DocumentsDate added
Original research article
Quebral, Elgin Paul B.† and Medina, Paul Mark B.*
Affiliation:
†MD-PhD in Molecular Medicine Program, College of Medicine, University of the Philippines Manila, Ermita, Manila, Philippines
*Faculty, Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Ermita, Manila, Philippines
Author contributions: Both authors contributed equally to this paper.
Address reprint requests to:
Medina, Paul Mark B.
Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Pedro Gil Street, Ermita, 1000 Manila, Philippines
Office Telephone/Fax: +632 526 0377; +632 526 4197
Article citation:
Quebral, EPB & Medina, PMB. Crude anthocyanin extract (CAE) from Ballatinao black rice hastens regeneration in Dugesia hymanae. J Pharm Biomed Sci. 2015; 05(02):147-153. Available at www.jpbms.info
ABSTRACT: Anthocyanins are a major group of flavonoids that have been examined for various medical and pharmacological applications. However, very few studies have explored the potential of anthocyanins for wound healing and regeneration. We investigated if a crude anthocyanin extract (CAE) from Ballatinao black rice (Oryza sativa L. tropical japonica) can affect regeneration in planarians (Dugesia hymanae). The planarians were transversely cut into two equal parts and were exposed to different concentrations of CAE. Our findings showed that 0.5 μg/ml and 5.0 μg/ml of CAE significantly shortened regeneration time of missing anterior portions of cut planarians by 7.7% (p<0.05). Additionally, 0.5 μg/ml and 5.0 μg/ml of CAE shortened regeneration time of missing posterior portions of cut planarians by 22.4% and 31.3% (p<0.05), respectively. However, 50.0 μg/ml CAE induced lesions in regenerating planarians and was eventually shown to be lethal. Since CAE was able to reduce regeneration time in planarians, CAE may have a therapeutic application in hastening wound healing.
KEYWORDS: Anthocyanin; Ballatinao black rice; Dugesia hymanae; planarian; regeneration; wound healing.
Statement of Originality of Work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Quebral EPB & Medina PMB. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Case report
Veeranjaneyulu.P1,*.,MS(ENT),DLO, Ajay K Yadlapalli1,¥.,MS(ENT), Santosh K Batchu1,¥.,MS(ENT), Amjad Khan1,£.,MBBS, Rachana Chollangi1,£.,MBBS
Affiliation:
1Professor &Head,1,¥ Assistant Professor,1,£Junior resident, Department of ENT, GSL medical college, Rajahmundry, Andhra Pradesh, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of ENT, GSL medical college, Rajahmundry, Andhra Pradesh, India
Address reprint requests to
Dr. Veeranjaneyulu .P,
Professor and Head, Dept of ENT, GSL medical college, Rajanagaram, Rajahmundry, Andhrapradesh–533 296, India
Article citation:
Veeranjaneyulu P,Yadlapalli AK, Batchu SK, Khan A, Chollangi R. Recurrent nasopharyngeal angiofibroma: Endoscopic excision. J Pharm Biomed Sci. 2015; 05(02):104-109. Available at www.jpbms.info
ABSTRACT:
Juvenile Nasopharyngeal Angiofibroma (JNA) is a benign vascular neoplasm, the etiologic origin of which remains elusive. It comprises less than 0.5 % of all head and neck tumours. It occurs exclusively in males usually in the adolescent age.
Surgical excision, preferably with a preoperative embolisation,is the complete treatment of choice though recurrences are a rare possibility. Recurrence rate ranges from 6% to 27.5 %.
Endoscopic excision is done in some tumours of limited extension because of the benign nature of the neoplasm and its location in a cosmetically sensitive zone.
Here, we present a case of recurrent nasopharyngeal angiofibroma in a male patient of 16 yrs for whom endoscopic excision was done and there was no further recurrence, followed by the discussion on the etiologies, clinical features and the treatment modalities of nasopharyngeal angiofibroma.
KEYWORDS: Angiofibroma; Benign; Gammaknife surgery; Juvenile nasopharyngeal angiofibroma; Endoscopic excision; Nasopharynx; Recurrence; Vascular neoplasm; lasers; coblation; radiation therapy.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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Source of funding: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015. Veeranjaneyulu P,Yadlapalli AK, Batchu SK, Khan A, Chollangi R.. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Swarupjit Ghata1,*, Dharmendra Dugar2, Ranjit Kumar Mishra3, Ramji Khetri2,Tim Houghton T1
Affiliation:
1Third Year P.G Student, 2Associate professor, 3Professor and Head , Department of General Surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of General Surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
Address reprint requests to
Dr.Swarupjit Ghata,
Third Year P.G Student,
Department of General Surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
Article citation: Ghata S, Dugar D, Mishra R K, Khetri R, Tim HT. Study of port site complications in laparoscopic surgeries. J Pharm Biomed Sci. 2015; 05(02):134-138. Available at www.jpbms.info
ABSTRACT: Background: The approach to surgical treatment underwent a paradigm shift in 1987. Mouret performed the first human laparoscopic cholecystectomy in France in 1987 and now has become the gold standard treatment for patients suffering from cholelithiasis. Laparoscopic surgery became the standard care for many gynecological and surgical conditions with documented benefits and excellent outcome. In addition to the general complications of surgery and anesthesia, laparoscopy has unique complications relating to abdominal entry and surgical instrumentation. The early innovators and early adopters have identified such risks and complications associated with such procedures and analyzed them.
