DocumentsDate added
Original article
Devinder Singh Negi1, Pankaj Shrivastava2, Smruti Prava Das1,*
Affiliation:-
1Department of Chemistry, Ravenshaw University, Cuttack - 753003, Odisha, India
2DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar - 470001, Madhya Pradesh, India
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Chemistry, Ravenshaw University, Cuttack - 753003, Odisha, India
2. DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar - 470001, Madhya Pradesh, India
Address reprint requests to
Dr.Smruti Prava Das.
Department of Chemistry, Ravenshaw University, Cuttack - 753003, Odisha, India
Article citation:
Negi DS, Shrivastava P, Das SP. Microsatellite markers alleles analysis in 12 cases of paternity establishment by DNA Profiling. J Pharm Biomed Sci. 2014; 04(12):1057-1071. Available at www.jpbms.info
ABSTRACT
The paternity is established by examination of inherited alleles in the questioned child from the reference parent samples. The alleles for all examined microsatellite loci unambiguously assigned to the questioned child as maternal and paternal alleles. In this study, we have examined 11 family trios and 1 family with 4 trios and convincingly established the paternity in 12 cases by DNA profiling. This study present an extensive analysis of number of possible alleles, homozygous and heterozygous alleles, inheritance pattern of maternal and paternal alleles in child, paternity index and probability of paternity in all the 12 cases. The total numbers of alleles in 15 microsatellite loci are 182. The average percent numbers of homozygous and heterozygous loci were 21.63 and 78.37 respectively. The likelihood of first allele in the child inherited from mother and fathers were 6.8 and 8.2 respectively. The average cumulative paternity index and probability of paternity were 1124182187 and 0.999999859 respectively in all the 12 cases. The analysis of STR alleles of microsatellite markers inferred the high heterozygosity, around equal chance that first inherited allele is from either parent, and high average values of cumulative paternity index and probability of paternity. The sample size of 12 cases wherein 15 trio are examined exhibited the robustness, efficiency and accuracy of markers with high diverse allele pool for establishment of paternity. The study showed the application of allele frequency database of Indian population, probability value and statistical methods in paternity establishment cases.
KEYWORDS: microsatellite, alleles; homozygosity; heterozygosity; paternity index; probability.
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4.Negi DS, Das SP. Quantitative and qualitative chemical extraction of Deoxyribo Nucleic Acid DNA from human cell organelles. Res J Chem Sci. 2014;4(8):75-81.
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8.Negi DS, Alam M, Bhavani SA, Nagaraju J. Multi-step microsatellite mutation in maternally transmitted locus D13S317: A case of maternal allele mismatch in the child. Int J of Legal Med. 2006;120:286-92.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Copyright © 2014 Negi DS, Shrivastava P, Das SP. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Hitesh R Ahir*,Niraj Kumar Biswas, Parimal H Patel, Mehul R Patel
Affiliation:-
Department of Microbiology, GMERS Medical College & Hospital, Valsad, Gujarat, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, GMERS Medical College & Hospital, Valsad, Gujarat, India
Address reprint requests to
Hitesh R Ahir,
Department of Microbiology GMERS Medical College & Hospital, Valsad, Gujarat, India
Article citation:
Ahir HR, Bisvas NK, Patel PH, Patel MR. Prevalence of Pseudomonas infection and its antibiotic susceptibility pattern isolated from various clinical samples at tertiary care hospital, Valsad, Gujarat. J Pharm Biomed Sci. 2014;04(12):1039-1041. Available at www.jpbms.info
ABSTRACT
Objectives: Pseudomonas is one of the important bacterial pathogens isolated from various clinical samples. Several studies indicate that resistance patterns are increasing nowadays. The present study was done to assess the Prevalence of Pseudomonas infection and antibiotic susceptibility pattern of pseudomonas isolated from various clinical samples.
Materials and Methods: A total of 697 samples were cultured out of them 71 pseudomonas isolates from different clinical samples, e.g. urine, pus.csf, sputum, and blood were tested for antibiotic susceptibility pattern using modified disk diffusion method as per Clinical and Laboratory Standards Institute guideline (CLSI)
Results: Prevalence of Pseudomonas infection is 10.18% detected at GMERS medical college and Hospital, Valsad, Gujarat, India. Major part of Pseudomonas isolates were detected from wounds.
Conclusion: Prevalence of Pseudomonas is higher in surgical patients and Multi-drug resistant isolates are also present in this institute.
