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Research article
LIANG Wenli1,†, LI Linlin2,†, LUO Rui1, XU Weihao1, TANG Yu1, XU Zhenxia1, ZHAO Wen1,NI Peihong3, LI Sha1,*
Affiliation:
1Department of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou 510632, China
2The Fourth People's Hospital of Chenzhou, Chenzhou 423000, Hunan, China
3Guangzhou Toplink Gene Bio-Engineering Co. Ltd.,Guangzhou 510000, China
The name of the department(s) and institution(s) to which the work should be attributed:
1.Department of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou 510632, China
2.The Fourth People's Hospital of Chenzhou, Chenzhou 423000, Hunan, China
† Authors contributions: Both LIANG Wenli and LI Linlin contributed equally to this work.
Address reprint requests to
LI Sha.
Department of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou 510632, China
Article citation: W Liang, L Li, R Luo, W Xu, Y Tang, Z Xu, et al. Preparation and characterization of diclofenac sodium sustained-release solid dispersion. J Pharm Biomed Sci. 2015; 05(05):394-402.Available at www.jpbms.info
Research article
WEN Shaohong1,†, OU Jinlai2,†, LUO Rui1, LIANG Wenli1, OUYANG Ping1,
ZENG Fen1, CHEN Yanwen1, XU Zhenxia1, ZHAO Wen1, LI Sha1,*
Affiliation:
1Department of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou 510632, China
2Department of Preparation, Sunshinelake Pharma co., LTD., Shenzhen 523871, Guangdong, China
The name of the department(s) and institution(s) to which the work should be attributed:
1. Department of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou 510632, China
2. Department of Preparation, Sunshinelake Pharma co., LTD., Shenzhen 523871, Guangdong, China
† Authors contributions: Both WEN Shaohong and OU Jinlai contributed equally to this work.
Address reprint requests to
LI Sha.
Department of Pharmaceutics, College of Pharmacy, Jinan University, Guangzhou 510632, China or at tlisha@jnu.edu.cn
Article citation:
Shaohong W, Jinlai OU,R Luo, Wenli L, Ping O, Fen Z,Yanwen C,et al. Preparation and release behavior of pectin nanoparticles loading doxorubicin. J Pharm Biomed Sci. 2015; 05(05):385-393. Available at www.jpbms.info
ABSTRACT: Doxorubicin (DOX) is a highly efficient and broad-spectrum antitumor drug in the treatment of solid tumors. However, DOX can lead to a wide range of biochemical effects on human including inevitable toxicity to the normal tissues which has limited its clinical application. Nanoparticles, as a new kind of vehicle for antitumor drug delivery, can greatly enhance the theraputic effect and reduce the drug toxicity. In this work, we chose pectin (PEC) as carrier material to prepare DOX loading nanoparticles. The blank PEC nanoparticles (PEC-NPs) were prepared by emulsification-ionotropic gelation method and the formulation was optimized by central composite design and response surface method. The drug was loaded in PEC-NPs to obtain DOX loading pectin nanoparticles (DOX-PEC-NPs) by adsorption. The drug loading capacity of PEC-NPs was chiefly affected by ratio of DOX to PEC. The time, pH value and temperature of adsorption showed some influence on drug loading. Both PEC-NPs and DOX-PEC-NPs were sphere-like in shape with a mean diameter of (266.93 ± 1.70) nm and (287.70 ± 1.21) nm, respectively. The entrapment efficiency and drug loading rate of DOX-PEC-NPs was (91.71 ± 0.50)% and (17.17 ± 0.24) %, respectively. Compared with DOX, DOX-PEC-NPs showed apparent sustained release behavior with a burst release, which followed the double exponential diphase kinetics equation well.
KEYWORDS: Doxorubicin; Pectin; Nanoparticles; Drug loading; Drug release.
REFERENCES
1.Gong LS, Zhang YD, Zhou SB. The Biodistribution of magnetic albumin nanoparticles containing doxorubicin in rats[J]. China Journal of Modern Medicine. 2001;11(3):8-10.
2.Menon SK, Mistry BR, Joshi KV, et a1. Analytical detection and method development of anticancer drug Gemcitabine HCl using gold nanoparticles[J]. Spectrochim Acta A Mol Biomol Spectrosc. 2012; 10(94A):235-242.
3.Stein W, Bates S, Fojo T. Intractable cancers: the many faces of multidrug resistance and the many targets it presents for therapeutic attack. Current Drug Targets. 2004;5(4):333-346.
