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Name | Molecular Characterization of the Nettle Plant Urtica parviflora Based On RAPD Marker |
Description | Original research article:- Goel Chirag, Verma Pankaj, Ahmad Naseer, Nailwal K Tapan* Department of Biotechnology, Kumaun University, Nainital, Campus, Bhimtal - 263136, Uttarakhand, India.
Abstract:-Urtica parviflora is considered as an important Medicinal plant, due to its various ethanomedical uses. Here, we analyze the Genetic Variation in U.parviflora, with respect to plant distribution in Kumaun hills based on change in altitude. Examination of Random amplified Polymorphic DNA (RAPD) markers from four plant samples collected at different heights from sea level indicated that genetic variation was appreciable, as samples from lower altitudes showed low genetic similarity with samples collected from higher altitudes. A total of 70 scorable bands were produced in four samples with 8 primers. The average number of bands per primer was 8.75. Out of 70 bands, 48 bands were polymorphic (68.75%) noted in the present investigation. The dendrogram of the samples showed two major clusters. The samples of Mukteshwar, Nainital and Bhowali are in one cluster and Bhimtal in other cluster.
Key words: Genetic Diversity, Primers, RAPD analysis, Taq DNA polymerase. |
Filename | Goel et.al..pdf |
Filesize | 145.23 kB |
Filetype | pdf (Mime Type: application/pdf) |
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Created On: | 04/18/2011 00:00 |
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Last updated on | 04/28/2011 07:29 |
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