DocumentsDate added
Research article
WEN Shaohong1,†, OU Jinlai2,†, LUO Rui1, LIANG Wenli1, OUYANG Ping1,
ZENG Fen1, CHEN Yanwen1, XU Zhenxia1, ZHAO Wen1, LI Sha1,*
Affiliation:
1Department of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou 510632, China
2Department of Preparation, Sunshinelake Pharma co., LTD., Shenzhen 523871, Guangdong, China
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou 510632, China
2. Department of Preparation, Sunshinelake Pharma co., LTD., Shenzhen 523871, Guangdong, China
† Authors contributions: Both WEN Shaohong and OU Jinlai contributed equally to this work.
Address reprint requests to
LI Sha.
Department of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou 510632, China or at tlisha@jnu.edu.cn
Article citation:
Shaohong W, Jinlai OU,R Luo, Wenli L, Ping O, Fen Z,Yanwen C,et al. Preparation and release behavior of pectin nanoparticles loading doxorubicin. J Pharm Biomed Sci. 2015; 05(05):385-393. Available at www.jpbms.info
ABSTRACT: Doxorubicin (DOX) is a highly efficient and broad-spectrum antitumor drug in the treatment of solid tumors. However, DOX can lead to a wide range of biochemical effects on human including inevitable toxicity to the normal tissues which has limited its clinical application. Nanoparticles, as a new kind of vehicle for antitumor drug delivery, can greatly enhance the theraputic effect and reduce the drug toxicity. In this work, we chose pectin (PEC) as carrier material to prepare DOX loading nanoparticles. The blank PEC nanoparticles (PEC-NPs) were prepared by emulsification-ionotropic gelation method and the formulation was optimized by central composite design and response surface method. The drug was loaded in PEC-NPs to obtain DOX loading pectin nanoparticles (DOX-PEC-NPs) by adsorption. The drug loading capacity of PEC-NPs was chiefly affected by ratio of DOX to PEC. The time, pH value and temperature of adsorption showed some influence on drug loading. Both PEC-NPs and DOX-PEC-NPs were sphere-like in shape with a mean diameter of (266.93 ± 1.70) nm and (287.70 ± 1.21) nm, respectively. The entrapment efficiency and drug loading rate of DOX-PEC-NPs was (91.71 ± 0.50)% and (17.17 ± 0.24) %, respectively. Compared with DOX, DOX-PEC-NPs showed apparent sustained release behavior with a burst release, which followed the double exponential diphase kinetics equation well.
KEYWORDS: Doxorubicin; Pectin; Nanoparticles; Drug loading; Drug release.
REFERENCES
1.Gong LS, Zhang YD, Zhou SB. The Biodistribution of magnetic albumin nanoparticles containing doxorubicin in rats[J]. China Journal of Modern Medicine. 2001;11(3):8-10.
2.Menon SK, Mistry BR, Joshi KV, et a1. Analytical detection and method development of anticancer drug Gemcitabine HCl using gold nanoparticles[J]. Spectrochim Acta A Mol Biomol Spectrosc. 2012; 10(94A):235-242.
3.Stein W, Bates S, Fojo T. Intractable cancers: the many faces of multidrug resistance and the many targets it presents for therapeutic attack. Current Drug Targets. 2004;5(4):333-346.
4.Kingsley JD, Dou H, Morehead J, et al. Nanotechnology: a focus on nanoparticles as a drug delivery system[J]. Journal of Neuroimmune Pharmacology. 2006;1(3):340-350.
5.Joseph MM, Aravind SR, George SK, et al. Anticancer activity of galactoxyloglucan polysaccharide-conjugated doxorubicin nanoparticles: Mechanistic insights and interactome analysis. Eur J Pharm Biopharm. 2015; 93:183-195.
6.You JO. Efficient gene delivery with calcium-alginate nanoparticles[D]. University of Southern California. 2006.
7.Mao SJ, Hou SX, Zhang LK. Preparation and liver targeting properties of glycyrrhizic acid surface modified Valaciclovir albumin nanoparticles[J]. Journal of Biomedical Engineering. 2004;21(4):570-574.
8.Chen J, Hu Q, Hou ZQ, et al. Chitosan nanoparticles prepared by ion induction combined with chemical crosslinking method and preliminary studies about its being a targeting control-released carrier for drugs[J]. Chinese Pharmaceutical Journal. 2010; (18): 1400-1403.
9.Xu H, Hu FQ, Ying XY, et al. Preparation of evaluation of in vitro and in vivo of alginate nanoparticles loading insulin[J]. Chinese Pharmaceutical Journal. 2006; 41(6): 434-437.
10.Zhou XY, Shen Q, Li ML, et al. Preparation of Saussurea nanoparticles by multiple emulsion method[J]. Lishizhen Medicine and Materia Medica Research. 2010; 21(7):1726-1727.
11.Vanderwaal RP, Griffith CL, Wright WD. Delaying S-phase progression rescues cells from heat-induced S-phase hypertoxicity[J]. Journal of Cellular Physiology. 2001;14(2):81-82.
12.Taheri A, Dinarvand R, Atyabi F, et al. Targeted delivery of methotrexate to tumor cells using biotin functionalized methotrexate human serum albumin conjugated nanoparticles[J]. J Biomed Nanotechnol. 2011;7(6):743-753.
