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Original article
Hitesh R Ahir1,* Parimal H Patel1,†, Alka B Nerurkar1,¥
Affiliation:
1,*Tutor, 1,†Assistant Professor, 1,¥Professor and Head of Department, Department of Microbiology, GMERS, Medical College, Valsad, Gujarat, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, GMERS, Medical College, Valsad, Gujarat, India
Address reprint requests to
Hitesh R Ahir*
Department of Microbiology, GMERS, Medical College, Valsad, Gujarat, India
Article citation: Ahir HR, Patel PH, Nerurkar AB. Intestinal Parasitic Infections in Patients attending Tertiary Care Hospital, Valsad, south Gujarat, India: A retrospective study. J Pharm Biomed Sci. 2015; 05(02):117-121. Available at www.jpbms.info
ABSTRACT:
Intestinal parasitic infections are widely prevalent in developing countries due to poor sanitation and inadequate personal hygiene. They are responsible for considerable morbidity and mortality.
Aim: The study was undertaken to assess the prevalence of Intestinal Parasitic Pathogens amongst patients attending a tertiary care hospital in district valsad, Gujarat.
Materials and Methods: Stool samples from 291 patients which included 120 females and 171 males were collected and screened using conventional saline and iodine wet mount and examined by direct microscopy.
Results: In our study the prevalence of intestinal parasitic infection was found to be 13.40%. E.histolytica (45.23%) and A. duodenale ( 26.19%) were the commonest parasites isolated, followed by A lumbricoides (11.90%), S stercoralis (11.90%) and Hymenolepis nana (4.76%). Infection rate was highest in females (15 %) than males (12.28%).The infestation was higher in the 21-30 years age group as compared to others. Multiple parasitic infection are detected in three stool samples(7%).
Conclusion: Helminthic infections are more common than protozoal. The present study reports that intestinal parasitic infection is prevalent in this area and this study also indicate the usefulness and easy to perform stool examination at very low cost.
KEYWORDS: Intestinal parasitic infections.
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7.Baragundi MC, Sonth SB, Solahannwar S, Patil CS. The prevalence of parasitic infections in patients attending teritiary care hospital. Nat J Bas Med Sci 2011; 2(1):31-4.
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12.M.S. Davane1, N.M. Suryawanshi, K.D. Deshpande.A Prevalence Study of Intestinal Parasitic Infections in a Rural HospitalInternational Journal of Recent Trends in Science And Technology. 2012;2(1): 01-03.
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15. Shrihari Narayan, Kumudini T.S, Mariraj. JD, Krishna. S. The Prevalence of Intestinal Parasitic Infections in a Tertiary Care Hospital-a retrospective study.J Pharm Biomed Sci.(JPBMS).2011;12:(08). Accessed from www.Jpbms.info.
16.Vaishali R Wabale, Dnyaneshwari P Ghadge, Abhay S Chowdhary, Renu S Bharadwaj . Parasitic Infestation in and around Mumbai. BMJ. 2006; 333 : 779.
17.WHO, Guidelines for evaluation of Soil transmitted helminthiasis and schistosomiasis at community level, schistosomiasis and intestinal parasite unit, WHO, Geneva, WHO/CTD/SIP/98.1, 1998.
18.Baldock FC, Arthur RJ. A survey of fascioliasis in beef cattle killed at abattoirs in southern Queensland.Aust Vet J. 1985;62:324-6.
19.Remoue F. Functional specific binding of testosterone to Schistosoma haematobium 28-kilodalton glutathione S-transferase. Infect Immun 2002;70:601-5.
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22.S Satheesh kumar, S Ananthan, P Lakshmi Intestinal parasitic infectionin HIV infected patients with diarrhoea in chennai year. 2002;20(2):Page :88-91.
23.Baragundi MC, Sonth SB, Solahannwar S, Patil CS. The prevalence of parasitic infection in patients attending tertiary care hospital. Nat J Bas Med Sci. 2011; 2(1):31-4.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Ahir HR, Patel PH, Nerurkar AB. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Guo Chen1, Zhenghua Ni2, Qingge Chen1, Xiongbiao Wang1,*
Affiliation:
1Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, PR China
2Central lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, PR China
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine &
2. Central lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China
Address reprint requests to
Xiongbiao Wang.
Central lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062,PR China
Article citation:
Wang et al. Gene silencing of IL-13 by siRNA aerosol inhalation inhibits asthma phenotype in the murine model of asthma. J Pharm Biomed Sci. 2015; 05(02):126-130. Available at www.jpbms.info
ABSTRACT:
Background: interleukin-13 has been shown to be a critical mediator of the asthma phenotype. It was reported that intravenously administration of the IL-13 siRNA in vivo reduced airway resistance significantly in the murine asthma model.
