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Name | Comparison of antimicrobial efficacy of Propolis, Azadirachta indica (Neem), Melaleuca alternifolia (Tea tree oil), Curcuma longa (Turmeric) and 5% Sodium hypochlorite on Candida albicans biofilm formed on tooth substrate: An In-vitro study |
Description | Original article Materials and methods: Extracted human mandibular premolars were biomechanically prepared, vertically sectioned, placed in tissue culture wells exposing the root canal surface to C. albicans grown on Sabouraud Dextrose Agar to form a biofilm. At the end of 2 days, all groups were treated with test solutions and control for 10 min and evaluated for Candida growth and number of colony forming units were calculated. The readings were subjected to statistical analysis using ANOVA and Tukey’s post hoc test. Results: Sodium hypochlorite and propolis groups exhibited highest antimicrobial efficacy against C. albicans with no statistically significant difference. It was followed by Melaleuca alternifolia (Tea tree oil), Curcuma longa(turmeric) group. A.india (Neem) had limited antifungal action followed by the negative control group of saline. Conclusion: According to the results of this study, propolis can be used as an effective antifungal agent similar to that of sodiumhypochlorite, although long-term in vivo studies are warranted. References:
1.Saleh IM, RytlerLE, HaapasaloD. Survival of Enterococcus faecalis in infected dentinal tubules after root canal filling with different root canal sealers: in vitro study. J Endod 2004 37;193-198.[PubMed] 2 Lee Y1, Han SH, Hong SH, Lee JK, Ji H, Kum KY. Antimicrobial efficacy of a polymeric chlorhexidine release device using in vitro model of Enterococcus faecalis dentinal tubule infection. J Endod 2008;34:855-858.[PubMed] 3.Hess W. Anatomy of root canals in the teeth of permanent dentition. New York: William Wood & co.1925: 82-85. 4.Baumgartner JC, Watts CM, Xia T. Occurrence of Candida albicans in infections of endodontic origin. J Endod 2000;26 :695-698.[PubMed] 5.Siqueira JF Jr., Rôças IN. Diversity of endodontic microbiota revisited. J Dent Res 2009;88: 969-981. doi: 10.1177/0022034509346549.[PubMed] 6.Waltimo TM, Haapasalo M, Zehnder M, Meyer J. Clinical aspects related to endodontic yeast infections. Endod Topics2004;9:66-78. 7.Spangberg L, Engström B, Langeland K. Biologic effects of dental materials. 3. Toxicity and antimicrobial effect of endodontic antiseptics in vitro. Oral Surg Oral Med Oral Pathol 1973;36: 856-871.[Pubmed] 8.McComb D, Smith DC.A preliminary scanning electron microscopic study of root canals after endodontic procedures. J Endod1975; 1:238-242.[PubMed] 9.Mohammadi Z. Sodium hypochlorite in endodontics: An update review. Int Dent J 2008; 58: 329-341.[PubMed] 10.Vermani K, Garg S. Herbal medicines for sexually transmitted diseases and AIDS. J Ethnopharmacol 2002; 80: 49-66.[PubMed] 11.Hyun Koo, Pedro L. Rosalen, Jaime A. Cury, Yong K. Park, and William H. Bowen. Effects of Compounds Found in Propolis on Streptococcus mutans Growth and on Glucosyltransferase activity. Antimicrob Agents Chemother2002 ;46: 1302-1309. 12.OzantOncag, Dilsah Cogulu, AtacUzel, KadriyeSorkun. Efficacy of propolis as an intracanal medicament against Enterococcus faecalis. Gen Dent 2006;.54: 319-322.[Pubmed] 13.Subapriya R, Nagini S. Medicinal properties of neem leaves: A review. Curr Med Chem Anticancer Agents 2005; 5:149-156.[PubMed] 14.Sadr Lahijani MS, RaoofKateb HR, Heady R, Yazdani D. The effect of German chamomile [Marticariarecutitia L.] extract and tea tree [ Melaleucaalternifolia L.] oil used as irrigants on removal of smear layer: a scanning electron microscopy study. IntEndod 2006; 39:190-195.[PubMed] 15.Chaturvedi TP. Uses of turmeric in dentistry –an update. Indian J Dent Res 2009; 20:107-109.[PubMed] 16.NeelakantanP, Subbarao C, Subbarao CV. Analysis of Antibacterial Activity of Curcumin against Enterococcus faecalis. Int J Curr Res Rev 2011; 3: 37-42. 17.Shingare P, Chaugule V. Comparative evaluation of antimicrobial activity of miswak, propolis, sodium hypochlorite and saline as root canal irrigants by microbial culturing and quantification in chronically exposed primary teeth. Germs 2011;1:12-21.[PubMed] 18.Irshad S , Butt M, Younis H. In-Vitro antibacterial activity of two medicinal plants Neem (Azadirachta indica) and Peppermint Intl. R. J. of Pharmaceuticals 2011;1: 9-14. 