4.Kingsley JD, Dou H, Morehead J, et al. Nanotechnology: a focus on nanoparticles as a drug delivery system[J]. Journal of Neuroimmune Pharmacology. 2006;1(3):340-350.
5.Joseph MM, Aravind SR, George SK, et al. Anticancer activity of galactoxyloglucan polysaccharide-conjugated doxorubicin nanoparticles: Mechanistic insights and interactome analysis. Eur J Pharm Biopharm. 2015; 93:183-195.
6.You JO. Efficient gene delivery with calcium-alginate nanoparticles[D]. University of Southern California. 2006.
7.Mao SJ, Hou SX, Zhang LK. Preparation and liver targeting properties of glycyrrhizic acid surface modified Valaciclovir albumin nanoparticles[J]. Journal of Biomedical Engineering. 2004;21(4):570-574.
8.Chen J, Hu Q, Hou ZQ, et al. Chitosan nanoparticles prepared by ion induction combined with chemical crosslinking method and preliminary studies about its being a targeting control-released carrier for drugs[J]. Chinese Pharmaceutical Journal. 2010; (18): 1400-1403.
9.Xu H, Hu FQ, Ying XY, et al. Preparation of evaluation of in vitro and in vivo of alginate nanoparticles loading insulin[J]. Chinese Pharmaceutical Journal. 2006; 41(6): 434-437.
10.Zhou XY, Shen Q, Li ML, et al. Preparation of Saussurea nanoparticles by multiple emulsion method[J]. Lishizhen Medicine and Materia Medica Research. 2010; 21(7):1726-1727.
11.Vanderwaal RP, Griffith CL, Wright WD. Delaying S-phase progression rescues cells from heat-induced S-phase hypertoxicity[J]. Journal of Cellular Physiology. 2001;14(2):81-82.
12.Taheri A, Dinarvand R, Atyabi F, et al. Targeted delivery of methotrexate to tumor cells using biotin functionalized methotrexate human serum albumin conjugated nanoparticles[J]. J Biomed Nanotechnol. 2011;7(6):743-753.
13.Widder KJ, Marino PA, Morris RM. Selective targeting of magnetic albumn microspheres to the yoshida Sarcoma: Ultrastructural evaluation of microsphere disposition[J]. European Journal of Cancer & Clinical Oncology. 1983;(1):96-98.
14.Brusentsov NA, Gogosov VV, Brusentsova TN. Evaluation of ferromagnetic fluids and suspensions for the site-specific radiofrequency-induced hyperthermia of MX11 sarcoma cells in vitro[J]. Journal of Magnetism and Magnetic Materials. 2001;11(2):934-940. 15.Ashford M, Fell J, Attwood D. Enhanced pectin degradation in the presence of calcium-implications for colonic delivery[J]. Proc Int Symp Controlled Release Bioact Mater. 1994; 21:750-751.
16.Sriamornsak P. Chemistry of pectin and its pharmaceutical uses: A review[J]. Silpakorn University International Journal. 2003;3(1-2):206-228.
17.Mishra R, Banthia A, Majeed A. Pectin based formulations for biomedical applications: A review[J]. Asian Journal of Pharmaceutical & Clinical Research. 2012;5(4):1-7.
18.Liu L, Fishman ML, Kost J, et al. Pectin-based systems for colon-specific drug delivery via oral route[J]. Biomaterials. 2003;24(19):3333-3343.
19.Sriamornsak P. Application of pectin in oral drug delivery[J]. Expert opinion on drug delivery. 2011; 8(8):1009-1023.
20.Yoshimura T, Sengoku K, Fujioka R. Pectin-based surperabsorbent hydrogels crosslinked by some chemicals: Synthesis and characterization[J]. Polymer bulletin. 2005; 55(1-2):123-129.
21.Cheng K, Lim L-Y. Insulin-loaded calcium pectinate nanoparticles: Effects of pectin molecular weight and formulation pH[J]. Drug development and industrial pharmacy. 2004;30(4):359-367.
22.Glinsky VV, Raz A. Modified citrus pectin anti-metastatic properties: One bullet, multiple targets[J]. Carbohydrate research. 2009; 344(14):1788-1791.