Material & method: It is a prospective study on complications of the port site in laparoscopic surgeries. The data collection for our study included patients from Hi-Tech medical college and hospital, Bhubaneswar, Odisha during the period June 2012- December 2014. All the complications encountered while creating ports in laparoscopic surgeries were studied. Complications related to faulty techniques and due to human error were taken into account and studied.
Results: In our study 100 different patients posted for laparoscopic surgeries in Hi-Tech medical college and hospital were studied to evaluate the port site complications in laparoscopic surgeries. In our study, out of 100 patients, 55 were male and 45 were female. Out of the 100 patients, there were 9 patients who had complications. Highest number of complications was seen in patients of 60 to 79 years of age. Thus, it was inferred that old patients who already had less immunity and who were suffering from other co-morbid conditions were at increased risk of complications from laparoscopic surgeries. Out of the 9 complications, 7 were post-operative, 1 occurred while maintaining the port during surgery and 1 occurred while creating port.
Conclusion: Laparoscopic surgery introduces to the new set of risks such as trocar injury, cardiovascular problems and damage to the bowel and major vessels that are rarely if ever encountered in open surgeries. Laparoscopic surgeries by inexperienced surgeons should be discouraged. All the training institutes should have a protocol to train, access, accredit the trained. All the surgeries should be recorded and analyzed to identify the cause of the complications noted and ways to avoid them. Working with a team makes a good sense.
KEYWORDS: Laparoscopy [Lap]; Port site complications; Trocar injury; bowel injury; vessel injury.
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Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Ghata S, Dugar D, Mishra R K, Khetri R, Tim HT. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Tim Houghton T1,*, Swarupjit Ghata1, Ranjit K Mishra1,¥, Jatasankar Mohapatra1,£, Dharmendra Dugar1,†
Affiliation:
1P.G Student,1¥Professor and HOD, 1£Professor, 1†M.S, Department of General surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of General surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
Address reprint requests to
Dr. Tim Houghton T
P.G Student, Department of General surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
Article citation:
Tim HT , Ghata S, Mishra RK , Mohapatra JS, Dugar D. Clinicopathological study on hollow viscus perforation. J Pharm Biomed Sci. 2015; 05(02):100-103. Available at www.jpbms.info
ABSTRACT:
Background: This work on hollow viscus perforation was done to evaluate the etiological factors, clinical parameters, laboratory and Radiological investigations in the diagnosis and to compare and evaluate post operative recovery till time of discharge.
Methods: This is a prospective study of 41 consecutive cases of hollow viscus perforation that were operated, from December 2012.
Results and conclusion: The commonest cause was of hollow viscus perforation was perforated duodenal ulcer. Most common symptom of was pain abdomen and next was vomiting. The mean duration of presenting complaint was 48 hours. Prolonged duration of presenting complaint was associated with increased occurrence postoperative complications and prolonged hospital stay. Use of x-ray abdomen along with USG of the abdomen helps in clinching the diagnosis in most cases. The most common complication was postoperative wound infection. From this study it is found that early presentation, early diagnosis, good preoperative resuscitation, timely surgical intervention, good post operative care is essential in all cases of hollow viscus perforation to reduce mortality.
KEYWORDS: Hollow viscus perforation; surgery; clinicopathological study.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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Source of funding: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015. Tim HT , Ghata S, Mishra RK , Mohapatra JS, Dugar D. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Hosuru Subramanya Supram*, Binita Koirala, Anupriya Rani, Shashir Gokhale,
Dharma raj Bhatta
Affiliation:-
Department of Microbiology, Manipal College of Medical Science, Pokhara, Nepal
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, Manipal College of Medical Science, Pokhara, Nepal
Address reprint requests to
Hosuru Subramanya Supram
Lecturer, Department of Medical Microbiology,
Manipal College of Medical Sciences, Pokhara, Nepal
Article citation:
Supram HS,Koirala B,Rani A, Gokhale S,Bhatta DR. Prevalence of intestinal parasitic infections in a tertiary care center at western Nepal: Five years retrospective study. J Pharm Biomed Sci.2015;05(02):154-159.Available at www.jpbms.info
ABSTRACT
Introduction: Parasitic infestations are the major causes of morbidity and mortality in developing countries like Nepal. It is an established fact that intestinal parasitic infections can lead to a number of adverse effects like anemia, reduced physical growth, abdominal colic, cholestasis, cholecystitis and pancreatitis. This study aims to assess the distribution pattern of intestinal parasites among patients attending Manipal Teaching Hospital.
Method: A retrospective study of results of stool samples analysis was carried out for intestinal parasite examination in a tertiary care Hospital, Pokhara. The records were collected from Microbiology Laboratory for a period of five years (2009 to 2013).
Result: Eight different types of parasites were encountered. The most common parasite Giardia lamblia, accounted for 140 (46.36%) followed by Entamoeba histolytica 53 (17.55%), Ancylostoma duodenale 30 (9.93%), Ascaris lumbricoides 22 (7.28%), Trichuris trichuria 22 (7.28%), Hymenolepsis nana 19 (6.29%), Taenia species9 (2.98%), and Strongyloides stercoralis 7 (2.32%). In our study the prevalence of intestinal parasitic infection is low.
Conclusion: The notable finding from this study is the high prevalence of Giardiasis. Since parasitic infestations are important public health problem, it is necessary to develop effective prevention and control strategies including health education and environmental hygiene.
KEYWORDS: Intestinal parasites, parasitic infestations, western Nepal.
Source of support: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
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Copyright © 2015. Supram HS,Koirala B,Rani A, Gokhale S,Bhatta DR. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.