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Original article
Guo Chen1, Zhenghua Ni2, Qingge Chen1, Xiongbiao Wang1,*
Affiliation:
1Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, PR China
2Central lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, PR China
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine &
2. Central lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China
Address reprint requests to
Xiongbiao Wang.
Central lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062,PR China
Article citation:
Wang et al. Gene silencing of IL-13 by siRNA aerosol inhalation inhibits asthma phenotype in the murine model of asthma. J Pharm Biomed Sci. 2015; 05(02):126-130. Available at www.jpbms.info
ABSTRACT:
Background: interleukin-13 has been shown to be a critical mediator of the asthma phenotype. It was reported that intravenously administration of the IL-13 siRNA in vivo reduced airway resistance significantly in the murine asthma model.
Objectives: This study was intended to investigate whether aerosol inhalation of IL-13 siRNA was effective in reduce IL-13 expression and thus attenuated asthma phenotype.
Methods: The ovalbumin (OVA)-challenged mouse model of asthma was used to assess the effect of IL-13 interference. Airway inflammation in lung tissue was assessed using hematoxylin and eosin (H&E). The mRNA expression of IL-13 gene was determined using real-time PCR.
Results: Aerosol inhalation of the IL-13 siRNA was shown to reduce the expression of IL-13 and reduced airway inflammation in lung tissues compared with control group.
Conclusion: Our results demonstrated the effectiveness of aerosol inhalation of IL-13 siRNA in preventing airway inflammation in vivo and suggested potential therapeutic applications.
KEYWORDS: IL-13; siRNA; asthma; aerosol inhalation.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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Source of funding: This work was supported by the Shanghai Science and Technology Committee (No.10411969100, No.12401900404).
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015. Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Hitesh R Ahir1,* Parimal H Patel1,†, Alka B Nerurkar1,¥
Affiliation:
1,*Tutor, 1,†Assistant Professor, 1,¥Professor and Head of Department, Department of Microbiology, GMERS, Medical College, Valsad, Gujarat, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Microbiology, GMERS, Medical College, Valsad, Gujarat, India
Address reprint requests to
Hitesh R Ahir*
Department of Microbiology, GMERS, Medical College, Valsad, Gujarat, India
Article citation: Ahir HR, Patel PH, Nerurkar AB. Intestinal Parasitic Infections in Patients attending Tertiary Care Hospital, Valsad, south Gujarat, India: A retrospective study. J Pharm Biomed Sci. 2015; 05(02):117-121. Available at www.jpbms.info
ABSTRACT:
Intestinal parasitic infections are widely prevalent in developing countries due to poor sanitation and inadequate personal hygiene. They are responsible for considerable morbidity and mortality.
Aim: The study was undertaken to assess the prevalence of Intestinal Parasitic Pathogens amongst patients attending a tertiary care hospital in district valsad, Gujarat.
Materials and Methods: Stool samples from 291 patients which included 120 females and 171 males were collected and screened using conventional saline and iodine wet mount and examined by direct microscopy.
Results: In our study the prevalence of intestinal parasitic infection was found to be 13.40%. E.histolytica (45.23%) and A. duodenale ( 26.19%) were the commonest parasites isolated, followed by A lumbricoides (11.90%), S stercoralis (11.90%) and Hymenolepis nana (4.76%). Infection rate was highest in females (15 %) than males (12.28%).The infestation was higher in the 21-30 years age group as compared to others. Multiple parasitic infection are detected in three stool samples(7%).
Conclusion: Helminthic infections are more common than protozoal. The present study reports that intestinal parasitic infection is prevalent in this area and this study also indicate the usefulness and easy to perform stool examination at very low cost.
KEYWORDS: Intestinal parasitic infections.
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Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Ahir HR, Patel PH, Nerurkar AB. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.