23.Li S, Wang XT, Zhang XB, et al. Studies on alginate-chitosan microcapsules and renal arterial embolization in rabbits[J]. Journal of Controlled Releaase. 2002;84(3):87-98.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Shaohong W,Jinlai OU,R Luo,Wenli L,Ping O,Fen Z,Yanwen C, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Lavanya Kollapu*, Suseela Kumari Pamu
Affiliation:
Assistant professor, Department of Microbiology, Rangaraya Medical College, GGH Campus, Kakinada, Andhrapradesh. 533001, India
The name of the department(s) and institution(s) to which the work should be attributed:
Assistant Professor, Department of Microbiology, Rangaraya Medical College, GGH Campus, Kakinada, Andhrapradesh. 533001, India
Address reprint requests to
Dr. Lavanya Kollapu.
Assistant Professor, Department of Microbiology, Rangaraya Medical College, GGH Campus, Kakinada, Andhrapradesh 533001, India
Article citation:
Kollapu L, Pamu SK. Seroprevalence of human brucellosis among high risk population. J Pharm Biomed Sci. 2015;05(05):419-423. Available at www.jpbms.info
ABSTRACT: Brucellosis is a zoonotic disease of worldwide distribution and has great economic concern. It is a contagious disease of ruminant animals but also effects human beings. The duration of the disease can vary from a few weeks to many months. Materials and methods: A total number of 200 samples tested for RBPT and STAT by using phenol saline as diluent to know the IgG titre and 2-mercapto ethanol was used as diluents to know the IgM titre. ELISA test was performed for all positive samples in RBPT, to know the presence of IgM antibody. All the results were analysed statistically.
Results: Of the200 serum samples, highest proportion of positive cases were slaughter house workers 21.05% and lowest proportion was with PUO cases 6.97%.case distribution according to positivity of RBPT and STAT highest proportion in slaughter house workers 13.5% and lowest proportion in PUO cases 6.97%.
Conclusion: Prevention of human brucellosis focuses mainly on elimination of infection among farm animals. Cooperation is recommended between public health and veterinary officials to overcome the failure of controlling disease among both animals and humans.
KEYWORDS: Human Brucellosis; RBPT; STAT; ELISA; IgM Antibodies.
REFERENCES
1.Charif A, Moullok B, Douclock A. Arch de Inst Past Algeria 1996; 55:14.
2.Young EJ: An overview of human brucellosis.Clin Infect Dis 1995 Aug :21(2):283-9:quiz 290.
3.Dalrymple- Champneys, W. 1960. Brucella isolated from reindeer (in Russian). Trudy Vsyesoyuz Inst Eksp Vet. 27.24-31.
4.Zammit, T. 1905. A preliminary note on the examination of blood of goats suffering from Mediterranean Fever, Report of the commission on Mediterranean Fever, part III. London: Harrison and Sons, 83.
5.Horrocks, W.H. and Kennedy, J.C. 1906. Goats as a means of propagation of Mediterranean Fever, Report of the commission on Mediterranean Fever, part IV. London: Harrison and Sons, 37-69.
6.Young, E.J. 1983, Human brucellosis. Rev Infect Dis, 5, 821-42.
7.Sadusk, J.F., Browne, A.S and Eorn, J.L., 1957. Brucellosis in man resulting from Brucella abortus (strain19) vaccine. JAMA. 164, 1325-7.
8.Wong, D.H. and Chow, C.H. 1937. Group agglutinins of Brucella abortus and Vibrio cholera. China Med J.52, 591-4.
9.Evalution of often immune capture agglutination test for sero diagnosis for human brucellosis-Antonio O Orduna etal., 2000.
10.Colmenero JD, Reguera JM, Martos F, Sa’nchez- DeMora D, Delgado M, Causse M et.al., Complications associated with Brucella melitensis infection. A study of 530 cases Medicine (Baltimore) 1996; 75; 195-211.
11.Young EJ: An overview of human brucellosis. Clin infec Dis 1995 AUG; 21(2):283-9; Quiz 290.
12.Baldi, PC.,Araj, GF., Racaro, GC., Wallach, JC. & Fossati, CA(1999). Detection of antibodies to Brucella cytoplasmic proteins in the cerebrospinal fluid of patients with neurobrucellosis. Clin Diagn Lab Immunol 6,756-759.
13.FAO/WHO. 1986. Report, Joint FAO/WHO Expert committee on Brucellosis, Technical Report Series No.740. Geneva:WHO.
