DocumentsDate added
Original article
Swathimutyam Pallerla, Bala Prabhakar*
Affiliation:
Department of Pharmaceutics, Shobhaben Pratapbhai Patel, School of Pharmacy & Technology Management SVKM’S NMIMS, V.L. Mehta Road, Vile Parle (W), Mumbai – 400056 India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Pharmaceutics, Shobhaben Pratapbhai Patel, School of Pharmacy & Technology Management SVKM’S NMIMS, V.L. Mehta Road, Vile Parle (W), Mumbai – 400056 India
Address reprint requests to
*Dr. Bala Prabhakar.
Associate Dean & Senior Professor
Department of Pharmaceutics, Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management SVKM’S NMIMS, V.L. Mehta Road, Vile Parle (W), Mumbai – 400056
Article citation: Pallerla, S.,Prabhakar, B. Development and validation of Temozolomide solid lipid nanoparticle formulation by RP-HPLC method. J Pharm Biomed Sci. 2015;05(09):745-749. Available at www.jpbms.info
ABSTRACT: The present study was designed to develop newer method forsolid lipid nanoparticles with Temozolomide as anti-cancer drug. The method was carried out using C-18 kromasil (ODS column 250mm × 4.6 mm I.D.; particle size 5μm with mobile phase consisting of methanol: 0.5% glacial acetic acid in the ratio (20:80). The flow rate at 1.0 ml/min and effluent was detected at 316 nm. The retention time of temozolomide was observed at 6.32 minutes. The method was validated for specificity, accuracy, precision, linearity, and limit of detection, limit of quantification, robustness and solubility. LOD and LOQ of solid lipid nanoparticles temozolomide were 0.8µg/ml and 2µg/ml respectively. The calibration curve was linear in the concentration range of 10-70 μg/ml with coefficient correlation of 0.999. The percentage recovery for the temozolomide was 99.8% and % RSD was less than 1 %. There are scanty reports with relation to determination of temozolomide in solid lipid nanoparticle formulation. The proposed method was used for quantitative determination of temozolomide in solid lipid nanoparticles and is validated using various parameters.
KEYWORDS: Temozolomide, anti-cancer drug, methanol,0.5% glacial acetic acid, solid lipid nanoparticles, HPLC; validation.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Majority of the information gathered are from media sources which don’t reflect the author’s own opinion.
Copyright © 2015 Pallerla, S., Prabhakar, B. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
S. Jayashree† and N. Thangaraju†*
Affiliation:
†CAS in Botany, University of Madras, Chennai – 600 025, India
The name of the department(s) and institution(s) to which the work should be attributed:
University of Madras, Chennai – 600 025, India
Address reprint requests to
* N. Thangaraju.
CAS in Botany, University of Madras, Chennai – 600 025, India
Article citation: Jayashree, S., Thangaraju, N. Biosynthesis and characterization of silver nanoparticles using marine macro algae Sargassum plagiophyllum C.Agardh. J Pharm Biomed Sci. 2015; 05(09):705-712. Available at www.jpbms.info
ABSTRACT: “Nanotechnology” pact with the process that takes place on nanometer scale ranging approximately from 1–100nm. The properties of metal nano particles are entirely different from those of bulk materials from the similar atoms. The proportional justification in the development and synthesis of reliable and eco-friendly metal nanoparticles is significantly important in bio-nanotechnology. In the present work, we report the conditions for extracellular biosynthesis of silver nanoparticles (SNPs) using Sargassam plagiophyllum an endemic Hawaiian species common on wave swept benches. The synthesized SNPs were characterized by analytical techniques like UV-Visible spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR) and Dynamic Light Scattering (DLS). The crystalline nature of the SNp was analyzed by Powder X-ray diffraction, Transmission Electron Microscopy (TEM). The antibacterial activity was carried out using the bacterial pathogens Escherichia coli, Proteus vulgaris, Proteus mirabilis, Pseudomonas aureus, Bacillus subtilis, Staphylococcus aureus, Vibrio cholera and Enterococcus aerogens. The results showed that the morphological feature of the eco-friendly biosynthesized SNPs is spherical in shape and size range from 15–24 nm. Further, the antibacterial activity of synthesized silver nanoparticles showed effective inhibitory activity against the pathogens.
