DocumentsDate added
Original article
Moulshree Kohli1,Puneet Ahuja2, Amit Gupta3*
1 Post graduate student, Department of Oral and Maxillofacial Pathology and Microbiology, I.T.S. Dental College, Hospital and Research Centre, Greater Noida, Uttar Pradesh, India
2 Prof & Head, Department of Oral and Maxillofacial Pathology and Microbiology, I.T.S. Dental College, Hospital and Research Centre, Greater Noida, Uttar Pradesh, India
3Senior Lecturer, Department of Oral and Maxillofacial Pathology and Microbiology, I.T.S. Dental College, Hospital and Research Centre, Greater Noida, Uttar Pradesh, India
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*Dr. Amit Gupta,
Department of Oral and Maxillofacial Pathology and Microbiology, ITS Dental College, Hospital and Research Centre, Greater Noida, Uttar Pradesh, India
Article citation: Kohli M, Ahuja P, Gupta A. EVALUATION OF DIFFERENT DECALCIFYING AGENTS ON ORAL HARD TISSUES:A COMPARATIVE STUDY.J Pharm Biomed Sci 2015;05(10):804–806. Available at www.jpbms.info
Introduction Decalcification of bone and teeth is often an essential and important step during tissue processing. The rate of decalcification and the effect of various decalcifying agents on the tissue and their staining characteristics are two important parameters influencing the selection of decalcifying solutions. Some decalcifying agents, although they completely and rapidly remove the calcium ions also adversely affect the staining characteristics and may cause damage to the organic components. This study aimed to EVALUATE the efficacy of the commonly used demineralizing agents to identify the best decalcifying agent. Materials and Methods Three decalcifying agents namely, 10 percent nitric acid, 10 percent hydrochloric acid and 10 percent formic acid were used to decalcify 30 natural teeth.The endpoint of decalcification was EVALUATED by physical and chemical methods. The decalcified teeth were subjected to routine processing and staining with hematoxylin and eosin stains.
Result Formic acid of 10% was the most considerate to the hard tissues and 10% hydrochloric acid was the least considerate to the tooth structure.
Conclusion Formic acid of 10% though being the slowest decalcifying agent, gave excellent results for soft-tissue integrity and staining characteristics.
KEYWORDS 10% nitric acid, 10% hydrochloric acid, 10% formic acid, decalcifying agents
Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None.
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents, and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Original article
Megha Pawar*, Rabindra N. Misra, Nageswari R. Gandham, Kalpana Angadi, Savita Jadhav,Chanda Vyawahare, Swarupa Hatolkar
Department of Microbiology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pune 411018, Maharashtra, India
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*Megha Pawar,
Research scholar,
Department of Microbiology
Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune 411018,Maharashtra, India
Article citation: Pawar M, Misra RN,Gandham NR, Angadi K, Jadhav S,Vyawahare C, Hatolkar S. PREVALENCE AND ANTIFUNGAL SUSCEPTIBILITY PROFILE OF CANDIDA SPECIES ISOLATED FROM TERTIARY CARE HOSPITAL, INDIA. J Pharm Biomed Sci 2015;05(10):812–816.Available at www.jpbms.info
Abstract
Background The change in epidemiology and antifungal susceptibility has generated interest among clinical microbiologists in the identification of Candida up to species level along with antifungal susceptibility patterns. Objectives This study was undertaken to INVESTIGATE the PREVALANCE of Candida spp and its antifungal susceptibility from the various clinical samples.Materials and Methods Total of 317 clinical samples from various suspected fungal infections were received in the Department of Microbiology to investigate suspected Candidal infections. Isolation, identification and antifungal susceptibility were done by standard conventional methods.Results A total of 104 non-duplicate Candida species were isolated, out of which 37 (35.6%) were C. albicans and 67 (64.4) were non-C. albicans, and the remaining were C. tropicalis 35 (33.7%), C. glabrata 18 (17.3%) and C. parapsilosis 6 (5.8%). Maximum isolates were from sputum as well as body fluids followed by pus, tissue and urine. Antifungal susceptibility showed higher degree of resistance to Fluconazole (4%), followed by Clotrimazole (2%) and lower degree to Amphotericin B (1%). All isolates were susceptible to Ketoconazole, Voriconazole and Nystatin.
Conclusion An increase in the infections was caused by non-candida albicans species. Candida species identification and antifungal susceptibility are important for the treatment especially for those who hospitalise with serious underlying disease.
KEYWORDS antifungal susceptibility testing, Candida species, CHROM agar, Sabouraud’s dextrose agar (SDA)
Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None.
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research,patents, and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Original article
Subrat Chandra1, Sushil Kumar Shukla2*,Dushyant Gaur3 Pooja Gupta4
1Associate Professor, Department of Pathology, Ram Manohar Lohia Institute of Medical Sciences, Vibhuti Khand, Gomti Nagar, Lucknow, India
2Associate Professor, Department of Community Medicine, UP RIMSR, Saifai, Etawah, UP, India
3 Professor & Head, Department of Pathology, HIMS, Dehradun, India
4 Associate Professor, Department of Obs. & Gyn., UP RIMSR, Saifai, Etawah, UP, India
Address reprint requests to
*Dr. Sushil Kumar Shukla,
Associate Professor, Department of Community Medicine, UP RIMSR, Saifai,Etawah, UP, India
Article citation: Chandra S, Shukla S, Gaur D, Gupta P. A cross-sectional study to identify the proportion of anaplastic carcinoma in thyroid cancer cases reporting to HIMS, Dehradun, India. J Pharm Biomed Sci 2015;05(10):817–821.Available at www.jpbms.info
Abstract
Background Anaplastic carcinoma of the thyroid (ATC) is the most aggressive thyroid gland malignancy. Although ATC accounts for less than 2% of all thyroid cancers, it causes up to 40% of deaths from thyroid cancer. Anaplastic carcinoma consists partly or entirely of undifferentiated cells. This represents 10% of all thyroid carcinomas and this is the most aggressive neoplasm of this gland.
Objective To estimate the proportion of anaplastic carcinoma and its subtypes. Methods and Results We studied 150 cases out of which 15 were reported as thyroid malignancy. Anaplastic carcinomas are reported here in four cases of middle age 5th and 6th decay of their life, two male and two female 1:1 with history suggestive of hyperthyroidism in three cases and euthyroid in one case. The tumours were of variable size and showed large pleomorphic cells in clusters as well as in an isolated form. The femaleto- male ratio was approximately 3:1. Peak incidence occurs during the third to fourth decades of life. The age range of affected patients reportedly is 15–70 years.
Conclusion ATC is a very uncommon malignancy but in our study we found four cases and we confirmed it by histopathological examination.
KEYWORDS anaplastic carcinoma, thyroid cancer, neoplastic lesions, hyperthyroidism, euthyroid
Statement of originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None.
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research,patents, and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.