KEYWORDS: Pseudomonas, Kirbey bour disk diffusion method, Antibiotic susceptibility pattern.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Copyright © 2014 Ahir HR, Biswas NK, Patel PH, Patel MR. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Review article
Rahul Kumar Mishr1,*, Jitender Dhillon2, Rajesh Pandey3, K. S. Sodhi3, Jasbir Singh3
Affiliation:-
1 MSc MLT (Intern), 2MD Student, 3Professor (MD)
Department of Biochemistry, MMIMSR, Mullana, Ambala, Haryana, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Biochemistry, MMIMSR, Mullana, Ambala, Haryana, India
Address reprint requests to
Rahul Kumar Mishr.
MSc MLT (Intern), Department of Biochemistry, MMIMSR, Mullana, Ambala, Haryana, India., India
Article citation:
Mishr RK, Dhillon J, Pandey R, Sodhi KS, Singh J. Quantum Dots In Medical Science. J Pharm Biomed Sci. 2014; 04(12):1042-1049. Available at www.jpbms.info
ABSTRACT:
Quantum dots (QDs) are tiny semiconductor crystals of size 1-10 nanometres made up of compounds from group II to VI and III to V. The exceptional photophysical properties of QDs, particularly photostability and emission as a function of size, make them superior to organic dyes for biological applications: microscopy and multiplexed histology, flow cytometry, drug delivery, photodynamic therapy, clinical imaging, real time detection of intracellular events etc. Thus, QDs and their alliance with other aspects of nanotechnology have opened new possibilities for advanced molecular and cellular imaging as well as for ultrasensitive bioassays, diagnostics and therapeutics. However, high-quality and well-controlled trials specifically addressing certain inherent pitfalls of QDs will need to be undertaken before these can be considered for human use.
KEYWORDS: Quantum dots; nanotechnology; medicine; diagnosis; therapy.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Mishr RK ,Dhillon J, Pandey R, Sodhi KS,Singh J. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Review article
Kanda, Raquel, G*.,(Junior Fellow), França, Bianca, M., (Junior Fellow),Cerino, Elizabelle, G.,(Junior Fellow),Baima, Eduardo, F.,(Junior Fellow), Brito, Israel, P., (Junior Fellow), Texeira, Antonio, G.J.,(Junior Fellow), Cartaxo, Jesus, S., Dr. Souza, Sonia, I. R., Dr.Peixoto, Flórido, S. N., Cardoso, Maria Auxiliadora, B. S., Dr.Santos, Maria do Socorro, V., Silva, Cláudio Gleidiston, L., Dr. Kanda, Paulo M. (PhD), Quirino, João de Deus, Rolim-Neto, Modesto, L.,(PhD)
Affiliation:-
*Federal University of Cariri, Divine Savior Street, 284, Center, Barbalha, Ceara, Brazil
Institutional Program for Scientific Initiation (PIBIC) / National Council for Scientific and Technological Development (CNPq) fellow at Faculty of Medicine, Federal University of Cariri, Barbalha, Ceara, Brazil.
Postgraduate Program in Health Sciences - Faculty of Medicine of ABC, Santo André, São Paulo, Brazil
Neurovale. The electroencephalograph(EEG) laboratory. Taubaté. São Paulo, Brazil
The name of the department(s) and institution(s) to which the work should be attributed:
1.Federal University of Cariri, Divine Savior Street, 284, Center, Barbalha, Ceara, Brazil.
2.Institutional Program for Scientific Initiation (PIBIC) / National Council for Scientific and Technological Development (CNPq) fellow at Faculty of Medicine, 3.Federal University of Cariri, Barbalha, Ceara, Brazil.
Postgraduate Program in Health Sciences - Faculty of Medicine of ABC, Santo André, São Paulo, Brazil
4.Neurovale. The electroencephalograph(EEG) laboratory. Taubaté. São Paulo, Brazil
Address reprint requests to
Kanda, Raquel, G.