KEYWORDS: Biosynthesis; Silver Nanoparticles; Sargassam plagiophyllum.
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Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Copyright © 2015 Jayashree, S., Thangaraju, N.. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Famurewa Ademola C1,*, Kanu Shedrach C1, Uzoegwu Peter N2, Ogugua Victor N2
Affiliation:
1Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria, PMB 1010 Abakaliki, Nigeria
2Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria
The name of the department(s) and institution(s) to which the work should be attributed:
1.Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria, PMB 1010 Abakaliki, Nigeria
2.Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria
Address reprint requests to
* Famurewa, Ademola Clement.
Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Federal University, Ndufu-Alike, Ikwo, Ebonyi State
Article citation: Famurewa, A.C., Kanu, S.C., Uzoegwu, P.N., Ogugua, V.N. Ameliorative effects of hibiscus sabdariffa extract against carbon tetrachloride-induced lipid peroxidation, oxidative stress and hepatic damage in rats. J Pharm Biomed Sci. 2015;05(09):725-732. Available at www.jpbms.info
ABSTRACT: Objective: Hibiscus sabdariffais a medicinal plant associated with beneficial health effects. The leaves and flowers are used as traditional drinks and medicines in countries. The current study aimed to investigate Hibiscus sabdariffa Linn methanolic extract ameliorative potential against carbon tetrachloride-induced lipid peroxidation, hepatic damage and oxidative stress.
Methods: Adult male Wistar rats were administered 2.5ml/kg body weight of CCl4 by oral gavage to induce oxidative stress 48 hours before administration of 200, 600 to 1000mg/kg doses of Hibiscus sabdariffa methanolic extract to group 3, 4 and 5, respectively for 10 days (n=5). Group 1 was used as negative control, while group 2 was used as positive-comparative control (2.5ml/kg CCl4). At the end of the experiment, serum glutathione (GSH), vitamin C and E, MDA, liver damage markers and antioxidant enzymes were analysed in all the groups.
Results: Carbon tetrachloride-induced oxidative stress in experimental rats was evidenced by increase in malondialdehyde (MDA) and reduction in SOD, catalase and reduced glutathione (GSH).Hibiscus sabdariffa extract treatment at 600 and 1000mg/kg doses resulted in significant modulation of antioxidant indices and alkaline phosphatase (p<0.05), but failed to demonstrate significant effects in AST, ALT and MDA. There were significant increases (p<0.05) in the serum vitamin C and E at 600 and 1000mg/kg doses of the extract.
Conclusion: The overall results suggest that hibiscus sabdariffa contains bioactive phytochemicals that may improve hepatic status and ameliorate oxidative damage at high doses in carbon tetrachloride intoxication.
KEYWORDS: Hibiscus sabdariffa; hepatic damage; lipid peroxidation; oxidative stress; phytochemicals.
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Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Majority of the information gathered are from media sources which don’t reflect the author’s own opinion.
Original article
Ritu Gupta1,*, Varsha Porwal1,€, Sanjay Kumar Porwal1,†,Madhusudan Swarnkar1,ß,
Rishi Diwan1,¥, Sanjeev Gupta1,≠.
Affiliation:
1,*Associate Professor, Department of Obstetrics and Gynecology, 1,€Demonstrator, Department of Anatomy, 1,†Associate Professor, Department of General Surgery, 1,ßAssociate Professor, Department of P.S.M, 1,¥Professor, Department of Pathology,1,≠Senior Resident, Department of Anesthesia, Jhalawar Medical College and Hospital Society, Jhalawar (Rajasthan), India
The name of the department(s) and institution(s) to which the work should be attributed:
Jhalawar Medical College and Hospital Society, Jhalawar (Rajasthan), India
Address reprint requests to
* Dr. Sanjay Kumar Porwal.
B-4 Anand Vihar, Jhalawar (Rajasthan), 326001
ABSTRACT: Background: Perimenopause is the menstrual transitional interval in which a woman’s body makes a natural shift towards menopause.The histopathology of endometrial samples is a must in work up of all cases of perimenopausal women with abnormal uterine bleeding (AUB).
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Majority of the information gathered are from media sources which don’t reflect the author’s own opinion.
Copyright © 2015 Gupta, R.,Porwal, V.,Porwal, S.K.,Swarnkar, M.,Diwan,R.,Gupta S. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.