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Original article
Vaishali D. Kotasthane1,*, Dhananjay S Kotasthane1, G Koteeswaran1,Alok Mohanty2
Affiliation:
1Department of Pathology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Puducherry -607402, India
2Department of General Surgery, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Puducherry -607402, India
The name of the department(s) and institution(s) to which the work should be attributed:
Mahatma Gandhi Medical College and Research Institute,SBV University, Pillaiyarkuppam, Puducherry -607402, India
Address reprint requests to
Dr Vaishali D. Kotasthane, MD(Pathology),
Assistant professor, Department of Pathology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Puducherry-607402. India
Article citation: Kotasthane VD, Kotasthane DS, Koteeswaran G, Mohanty A. Clinicopathological study of colorectal tumors: A four year study in a rural tertiary care hospital in South India. J Pharm Biomed Sci. 2015; 05(02):139-146. Available at www.jpbms.info
ABSTRACT: Background: Colorectal tumors are thought to be comparatively uncommon in developing countries like India. Recent studies showed steady increase in incidence of colorectal cancers for developing countries. Aims: The aim of the present study was to evaluate histological types of colorectal tumors with respect to demographic data like age, gender distribution, anatomical site and grading.
Material and methods: This was a descriptive study of colorectal biopsies/specimens from May 2008 to May 2012 carried out in Department of Pathology, Mahatma Gandhi Medical College and Research Institute, a tertiary care hospital in Pondicherry. Relevant information like age, sex, site and clinical features was noted.
Statistical method: The data compiled was analyzed for demographic and biographical details like age, sex, site, clinical features, gross findings and light microscopic histopathological diagnosis. Descriptive analysis were reported as mean and standard deviation of continuous variables. Unpaired t test and chi-square test were used and value of p<0.05 was taken as statistically significant.
Results and conclusions: Total 68 colorectal tumors were observed, representing 6.7% of all tumors during study period. Out of these, 60.3% were malignant. Rectal carcinomas predominated over colonic carcinomas. For Colorectal carcinomas (CRCs), mean age at presentation was 61.8 years in males and 50.8 years in females which was significantly lower (p=0.0012) with Adenocarcinoma-NOS as commonest subtype. Early age at presentation in females, suggests that colonoscopic screening will be useful for early detection. Quantitative estimates of this study are of interest for future population-based studies and are potentially useful for targeting screening colonoscopy programmes.
KEYWORDS: Colorectal tumors; Adenocarcinomas; Polyps;Adenomas.
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Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Kotasthane VD, Kotasthane DS, Koteeswaran G, Mohanty A. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Tim Houghton T1,*, Swarupjit Ghata1, Ranjit K Mishra1,¥, Jatasankar Mohapatra1,£, Dharmendra Dugar1,†
Affiliation:
1P.G Student,1¥Professor and HOD, 1£Professor, 1†M.S, Department of General surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of General surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
Address reprint requests to
Dr. Tim Houghton T
P.G Student, Department of General surgery, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
Article citation:
Tim HT , Ghata S, Mishra RK , Mohapatra JS, Dugar D. Clinicopathological study on hollow viscus perforation. J Pharm Biomed Sci. 2015; 05(02):100-103. Available at www.jpbms.info
ABSTRACT:
Background: This work on hollow viscus perforation was done to evaluate the etiological factors, clinical parameters, laboratory and Radiological investigations in the diagnosis and to compare and evaluate post operative recovery till time of discharge.
Methods: This is a prospective study of 41 consecutive cases of hollow viscus perforation that were operated, from December 2012.
Results and conclusion: The commonest cause was of hollow viscus perforation was perforated duodenal ulcer. Most common symptom of was pain abdomen and next was vomiting. The mean duration of presenting complaint was 48 hours. Prolonged duration of presenting complaint was associated with increased occurrence postoperative complications and prolonged hospital stay. Use of x-ray abdomen along with USG of the abdomen helps in clinching the diagnosis in most cases. The most common complication was postoperative wound infection. From this study it is found that early presentation, early diagnosis, good preoperative resuscitation, timely surgical intervention, good post operative care is essential in all cases of hollow viscus perforation to reduce mortality.
KEYWORDS: Hollow viscus perforation; surgery; clinicopathological study.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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Source of funding: None
Competing interest / Conflict of interest
The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015. Tim HT , Ghata S, Mishra RK , Mohapatra JS, Dugar D. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original research article
Quebral, Elgin Paul B.† and Medina, Paul Mark B.*
Affiliation:
†MD-PhD in Molecular Medicine Program, College of Medicine, University of the Philippines Manila, Ermita, Manila, Philippines
*Faculty, Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Ermita, Manila, Philippines
Author contributions: Both authors contributed equally to this paper.
Address reprint requests to:
Medina, Paul Mark B.
Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Pedro Gil Street, Ermita, 1000 Manila, Philippines
Office Telephone/Fax: +632 526 0377; +632 526 4197
Article citation:
Quebral, EPB & Medina, PMB. Crude anthocyanin extract (CAE) from Ballatinao black rice hastens regeneration in Dugesia hymanae. J Pharm Biomed Sci. 2015; 05(02):147-153. Available at www.jpbms.info
ABSTRACT: Anthocyanins are a major group of flavonoids that have been examined for various medical and pharmacological applications. However, very few studies have explored the potential of anthocyanins for wound healing and regeneration. We investigated if a crude anthocyanin extract (CAE) from Ballatinao black rice (Oryza sativa L. tropical japonica) can affect regeneration in planarians (Dugesia hymanae). The planarians were transversely cut into two equal parts and were exposed to different concentrations of CAE. Our findings showed that 0.5 μg/ml and 5.0 μg/ml of CAE significantly shortened regeneration time of missing anterior portions of cut planarians by 7.7% (p<0.05). Additionally, 0.5 μg/ml and 5.0 μg/ml of CAE shortened regeneration time of missing posterior portions of cut planarians by 22.4% and 31.3% (p<0.05), respectively. However, 50.0 μg/ml CAE induced lesions in regenerating planarians and was eventually shown to be lethal. Since CAE was able to reduce regeneration time in planarians, CAE may have a therapeutic application in hastening wound healing.