14.Mahgoub , kambel AM , 1983 G.A. Jamjom and M . N. H Chowduary 1983. Brucellosis in Riyadh Saudi Arabia A Microbiological and clinical study. Trans - R -Soc - Trop . Med -Hyg 77:820-824.
15.Mousa, A.R.M., Elhag, K.M., et al.1988. The nature of human brucellosis in Kuwait: study of 379 cases. Rev Infect Dis, 10;211-17.
16.Dajani YF, Masoud AA, Barakat HF. Epidemiology and diagnosis of human brucellosis in Jordan. J.Trop.Med.Hyg.(1989-92(3):209-14.
17.Smits HL, Kadri SM.Brucellosis in India. A deceptive infectious disease. Indian J Med Res. 2005; 122:375-384.
18.Diaz R. And Moriyon, I. 1989.Laboratory techniques in the diagnosis. In Young, E.J. and Corbel, M.J.(eds). Brucellosis: clinical and laboratory aspects. Boca Raton, FL: CRC Press.73-83.
19.Ariza, J., Pellicer, T.,et al. 1992. Specific antibody profile in human brucellosis. Clin Infect Dis, 14, 131-40. 20 .Annapurna S. Agasthya et al. Seroprevalence study of Human Brucellosis by Conventional tests and Indigenous Indirect Enzyme-linked immunosorbent assay.2012.
21.Kapoor PK .sharma SN Rao KL. Seroprevalence of brucellosis in goats and human being in BiANER,(Rajasthan). Ind J. Comp microbial Immunol infect Dis 1985;6; 96-101.
22.Cifti C, Ozturk F, Oztekin A, Karaoglan H, Saba R, Gultekin M, Mamikoglu L. Comparison of the serological tests used for the laboratory diagnosis of brucellosis. Microbiyol Bul 2005 Jul; 39 (3)291-9.
23.Kumar p .Singh D. Barbuddhe SB.Seroprevalence of brucellosis among abattoir personnel in Delhi. J.Commun Dis 1997; 29:131-7.
24.Handa R, Singh S, Singh N, Wali JP. Brucellosis in North India results of a prospective study. J.Commun Dis 1998; 30 85-7.
25.M.O.Gad EL- Rab and A.M.kambal,”of a brucella enzyme immunoassay test (ELISA) in comparison with bacteriological culture and agglutination, Journal of Infection 36;Supplement 2:197-201.
26.Soliman, S.A., 1998 studies on brucellosis in farm animals with reference to public health importance in suez canalDistrict. P.h.D . thesis, for Med - Suez - canal Univ
27.Kumar P, Singh D, Barbuddhe SB. Sero prevalence of brucellosis among abattoir personnel in Delhi. J Commun Dis 1997; 29 :131-7.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Kollapu L, Pamu SK. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original Article
Jagyasmita Balasamant1,*, Rajesh Kumar Padhy1,¥, Amruta Kar1,±,Rakesh Kumar Giri1, Pallavi Agarwal1
Affiliation:
1IInd year P.G Student,1,¥Associate professor, Department of Otorhinolaryngology,
1,±Assistant professor, Department of microbiology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Otorhinolaryngology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
Address reprint requests to
Dr. Jagyasmita Balasamant.
IInd year P.G Student,
Department of Otorhinolaryngology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India
Article citation:
Balasamant J, Padhy RK, Kar A, Giri RK, Agarwal P. Study of outcomes of tympanoplasty and effect of bacteria in quiescent stage of mucosal Type of chronic otitis media. J Pharm Biomed Sci. 2015; 05(05):424-428. Available at www.jpbms.info
ABSTRACT:
Background: The aims of this study were to determine whether any organism does exist in middle ear cavity in mucosal type of chronic otitis media, if so isolate their types and assess their effect on graft uptake results.
Materials and Methods: Patients from 10 years of age and above with diagnosis of chronic otitis media mucosal type were included for tympanoplasty. Swab was collected from middle ear cavity for direct smear examination and culture prior to surgery in operation theatre and inoculated into Blood agar, Chocolate agar and Mac Conkey agar within half an hour. The isolates were identified with the use of standard bacteriological technique as described by American Society of Microbiology. All patients underwent tympanoplasty.