Objectives: This study was intended to investigate whether aerosol inhalation of IL-13 siRNA was effective in reduce IL-13 expression and thus attenuated asthma phenotype.
Methods: The ovalbumin (OVA)-challenged mouse model of asthma was used to assess the effect of IL-13 interference. Airway inflammation in lung tissue was assessed using hematoxylin and eosin (H&E). The mRNA expression of IL-13 gene was determined using real-time PCR.
Results: Aerosol inhalation of the IL-13 siRNA was shown to reduce the expression of IL-13 and reduced airway inflammation in lung tissues compared with control group.
Conclusion: Our results demonstrated the effectiveness of aerosol inhalation of IL-13 siRNA in preventing airway inflammation in vivo and suggested potential therapeutic applications.
KEYWORDS: IL-13; siRNA; asthma; aerosol inhalation.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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1.Wills-Karp M (2004) Interleukin-13 in asthma pathogenesis. Immunol Rev 202: 175-190.
2.Hansbro PM, Scott GV, Essilfie AT, Kim RY, Starkey MR, et al. (2013) Th2 cytokine antagonists: potential treatments for severe asthma. Expert Opin Investig Drugs 22: 49-69.
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Source of funding: This work was supported by the Shanghai Science and Technology Committee (No.10411969100, No.12401900404).
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015. Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
P.Vijayalakshmi* M.D (Microbiology)
Affiliation:
Department of Microbiology, Malla Reddy Medical College for Women, Suraram, Hyderabad, Telangana, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, Malla Reddy Medical College for Women, Suraram, Hyderabad, Telangana, India
Address reprint requests to
*Dr.P.Vijayalakshmi.
Professor & H.O.D
Department of Microbiology
Malla Reddy Medical College for Women
Suraram, Hyderabad, Telangana, India
Article citation: Vijayalakshmi P. Association of STD with HIV. J Pharm Biomed Sci. 2015; 05(02):166-167. Available at www.jpbms.info
ABSTRACT: Background: STDs such as genital ulcer disease and possibly chlamydia trachomatis genital infections, facilitate the sexual transmission of HIV infection, thus making STD clinic attenders potentially more susceptible to HIV transmission.
Objective:To determine the prevalence of HIV in STD patients.
Methods: Data was collected from 200 patients suffering from STDs for a period of one year.
Blood samples were collected from 100 normal, healthy, voluntary blood donors to serve as a control for the present study. The diagnosis of the various STDs was established by standard laboratory investigations done with blood, urine, cervical, vaginal and urethral swabs and exudates from the lesions.
The VDRL test was routinely performed on all the sera collected.
ELISA test was employed for HIV screening. The ELISA test was repeated in positive cases and confirmation was done by the Western Blot test, at the National Institute of Virology, Pune.
Results: Of the 20 reactive cases, 17 were found to be suffering from genital ulcerative disease, predominantly syphilis and chancroid .
Conclusion: HIV infection was found to be more in younger age groups, more in males than in females.
KEYWORDS: HIV; STD; Prevalence.
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Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Vijayalakshmi P. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Bouchra Rahmouni1,*, Katim Alaoui2, El Houcine Bouidida1,2,3, Malika chammache4, Mohammed Sghir Taleb5, Yahia Cherrah2, Abdelkader Il Idrissi1
Affiliation:
1Department of Chemistry, Laboratory of Plants Chemistry, Organic and Bio- Organic Synthesis, Faculty of Sciences, University MohammedV, Agdal, Rabat, BP 1014, Morocco
2Pharmacology and Toxicology Laboratory, Faculty of Medicine and Pharmacy, Rabat, Morocco
3National Drug Control Laboratory, Direction of Drug and Pharmacy, Ministry of Health, BP 6203, Rabat-Institutes, Rabat, Morocco
4Department of Chemistry, heterocyclic chemistry Laboratory, Faculty of Sciences, University MohammedV, Agdal, Rabat, Morocco, Rabat, BP 1014, Morocco
Department of Botany and Plant Ecology, Scientific Institute, University Mohammed V, Rabat, Morocco
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Chemistry, Laboratory of Plants chemistry, Organic and Bio- Organic Synthesis, Faculty of Sciences, University MohammedV, Agdal, Rabat, BP 1014, Morocco
Address reprint requests to
* Bouchra Rahmouni
Department of Chemistry, Laboratory of Plants chemistry, Organic and Bio- Organic Synthesis, Faculty of Sciences,University MohammedV, Agdal, Rabat, BP 1014, Morocco
Tel : (+212) 6 63238191
Fax:(+212) 5 37 68 19 31
Article citation:
Rahmouni B,Alaoui K,Bouidida El-H,Chammache M,Taleb MS,Cherrah Y,Idrissi A. Acute toxicity, analgesic activity of Nepetagranatensis essential oil. J Pharm Biomed Sci. 2015; 05(02):110-117. Available at www.jpbms.info
ABSTRACT:
To evaluate the acute toxicity and the peripheral and central analgesic activity of the genus Nepeta, we studied the essential oil of Nepetagranatensis Boiss spontaneous species of Morocco. We have obtained the essential oil of N. granatensis by microwave irradiation and identified by GC-MS.