13.Widder KJ, Marino PA, Morris RM. Selective targeting of magnetic albumn microspheres to the yoshida Sarcoma: Ultrastructural evaluation of microsphere disposition[J]. European Journal of Cancer & Clinical Oncology. 1983;(1):96-98.
14.Brusentsov NA, Gogosov VV, Brusentsova TN. Evaluation of ferromagnetic fluids and suspensions for the site-specific radiofrequency-induced hyperthermia of MX11 sarcoma cells in vitro[J]. Journal of Magnetism and Magnetic Materials. 2001;11(2):934-940. 15.Ashford M, Fell J, Attwood D. Enhanced pectin degradation in the presence of calcium-implications for colonic delivery[J]. Proc Int Symp Controlled Release Bioact Mater. 1994; 21:750-751.
16.Sriamornsak P. Chemistry of pectin and its pharmaceutical uses: A review[J]. Silpakorn University International Journal. 2003;3(1-2):206-228.
17.Mishra R, Banthia A, Majeed A. Pectin based formulations for biomedical applications: A review[J]. Asian Journal of Pharmaceutical & Clinical Research. 2012;5(4):1-7.
18.Liu L, Fishman ML, Kost J, et al. Pectin-based systems for colon-specific drug delivery via oral route[J]. Biomaterials. 2003;24(19):3333-3343.
19.Sriamornsak P. Application of pectin in oral drug delivery[J]. Expert opinion on drug delivery. 2011; 8(8):1009-1023.
20.Yoshimura T, Sengoku K, Fujioka R. Pectin-based surperabsorbent hydrogels crosslinked by some chemicals: Synthesis and characterization[J]. Polymer bulletin. 2005; 55(1-2):123-129.
21.Cheng K, Lim L-Y. Insulin-loaded calcium pectinate nanoparticles: Effects of pectin molecular weight and formulation pH[J]. Drug development and industrial pharmacy. 2004;30(4):359-367.
22.Glinsky VV, Raz A. Modified citrus pectin anti-metastatic properties: One bullet, multiple targets[J]. Carbohydrate research. 2009; 344(14):1788-1791.
23.Li S, Wang XT, Zhang XB, et al. Studies on alginate-chitosan microcapsules and renal arterial embolization in rabbits[J]. Journal of Controlled Releaase. 2002;84(3):87-98.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Shaohong W,Jinlai OU,R Luo,Wenli L,Ping O,Fen Z,Yanwen C, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Lakshminarasimhaiah* and Rakesh Kumar Meel
Affiliation:
Goenka College of Pharmacy, Village: Ghassu, NH-11, PO: Khuri Bari, Lachhmangarh, Sikar District, Rajasthan, India-332313
The name of the department(s) and institution(s) to which the work should be attributed:
Goenka College Of Pharmacy, Vill: Ghassu, NH-11, PO: Khuri Bari, Lachhmangarh, Sikar District, Rajasthan, India-332313
Address reprint requests to
Lakshminarasimhaiah,
Goenka College OF Pharmacy, Vill: Ghassu, NH-11, PO: Khuri Bari, Lachhmangarh, Sikar District, Rajasthan, India-332313
Article citation: Lakshminarasimhaiah, Meel RK. Isolation of new phytoconstituents from Actiniopteris radiata. J Pharm Biomed Sci. 2015; 05(05):436-439. Available at www.jpbms.info
ABSTRACT:
Two new phytoconstituents have been isolated from petroleum ether extract of the whole plant of Actiniopteris radiata. The petroleum ether extract was subjected to column chromatography and isolated the phytoconstituents. Two new phytoconstituents were characterised by TLC, IR, UV spectral analysis, NMR and Mass spectra. Compound 1 is Dec-3-enyl, 2-(heptyloxy)-1-mercaptpoethyl terephthalate and compound 2 is 1-(Phenylethyl butanoate ether), 2-(3-ene heptanoate) ethane.
KEYWORDS: Actiniopteris radiata; Column chromatography; Polypodiaceae; Terephthalate.
REFERENCES
1.Bhambie S and George C. X. Studies in Pteridophytes. Cur Sci 1972; 41(7), 258-260.
2.Bhambie S, Tiwari H. P and George C. X. Chemical analysis Actiniopteris radiata. Cur Sci 1972;41(21): 788.
3.Taneja S. C and Tiwari H. P. Chemical constituents of Actiniopteris radiata. Cur Sci 1974; 43(23):749-750.
4.Yoshihiro Mimaki, Kazuki Watanabe, Yuka Ando, Chiseko Sakuma and Yutaka Sashida. Flavonol glycosides and steroidal saponins from the leaves of Cestrum nocturnum and their cytotoxicity. J Nat Prod 2001; 64:17-22.
5.Chien-Chih Chen, Yu-Lin Huang, Chu-Wen Wang and Jun-Chih Ou. Water soluble glycosides from Ruta graveolens. J Nat Prod 2001; 64:990-992.
6.Alsuko Itoh, Takao Tanahashi, Naotaka Nagakura, Yukiko Takenaka, Cheng-Chang Chen and Jury Pelletier. Flavonoid glycosides from Adina racemosa and their inhibitory activities on eukaryotic protein synthesis. J Nat Prod 2004; 67:427-431.
7.Nabil Semmar, Bernard Fenet, Katia Gluchoff-Fiasson, Aurangzeb Hasan and Maurice Jay. Four new flavonol glycosides from the leaves of Astragalus caprinus. J Nat Prod 2002;65:576-579.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Lakshminarasimhaiah, Meel RK. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.