KEYWORDS: Anthocyanin; Ballatinao black rice; Dugesia hymanae; planarian; regeneration; wound healing.
Statement of Originality of Work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Quebral EPB & Medina PMB. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
Arti Chopra1 DNB OBG, Nimarpreet Kaur2,* MD Physiology, Neeraj Aggarwal3 DNB Surgery, Hatinder Jeetsingh Sethi4 MD Internal Medicine
Affiliation:
1Consultant,Obstetrics and Gynaecology, Asian Multispecialty Hospital, Ludhiana 141002, Punjab, India
2Department of Physiology, SHKM, Govt Medical College, Nalhar, Mewat, Haryana 122107, India
3Consultant Surgery, Asian Multispecialty Hospital, Ludhiana 141002, Punjab, India
4Consultant, Department of internal medicine, Medanta, The Medicity, Gurgaon, Haryana 122001, India
The name of the department(s) and institution(s) to which the work should be attributed:
1.Asian Multispecialty Hospital, Ludhiana 141002, Punjab, India
2.SHKM, Govt Medical College, Nalhar, Mewat, Haryana 122107, India
3. Medanta, The Medicity, Gurgaon, Haryana 122001, India
Address reprint requests to
Dr. Arti Chopra.
Consultant, Asian Multispecialty Hospital, Ludhiana 141002,Pumjab,India or
Dr. Nimarpreet Kaur.
SHKM, Govt Medical College, Nalhar, Mewat, Haryana 122107, India
Article citation:
Chopra A, Kaur N, Aggarwal N, Sethi HJS. Endorette: A minimally invasive,cost effective & outpatient procedure for diagnosis of post menopausal bleeding. J Pharm Biomed Sci. 2015; 05(02):168-173. Available at www.jpbms.info
ABSTRACT: Menopause is defined as the permanent cessation of menses that occur after the cessation of ovarian function, which usually occur after the age of forty. The significance of post menopausal bleeding whatever slight it may be, should not be underestimated. All women with post menopausal bleeding must be examined & investigated. Malignancy must be ruled out.
Aims & Objectives: To study the diagnostic accuracy of Endorette in women with post-menopausal bleeding in endometrial diseases.
Materials & Methods: This study was conducted on 40 patients who presented with post menopausal bleeding. Patients underwent endometrial biopsy using outpatient endometrial sampling device – Endorette.The results were statistically analysed.
Results: In our study a total of forty (40) Post menopausal women were evaluated. Endorette was not possible in three (3) patients out of forty (40) because the endorette cannula could not be advanced into the uterine cavity due to cervical stenosis. The failure rate of the endorette procedure was 7.5% in our study. It could be successfully completed in 92.5% patients. Endorette is carried out as an outpatient procedure requiring no anaesthesia ,cost effective and diagnostic tool.
Conclusion: Outpatient endometrial sampling with endorette should be the first step of investigation in women with post menopausal bleeding to rule out uterine cause.
KEYWORDS: Menopause post menopausal Bleeding; Endometrial biopsy; Endocurette.
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Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: Department of Biotechnology, Ministry of Science & Technology, and Government of India to R.S
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Chopra A, Kaur N, Aggarwal N, Sethi HJS. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Guo Chen1, Zhenghua Ni2, Qingge Chen1, Xiongbiao Wang1,*
Affiliation:
1Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, PR China
2Central lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, PR China
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine &
2. Central lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China
Address reprint requests to
Xiongbiao Wang.
Central lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062,PR China
Article citation:
Wang et al. Gene silencing of IL-13 by siRNA aerosol inhalation inhibits asthma phenotype in the murine model of asthma. J Pharm Biomed Sci. 2015; 05(02):126-130. Available at www.jpbms.info
ABSTRACT:
Background: interleukin-13 has been shown to be a critical mediator of the asthma phenotype. It was reported that intravenously administration of the IL-13 siRNA in vivo reduced airway resistance significantly in the murine asthma model.
Objectives: This study was intended to investigate whether aerosol inhalation of IL-13 siRNA was effective in reduce IL-13 expression and thus attenuated asthma phenotype.
Methods: The ovalbumin (OVA)-challenged mouse model of asthma was used to assess the effect of IL-13 interference. Airway inflammation in lung tissue was assessed using hematoxylin and eosin (H&E). The mRNA expression of IL-13 gene was determined using real-time PCR.
Results: Aerosol inhalation of the IL-13 siRNA was shown to reduce the expression of IL-13 and reduced airway inflammation in lung tissues compared with control group.
Conclusion: Our results demonstrated the effectiveness of aerosol inhalation of IL-13 siRNA in preventing airway inflammation in vivo and suggested potential therapeutic applications.
KEYWORDS: IL-13; siRNA; asthma; aerosol inhalation.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
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Source of funding: This work was supported by the Shanghai Science and Technology Committee (No.10411969100, No.12401900404).
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015. Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.