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Original article
Abdullah Edreis Abdullah1,*, Adel Nasr Morsi2 Mahgoub Mohammed Elhassan Faragalla3, Mohammed MerghaniElsayed4
Affiliation:-
1Department of Chemical Pathology, Faculty of Medical Laboratory Sciences, National University-Sudan /Department of medical laboratories.
Yagoub Medical Center, Khartoum, Sudan.
2Department OF Chemical Pathology, Faculty of Medical Laboratory Sciences, University of Khartoum-Sudan.
3Department of Clinical Laboratory, The International Center for Medical Investigations, Khartoum, Sudan
4Department of chemical pathology Ribat National Hospital, Ministry of Interior Khartoum, Sudan.
The name of the department(s) and institution(s) to which the work should be attributed:
University of Khartoum, Faculty of Medical Laboratory Sciences, Department of chemical pathology, Khartoum, Sudan
Address reprint requests to
Abdullah Edreis Abdullah Ehmedi.
Department OF Chemical Pathology, Faculty of Medical Laboratory Sciences, National University-Sudan.
Yagoub Medical Center, Khartoum, Sudan
Tell: +249913501900
Fax:+249 155775788
Article citation:
Abdullah AE, Morsi AN, Elhassan Faragalla MM, Elsayed MM. The association between male infertility and diabetes mellitus. J Pharm Biomed Sci.2014; 04(12):1097-1102. Available at www.jpbms.info
ABSTRACT:
Background: Infertility is already a major health problem in both the developed and developing world, with up to one in six couples requiring specialist investigation or treatments in order to conceive. DM has a significant impact on the fertility of men with this disease both directly and indirectly. There are a number of reports in the literature examining the effects of diabetes on the sperm parameters.
Methodology: This is a retrospective case control study based on data collected by andrology and fertility clinics. The laboratory results of semen analysis (semen volume, sperm count, motility and morphology) was collected for 100 diabetic (mean age 37.4 years) and 100 nondiabetic subjects (control group, men undergoing routine infertility investigations, mean age 37.2 years).The data was analyzed using SPSS program and p value was calculated. Results: The patient’s semen parameters differ significantly from control subjects. A reduction in all semen parameters (semen volume, sperm count, motility and morphology) has been observed in the diabetic patient group. Diabetic subjects had significantly lower mean of semen volume, sperm count, motility and morphology compared with control subjects.
Conclusion: The present study findings revealed that there is an association between infertility and Diabetes Mellitus in Sudanese men.
KEYWORDS: Diabetes Mellitus; Male infertility; Sudan.
REFERENCES
1.ADA. Report of the Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Diabetes Care 1997;20:1183–97. 2. Castano L, Eisenbarth GS. Type-I diabetes: a chronic autoimmune disease of human, mouse, and rat. Annu Rev Immunol 1990;8:647–79.
2.Williams textbook of endocrinology (12thed.). Philadelphia: Elsevier/Saunders. Pp. 1371–1435. ISBN978-1-4377-0324-5.
3.Vos, T; Flaxman, AD et al (2012 Dec 15). “Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010.”. Lancet 380 (9859): 2163–96. PMID23245607
4.Emerging Risk Factors Collaboration (2010). “Diabetes mellitus, fasting blood glucose concentration, and risk of vascular disease: A collaborative meta-analysis of 102 prospective studies”. The Lancet 375 (9733): 2215–22. doi:10.1016/S0140-6736(10)60484-9. PMC2904878. PMID20609967.
5.Sexton WJ, Jarow JP. Effect of diabetes mellitus upon male reproductive function. Urology 1997;49:508–513.
6.Padro´n RS, Dambay A, Sua´ rez R, Ma´ s J. Semen analyses in adolescent diabetic patients. ActaDiabetol Lat.1984;21:115–121.
7.Ali ST, Shaikh RN, Siddiqi NA, Siddiqi PQ. Semen analysis in insulindependent/ non-insulin-dependent diabetic men with/without neuropathy. Arch Androl. 1993;30:47–54.