19.Garg P, Tyagi SP, Sinha DJ, Singh UP, Malik V, Maccune ER. Comparison of antimicrobial efficacy of propolis, Morindacitrifolia, Azadirachta indica, triphala, green tea polyphenols and 5.25% sodium hypochlorite against Enterococcus faecalis biofilm. Saudi Endod J 2014;4:122-7.[PDF] 20.JhaHarit, Anand Barapatre, Mithlesh Prajapati, Keshaw Ram Aadil and Sunil Senapati. Antimicrobial Activity of rhizome of selected Curcuma Variety. Int J Life Sc Bt & Pharm Res 2013; 2:183-189. 21.Uday Kamath, Hina Sheth, Sai Ramesh, Keshav Singla. Comparison of the Antibacterial Efficacy of Tea Tree Oil with 3% Sodium Hypochlorite and 2% Chlorhexidine against E. faecalis: An in vitro Study. J Contemp Dent 2013 ;3: 117-20.[PDF] 22.Pujar M, Patil C, Kadam A. Comparison of antimicrobial efficacy of triphala, green tea polyphenols and 3% of sodium hypochlorite on Enterococcus faecalis biofilms formed on tooth substrate: In vitro. JIOH 2011; 3: 23-29.[PDF] 23. Prabhakar J, Senthilkumar M, Priya MS, Mahalakshmi K, Sehgal PK, Sukumaran VG.Evaluation of antimicrobial efficacy of herbal alternatives (Triphala and green tea polyphenols), MTAD, and 5% sodium hypochlorite against Enterococcus faecalis biofilm formed on tooth substrate: an in vitro study.J Endod. 2010 Jan;36(1):83-6. doi: 10.1016/j.joen.2009.09.040. [PubMed] 24.Baillie GS,DouglasLJ.Role of dimorphism in the development of Candida albicans biofilms. J Med Microbiol 1999;48: 671-679.[PubMed] 25.Nikawa H, Nishimura H, Hamada T, Makihira S, Samaranayake LP. Relationship between thigmotropism and candida biofilm formation in vitro. Mycopathologia 1998;144:125-129.[PubMed] 26.Davies JM, Stacey AJ, Gilligan CA. Candida albicanshyphal invasion: Thigmotropism or chemotropism? FEMS Microbiol Lett1999;171:245-249.[PubMed] 27.Ayhan H, Sultan N, Cirak M, Ruhi MZ, Bodur H. Antimicrobial effects of various endodontic irrigants on selected microorganisms. IntEndod J 1999;32: 99-102.[PubMed] 28. Vinothkumar TS, Rubin MI, Balaji L, Kandaswamy D. In vitro evaluation of five different herbal extracts as an antimicrobial endodontic irrigant using real time quantitative polymerase chain reaction. J Conserv Dent 2013;16:167-70.[HTML] 29.Takaisi-Kikuni NB, Schilcher H. Electron microscopic and microcalorimetric investigations of the possible mechanism of the antibacterial action of a defined propolis provenance. Planta Med 1994;60:222-227.[PubMed] 30.Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol 1999; 64:235-240.[PubMed] 31.Ivančajić S, Mileusnić I, Milošević DC. In vitro antibacterial activity of propolis extracts on 12 different bacteria in conditions of 3 various pH values. Arch BiolSci Belgrade 2010;62:915-934. 32.Tyagi SP, Sinha DJ, Garg P, Singh UP, Mishra CC, Nagpal R. Comparison of antimicrobial efficacy of propolis, Morindacitrifolia, Azadirachtaindica(Neem) and 5% sodium hypochlorite on Candida albicansbiofilm formed on tooth substrate: An in-vitro study. J Conserv Dent. 2013 Nov;16(6):532-5. doi: 10.4103/0972-0707.120973.[PubMed] 33.Polaquini SR, Svidzinski TI, Kemmelmeier C, Gasparetto A. Effect of aqueous extract from neem (Azadirachtaindica A. Juss) on hydrophobicity, biofilm formation and adhesion in composite resin by Candida albicans. Arch Oral Biol 2006;5:482-490.[PubMed] 34.Nayak A, Nayak RN, Soumya GB, Bhat K, Kudalkar M. Evaluation of antibacterial and anticandidal efficacy of aqueous and alcoholic extract of neem (Azadirachta indica): An in vitro study. IJRAP 2011;2: 230-235. 35.Bohora A, Hedge V, Kokate S. Comparison of the antibacterial efficiency of neem leaf extract and 2% sodium hypochlorite against E. faecalis, C.albicans and mixed culture - An in vitro study. Endodontology 2010; 22: 8-12. 36.Cox, S. D., C. M. Mann, and J. L. Markham. Interactions between components of the essential oil of Melaleucaalternifolia. J. Appl. Microbiol 2001;.91:492–497.[PubMed] 37.D’Auria, F. D., L. Laino, V. Strippoli, M. Tecca, G. Salvatore, L. Battinelli,and G. Mazzanti. In vitro activity of tea tree oil against Candida albicans mycelial conversion and other pathogenic fungi. J. Chemother 2001;13:377–383.[PubMed] 38.Hammer, K. A., C. F. Carson, and T. V. Riley. Melaleuca alternifolia (tea tree) oil inhibits germ tube formation by Candida albicans. Med. Mycol 2010;38:355–362.[PubMed] 39.Nadia G, Talat Y M, Nayyar J, Samia A. Studies On The Antibacterial Effect Of Different Fractions Of Curcuma Longa Against Urinary Tract Infection Isolates. Pak J Bio Sci. 2004; 7: 2055-2060.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work. |
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