(Labesci- Federal University of Cariri) Divine Savior Street, 284, Center, Barbalha, Ceará, Brazil. Zip Code: 63180000
Article citation:
Kanda RG,França BM,Cerino EG,Baima EF,Brito, IP,Texeira, A GJ et al. Road map To schizophrenia genetics: A qualitative systematic review. J Pharm Biomed Sci. 2014; 04(12):1132-1157 Available at www.jpbms.info
ABSTRACT
Background: The studies that focus on the genetic basis of psychopathologies become increasingly frequent and important. Consequently, genetic markers may be the new instrument to assist the diagnostic accuracy of some complex diseases such as Schizophrenia. Aims: To identify the genes those are involved in the schizophrenia’s etiology and their distribution around the world. Methods: we performed a systematic literature research of studies from January 2004 to august 2014 held by SCOPUS and MEDLINE. The descriptors used in both databases were “genetic markers (medical subject headings [MeSH term])”, “mutation (MeSH term)” and “schizophrenia (MeSH term)”. Of the 149 articles found, 39 met the eligibility criteria. RESULTS: Many genetic markers are hypothesized to be linked to Schizophrenia. Genetic studies agree with the clinical observation that schizophrenia is highly heritable but the genetics are complex. Although, some genes such as CAPON, COMT, HUMAN QKI, NR2E1, ProDH and ADRBK2 appears to be frequent in more than one nationality. Conclusion: Therefore, these markers must be studied with larger sample sizes to confirm these promising findings. This study also presents a geographical distribution of the genetic markers around the world with its implications to schizophrenia. Interesting results were observed in the genetic patterns of different ethnicities. However, there remains some doubt about the genes identified and further investigation and wider confirmation are needed as both positive and negative replication studies have been reported in different countries, and it is likely that some initial studies have produced false positives.
KEYWORDS: Genetics; Schizophrenia; Systematic Review.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Copyright © 2014. Kanda RG, França BM, Cerino EG, Baima EF, Brito, IP, Texeira, A GJ, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Short Communication
Kanda, Raquel, G*.,(Junior Fellow),França, Bianca, M.,(Junior Fellow),Cerino, Elizabelle, G.,(Junior Fellow),Baima, Eduardo, F.,(Junior Fellow), Lima,Jobson, N.F.,(Junior Fellow),Feitosa,Uilna, N. S,Dr.Lima, Nádia Nara, R.,Dr.Damasceno, Kécia, S.,Dr. Kanda, Paulo, M.,(PhD), Rolim-Neto, Modesto, L.(PhD).
Affiliation:-
*Federal University of Cariri, Divine Savior Street, 284, Center, Barbalha, Ceara, Brazil
Institutional Program for Scientific Initiation (PIBIC) / National Council for Scientific and Technological Development (CNPq) fellow at Faculty of Medicine, Federal University of Cariri, Barbalha, Ceara, Brazil.
Postgraduate Program in Health Sciences - Faculty of Medicine of ABC, Santo André, São Paulo, Brazil
Neurovale. The electroencephalograph (EEG) laboratory. Taubaté. São Paulo, Brazil
The name of the department(s) and institution(s) to which the work should be attributed:
1.Federal University of Cariri, Divine Savior Street, 284, Center, Barbalha, Ceara, Brazil.
2.Institutional Program for Scientific Initiation (PIBIC) / National Council for Scientific and Technological Development (CNPq) fellow at Faculty of Medicine, 3.Federal University of Cariri, Barbalha, Ceara, Brazil.
Postgraduate Program in Health Sciences - Faculty of Medicine of ABC, Santo André, São Paulo, Brazil
4.Neurovale. The electroencephalograph (EEG) laboratory. Taubaté. São Paulo, Brazil
Address reprint requests to
Kanda, Raquel, G.
(Labesci- Federal University of Cariri) Divine Savior Street, 284, Center, Barbalha, Ceará, Brazil. Zip Code: 63180000
Article citation:
Kanda RG,França BM,Cerino EG,Lima J NF,Feitosa U NS,Lima NN R,et al. Sexual Harassment: University Stigmata. J Pharm Biomed Sci. 2014; 04(12):1128-1131. Available at www.jpbms.info
ABSTRACT
The recurrence of rapes, stalking, jokes and other types of opression against women in renowned universities raises a question: how is it possible to occur so many cases of sexual violence in this setting, where freedom of expression, criticality and equality are paramount values?
Our research revealed many scenarios of sexual abuse, such as Columbia University, State University of São Paulo (USP) and Federal University of Minas Gerais (UFMG), besides other institutions in China, Mozambique and United Kingdom. In most cases the university, that has a lack of legislation about sexual violence, underestimated the act severity and the aggressor did not undergo major punishments. It was also largely reported alcohol association with harassment, harasser's threat and victim's fear to deal with the situation.
We concluded that this phenomenon has a multifactorial cause, including victim’s ignorance about their rights and social hedonist perception of life. The criticality, dignity and equality must be rescued in the academic setting by the University, that has the obligation to provide a safe environment for women to become politically active and knowledgeable of their rights.
KEYWORDS: Abuse; College; Harassment; Sexual; University.
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Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Copyright © 2014. Kanda RG,França BM,Cerino EG,Lima J NF,Feitosa U NS,Lima NN R,et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.