8.Barta´k V, Josı´fko M, Hora´ckova´ M. Juvenile diabetes and human sperm quality. Int J Fertil. 1975;20:30–32.
9.Padro´n RS, Dambay A, Sua´ rez R, Ma´ s J. Semen analyses in adolescent diabetic patients. ActaDiabetol Lat.1984;21:115–121.
10.Agbaje IM, Rogers DA, McVicar CM, McClure N, Atkinson AB, Mallidis C, Lewis SE. Insulin dependent diabetes mellitus: implications for male reproductive function. Hum Reprod. 2007;22:1871–1877.
11.Delfino M, Imbrogno N, Elia J, Capogreco F, Mazzilli F. Prevalence of diabetes mellitus in male partners of infertile couples. Minerva UrolNefrol. 2007;59:131–135.
12.Frenkel GP, Homonnai ZT, Drasnin N, et al. Fertility of the streptozotocin-diabetic male rat. Andrologia (1978) 10:127–136.
13.Albert KG, Zimmet PZ (1998) Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 15:539–553. doi:10.1002/(SICI)1096-9136(199807)15:7\ 539::AID-DIA668[3.0.CO;2-S.
14.Kim SH, Chunawala L, Linde R, Reaven GM (2006) Comparison of the 1997 and 2003 American Diabetes Association classification of impaired fasting glucose: impact on prevalence of impaired fasting glucose, coronary heart disease risk factors, and coronary heart disease in a community-based medical practice. J Am CollCardiol 48:293–297. doi:10.1016/j.jacc.2006.03.043.
15.WHO (1991) Infertility: a tabulation of available data on prevalence of primary and secondary infertility, Geneva:WHO program on maternal and child health and family planning, Division of Family Health.
16.Hull MG, Glazener CM, Kelly NJ et al. Population study of causes, treatment, and outcome of infertility. Brit Med J (Clin Res Ed) 1985;291:1693–1697.
17.Thonneau P, Marchand S, Tallec A et al. Incidence and main causes of infertility in a resident population (1,850,000) of three French regions (1988–1989). Hum Reprod 1991;6:811–816.
18.Carlsen E, Giwercman A, Keiding N et al. Evidence for decreasing quality of semen during past 50 years. Brit Med J 1992;305:609 613.
19.Bonde JP, Ernst E, Jensen TK et al. Relation between semen quality and fertility: a population-based study of 430 first-pregnancy planners. Lancet 1998;352:1172–1177.0
Source of support: None.
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2014 Abdullah AE, Morsi AN, Elhassan Faragalla MM, Elsayed MM. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Mohamed Marzuk S,Prabhu N.*,Radhakrishna L,Sarada V
Affiliation:-
Department of Microbiology, Chennai Medical College Hospital and Research Centre (SRM Group), Tiruchirapalli – 621 105, Tamilnadu, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, Chennai Medical College Hospital and Research Centre (SRM Group), Tiruchirapalli – 621 105, Tamilnadu, India
Address reprint requests to
Prabhu N, Ph.D.
Department of Microbiology, Chennai Medical College Hospital and Research Centre (SRM Group), Tiruchirapalli – 621 105, Tamilnadu, India
Article citation:
Mohamed Marzuk S, Prabhu N, Radhakrishna L, Sarada V. Urine examination for determining the types of crystals – a comparative approach related to pH. J Pharm Biomed Sci. 2014; 04(12):1072-1078. Available at www.jpbms.info
ABSTRACT
Background: Studies on the frequency of the different types of urinary crystals and the role of microscopic examination for identification is still uncertain. The analytical complexity of urine particles is largely responsible to understand the pathophysiology of crystal formation. As a result, there is no consensus regarding technical methods in crystalluria with several techniques for urine sampling and three different instruments currently used for particle study, namely particle counting (PC), light microscopy (LM) and petrographic microscopy (PM). Aim: In this work, we first examined urine samples suspected for crystalluria and compared with pH analysis. Settings and Design: We describe the results on the prevalence and typology of crystalluria of the urine samples received in the clinical laboratory of tertiary care rural teaching hospital. Material and Methods: The methodology including the identification of the combined knowledge of crystal morphology with its acidity and alkalinity nature; further confirmation required with infrared spectroscopy and microbial culture analysis. Results and Conclusion: The results highlighted the presence of different types of crystals in the urine samples and strongly supported the pH ranges. The variations in the pH range from 4 to 8. The ratio of 7.5:3.5 was identified among normal and abnormal crystals respectively. Among the normal crystals, calcium oxalate was found in 28% of urine samples and 29% of cystine found among abnormal crystals. The correlation of the results of pH with the crystal formation was well studied and further it gets proved with the theory and reference interpretation.
