work was designed to evaluate the amelioration of serum IL-6 and TNF-α, in addition to expression of ICAM-1 and VCAM-1 genes in hepatic tissue of rats exposed to gamma radiation and treated with wheat germ oil and/or zinc sulfate individually or in combination with bone marrow transplanted cells.
Results: Exposure of rats to 5 Gy γ-rays (single dose) caused elevation in serum levels of MDA and IL-6, in addition to hepatic mRNA levels of ICAM-1 and VCAM-1 (62.2, 1.84-, 3.2- and 5.2- fold, respectively). On the other hand, levels of reduced glutathione and TNF-α were significantly lowered. Although administration of WG and Zn (either individually or in combination) improved the studied serum parameters, their levels were significantly different from control values. While hepatic mRNA levels of the adhesion molecules returned to near the control levels. Conclusion: Wheat germ oil and zinc in combination with bone marrow cells can be used as radioprotectors to improve the inflammatory status and disturbances in immune response induced by radiation.
Keywords:
KEYWORDS: Adhesion molecules; Inflammation; IL-6; Radiation; TNF-α.
Competing interest / Conflict of interest: None
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Copyright © 2014 Ahmed MA, Mohamed MA, Soliman MG, Abd Al-Naby YH. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interest / Conflict of interest: None
Original article
Niraj Kumar Biswas1,*,Parimal H Patel2,Hitesh R Ahir3,Purvi H Kothadia3,Alka B Nerurkar4
Affiliation:-
1Associate Professor, 2Assistant Professor, 3Tutor,4Professor and Head, Department of Microbiology, GMERS Medical College, Valsad, Gujarat, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, GMERS Medical College, Valsad,Gujarat, India
Address reprint requests to
Dr.Niraj KUmar Biswas.
Associate Professor, Department of Microbiology, GMERS Medical College, Valsad, Gujarat, India
Article citation:
Biswas NK,Patel PH,Ahir HR,Kothadia PH,Nerurkar AB. Bacteriological profile of Urinary tract infection and its antibiotic susceptibility at tertiary care Hospital, Valsad, Gujarat,India. J Pharm Biomed Sci. 2014; 04(09):751-754. Available at www.jpbms.info
ABSTRACT
Objective:This study was done to find out the common bacteria causing UTI and to determine the antibiotic susceptibility pattern of the urinary pathogens in patients attending a tertiary care hospital in Valsad, Gujarat.
Material and Methods: In duration of January to July 2014, 179 urine samples were collected from patients of suspected UTI, Collected samples were inoculated on sheep Blood agar, Mac Conkey agar and Nutrient agar and incubated at 37°C by using standard method and antibiotic susceptibility test were performed by Kirby Bauer disc diffusion method.
Results: 76(42.45%) samples were found to be positive in a culture in which 60 (78.95%) were gram negative, 13(17.10%) were Gram positive and 3 (3.95%) were candida spp. Maximum number of organisms isolated were E.coli, followed by Klebsiella sp., Pseudomonas sp. Enterococcus sp, Candida and other organisms. Isolated organisms show more resistance to commonly used antibiotics.
Conclusion: Gram negative bacilli are the commonly isolated organisms in UTI and E.coli is most common of them. An isolated organism shows more resistance to commonly used antibiotics.
KEYWORDS: UTI; Antibiotic susceptibility; urinary pathogens.
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Copyright © 2014 Biswas NK,Patel PH,Ahir HR,Kothadia PH,Nerurkar AB. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interest / Conflict of interest: None