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Original article
Bouchra Rahmouni1,*, Katim Alaoui2, El Houcine Bouidida1,2,3, Malika chammache4, Mohammed Sghir Taleb5, Yahia Cherrah2, Abdelkader Il Idrissi1
Affiliation:
1Department of Chemistry, Laboratory of Plants Chemistry, Organic and Bio- Organic Synthesis, Faculty of Sciences, University MohammedV, Agdal, Rabat, BP 1014, Morocco
2Pharmacology and Toxicology Laboratory, Faculty of Medicine and Pharmacy, Rabat, Morocco
3National Drug Control Laboratory, Direction of Drug and Pharmacy, Ministry of Health, BP 6203, Rabat-Institutes, Rabat, Morocco
4Department of Chemistry, heterocyclic chemistry Laboratory, Faculty of Sciences, University MohammedV, Agdal, Rabat, Morocco, Rabat, BP 1014, Morocco
Department of Botany and Plant Ecology, Scientific Institute, University Mohammed V, Rabat, Morocco
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Chemistry, Laboratory of Plants chemistry, Organic and Bio- Organic Synthesis, Faculty of Sciences, University MohammedV, Agdal, Rabat, BP 1014, Morocco
Address reprint requests to
* Bouchra Rahmouni
Department of Chemistry, Laboratory of Plants chemistry, Organic and Bio- Organic Synthesis, Faculty of Sciences,University MohammedV, Agdal, Rabat, BP 1014, Morocco
Tel : (+212) 6 63238191
Fax:(+212) 5 37 68 19 31
Article citation:
Rahmouni B,Alaoui K,Bouidida El-H,Chammache M,Taleb MS,Cherrah Y,Idrissi A. Acute toxicity, analgesic activity of Nepetagranatensis essential oil. J Pharm Biomed Sci. 2015; 05(02):110-117. Available at www.jpbms.info
ABSTRACT:
To evaluate the acute toxicity and the peripheral and central analgesic activity of the genus Nepeta, we studied the essential oil of Nepetagranatensis Boiss spontaneous species of Morocco. We have obtained the essential oil of N. granatensis by microwave irradiation and identified by GC-MS.
Female mice IOPS ofa,20-30g,were used to study the acute toxicity, as well as for the peripheral analgesic activity (Koster test). However, the study of the central analgesic activity (tail flick test) was conducted on rats Waster 250-350g. Doses of 25mg/kg and 50mg/kg of EO were administered intraperitoneally for both peripheral and central analgesic activities.The identification of the EO led to 25 constituent whose nepetalactone is the major product.The essential oil revealed a LD50 of 1000mg/kg. Koster test has shown a major protective power against abdominal cramps 74, 11 % at 25mg/kg and reached 86.77 % at 50mg/kg. However, the reflex time in the tail flick test exceeded the threshold of pain inhibition, which reached 8.46 s for the 50mg/kg dose.
EO had proved a low acute toxicity, and a great potential analgesic activity type ASA and morphine- like at 50mg/kg, which is probably due to its richness in nepetalactone
KEYWORDS: Acute toxicity; Analgesic activity; Essential oil; Microwave; Nepetagranatensis; nepetalactone.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015. Rahmouni B, Alaoui K, Bouidida El-H, Chammache M, Taleb MS, Cherrah Y, IL Idrissi A. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Kirti Jaiswal1,*, Sandip Kumar2, Santosh Kumar Sant3, Amit Kant Singh4,
ANG Hyder5, Abid Ahsan6
Affiliation:
1Associate Professor, Department of Physiology, UP RIMS & R, Saifai, Etawah, U.P, India
2AssociateProfessor, Department of Community Medicine, UP RIMS & R, Saifai, Etawah, India
3Associate Professor, Department of Physiology, UP RIMS & R, Saifai, Etawah, U.P, India
4Associate Professor, Department of Physiology, UP RIMS & R, Saifai, Etawah, U.P, India
5Lecturer, Department of Physiology, UP RIMS & R, Saifai, Etawah, U.P, India
6Assistant Professor, Department of Physiology, Hind Institute of Medical Sciences, Barabanki UP, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Physiology, UP RIMS & R, Saifai, Etawah, U.P, India
Department of Community Medicine, UP RIMS & R, Saifai, Etawah, India
Hind Institute of Medical Sciences, Barabanki UP, India
Authors contribution:
Author 1 &3 design the study, 5 & 6 collected the data and 2 & 4 analyse the data.
Address reprint requests to
Dr. Kirti Jaiswal
Associate Professor,
Department of Physiology,
UP Rural Institute of Medical Sciences & Research,
Saifai, Etawah, 206 130 (U.P.), India or at drkirtijaiswal@yahoo.com
Article citation: Jaiswal K,Kumar S,Sant KS,Singh AK,Hyder ANG,Ahsan A. A Study on Pulmonary Function Tests in Bangle workers of a district of central Uttar Pradesh, India J Pharm Biomed Sci.2015;05(02):90-94.Available at www.jpbms.info
ABSTRACT:
The pulmonary function impairment is a common respiratory problem among industrial exposure. Therefore, the present study was undertaken to evaluate the effects of furnace smoke and flue and its duration of exposure on lung function. This is a matched cross-sectional study of Spirometry in 100 bangle workers with age range 20 – 60 years, who worked without smoke control ventilation or respiratory protective devices. Pulmonary function tests were performed by using Digital Spirometer (Spiro-excel). Significant, reduction was observed in the mean values of Forced Vital Capacity (FVC), Forced Expiratory Volume in one second (FEV1), Forced Expiratory Ratio (FEV1/FVC%), Forced Expiratory Flow (25% -75%) and Peak Expiratory Flow Rate (PEFR) in bangle workers relative to their matched controls. It is concluded that lung functions in bangle workers is impaired.
KEYWORDS: Occupational hazards; pulmonary function; bangle workers.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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Source of funding: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015. Jaiswal K, Kumar S, Sant KS, Singh AK, Hyder ANG, Ahsan A. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.