Observation: Graft uptake results were assessed after 6 weeks. 70 patients were enrolled for study. Majority of the patients were of the age group 11-20 years (42%). Among which male were more common with 71.4%. Bacteria were isolated from 43 cases (61.4%). Pseudomonas aeruginosa were isolated in 18(41.8%), Staphylococcus aureus in 11(25.7%), Klebsiella in 6(13.9%), Proteus in 3(6.9), E. coli in 3(6.9%) and Acinetobacter in 2(4.7%). All patients had undergone tympanoplasty. Graft uptake was (87.1%). All 9 failure cases had residual perforation. In bacteria isolated group, 37 out of 43 had graft uptake.
Conclusion: Success rate of tympanoplasty in quiescent stage was comparable with dry ear. Pseudomonas aeruginosa was the most common organism. Presence of bacteria in middle ear during surgery did not increase the risk of graft failure.
KEYWORDS: Bacteriology; Chronic otitis media; Tympanoplasty.
REFERENCES
1.Browning GG, Merchant SN, Kelly G, Swan IR, Canter R, McKerrows WS. Chronic otitis media. Scotts-Brown’s Otorhinolaryngology, Head and Neck Surgery, 7th ed .London: Edward Arnold publisher Ltd; 2008.3345-3395.
2.Tuli IP. Chronic suppurative otitis media. Textbook of Ear, Nose and Throat, 1st ed. New Delhi: Jaypee Brothers; 2005.58-61.
3.Mawson S, Ludman H. Disease of the Ear. A Textbook of Otology.
4.Krishnan A, Reddy EK. Tympanoplasty with or without mastoidetomy; Indian J of Otorhinolaryngology 2009; 266:819-22.
5.Brown C, Yi Q, Mc Carty D J, Briggs RJS. Success rate following myringoplasty at the Royal Victorian Eye and Ear Hospital. The Australian Journal of Otolaryngology 2002,29:606-11.
6.Caylan R, Titiz A, Falcioni M. Myringoplasty in children: factors influencing surgical outcome. Otolaryngol Head Neck Surg 1998; 118:709-713.
7.Browning GG, Picozzy GL, Sweeney G and Galder I.T: Role of anaerobe in chronic otitis media. Clin Otolaryngol 1983; 8:47-51.
8.Carlin WV, Lesser TH, John DG. Systemic antibiotic prophylaxis and reconstructive ear surgery. Clin Otolaryngol 1987; 12: 441–446.
9.Poorey VK, Iyer A. A Study of Bacteriological Flora in Chronic Suppurative Otitis Media and its Clinical Significance. Indian Journal of Otology and Head and Neck Suegery 2002; 54: 91-95.
10.Gupta V, Gupta A,. Chronic Suppurative Otitis Media: An Aerobic Micribiological Study. Indian J of Otology 1998; 4: 79-82.
11.Raj A, Tripathi V. Review of patient undergoing wet myringoplasty. Indian Journal of Otology 1999; 5(3): 134-136.
12.Vartianen E, Nutinen J. Success and pitfalls in myringoplasty: follow up study of 404 cases. Am J Otol 1993; 14(3): 301-305.
13.Sinha P. Aerobic bacteriological study of chronic otitis media. Indian Journal of Otology 1999; 5: 203-206.
14.Geradorff M, Garin P, Decat M, Jauntegui M. Myringoplasty long term results in adults and children. Am J Otol 1995; 16: 532-535.
15.Willium H, Slattery III. Pathology and clinical course of inflammatory disease of middle ear. Glasscock-Shambaugh Surgery of the ear. 5th ed. New Delhi: Elsvier India; 2003: 422-433.
16.Sharma S, Regan HS, Goyal A, Jha AK, Upadhya S, Mishra SC. Bacteriological profile in chronic suppurative otitis media in Eastern Nepal. Trop Doct . 2004; 34(2):102-104.
17.Vijaya D, Nagarathnam T. Microbiological study of chronic suppurative otitis media. Indian Journal of Otology .1998; 4: 172-174.
18.Fatma A, Assiry S, Zakzour S. Microbiological evaluation and aspect on management of chronic suppurative otitis media in Riyadh. Indian Journal of Otology 1998; 4: 115-120.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Balasamant J, Padhy RK, Kar A, Giri RK, Agarwal P. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
AL- Bayati LAA*
Affiliation:
Department of Biology, College of Science, Babylon, Hilla P.O.Box 4. Iraq
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Biology, College of Science, Babylon, Hilla P.O.Box.4. Iraq
Address reprint requests to
Lubna A. A. AL- Bayati.