KEYWORDS: Crystalluria; Calcium oxalate; Cystine; pH ranges; Prevalence.
REFERENCES
1.Daudon M, Hennequin C, Boujelben G, Lacour B, Jungers P. Serial crystalluria determination and the risk of recurrence in calcium stone formers. Kid Intern. 2005; 67: 1934-1943.
2.Taller A, Grone B, Rogers KA, Goldberg HA, Hunter GK. Specific adsorption of Osteopontin and synthetic polypeptides to calcium oxalate monohydrate crystals. Biophys J. 2007; 93: 1768-1777.
3.Verdesca S, Fogazzi GB, Garigali G, Messa P, Daudon M. Crystalluria: prevalence, different types of crystals and the role of infrared spectroscopy. Clin Chem Lab Med. 2011; 49: 515-520.
4.Murayama T, Taguchi H. The role of the diurnal variation of urinary pH in determining stone compositions. J Urol. 1993; 150: 1437-1439.
5.Murayama T, Sakai N, Yamada T, Takano T. Role of the diurnal variation of urinary pH and urinary calcium in urolithiasis: a study in outpatients. Int J Urol. 2001; 8: 525-531.
6.Osborne CA, Polzin DJ, Kruger JM, Lulich JP, Johnston GR, O’Brian TD. Relationship of nutritional factors to the cause, dissolution and prevention of feline uroliths and urethral plugs. Vet Clin North Am. 1989; 19: 561-581.
7.Hesse A. Canine urolithiasis: epidemiology and analysis of urinary calculi. J. Small Anim Pract. 1990; 31: 599-604.
8.Ling GV, Franti CE, Ruby AL, Johnson DL. Urolithiasis in dogs II: breed prevalence and interrelationships of breed, sex, age, and mineral composition. Am J Vet Res. 1998; 59: 630-642.
9.Jeffrey BK, Kirk DM, Jo AMM, Irwin MF, Ellen VRN, Chandra BBS, Lewis KP, Judith F. Crystalluria and urinary tract abnormalities associated with Indinavir. Ann Intern Med. 1997;127:119-125.
10.Daudon M, Bounxouei B, Santa Cruz F, Leite da Silva S, Diouf B, Angwafoo FF, Talati J, Desrez G. Composition of renal stones currently observed in non-industrialized countries. Prog Urol. 2004;14: 1151-1161.
11.Meissner A, Mamoulakis C, Laube N. Urinary tract infections and urolithiasis. Urologe A. 2010; 49: 623-628.
12.Goldfarb DS. Microorganisms and calcium oxalate stone disease. Nephron Physiol. 2004;98:48-54.
13.Bichler KH, Eipper E, Naber K, Braun V, Zimmermann R, Lahme S. Urinary infection stones. Int J Antimicrob Agents. 2002;19:488-498.
14.Mahmood Z, Zafar SA. Review of pediatric patients with urolithiasis, in view of development of urinary tract infection. J Pak Med Assoc. 2008;58: 653-656.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript
Copyright © 2014 Mohamed Marzuk S ,Prabhu N, Radhakrishna L, Sarada V. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.