Female mice IOPS ofa,20-30g,were used to study the acute toxicity, as well as for the peripheral analgesic activity (Koster test). However, the study of the central analgesic activity (tail flick test) was conducted on rats Waster 250-350g. Doses of 25mg/kg and 50mg/kg of EO were administered intraperitoneally for both peripheral and central analgesic activities.The identification of the EO led to 25 constituent whose nepetalactone is the major product.The essential oil revealed a LD50 of 1000mg/kg. Koster test has shown a major protective power against abdominal cramps 74, 11 % at 25mg/kg and reached 86.77 % at 50mg/kg. However, the reflex time in the tail flick test exceeded the threshold of pain inhibition, which reached 8.46 s for the 50mg/kg dose.
EO had proved a low acute toxicity, and a great potential analgesic activity type ASA and morphine- like at 50mg/kg, which is probably due to its richness in nepetalactone
KEYWORDS: Acute toxicity; Analgesic activity; Essential oil; Microwave; Nepetagranatensis; nepetalactone.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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10.Srifi A, Rahmouni B, Bouidida El H, Alaoui K, Y Cherrah Y, Il Idrissi A, et al.Etude phytochimique et activité antifongique in vitro des huiles essentielles de quatre espèces du genre Nepeta du Maroc. Phytothérapie.2013; 11: 161-71.
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Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015. Rahmouni B, Alaoui K, Bouidida El-H, Chammache M, Taleb MS, Cherrah Y, IL Idrissi A. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Vaishali D. Kotasthane1,*, Dhananjay S Kotasthane1, G Koteeswaran1,Alok Mohanty2
Affiliation:
1Department of Pathology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Puducherry -607402, India
2Department of General Surgery, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Puducherry -607402, India
The name of the department(s) and institution(s) to which the work should be attributed:
Mahatma Gandhi Medical College and Research Institute,SBV University, Pillaiyarkuppam, Puducherry -607402, India
Address reprint requests to
Dr Vaishali D. Kotasthane, MD(Pathology),
Assistant professor, Department of Pathology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Puducherry-607402. India
Article citation: Kotasthane VD, Kotasthane DS, Koteeswaran G, Mohanty A. Clinicopathological study of colorectal tumors: A four year study in a rural tertiary care hospital in South India. J Pharm Biomed Sci. 2015; 05(02):139-146. Available at www.jpbms.info
ABSTRACT: Background: Colorectal tumors are thought to be comparatively uncommon in developing countries like India. Recent studies showed steady increase in incidence of colorectal cancers for developing countries. Aims: The aim of the present study was to evaluate histological types of colorectal tumors with respect to demographic data like age, gender distribution, anatomical site and grading.
Material and methods: This was a descriptive study of colorectal biopsies/specimens from May 2008 to May 2012 carried out in Department of Pathology, Mahatma Gandhi Medical College and Research Institute, a tertiary care hospital in Pondicherry. Relevant information like age, sex, site and clinical features was noted.
Statistical method: The data compiled was analyzed for demographic and biographical details like age, sex, site, clinical features, gross findings and light microscopic histopathological diagnosis. Descriptive analysis were reported as mean and standard deviation of continuous variables. Unpaired t test and chi-square test were used and value of p<0.05 was taken as statistically significant.
Results and conclusions: Total 68 colorectal tumors were observed, representing 6.7% of all tumors during study period. Out of these, 60.3% were malignant. Rectal carcinomas predominated over colonic carcinomas. For Colorectal carcinomas (CRCs), mean age at presentation was 61.8 years in males and 50.8 years in females which was significantly lower (p=0.0012) with Adenocarcinoma-NOS as commonest subtype. Early age at presentation in females, suggests that colonoscopic screening will be useful for early detection. Quantitative estimates of this study are of interest for future population-based studies and are potentially useful for targeting screening colonoscopy programmes.
KEYWORDS: Colorectal tumors; Adenocarcinomas; Polyps;Adenomas.
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Copyright © 2015 Kotasthane VD, Kotasthane DS, Koteeswaran G, Mohanty A. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.