Department of Biology, College of Science, Babylon, Hilla P.O.Box.4. Iraq
Article citation:
AL- Bayati LAA. The Immune Function of Iraqi Women‘s on Contraceptives intake. J Pharm Biomed Sci. 2015; 05(05):385-393. Available at www.jpbms.info
ABSTRACT: The complement C3 and C4 fractions as well as the cytokines Interferon and Interleukine 6 were estimated in serum samples of women during contraceptive intake. These estimations were made in an attempt to evaluate them as a diagnostic battery for detection of immune status in these women's.
The C3 concentration means were; 154.553 ± 12.084, 149.816 ± 10.423, 130.216± 6.147, 159.966± 8.583 and a 8.125± 21.570 mg/dl for pills , depot injection, IUDC, nonusing and virgin controls, respectively.
In comparison C4 levels were ; 42.825± 4.293, 34.583 ± 2.801, 32.466± 3.688, 33.025± 3.025 and 26.441± 6.680 mg/dl for pills, injection, IUDC, nonusing and virgin controls respectively. The interferone gama concentration were; 55.169±8.962, 80.266±3.630, 66.171±3.973, 26.499± 3.482 and 74.760±2.930 pg/ml accordingly.
The interleukine 6 concentration means were found as; 28.399± 8.517 for pills, 36.380±8.63 for injection, 25.733± 3.899 for IUCD, 76.499± 3.482 for non using women and 91.219±5.001 pg/ml for virgin controls. Thus, they seems to be suitable as an assessment battery for immune status of women on contraception intake.
KEYWORDS:
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
REFERENCES
1.Finch CE, Kirkwood TBL , 2000, chance Development and Aging, Oxford University Press, Oxford, 3-111.
2.Clark W R, 1999, A Means to An End , The biological Basrs of aging and Death, Oxford University press, Oxford, 3-59.
3.Siegrist C-A, Aspinall R. B cell responses to vaccination at the extremes of age, Nature Reviews Immunology,2009;9 : 185-194.
4.Shnawa IMS, 2014, Immunology of Ageing and Longevity IISTE publications, USA.
5.Salaman J R, Sampson D, 1950. Clinical Immuosuppression, Grume and Stratton, New York.
6.Parslow T G, Stites D P, Terr AI, Imbodca JB, 2001, Medical Immunology 10th ed. Large Medical Books, McGraw-Hill, New York, 714-760.
7.Andrcani D, Bompiani G, DiMario U, Faulk WP, Galluzzo A, 1996, Immunobiology of Normal and Diabetic pregnancy, John Wiley and Sons, New York.
8.Theze J, 1998, The cytokine network and Immune Functions, Oxford University Press.
9.Cruse J M, Lewis RE, 1993. Complement today Karger, New York.
10.Ildgruben A K, Sjaberg I M, Marie-Louise K C. Influences of Hormoral contraceptives on the immune cells and thickness of humane vaginal epithelium, Obest Gynecol 2003;102 (3): 571-582.
11.Stevens C D, 2010, Clinical Immunology and serology: A laboratory perspective 3rd ed. F. A. Davis, philadelphia.
12.Mancini G, Carbonara A O, Here mans J F. Immunochemical quantation of antigen by single radial Immunodiffusion , Immunochem1995;2:235-245.
13.Mahdi B M. Role of some cytokine on reproduction, Med. East Fertil. Soc J 2001;16(5):220-223.
14.Red J. G, Simpson N A, Walker R G, Ecomidon O, Shillito J , Gooi H C, Duffy S R, Walker JJ. The carriage of Proinflammatary cytokine gene polymorphism in recurrent pregnancy loss. Am. Rep. Immunol 2001; 45:35-40.
15.Nasu K, Mastasiu N, Narahara H, Tanka Y, Miyakama I. Effect of interferon gamma on cytokine production by endometrial Stromal cells, Human reproduction 1998;13(9): 2598-2601.
16.Adashi E Y. Cytokine mediated regulation of ovarian function. Endocrinol 1989;124: 2043-2045.
17.Spron M B, Roberts A B, 1988, Peptide growth factors are multifunctional Nature 332: 217-219.
18.Clark D A, 1990. Lyrnphokines and cytokines affecting reproductive outcome in Andreani D Bompiani G, DiMario U, Faulk N P, Galluzzt A, Immunobiolgy of Normal and diabetic pregnancy, John Wiley and sons New York, 79-90.
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 AL-Bayati LAA. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.