Property | Value |
Name | Serum Amino Acids Profiling In NAFLD Mice |
Description | Research article
ABSTRACT: Objectives: To detect the variation of serum amino acids in control and nonalcoholic fatty liver disease (NAFLD) mice by PITC pre-column derivatization HPLC, and further explore the potential biomarkers associated with the development of NAFLD using metabolism profiling analysis. Methods: Male C57BL/6J mice were fed with high fat and high fructose diet, after 8 weeks, serum samples of control and model mice were collected. Serum biochemical parameter and liver histopathology were examined, and then metabolic profile was further analyzed using PITC pre-column derivatization HPLC. Result: Levels of serum and hepatic biochemical parameter and liver histopathology verified the NAFLD model successfully. The metabolism profile of NAFLD model mice was significantly different from that of the control mice. Conclusion: Pattern recognition method demonstrated that there were significant differences in the five amino acids markers (glutamic acid, glycine, methionine, isoleucine, valine) between the control and NAFLD mice. Amino acids metabolism profile combined with pattern recognition technology may reflect to a certain extent the metabolism changes of NAFLD mice. It also provided the potential scientific and clinic value for further study of NAFLD.
ACKNOWLEDGEMENTS: The work was supported by grants of the National Natural Science Foundation of China (No. 21172271), Youth fund project of national natural science fund (No.81202454), Major Scientific and Technological Special Project of Administration of Ocean and Fisheries of Guangdong Province (A201301C08), and from Natural Science Foundation of Guangdong Provincial, China (No. 2011020001231). |
Filename | Xiang Qi(355-364.).pdf |
Filesize | 982.41 kB |
Filetype | pdf (Mime Type: application/pdf) |
Creator | admin |
Created On: | 04/09/2015 00:00 |
Viewers | Everybody |
Maintained by | Editor |
Hits | 2197 Hits |
Last updated on | 05/12/2015 05:38 |
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